Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005740

Physical Location & Seq

Genomic Coordinates Forward (+)

590879 .. 595603

Gene Structure 4725 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1004200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 960 bp

ATGGCTACCGCCGCCGCTCAGTCTGCTACTCAGAGTTTTTCAGGTGGAGATGATGCCAGTGTTTTTCAGTTGATCCAATCTCATCAGGAAAAGGCTGCTCGGCTTCCCCCGGTTGAGGAAATCCGTACTCTACTTGATCGTAGTGTTCGCGGAACGCTCTCCACTTTTTCTCAGAAGCATGAGGATCATCCATCAGGATCAATGGTTGACTTTGCATGTGATGCAGATGGATCTCCAATATTAGCAATTAGCAGCTTGGCAGTTCATACAAAGGACCTAGCCATTAATCCCAAGTGCTCATTGCTTGTGGCTAAAGACCCTGAAGATAGGACTGATTTGGTGGTCACGCTGCATGGTGATGCTATTCCTGTTTCCGAGATGGATCAAGCAGCCATACGCACTGCATATTTGGCTAAGCATCCCAATGCATTTTCGGTTGACTTTGGCGACTTCCAATTCATGCGCATTGAACCAAAAGTTGTGCGATACGTGTCAGGGGTTGCAACAGCCTTGTTAGGATCAGGAGAGTTCGGAAGTGAGGAGTATAAAGCTGCAAAAGTTGATCCAATTGCTCAATTTTCAAAGCCTGTTGCAAATCTTAAAACTATTGCTTCCCAGTCTCACATGAACAGGGATCATGCTGACGATACAAAAGCCATTGTGCAACACTCGACGTCTATTCCGGTGGACTCTGCTTACATGTTGGACTTGGACAGCCTGGGTTTCAATGTTAAGGTTGGCTATCAGGGAGATAATTTCAAGCTCCGCATACCTTTTCCTAGACGTGCAGAGGATCGAAAATTGCTATCAAAAGGATGTGAAGACTTTAGTAGTGGAAATGCTTCAGGCTGCAAGGGCTCACGATCGTTAATCTCACCGTACGTAACAAGGATATTTCAGCACCTCGGAACTGCCATCACAGAGATATGGAAAAATTTGGACTCAAAAGAGGTGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF2470 Putative_PNPOx Pyrid_oxidase_2

Protein Analysis

320

Amino Acids

34.64

Weight (kDa)

6.06

Isoelectric Point (pI)

36.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PNPOx_N	PF01243	39 - 164	2.8e-12	Pyridoxamine 5'-phosphate oxidase
CREG_beta-barrel	PF13883	42 - 165	8.2e-12	CREG, beta-barrel
DUF2470	PF10615	207 - 267	9.9e-15	Domain of unknown function (DUF2470)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03890 AT3G03890
fragaria_vesca	FvH4_1g28200 FvH4_1g28200 FvH4_1g28200 FvH4_1g28200
malus_domestica	MD12G1004000.v1.1 MD12G1004200.v1.1 MD14G1002900.v1.1
prunus_persica	Prupe.7G004600_v2.0.a1 Prupe.7G004600_v2.0.a1 Prupe.7G004600_v2.0.a1
pyrus_communis	pycom12g00350 pycom14g00080
rosa_chinensis	RchiOBHm_Chr3g0497041
rosa_laevigata	RLG00000022486
rosa_multiflora	Rmu_sc0008737.1_g000002 Rmu_sc0009312.1_g000002 Rmu_sc0035985.1_g000001 Rmu_sc0036941.1_g000001
rosa_roxburghii	Rroxscaffold_6G00388440
rosa_rugosa	Rorug03G0289000 Rorug03G0289100
rosa_samantha	Rh3AG333400 Rh3BG370700 Rh3CG367400 Rh3DG368700
rosa_wichuraiana	Rw3G029290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	677
Acc16I	TGCGCA	1 cut(s)	464
AccBSI	CCGCTC	1 cut(s)	17
AccII	CGCG	1 cut(s)	150
AciI	CCGC	5 cut(s)	9, 12, 15, 150, 766
AclWI	GGATC	9 cut(s)	67, 192, 205, 238, 390, 526, 557, 642, 801
AcsI	RAATTY	1 cut(s)	934
AcuI	CTGAAG	2 cut(s)	342, 828
AcyI	GRCGYC	1 cut(s)	674
AfaI	GTAC	2 cut(s)	127, 881
AfiI	CCNNNNNNNGG	1 cut(s)	115
AflIII	ACRYGT	2 cut(s)	489, 699
AgsI	TTSAA	4 cut(s)	470, 582, 727, 760
AjiI	CACGTC	1 cut(s)	785
AjnI	CCWGG	1 cut(s)	717
AluBI	AGCT	3 cut(s)	255, 551, 763
AluI	AGCT	3 cut(s)	255, 551, 763
Alw21I	GWGCWC	1 cut(s)	299
Alw26I	GTCTC	1 cut(s)	624
AlwI	GGATC	9 cut(s)	67, 192, 205, 238, 390, 526, 557, 642, 801
ApeKI	GCWGC	6 cut(s)	95, 252, 349, 389, 551, 849
ApoI	RAATTY	1 cut(s)	934
AseI	ATTAAT	1 cut(s)	285
Asp700I	GAANNNNTTC	1 cut(s)	841
AspLEI	GCGC	1 cut(s)	465
AspS9I	GGNCC	1 cut(s)	274
AsuC2I	CCSGG	1 cut(s)	110
AsuHPI	GGTGA	2 cut(s)	368, 867
AvaII	GGWCC	1 cut(s)	274
BanII	GRGCYC	1 cut(s)	860
BbsI	GAAGAC	1 cut(s)	828
Bbv12I	GWGCWC	1 cut(s)	299
BbvI	GCAGC	6 cut(s)	82, 264, 336, 401, 538, 836
BccI	CCATC	4 cut(s)	199, 221, 373, 923
BciT130I	CCWGG	1 cut(s)	719
BcnI	CCSGG	1 cut(s)	110
BcoDI	GTCTC	1 cut(s)	624
BfaI	CTAG	2 cut(s)	278, 780
BisI	GCNGC	8 cut(s)	12, 15, 96, 253, 350, 390, 552, 850
BlpI	GCTNAGC	1 cut(s)	414
BlsI	GCNGC	8 cut(s)	13, 16, 97, 254, 351, 391, 553, 851
Bme1390I	CCNGG	2 cut(s)	110, 719
Bme18I	GGWCC	1 cut(s)	274
BmgBI	CACGTC	1 cut(s)	785
BmgT120I	GGNCC	1 cut(s)	274
BmrFI	CCNGG	2 cut(s)	110, 719
BmrI	ACTGGG	1 cut(s)	610
BmsI	GCATC	4 cut(s)	43, 211, 349, 427
BmuI	ACTGGG	1 cut(s)	610
BpiI	GAAGAC	1 cut(s)	828
Bpu1102I	GCTNAGC	1 cut(s)	414
BpuMI	CCSGG	1 cut(s)	110
BsaAI	YACGTR	2 cut(s)	490, 883
BsaHI	GRCGYC	1 cut(s)	674
BsaJI	CCNNGG	3 cut(s)	108, 718, 904
BsaWI	WCCGGW	1 cut(s)	682
Bsc4I	CCNNNNNNNGG	1 cut(s)	115
Bse1I	ACTGG	2 cut(s)	57, 616
Bse3DI	GCAATG	1 cut(s)	299
BseBI	CCWGG	1 cut(s)	719
BseDI	CCNNGG	3 cut(s)	108, 718, 904
BseGI	GGATG	3 cut(s)	187, 418, 821
BseLI	CCNNNNNNNGG	1 cut(s)	115
BseMI	GCAATG	1 cut(s)	299
BseMII	CTCAG	3 cut(s)	32, 44, 185
BseNI	ACTGG	2 cut(s)	57, 616
BseRI	GAGGAG	1 cut(s)	554
BseXI	GCAGC	6 cut(s)	82, 264, 336, 401, 538, 836
BsgI	GTGCAG	1 cut(s)	807
Bsh1236I	CGCG	1 cut(s)	150
Bsh1285I	CGRYCG	1 cut(s)	866
BsiEI	CGRYCG	1 cut(s)	866
BsiHKAI	GWGCWC	1 cut(s)	299
BsiSI	CCGG	2 cut(s)	110, 683
BsiWI	CGTACG	1 cut(s)	879
BslI	CCNNNNNNNGG	1 cut(s)	115
BsmAI	GTCTC	1 cut(s)	624
Bsp1286I	GDGCHC	2 cut(s)	299, 860
Bsp1720I	GCTNAGC	1 cut(s)	414
BspACI	CCGC	5 cut(s)	9, 12, 15, 150, 766
BspCNI	CTCAG	3 cut(s)	31, 43, 184
BspFNI	CGCG	1 cut(s)	150
BspPI	GGATC	9 cut(s)	67, 192, 205, 238, 390, 526, 557, 642, 801
BsrBI	CCGCTC	1 cut(s)	17
BsrDI	GCAATG	1 cut(s)	299
BsrI	ACTGG	2 cut(s)	57, 616
BssECI	CCNNGG	3 cut(s)	108, 718, 904
BssNI	GRCGYC	1 cut(s)	674
Bst2UI	CCWGG	1 cut(s)	719
Bst4CI	ACNGT	1 cut(s)	879
BstACI	GRCGYC	1 cut(s)	674
BstAPI	GCANNNNNTGC	1 cut(s)	221
BstBAI	YACGTR	2 cut(s)	490, 883
BstDEI	CTNAG	4 cut(s)	18, 30, 171, 414
BstF5I	GGATG	3 cut(s)	187, 418, 821
BstFNI	CGCG	1 cut(s)	150
BstHHI	GCGC	1 cut(s)	465
BstMAI	GTCTC	1 cut(s)	624
BstMCI	CGRYCG	1 cut(s)	866
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 23, 221, 410, 855
BstNI	CCWGG	1 cut(s)	719
BstNSI	RCATGY	2 cut(s)	219, 703
BstSCI	CCNGG	2 cut(s)	108, 717
BstSNI	TACGTA	1 cut(s)	883
BstUI	CGCG	1 cut(s)	150
BstV1I	GCAGC	6 cut(s)	82, 264, 336, 401, 538, 836
BstV2I	GAAGAC	1 cut(s)	828
BstX2I	RGATCY	1 cut(s)	230
BstYI	RGATCY	1 cut(s)	230
BtrI	CACGTC	1 cut(s)	785
BtsCI	GGATG	3 cut(s)	187, 418, 821
BtsI	GCAGTG	1 cut(s)	399
BtsIMutI	CAGTG	2 cut(s)	64, 399
CfoI	GCGC	1 cut(s)	465
Cfr13I	GGNCC	1 cut(s)	274
Csp6I	GTAC	2 cut(s)	126, 880
CviAII	CATG	7 cut(s)	179, 216, 353, 460, 625, 638, 700
CviQI	GTAC	2 cut(s)	126, 880
DdeI	CTNAG	4 cut(s)	18, 30, 171, 414
Eco105I	TACGTA	1 cut(s)	883
Eco24I	GRGCYC	1 cut(s)	860
Eco47I	GGWCC	1 cut(s)	274
Eco57I	CTGAAG	2 cut(s)	342, 828
EcoO109I	RGGNCCY	1 cut(s)	274
EcoRII	CCWGG	1 cut(s)	717
EcoT22I	ATGCAT	1 cut(s)	430
EcoT38I	GRGCYC	1 cut(s)	860
FaeI	CATG	7 cut(s)	182, 219, 356, 463, 628, 641, 703
FatI	CATG	7 cut(s)	178, 215, 352, 459, 624, 637, 699
Fnu4HI	GCNGC	8 cut(s)	12, 15, 96, 253, 350, 390, 552, 850
FokI	GGATG	3 cut(s)	174, 405, 828
FriOI	GRGCYC	1 cut(s)	860
Fsp4HI	GCNGC	8 cut(s)	12, 15, 96, 253, 350, 390, 552, 850
FspAI	RTGCGCAY	1 cut(s)	464
FspBI	CTAG	2 cut(s)	278, 780
FspI	TGCGCA	1 cut(s)	464
GlaI	GCGC	1 cut(s)	464
GluI	GCNGC	8 cut(s)	12, 15, 96, 253, 350, 390, 552, 850
HapII	CCGG	2 cut(s)	110, 683
HhaI	GCGC	1 cut(s)	465
Hin1I	GRCGYC	1 cut(s)	674
Hin1II	CATG	7 cut(s)	182, 219, 356, 463, 628, 641, 703
Hin6I	GCGC	1 cut(s)	463
HinP1I	GCGC	1 cut(s)	463
HincII	GTYRAC	2 cut(s)	208, 439
HindII	GTYRAC	2 cut(s)	208, 439
HinfI	GANTC	2 cut(s)	689, 941
HpaII	CCGG	2 cut(s)	110, 683
HphI	GGTGA	2 cut(s)	368, 867
Hpy166II	GTNNAC	3 cut(s)	208, 439, 688
Hpy188I	TCNGA	5 cut(s)	33, 174, 376, 533, 908
Hpy188III	TCNNGA	4 cut(s)	86, 195, 522, 861
Hpy8I	GTNNAC	3 cut(s)	208, 439, 688
Hpy99I	CGWCG	1 cut(s)	676
HpyCH4III	ACNGT	1 cut(s)	879
HpyCH4IV	ACGT	4 cut(s)	489, 674, 784, 882
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 23, 221, 410, 855
HpyF3I	CTNAG	4 cut(s)	18, 30, 171, 414
HpySE526I	ACGT	4 cut(s)	489, 674, 784, 882
Hsp92I	GRCGYC	1 cut(s)	674
Hsp92II	CATG	7 cut(s)	182, 219, 356, 463, 628, 641, 703
HspAI	GCGC	1 cut(s)	463
LmnI	GCTCC	1 cut(s)	768
Lsp1109I	GCAGC	6 cut(s)	82, 264, 336, 401, 538, 836
LweI	GCATC	4 cut(s)	43, 211, 349, 427
MaeI	CTAG	2 cut(s)	278, 780
MaeII	ACGT	4 cut(s)	489, 674, 784, 882
MaeIII	GTNAC	2 cut(s)	343, 883
MbiI	CCGCTC	1 cut(s)	17
MboII	GAAGA	2 cut(s)	335, 833
MfeI	CAATTG	1 cut(s)	567
MflI	RGATCY	1 cut(s)	230
MhlI	GDGCHC	2 cut(s)	299, 860
MluCI	AATT	7 cut(s)	246, 455, 567, 575, 754, 800, 934
MlyI	GAGTC	2 cut(s)	683, 935
MmeI	TCCRAC	1 cut(s)	684
MnlI	CCTC	6 cut(s)	109, 175, 532, 784, 914, 943
Mph1103I	ATGCAT	1 cut(s)	430
MroXI	GAANNNNTTC	1 cut(s)	841
MseI	TTAA	4 cut(s)	285, 600, 732, 869
MslI	CAYNNNNRTG	2 cut(s)	357, 423
MspI	CCGG	2 cut(s)	110, 683
MspR9I	CCNGG	2 cut(s)	110, 719
MunI	CAATTG	1 cut(s)	567
MvaI	CCWGG	1 cut(s)	719
MvnI	CGCG	1 cut(s)	150
MwoI	GCNNNNNNNGC	5 cut(s)	11, 23, 221, 410, 855
NciI	CCSGG	1 cut(s)	110
NlaIII	CATG	7 cut(s)	182, 219, 356, 463, 628, 641, 703
NmeAIII	GCCGAG	1 cut(s)	79
NmuCI	GTSAC	1 cut(s)	343
NsbI	TGCGCA	1 cut(s)	464
NsiI	ATGCAT	1 cut(s)	430
NspI	RCATGY	2 cut(s)	219, 703
PciI	ACATGT	1 cut(s)	699
PdmI	GAANNNNTTC	1 cut(s)	841
Pfl23II	CGTACG	1 cut(s)	879
PkrI	GCNGC	8 cut(s)	13, 16, 97, 254, 351, 391, 553, 851
Ple19I	CGATCG	1 cut(s)	866
PleI	GAGTC	2 cut(s)	683, 935
PpsI	GAGTC	2 cut(s)	683, 935
Ppu21I	YACGTR	2 cut(s)	490, 883
PpuMI	RGGWCCY	1 cut(s)	274
PscI	ACATGT	1 cut(s)	699
PshBI	ATTAAT	1 cut(s)	285
Psp5II	RGGWCCY	1 cut(s)	274
Psp6I	CCWGG	1 cut(s)	717
PspGI	CCWGG	1 cut(s)	717
PspLI	CGTACG	1 cut(s)	879
PspPI	GGNCC	1 cut(s)	274
PspPPI	RGGWCCY	1 cut(s)	274
PsuI	RGATCY	1 cut(s)	230
PvuI	CGATCG	1 cut(s)	866
RsaI	GTAC	2 cut(s)	127, 881
RsaNI	GTAC	2 cut(s)	126, 880
RseI	CAYNNNNRTG	2 cut(s)	357, 423
SaqAI	TTAA	4 cut(s)	285, 600, 732, 869
SatI	GCNGC	8 cut(s)	12, 15, 96, 253, 350, 390, 552, 850
Sau96I	GGNCC	1 cut(s)	274
SchI	GAGTC	2 cut(s)	683, 935
ScrFI	CCNGG	2 cut(s)	110, 719
SduI	GDGCHC	2 cut(s)	299, 860
SfaNI	GCATC	4 cut(s)	43, 211, 349, 427
SinI	GGWCC	1 cut(s)	274
SmiMI	CAYNNNNRTG	2 cut(s)	357, 423
SnaBI	TACGTA	1 cut(s)	883
Sse9I	AATT	7 cut(s)	246, 455, 567, 575, 754, 800, 934
SsiI	CCGC	5 cut(s)	9, 12, 15, 150, 766
SspI	AATATT	1 cut(s)	240
SspMI	CTAG	2 cut(s)	278, 780
StyD4I	CCNGG	2 cut(s)	108, 717
TaaI	ACNGT	1 cut(s)	879
TaiI	ACGT	4 cut(s)	492, 677, 787, 885
TaqI	TCGA	2 cut(s)	671, 796
TasI	AATT	7 cut(s)	246, 455, 567, 575, 754, 800, 934
TauI	GCSGC	2 cut(s)	14, 17
Tru1I	TTAA	4 cut(s)	285, 600, 732, 869
Tru9I	TTAA	4 cut(s)	285, 600, 732, 869
TscAI	CASTG	2 cut(s)	64, 406
TseFI	GTSAC	1 cut(s)	343
TseI	GCWGC	6 cut(s)	95, 252, 349, 389, 551, 849
Tsp45I	GTSAC	1 cut(s)	343
TspDTI	ATGAA	3 cut(s)	254, 448, 641
TspGWI	ACGGA	1 cut(s)	113
TspRI	CASTG	2 cut(s)	64, 406
VpaK11BI	GGWCC	1 cut(s)	274
VspI	ATTAAT	1 cut(s)	285
XapI	RAATTY	1 cut(s)	934
XceI	RCATGY	2 cut(s)	219, 703
XmnI	GAANNNNTTC	1 cut(s)	841
XspI	CTAG	2 cut(s)	278, 780
ZraI	GACGTC	1 cut(s)	675
Zsp2I	ATGCAT	1 cut(s)	430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.