MD12G1008400.v1.1

post-GPI attachment to proteins factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007052
Physical Location & Seq
Forward (+)
915357 .. 919630
4274 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1008400.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGATGATGTTATTGATGTTAAAGCATTATTGGGTTGCTTTCTTTGTGGTGGTGTCATGGGCTGTCAGCTTCACAGTTTTAGATGCCAGTGCTGGTGATGCTGATCCGCGCTATAGGGCTTGTCTAAACGAATGTGGAGAAACCGGTTGTGTAGGGCAGAGATGCTTTCCACATTGCAATTCCTCTTCCGATGGGGTCTCTGTTGATGGTCCATGGTACATGCAAGAACCTCTTTATCAGCAGTGGAAACAATGGGATTGCCAAAGTGATTGCCGTTACTATTGTATGGTTGACAGAGAGAAAGAAAGAGAAGCAGCTGGTGATGGTCCAGTCAAATATCATGGTAAATGGCCCTTCAAGCGTATTTACGGGATCCAGGAGCCTGCTTCCGTAGCTTTCTCTGTGCTCAACCTTGCAACGCATTTTCATGGTTGGATATCCTTTTTCATCCTTTTATACTACAACTTGCCTCTGAGACGGGATAAGAAGACGTACTATTATTTTGCTAGTTTATGGCATATCTATGCTCTCTTGTCATTGACCTCCTGGTTCGCGAGTGCTGTGTTCCATAGCAAGGACGTGGATTTGACCGAGAAATTAGACTACTCATGTGCAGTGGCAATACTTGGGTACTCGCTTATTCTGTCCATACTAAGAAGTTTTGATGTGAAAGATGATGCCGTCAGAGTGATGGTTGCTGCTCCACTGCTTGCTTTCGTAACCACCCACATATTGTACCTCAACTTCTATAAACTGGATTACGGGTGGAACATGCAGGTTTGTGTTGTCATGGCGGTGGTTCAACTTCTTCTTTGGGCAGTTTGGGCTGGCGTCACTCGCCATCCATCACGTTGGAAGCTGTGGGTGGTAGTAGTGGGAGGAGGCCTTGCAATGCTCCTAGAAATCTATGACTTCCCTCCATATTACGGATTCTTGGATGCTCATGCCGCCTGGCATGCCACCACGATTCCTCTAACTTATATTTGGTGGAGTTTTATCCGAGACGATGCTGAGTTCGTGGCATCTAATCAAGTAAAGAGGTTGCGAAACTCGAACGTTGCCAAGAAGGCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Per1

Protein Analysis

359

Amino Acids

41.34

Weight (kDa)

7.97

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Per1 PF04080 77 - 337 1.8e-96 Per1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 766
AccII CGCG 2 cut(s) 109, 554
AciI CCGC 3 cut(s) 107, 794, 948
AclI AACGTT 1 cut(s) 1056
AclWI GGATC 3 cut(s) 98, 367, 380
AcyI GRCGYC 1 cut(s) 831
AfaI GTAC 4 cut(s) 218, 494, 632, 737
AfiI CCNNNNNNNGG 2 cut(s) 574, 926
AgeI ACCGGT 1 cut(s) 143
AgsI TTSAA 2 cut(s) 358, 803
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 3 cut(s) 375, 545, 950
AluBI AGCT 4 cut(s) 69, 317, 395, 859
AluI AGCT 4 cut(s) 69, 317, 395, 859
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 3 cut(s) 202, 469, 996
AlwI GGATC 3 cut(s) 98, 367, 380
AoxI GGCC 2 cut(s) 350, 883
ApeKI GCWGC 2 cut(s) 314, 698
AsiGI ACCGGT 1 cut(s) 143
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 3 cut(s) 209, 326, 351
AsuHPI GGTGA 2 cut(s) 107, 332
AvaII GGWCC 2 cut(s) 209, 326
BamHI GGATCC 1 cut(s) 372
BarI GAAGNNNNNNTAC 2 cut(s) 728, 760
BbsI GAAGAC 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 326, 685
BccI CCATC 6 cut(s) 185, 200, 317, 685, 849, 853
BceAI ACGGC 2 cut(s) 258, 665
BciT130I CCWGG 3 cut(s) 377, 547, 952
BcoDI GTCTC 3 cut(s) 202, 469, 996
BfaI CTAG 2 cut(s) 507, 899
BfmI CTRYAG 1 cut(s) 112
BfuAI ACCTGC 1 cut(s) 766
BglI GCCNNNNNGGC 1 cut(s) 1067
BisI GCNGC 3 cut(s) 315, 699, 948
BlsI GCNGC 3 cut(s) 316, 700, 949
Bme1390I CCNGG 3 cut(s) 377, 547, 952
Bme18I GGWCC 2 cut(s) 209, 326
BmgBI CACGTC 1 cut(s) 580
BmgT120I GGNCC 3 cut(s) 209, 326, 351
BmiI GGNNCC 2 cut(s) 374, 381
BmrFI CCNGG 3 cut(s) 377, 547, 952
BmsI GCATC 7 cut(s) 73, 88, 152, 667, 928, 997, 1031
BpiI GAAGAC 1 cut(s) 494
BsaBI GATNNNNATC 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 212
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 2 cut(s) 574, 926
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 3 cut(s) 87, 329, 759
Bse3DI GCAATG 2 cut(s) 172, 897
Bse8I GATNNNNATC 1 cut(s) 102
BseBI CCWGG 3 cut(s) 377, 547, 952
BseDI CCNNGG 1 cut(s) 212
BseGI GGATG 3 cut(s) 447, 841, 943
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 574, 926
BseMI GCAATG 2 cut(s) 172, 897
BseMII CTCAG 2 cut(s) 464, 1002
BseNI ACTGG 3 cut(s) 87, 329, 759
BseRI GAGGAG 1 cut(s) 894
BseXI GCAGC 2 cut(s) 326, 685
BsgI GTGCAG 1 cut(s) 633
Bsh1236I CGCG 2 cut(s) 109, 554
BshFI GGCC 2 cut(s) 352, 885
BshTI ACCGGT 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 1 cut(s) 144
BslI CCNNNNNNNGG 2 cut(s) 574, 926
BsmAI GTCTC 3 cut(s) 202, 469, 996
BsmBI CGTCTC 2 cut(s) 469, 996
BsnI GGCC 2 cut(s) 352, 885
Bso31I GGTCTC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 2 cut(s) 103, 372
Bsp19I CCATGG 1 cut(s) 212
Bsp68I TCGCGA 1 cut(s) 554
BspACI CCGC 3 cut(s) 107, 794, 948
BspANI GGCC 2 cut(s) 352, 885
BspCNI CTCAG 2 cut(s) 465, 1003
BspFNI CGCG 2 cut(s) 109, 554
BspLI GGNNCC 2 cut(s) 374, 381
BspMI ACCTGC 1 cut(s) 766
BspPI GGATC 3 cut(s) 98, 367, 380
BspTNI GGTCTC 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 172, 897
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 3 cut(s) 87, 329, 759
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 2 cut(s) 103, 372
BssNI GRCGYC 1 cut(s) 831
BssT1I CCWWGG 1 cut(s) 212
Bst2UI CCWGG 3 cut(s) 377, 547, 952
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 190
BstACI GRCGYC 1 cut(s) 831
BstC8I GCNNGC 4 cut(s) 384, 711, 829, 957
BstDEI CTNAG 3 cut(s) 473, 653, 1011
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 3 cut(s) 447, 841, 943
BstFNI CGCG 2 cut(s) 109, 554
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 2 cut(s) 106, 375
BstMAI GTCTC 3 cut(s) 202, 469, 996
BstMBI GATC 2 cut(s) 103, 372
BstMWI GCNNNNNNNGC 8 cut(s) 98, 358, 392, 824, 837, 947, 956, 1067
BstNI CCWGG 3 cut(s) 377, 547, 952
BstNSI RCATGY 3 cut(s) 223, 775, 959
BstSCI CCNGG 3 cut(s) 375, 545, 950
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 2 cut(s) 109, 554
BstV1I GCAGC 2 cut(s) 326, 685
BstV2I GAAGAC 1 cut(s) 494
BstX2I RGATCY 1 cut(s) 372
BstXI CCANNNNNNTGG 1 cut(s) 852
BstYI RGATCY 1 cut(s) 372
BsuRI GGCC 2 cut(s) 352, 885
BtgI CCRYGG 1 cut(s) 212
BtrI CACGTC 1 cut(s) 580
BtsCI GGATG 3 cut(s) 447, 841, 943
BtsI GCAGTG 3 cut(s) 248, 621, 704
BtsIMutI CAGTG 4 cut(s) 94, 248, 621, 704
BtuMI TCGCGA 1 cut(s) 554
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 4 cut(s) 384, 711, 829, 957
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 3 cut(s) 209, 326, 351
CseI GACGC 1 cut(s) 820
Csp6I GTAC 4 cut(s) 217, 493, 631, 736
CspAI ACCGGT 1 cut(s) 143
CviQI GTAC 4 cut(s) 217, 493, 631, 736
DdeI CTNAG 3 cut(s) 473, 653, 1011
DpnI GATC 2 cut(s) 105, 374
DpnII GATC 2 cut(s) 103, 372
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 212
Eco147I AGGCCT 1 cut(s) 885
Eco31I GGTCTC 1 cut(s) 202
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 209, 326
EcoRII CCWGG 3 cut(s) 375, 545, 950
EcoRV GATATC 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 212
Esp3I CGTCTC 2 cut(s) 469, 996
FalI AAGNNNNNCTT 2 cut(s) 216, 248
Fnu4HI GCNGC 3 cut(s) 315, 699, 948
FokI GGATG 3 cut(s) 434, 828, 950
Fsp4HI GCNGC 3 cut(s) 315, 699, 948
FspBI CTAG 2 cut(s) 507, 899
GlaI GCGC 1 cut(s) 110
GluI GCNGC 3 cut(s) 315, 699, 948
HaeIII GGCC 2 cut(s) 352, 885
HapII CCGG 1 cut(s) 144
HgaI GACGC 1 cut(s) 820
HhaI GCGC 1 cut(s) 111
Hin1I GRCGYC 1 cut(s) 831
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 930, 967
HpaII CCGG 1 cut(s) 144
HphI GGTGA 2 cut(s) 107, 332
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 4 cut(s) 190, 474, 686, 1001
Hpy188III TCNNGA 1 cut(s) 553
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 2 cut(s) 364, 1060
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 4 cut(s) 491, 579, 850, 1056
HpyCH4V TGCA 6 cut(s) 177, 223, 416, 614, 775, 890
HpyF10VI GCNNNNNNNGC 8 cut(s) 98, 358, 392, 824, 837, 947, 956, 1067
HpyF3I CTNAG 3 cut(s) 473, 653, 1011
HpySE526I ACGT 4 cut(s) 491, 579, 850, 1056
Hsp92I GRCGYC 1 cut(s) 831
HspAI GCGC 1 cut(s) 109
Kzo9I GATC 2 cut(s) 103, 372
LmnI GCTCC 3 cut(s) 379, 706, 900
Lsp1109I GCAGC 2 cut(s) 326, 685
LweI GCATC 7 cut(s) 73, 88, 152, 667, 928, 997, 1031
MaeI CTAG 2 cut(s) 507, 899
MaeII ACGT 4 cut(s) 491, 579, 850, 1056
MaeIII GTNAC 3 cut(s) 275, 718, 832
MalI GATC 2 cut(s) 105, 374
MboI GATC 2 cut(s) 103, 372
MboII GAAGA 3 cut(s) 177, 499, 800
MflI RGATCY 1 cut(s) 372
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 2 cut(s) 178, 596
MmeI TCCRAC 2 cut(s) 413, 833
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 3 cut(s) 426, 522, 794
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 3 cut(s) 377, 547, 952
MvaI CCWGG 3 cut(s) 377, 547, 952
MvnI CGCG 2 cut(s) 109, 554
MwoI GCNNNNNNNGC 8 cut(s) 98, 358, 392, 824, 837, 947, 956, 1067
NcoI CCATGG 1 cut(s) 212
NdeII GATC 2 cut(s) 103, 372
NlaIV GGNNCC 2 cut(s) 374, 381
NmuCI GTSAC 1 cut(s) 832
NruI TCGCGA 1 cut(s) 554
NspI RCATGY 3 cut(s) 223, 775, 959
PaeI GCATGC 1 cut(s) 959
PceI AGGCCT 1 cut(s) 885
PfeI GAWTC 2 cut(s) 930, 967
PfoI TCCNGGA 1 cut(s) 375
PinAI ACCGGT 1 cut(s) 143
PkrI GCNGC 3 cut(s) 316, 700, 949
Psp1406I AACGTT 1 cut(s) 1056
Psp6I CCWGG 3 cut(s) 375, 545, 950
PspGI CCWGG 3 cut(s) 375, 545, 950
PspN4I GGNNCC 2 cut(s) 374, 381
PspPI GGNCC 3 cut(s) 209, 326, 351
PsuI RGATCY 1 cut(s) 372
PvuII CAGCTG 1 cut(s) 317
RruI TCGCGA 1 cut(s) 554
RsaI GTAC 4 cut(s) 218, 494, 632, 737
RsaNI GTAC 4 cut(s) 217, 493, 631, 736
RseI CAYNNNNRTG 3 cut(s) 426, 522, 794
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 3 cut(s) 315, 699, 948
Sau3AI GATC 2 cut(s) 103, 372
Sau96I GGNCC 3 cut(s) 209, 326, 351
ScrFI CCNGG 3 cut(s) 377, 547, 952
SduI GDGCHC 1 cut(s) 408
SfaNI GCATC 7 cut(s) 73, 88, 152, 667, 928, 997, 1031
SfcI CTRYAG 1 cut(s) 112
SinI GGWCC 2 cut(s) 209, 326
SmiMI CAYNNNNRTG 3 cut(s) 426, 522, 794
SphI GCATGC 1 cut(s) 959
Sse9I AATT 2 cut(s) 178, 596
SseBI AGGCCT 1 cut(s) 885
SsiI CCGC 3 cut(s) 107, 794, 948
SspMI CTAG 2 cut(s) 507, 899
StuI AGGCCT 1 cut(s) 885
StyD4I CCNGG 3 cut(s) 375, 545, 950
StyI CCWWGG 1 cut(s) 212
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 4 cut(s) 494, 582, 853, 1059
TaqI TCGA 1 cut(s) 1052
TaqII GACCGA 1 cut(s) 605
TasI AATT 2 cut(s) 178, 596
TauI GCSGC 1 cut(s) 950
TfiI GAWTC 2 cut(s) 930, 967
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 4 cut(s) 94, 248, 621, 711
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 2 cut(s) 314, 698
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 2 cut(s) 416, 436
TspGWI ACGGA 2 cut(s) 379, 942
TspRI CASTG 4 cut(s) 94, 248, 621, 711
VpaK11BI GGWCC 2 cut(s) 209, 326
XceI RCATGY 3 cut(s) 223, 775, 959
XspI CTAG 2 cut(s) 507, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.