MD11G1273700.v1.1

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012725
Physical Location & Seq
Forward (+)
39021776 .. 39023068
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1273700.v1.1.491

Sequence Viewer

Length: 345 bp
ATGGCTAAAGCAGCTCTCTCTGTTCTGCTCCTGCTCCTGCTCTTGTCCTTCACTTCAGAAACTTTGCTCATGGTAAATGGACAGAAAACTTGGTGTGTGGCCAGACCTTCGTCCGACCAAGCAACGCTCTTATCGAATCTGAATTACGCGTGCGCGCACGTAGATTGCCAGATTCTTCGAAAGGGATGCCCGTGCTCGTCGCCGGATAATCTCATGAACCGTGCCTCCATAGCCATGAACATGTATTACCAATCCAAGGGAAAGAACCAGTGGAATTGTGACTTTAGAGGCTCTGCTCTCATTGTCGTGACTGATCCAAGTTATGGTGACTGCATCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

115

Amino Acids

12.52

Weight (kDa)

8.18

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 30 - 99 4.3e-19 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78520 AT2G43670
fragaria_vesca FvH4_3g17340
malus_domestica MD11G1273700.v1.1
prunus_persica Prupe.4G153400_v2.0.a1
pyrus_communis pycom11g24140
rosa_chinensis RchiOBHm_Chr5g0028861
rosa_laevigata RLG00000033120
rosa_multiflora Rmu_sc0005178.1_g000012
rosa_roxburghii Rroxscaffold_1G00051020
rosa_rugosa Rorug05G0110800 Rorug05G0110900 Rorug05G0111000
rosa_samantha Rh5AG203300 Rh5BG201400 Rh5CG222400 Rh5DG205000
rosa_wichuraiana Rw5G018390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 323
AccII CGCG 2 cut(s) 149, 155
AclWI GGATC 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 39
AfiI CCNNNNNNNGG 2 cut(s) 256, 323
AflIII ACRYGT 2 cut(s) 147, 240
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 1 cut(s) 14
AluI AGCT 1 cut(s) 14
Alw21I GWGCWC 1 cut(s) 197
AlwI GGATC 1 cut(s) 308
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 11
AspLEI GCGC 2 cut(s) 155, 157
AsuHPI GGTGA 1 cut(s) 338
AsuII TTCGAA 1 cut(s) 178
BalI TGGCCA 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 23
BcgI CGANNNNNNTGC 2 cut(s) 168, 202
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
BmsI GCATC 1 cut(s) 176
BoxI GACNNNNGTC 1 cut(s) 109
Bpu14I TTCGAA 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 160
BsaJI CCNNGG 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 323
Bse1I ACTGG 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 1 cut(s) 191
BseLI CCNNNNNNNGG 2 cut(s) 256, 323
BseNI ACTGG 1 cut(s) 268
BsePI GCGCGC 1 cut(s) 153
BseXI GCAGC 1 cut(s) 23
Bsh1236I CGCG 2 cut(s) 149, 155
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 203
BslI CCNNNNNNNGG 2 cut(s) 256, 323
BsnI GGCC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 1 cut(s) 313
BspANI GGCC 1 cut(s) 101
BspFNI CGCG 2 cut(s) 149, 155
BspHI TCATGA 1 cut(s) 213
BspPI GGATC 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 178
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 255
BssHII GCGCGC 1 cut(s) 153
BssMI GATC 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 160
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 151, 155
BstF5I GGATG 1 cut(s) 191
BstFNI CGCG 2 cut(s) 149, 155
BstHHI GCGC 2 cut(s) 155, 157
BstKTI GATC 1 cut(s) 316
BstMBI GATC 1 cut(s) 313
BstMWI GCNNNNNNNGC 2 cut(s) 11, 230
BstNSI RCATGY 1 cut(s) 244
BstPAI GACNNNNGTC 1 cut(s) 109
BstUI CGCG 2 cut(s) 149, 155
BstV1I GCAGC 1 cut(s) 23
BsuRI GGCC 1 cut(s) 101
BtsCI GGATG 1 cut(s) 191
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 151, 155
CciI TCATGA 1 cut(s) 213
CfoI GCGC 2 cut(s) 155, 157
CviAII CATG 4 cut(s) 70, 214, 235, 241
CviJI RGCY 5 cut(s) 5, 14, 101, 233, 291
CviKI_1 RGCY 5 cut(s) 5, 14, 101, 233, 291
DpnI GATC 1 cut(s) 315
DpnII GATC 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 255
Eco57I CTGAAG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 255
EcoT22I ATGCAT 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 4 cut(s) 73, 217, 238, 244
FaiI YATR 8 cut(s) 71, 215, 230, 236, 242, 324, 339, 343
FatI CATG 4 cut(s) 69, 213, 234, 240
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 12
GlaI GCGC 2 cut(s) 154, 156
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 203
HhaI GCGC 2 cut(s) 155, 157
Hin1II CATG 4 cut(s) 73, 217, 238, 244
Hin6I GCGC 2 cut(s) 153, 155
HinP1I GCGC 2 cut(s) 153, 155
HinfI GANTC 2 cut(s) 136, 172
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 338
Hpy188I TCNGA 3 cut(s) 58, 115, 141
Hpy188III TCNNGA 2 cut(s) 214, 307
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 58, 117
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 2 cut(s) 333, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 230
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 4 cut(s) 73, 217, 238, 244
HspAI GCGC 2 cut(s) 153, 155
Kzo9I GATC 1 cut(s) 313
LmnI GCTCC 2 cut(s) 33, 39
LpnPI CCDG 6 cut(s) 44, 50, 115, 182, 216, 281
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 176
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 3 cut(s) 278, 307, 326
MalI GATC 1 cut(s) 315
MboI GATC 1 cut(s) 313
MboII GAAGA 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 197
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 2 cut(s) 142, 274
MluI ACGCGT 1 cut(s) 147
MluNI TGGCCA 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 2 cut(s) 235, 281
Mox20I TGGCCA 1 cut(s) 101
Mph1103I ATGCAT 1 cut(s) 343
MscI TGGCCA 1 cut(s) 101
MslI CAYNNNNRTG 3 cut(s) 233, 239, 305
Msp20I TGGCCA 1 cut(s) 101
MspI CCGG 1 cut(s) 203
MvnI CGCG 2 cut(s) 149, 155
MwoI GCNNNNNNNGC 2 cut(s) 11, 230
NdeII GATC 1 cut(s) 313
NlaIII CATG 4 cut(s) 73, 217, 238, 244
NmuCI GTSAC 3 cut(s) 278, 307, 326
NsiI ATGCAT 1 cut(s) 343
NspI RCATGY 1 cut(s) 244
NspV TTCGAA 1 cut(s) 178
PagI TCATGA 1 cut(s) 213
PauI GCGCGC 1 cut(s) 153
PciI ACATGT 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PfeI GAWTC 2 cut(s) 136, 172
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 160
PscI ACATGT 1 cut(s) 240
PshAI GACNNNNGTC 1 cut(s) 109
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
PteI GCGCGC 1 cut(s) 153
RseI CAYNNNNRTG 3 cut(s) 233, 239, 305
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 313
SduI GDGCHC 1 cut(s) 197
SetI ASST 3 cut(s) 16, 109, 162
SfaNI GCATC 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 178
SmiMI CAYNNNNRTG 3 cut(s) 233, 239, 305
Sse9I AATT 2 cut(s) 142, 274
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 1 cut(s) 162
TaqI TCGA 2 cut(s) 134, 178
TasI AATT 2 cut(s) 142, 274
TfiI GAWTC 2 cut(s) 136, 172
TscAI CASTG 1 cut(s) 275
TseFI GTSAC 3 cut(s) 278, 307, 326
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 3 cut(s) 278, 307, 326
TspDTI ATGAA 2 cut(s) 230, 251
TspRI CASTG 1 cut(s) 275
Van91I CCANNNNNTGG 1 cut(s) 323
XceI RCATGY 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.