Rorug05G0110900

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012725
Physical Location & Seq
Forward (+)
9896005 .. 9897365
1361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0110900.1

Sequence Viewer

Length: 1272 bp
ATGAACACCCAGAATGCCAGGTTGATTGGGTCTGAGATCGGGTCAGTTCTGGAGATAGAAGACCCCGACAAGGTTGATGCAACTCTTCATGGTTTCCTGAGAGTGAAGGTTCTCATGGATTCCAGAAACCCCTTCCCTACTGGTTTCTGGCTTCCTCTAAACAACTACACCTCAACCAGGGTCGACTACGCTTACGAAGGCCTCTGTGACTTCTGTTACAAATGTGGGCGGCTAGGGCACCACCTGGATGTCTGCAAGAATTTTAGACTTATCAACCTTGAGGAGGGTGAGCCGGAAAATATTTGGTACGGTCCATGGATGAAGAGGAAGCCCCTCTCTATCAAACAGCAATCTTGGCTTGAGCGGCCGACGAGGGTTTACCGGAAAAAGCCTAAAACAAGGAGGTCCAACGAGCTAGGGTTTGATTCCCCGTATGCATCACATGCAAGCACTAGCTACCAGCCCCAAGGCCACAGCTCTGAAACAACGTTGGCTGACAAGGATGTGACACGCCTTCTGATCTCTGAGCCTGATGATGAAGACCTGTCATCCCACAATTTAAATGTTCAAATCACTCCCGTGAGAAACCCCGGCACTTGTCACCTTTTGTCCCTTGGTCCACATGCTTTGCCTGTTACAGCCCCAAAGGTAACCCCTTTCCCCAGCCCCCATTGGCTAAAGGTGGCGGTCACCACGACCAAAACACATGTGGAGTGGACCAAGAGAGTCAACAATCTGTATCCACTCCCCCAAACCCCTGCAAACAGACAGAAACCCTCCCATACTAGTACCCACTATAGGCCAAAACCCGTACTCCCCCTCCCGCTACTTGGGCCTAGTCTGAAACCATTGAAGCCCAACCCGAGCTTCAGTGATTTGATTGTGGGCAGGCCTGTGCTTCCCCCTCTCACCCAGGACCCATTTAAAGGAAAAGGCCCGACCCTACTCTCCTACTCTTCATTTAATCAGGCTCCTTCCACCCAACTTCCACAATCTCAACCTATCAGCATTTCATGTGAACCCCCAGCTCCACTTCACTTCAACTCCCAAGCTCCTCCTCCAGCTACAGCTAAAAGAACCCGCTCACAAACCAACCCAACCGACTCTCTGAGACACTCTAAAACCCGTCTCATCACCGACCCATATCAGTCCTTTGATTCTCTGAAACATTTTGATCAATACACCCCAATCCCAGCCCCAACCATTTCAGAACCAGCCAGGGGAAGAGGTCGAGCTCAGGGAAGAGGAAGAAGATCGACCCAGTGGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

423

Amino Acids

47.5

Weight (kDa)

9.63

Isoelectric Point (pI)

53.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78520 AT2G43670
fragaria_vesca FvH4_3g17340
malus_domestica MD11G1273700.v1.1
prunus_persica Prupe.4G153400_v2.0.a1
pyrus_communis pycom11g24140
rosa_chinensis RchiOBHm_Chr5g0028861
rosa_laevigata RLG00000033120
rosa_multiflora Rmu_sc0005178.1_g000012
rosa_roxburghii Rroxscaffold_1G00051020
rosa_rugosa Rorug05G0110800 Rorug05G0110900 Rorug05G0111000
rosa_samantha Rh5AG203300 Rh5BG201400 Rh5CG222400 Rh5DG205000
rosa_wichuraiana Rw5G018390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 237
AccBSI CCGCTC 2 cut(s) 364, 1083
AccI GTMKAC 1 cut(s) 183
AciI CCGC 5 cut(s) 229, 364, 686, 824, 1081
AclI AACGTT 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 365
AcsI RAATTY 1 cut(s) 259
AcuI CTGAAG 1 cut(s) 853
AfaI GTAC 3 cut(s) 308, 790, 813
AfiI CCNNNNNNNGG 8 cut(s) 70, 177, 283, 798, 830, 926, 1220, 1266
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 3 cut(s) 569, 853, 1042
AhlI ACTAGT 1 cut(s) 785
AjnI CCWGG 5 cut(s) 17, 176, 243, 912, 1217
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AleI CACNNNNGTG 1 cut(s) 578
AluBI AGCT 9 cut(s) 415, 456, 477, 867, 1028, 1052, 1064, 1070, 1235
AluI AGCT 9 cut(s) 415, 456, 477, 867, 1028, 1052, 1064, 1070, 1235
Alw21I GWGCWC 1 cut(s) 1237
Alw26I GTCTC 2 cut(s) 1105, 1133
Ama87I CYCGRG 1 cut(s) 862
AoxI GGCC 7 cut(s) 199, 365, 469, 800, 833, 890, 934
ApoI RAATTY 1 cut(s) 259
AspS9I GGNCC 7 cut(s) 311, 405, 617, 717, 833, 916, 935
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 5 cut(s) 299, 593, 682, 901, 1126
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 5 cut(s) 311, 405, 617, 717, 916
BaeGI GKGCMC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 1237
BbsI GAAGAC 2 cut(s) 66, 546
Bbv12I GWGCWC 1 cut(s) 1237
BciT130I CCWGG 5 cut(s) 19, 178, 245, 914, 1219
BciVI GTATCC 1 cut(s) 750
BclI TGATCA 1 cut(s) 1174
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 2 cut(s) 1105, 1133
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 5 cut(s) 233, 416, 453, 786, 837
BfmI CTRYAG 2 cut(s) 796, 1065
BfuI GTATCC 1 cut(s) 750
BisI GCNGC 2 cut(s) 230, 365
BlsI GCNGC 2 cut(s) 231, 366
Bme1390I CCNGG 6 cut(s) 19, 178, 245, 591, 914, 1219
Bme18I GGWCC 5 cut(s) 311, 405, 617, 717, 916
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 7 cut(s) 311, 405, 617, 717, 833, 916, 935
BmiI GGNNCC 3 cut(s) 239, 918, 972
BmrFI CCNGG 6 cut(s) 19, 178, 245, 591, 914, 1219
BmrI ACTGGG 1 cut(s) 1255
BmsI GCATC 2 cut(s) 67, 446
BmuI ACTGGG 1 cut(s) 1255
BpiI GAAGAC 2 cut(s) 66, 546
BpmI CTGGAG 2 cut(s) 71, 1044
Bpu10I CCTNAGC 1 cut(s) 1236
BpuEI CTTGAG 2 cut(s) 299, 380
BpuMI CCSGG 1 cut(s) 591
BsaJI CCNNGG 7 cut(s) 177, 314, 466, 589, 613, 912, 1218
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 6 cut(s) 798, 804, 828, 834, 1028, 1058
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 177, 283, 798, 830, 926, 1220, 1266
Bse1I ACTGG 2 cut(s) 145, 1261
BseBI CCWGG 5 cut(s) 19, 178, 245, 914, 1219
BseDI CCNNGG 7 cut(s) 177, 314, 466, 589, 613, 912, 1218
BseGI GGATG 4 cut(s) 253, 324, 508, 548
BseLI CCNNNNNNNGG 8 cut(s) 70, 177, 283, 798, 830, 926, 1220, 1266
BseMII CTCAG 5 cut(s) 24, 89, 516, 1100, 1250
BseNI ACTGG 2 cut(s) 145, 1261
BseRI GAGGAG 3 cut(s) 296, 1044, 1047
BseSI GKGCMC 1 cut(s) 240
BseX3I CGGCCG 1 cut(s) 365
BseYI CCCAGC 3 cut(s) 662, 1024, 1192
Bsh1285I CGRYCG 1 cut(s) 368
BshFI GGCC 7 cut(s) 201, 367, 471, 802, 835, 892, 936
BshNI GGYRCC 1 cut(s) 237
BsiEI CGRYCG 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 1237
BsiHKCI CYCGRG 1 cut(s) 862
BsiSI CCGG 3 cut(s) 293, 382, 591
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 8 cut(s) 70, 177, 283, 798, 830, 926, 1220, 1266
BsmAI GTCTC 2 cut(s) 1105, 1133
BsmBI CGTCTC 1 cut(s) 1133
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 19
BsnI GGCC 7 cut(s) 201, 367, 471, 802, 835, 892, 936
BsoBI CYCGRG 1 cut(s) 862
Bsp1286I GDGCHC 2 cut(s) 240, 1237
Bsp143I GATC 4 cut(s) 36, 519, 1174, 1253
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 5 cut(s) 229, 364, 686, 824, 1081
BspANI GGCC 7 cut(s) 201, 367, 471, 802, 835, 892, 936
BspCNI CTCAG 5 cut(s) 25, 90, 517, 1101, 1249
BspLI GGNNCC 3 cut(s) 239, 918, 972
BspT107I GGYRCC 1 cut(s) 237
BsrBI CCGCTC 2 cut(s) 364, 1083
BsrI ACTGG 2 cut(s) 145, 1261
BssECI CCNNGG 7 cut(s) 177, 314, 466, 589, 613, 912, 1218
BssMI GATC 4 cut(s) 36, 519, 1174, 1253
BssT1I CCWWGG 3 cut(s) 314, 466, 613
Bst2UI CCWGG 5 cut(s) 19, 178, 245, 914, 1219
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 5 cut(s) 90, 317, 961, 1219, 1237
BstAPI GCANNNNNTGC 1 cut(s) 443
BstC8I GCNNGC 2 cut(s) 448, 890
BstDEI CTNAG 5 cut(s) 33, 98, 525, 1109, 1236
BstDSI CCRYGG 1 cut(s) 314
BstEII GGTNACC 2 cut(s) 649, 688
BstENI CCTNNNNNAGG 2 cut(s) 175, 281
BstF5I GGATG 4 cut(s) 253, 324, 508, 548
BstKTI GATC 4 cut(s) 39, 522, 1177, 1256
BstMAI GTCTC 2 cut(s) 1105, 1133
BstMBI GATC 4 cut(s) 36, 519, 1174, 1253
BstMCI CGRYCG 1 cut(s) 368
BstMWI GCNNNNNNNGC 5 cut(s) 235, 355, 364, 443, 832
BstNI CCWGG 5 cut(s) 19, 178, 245, 914, 1219
BstNSI RCATGY 3 cut(s) 446, 626, 710
BstPI GGTNACC 2 cut(s) 649, 688
BstSCI CCNGG 6 cut(s) 17, 176, 243, 589, 912, 1217
BstSFI CTRYAG 2 cut(s) 796, 1065
BstSLI GKGCMC 1 cut(s) 240
BstV2I GAAGAC 2 cut(s) 66, 546
BstZI CGGCCG 1 cut(s) 365
BsuI GTATCC 1 cut(s) 750
BsuRI GGCC 7 cut(s) 201, 367, 471, 802, 835, 892, 936
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 4 cut(s) 253, 324, 508, 548
BtsIMutI CAGTG 2 cut(s) 877, 1268
Cac8I GCNNGC 2 cut(s) 448, 890
Cfr13I GGNCC 7 cut(s) 311, 405, 617, 717, 833, 916, 935
Csp6I GTAC 3 cut(s) 307, 789, 812
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviAII CATG 7 cut(s) 89, 115, 315, 443, 623, 707, 1014
CviQI GTAC 3 cut(s) 307, 789, 812
DdeI CTNAG 5 cut(s) 33, 98, 525, 1109, 1236
DpnI GATC 4 cut(s) 38, 521, 1176, 1255
DpnII GATC 4 cut(s) 36, 519, 1174, 1253
DraI TTTAAA 2 cut(s) 561, 925
EaeI YGGCCR 1 cut(s) 365
EagI CGGCCG 1 cut(s) 365
Eam1104I CTCTTC 5 cut(s) 90, 317, 961, 1219, 1237
EarI CTCTTC 5 cut(s) 90, 317, 961, 1219, 1237
Ecl136II GAGCTC 1 cut(s) 1235
EclXI CGGCCG 1 cut(s) 365
Eco130I CCWWGG 3 cut(s) 314, 466, 613
Eco147I AGGCCT 2 cut(s) 201, 892
Eco24I GRGCYC 1 cut(s) 1237
Eco47I GGWCC 5 cut(s) 311, 405, 617, 717, 916
Eco52I CGGCCG 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 1235
Eco57I CTGAAG 1 cut(s) 853
Eco88I CYCGRG 1 cut(s) 862
Eco91I GGTNACC 2 cut(s) 649, 688
EcoICRI GAGCTC 1 cut(s) 1235
EcoNI CCTNNNNNAGG 2 cut(s) 175, 281
EcoO109I RGGNCCY 1 cut(s) 916
EcoO65I GGTNACC 2 cut(s) 649, 688
EcoRII CCWGG 5 cut(s) 17, 176, 243, 912, 1217
EcoT14I CCWWGG 3 cut(s) 314, 466, 613
EcoT22I ATGCAT 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 1237
ErhI CCWWGG 3 cut(s) 314, 466, 613
Esp3I CGTCTC 1 cut(s) 1133
FaeI CATG 7 cut(s) 92, 118, 318, 446, 626, 710, 1017
FaqI GGGAC 1 cut(s) 595
FatI CATG 7 cut(s) 88, 114, 314, 442, 622, 706, 1013
FauI CCCGC 2 cut(s) 831, 1088
FbaI TGATCA 1 cut(s) 1174
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 230, 365
FokI GGATG 4 cut(s) 260, 331, 515, 535
FriOI GRGCYC 1 cut(s) 1237
Fsp4HI GCNGC 2 cut(s) 230, 365
FspBI CTAG 5 cut(s) 233, 416, 453, 786, 837
GluI GCNGC 2 cut(s) 230, 365
GsaI CCCAGC 3 cut(s) 666, 1028, 1196
GsuI CTGGAG 2 cut(s) 71, 1044
HaeIII GGCC 7 cut(s) 201, 367, 471, 802, 835, 892, 936
HapII CCGG 3 cut(s) 293, 382, 591
Hin1II CATG 7 cut(s) 92, 118, 318, 446, 626, 710, 1017
HincII GTYRAC 2 cut(s) 184, 730
HindII GTYRAC 2 cut(s) 184, 730
HinfI GANTC 5 cut(s) 119, 425, 726, 1103, 1157
HpaII CCGG 3 cut(s) 293, 382, 591
HphI GGTGA 5 cut(s) 299, 593, 682, 901, 1126
Hpy166II GTNNAC 6 cut(s) 184, 379, 620, 717, 730, 1019
Hpy188I TCNGA 8 cut(s) 34, 481, 519, 526, 843, 1110, 1164, 1210
Hpy188III TCNNGA 3 cut(s) 50, 97, 123
Hpy8I GTNNAC 6 cut(s) 184, 379, 620, 717, 730, 1019
Hpy99I CGWCG 1 cut(s) 373
HpyAV CCTTC 5 cut(s) 100, 142, 191, 524, 984
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 5 cut(s) 80, 255, 437, 446, 761
HpyF10VI GCNNNNNNNGC 5 cut(s) 235, 355, 364, 443, 832
HpyF3I CTNAG 5 cut(s) 33, 98, 525, 1109, 1236
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 7 cut(s) 92, 118, 318, 446, 626, 710, 1017
Ksp22I TGATCA 1 cut(s) 1174
Kzo9I GATC 4 cut(s) 36, 519, 1174, 1253
LmnI GCTCC 3 cut(s) 976, 1033, 1057
LweI GCATC 2 cut(s) 67, 446
MaeI CTAG 5 cut(s) 233, 416, 453, 786, 837
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 7 cut(s) 206, 215, 505, 599, 634, 649, 688
MalI GATC 4 cut(s) 38, 521, 1176, 1255
MbiI CCGCTC 2 cut(s) 364, 1083
MboI GATC 4 cut(s) 36, 519, 1174, 1253
MboII GAAGA 9 cut(s) 71, 77, 334, 551, 948, 1236, 1254, 1260, 1263
MhlI GDGCHC 2 cut(s) 240, 1237
MluCI AATT 2 cut(s) 259, 556
MlyI GAGTC 2 cut(s) 735, 1097
MmeI TCCRAC 1 cut(s) 432
Mph1103I ATGCAT 1 cut(s) 439
MseI TTAA 3 cut(s) 560, 924, 963
MslI CAYNNNNRTG 2 cut(s) 246, 578
MspI CCGG 3 cut(s) 293, 382, 591
MspR9I CCNGG 6 cut(s) 19, 178, 245, 591, 914, 1219
Mva1269I GAATGC 1 cut(s) 19
MvaI CCWGG 5 cut(s) 19, 178, 245, 914, 1219
MwoI GCNNNNNNNGC 5 cut(s) 235, 355, 364, 443, 832
NciI CCSGG 1 cut(s) 591
NcoI CCATGG 1 cut(s) 314
NdeII GATC 4 cut(s) 36, 519, 1174, 1253
NlaIII CATG 7 cut(s) 92, 118, 318, 446, 626, 710, 1017
NlaIV GGNNCC 3 cut(s) 239, 918, 972
NmuCI GTSAC 4 cut(s) 206, 505, 599, 688
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 3 cut(s) 446, 626, 710
OliI CACNNNNGTG 1 cut(s) 578
PceI AGGCCT 2 cut(s) 201, 892
PciI ACATGT 1 cut(s) 706
PctI GAATGC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 119, 425, 1157
PkrI GCNGC 2 cut(s) 231, 366
PleI GAGTC 2 cut(s) 734, 1097
PpsI GAGTC 2 cut(s) 734, 1097
PpuMI RGGWCCY 1 cut(s) 916
PscI ACATGT 1 cut(s) 706
Psp124BI GAGCTC 1 cut(s) 1237
Psp1406I AACGTT 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 916
Psp6I CCWGG 5 cut(s) 17, 176, 243, 912, 1217
PspEI GGTNACC 2 cut(s) 649, 688
PspFI CCCAGC 3 cut(s) 662, 1024, 1192
PspGI CCWGG 5 cut(s) 17, 176, 243, 912, 1217
PspN4I GGNNCC 3 cut(s) 239, 918, 972
PspPI GGNCC 7 cut(s) 311, 405, 617, 717, 833, 916, 935
PspPPI RGGWCCY 1 cut(s) 916
RsaI GTAC 3 cut(s) 308, 790, 813
RsaNI GTAC 3 cut(s) 307, 789, 812
RseI CAYNNNNRTG 2 cut(s) 246, 578
SacI GAGCTC 1 cut(s) 1237
SalI GTCGAC 1 cut(s) 182
SaqAI TTAA 3 cut(s) 560, 924, 963
SatI GCNGC 2 cut(s) 230, 365
Sau3AI GATC 4 cut(s) 36, 519, 1174, 1253
Sau96I GGNCC 7 cut(s) 311, 405, 617, 717, 833, 916, 935
SchI GAGTC 2 cut(s) 735, 1097
ScrFI CCNGG 6 cut(s) 19, 178, 245, 591, 914, 1219
SduI GDGCHC 2 cut(s) 240, 1237
SfaNI GCATC 2 cut(s) 67, 446
SfcI CTRYAG 2 cut(s) 796, 1065
SinI GGWCC 5 cut(s) 311, 405, 617, 717, 916
SmiI ATTTAAAT 1 cut(s) 561
SmiMI CAYNNNNRTG 2 cut(s) 246, 578
SmlI CTYRAG 2 cut(s) 278, 359
SmoI CTYRAG 2 cut(s) 278, 359
SpeI ACTAGT 1 cut(s) 785
Sse9I AATT 2 cut(s) 259, 556
SseBI AGGCCT 2 cut(s) 201, 892
SsiI CCGC 5 cut(s) 229, 364, 686, 824, 1081
SspI AATATT 1 cut(s) 301
SspMI CTAG 5 cut(s) 233, 416, 453, 786, 837
SstI GAGCTC 1 cut(s) 1237
StuI AGGCCT 2 cut(s) 201, 892
StyD4I CCNGG 6 cut(s) 17, 176, 243, 589, 912, 1217
StyI CCWWGG 3 cut(s) 314, 466, 613
SwaI ATTTAAAT 1 cut(s) 561
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 1 cut(s) 491
TaqI TCGA 3 cut(s) 183, 1231, 1256
TasI AATT 2 cut(s) 259, 556
TauI GCSGC 2 cut(s) 232, 367
TfiI GAWTC 3 cut(s) 119, 425, 1157
Tru1I TTAA 3 cut(s) 560, 924, 963
Tru9I TTAA 3 cut(s) 560, 924, 963
TscAI CASTG 2 cut(s) 877, 1268
TseFI GTSAC 4 cut(s) 206, 505, 599, 688
Tsp45I GTSAC 4 cut(s) 206, 505, 599, 688
TspDTI ATGAA 6 cut(s) 17, 77, 335, 552, 948, 1002
TspRI CASTG 2 cut(s) 877, 1268
VpaK11BI GGWCC 5 cut(s) 311, 405, 617, 717, 916
XagI CCTNNNNNAGG 2 cut(s) 175, 281
XapI RAATTY 1 cut(s) 259
XceI RCATGY 3 cut(s) 446, 626, 710
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 5 cut(s) 233, 416, 453, 786, 837
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.