Rroxscaffold_1G00053070

post-GPI attachment to proteins factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007052
Physical Location & Seq
Reverse (-)
73888949 .. 73894013
5065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053070.1

Sequence Viewer

Length: 1266 bp
ATGTTGGTTTCAATATGGGTTGAACGAGTTCAGGCCCAGACTAATTTAACGTCCGAGCCCAAACTAGCTTTACATTCTAGACCCGTTTTCCGGCCCAAACCCGAAATCAATCTGAACTTAGAAGCAGCTGTTGAGCTTTTTGAATGGGTCAGACTGTCACTAGTGCCAAGGAAGAAGAAGAAGAAGAAGAAAGAGAGAGACGGAGGTTCCGGTGCTCCGATGTTAAAGCAGTACTGGATTGCTTTCTTTGTGGTGGTTTTATGGGGTTTTGGATTCAGAGCTATAGATGCCAGTGCTGGTGATGCTGATCCTCATTATAGGGCTTGTTTACAACAATGTGAAGAAACTGGATGTGTAGCACAGAGATGCTTTCCACAGTGCAATTTCTCTTCAGGTGGGGTCTCCGTTGATGGGCCATGGTTCCTGCAAGAACCTCTTTATTTGCAGTGGAAACAATGGGACTGCCAGAGTGATTGCCGATACTATTGTATGGTTGACAGAGAGAAAGAAAGAGAAGCAGCTGGTTATGGTCCGGTCAAATATCATGGTAAATGGCCCTTCAAGCGCGTTTATGGAATCCAGGAGCCTGCTTCTGTAGCTTTCTCTGCGCTCAACCTTGCGATGCATTTTCATGGTTGGGTATCATTTATCATCCTTTTATTCTATAAGTTACCATTGAGACGAGATAAGAAGGCATATTATGACTATGCCGGTTTGTGGCATATATATGGTTTCTTATCCTTGAACAGCTGGTTCTGGAGTGCTGTTTTCCATAGTAGAGATGTGGATTTGACCGAAAAATTAGATTACTCAGCTGCAGTTGCATTACTTGGGTACTCGCTCATTCTTGCCATACTCAGAAGTTTTGATGTGAGGGATGATGCTGTCAGAGTTATGGTTGCTGCTCCGCTGCTTGCTTTTGTAACTACCCACATATTGTATCTCAACTTTTACAAACTGGATTATGGTTGGAACATGAAAGTTTGTGTTGTCATGGCGGTGGCTCAACTTCTTATCTGGGCGGTTTGGGCTGGTGTCACCCGCCATCCATCTCGTTGGAAATTGTGGCTGGTAGTAGTTGGAGGCAGTCTTGCAATGCTCCTAGAAATCTATGATTTCCCCCCATATGAAGGATTTCTGGATGCTCATGCGGTCTGGCATGCCACCACCATCCCCCTAACTTACATTTGGTGGAGTTTCATCAGAGACGACGCTGAGTTCCTAACCTCTAACCAAGTAAAGAAGTTGGCAAAGAAGGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Per1

Protein Analysis

421

Amino Acids

48.65

Weight (kDa)

9.01

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Per1 PF04080 145 - 405 1.5e-98 Per1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 567
AciI CCGC 5 cut(s) 908, 998, 1022, 1042, 1151
AclWI GGATC 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 2 cut(s) 233, 836
AfiI CCNNNNNNNGG 4 cut(s) 90, 411, 717, 1130
AgsI TTSAA 5 cut(s) 12, 23, 143, 562, 745
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 8 cut(s) 68, 128, 136, 281, 521, 599, 750, 815
AluI AGCT 8 cut(s) 68, 128, 136, 281, 521, 599, 750, 815
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 4 cut(s) 192, 406, 673, 1200
AlwI GGATC 1 cut(s) 302
AoxI GGCC 4 cut(s) 33, 92, 413, 554
ApeKI GCWGC 5 cut(s) 125, 518, 815, 902, 910
Asp700I GAANNNNTTC 2 cut(s) 27, 1134
AspLEI GCGC 2 cut(s) 567, 610
AspS9I GGNCC 5 cut(s) 34, 93, 413, 530, 555
AsuHPI GGTGA 2 cut(s) 311, 1030
AvaII GGWCC 1 cut(s) 530
BaeI ACNNNNGTAYC 2 cut(s) 472, 505
BanII GRGCYC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 5 cut(s) 137, 530, 802, 889, 897
BccI CCATC 4 cut(s) 404, 1053, 1057, 1178
BciT130I CCWGG 1 cut(s) 581
BcoDI GTCTC 4 cut(s) 192, 406, 673, 1200
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 4 cut(s) 65, 78, 161, 1103
BfmI CTRYAG 3 cut(s) 282, 594, 816
BisI GCNGC 5 cut(s) 126, 519, 816, 903, 911
BlsI GCNGC 5 cut(s) 127, 520, 817, 904, 912
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 581
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 5 cut(s) 34, 93, 413, 530, 555
BmiI GGNNCC 3 cut(s) 208, 422, 585
BmrFI CCNGG 1 cut(s) 581
BmsI GCATC 6 cut(s) 277, 292, 356, 612, 871, 1132
BpmI CTGGAG 1 cut(s) 778
BsaBI GATNNNNATC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 2 cut(s) 167, 416
BsaWI WCCGGW 2 cut(s) 209, 532
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 411, 717, 1130
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 4 cut(s) 239, 291, 352, 963
Bse3DI GCAATG 1 cut(s) 1101
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 2 cut(s) 167, 416
BseGI GGATG 6 cut(s) 356, 651, 883, 1045, 1147, 1170
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 4 cut(s) 90, 411, 717, 1130
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 3 cut(s) 825, 871, 1206
BseNI ACTGG 4 cut(s) 239, 291, 352, 963
BseXI GCAGC 5 cut(s) 137, 530, 802, 889, 897
Bsh1236I CGCG 1 cut(s) 567
BshFI GGCC 4 cut(s) 35, 94, 415, 556
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 4 cut(s) 91, 210, 533, 711
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 4 cut(s) 90, 411, 717, 1130
BsmAI GTCTC 4 cut(s) 192, 406, 673, 1200
BsmBI CGTCTC 3 cut(s) 192, 673, 1200
BsmFI GGGAC 1 cut(s) 473
BsnI GGCC 4 cut(s) 35, 94, 415, 556
Bso31I GGTCTC 1 cut(s) 406
Bsp1286I GDGCHC 2 cut(s) 60, 217
Bsp143I GATC 1 cut(s) 307
Bsp19I CCATGG 1 cut(s) 416
BspACI CCGC 5 cut(s) 908, 998, 1022, 1042, 1151
BspANI GGCC 4 cut(s) 35, 94, 415, 556
BspCNI CTCAG 3 cut(s) 824, 870, 1207
BspFNI CGCG 1 cut(s) 567
BspLI GGNNCC 3 cut(s) 208, 422, 585
BspMAI CTGCAG 1 cut(s) 820
BspPI GGATC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 1101
BsrFI RCCGGY 1 cut(s) 710
BsrI ACTGG 4 cut(s) 239, 291, 352, 963
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 2 cut(s) 167, 416
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 2 cut(s) 167, 416
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 2 cut(s) 156, 378
Bst6I CTCTTC 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 588, 915, 1161
BstDEI CTNAG 4 cut(s) 118, 811, 857, 1215
BstDSI CCRYGG 1 cut(s) 416
BstF5I GGATG 6 cut(s) 356, 651, 883, 1045, 1147, 1170
BstFNI CGCG 1 cut(s) 567
BstHHI GCGC 2 cut(s) 567, 610
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 4 cut(s) 192, 406, 673, 1200
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 8 cut(s) 287, 302, 562, 596, 605, 821, 1028, 1256
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 1 cut(s) 1163
BstSCI CCNGG 1 cut(s) 579
BstSFI CTRYAG 3 cut(s) 282, 594, 816
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 5 cut(s) 137, 530, 802, 889, 897
BstXI CCANNNNNNTGG 1 cut(s) 1056
BsuRI GGCC 4 cut(s) 35, 94, 415, 556
BtgI CCRYGG 1 cut(s) 416
BtgZI GCGATG 1 cut(s) 635
BtsCI GGATG 6 cut(s) 356, 651, 883, 1045, 1147, 1170
BtsI GCAGTG 1 cut(s) 452
BtsIMutI CAGTG 3 cut(s) 298, 383, 452
Cac8I GCNNGC 3 cut(s) 588, 915, 1161
CfoI GCGC 2 cut(s) 567, 610
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 5 cut(s) 34, 93, 413, 530, 555
CseI GACGC 1 cut(s) 1220
Csp6I GTAC 2 cut(s) 232, 835
CviAII CATG 7 cut(s) 417, 545, 632, 976, 994, 1148, 1160
CviQI GTAC 2 cut(s) 232, 835
DdeI CTNAG 4 cut(s) 118, 811, 857, 1215
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 167, 416
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 2 cut(s) 167, 416
EcoT22I ATGCAT 1 cut(s) 627
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 167, 416
Esp3I CGTCTC 3 cut(s) 192, 673, 1200
FaeI CATG 7 cut(s) 420, 548, 635, 979, 997, 1151, 1163
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 473
FatI CATG 7 cut(s) 416, 544, 631, 975, 993, 1147, 1159
FauI CCCGC 1 cut(s) 1049
FauNDI CATATG 1 cut(s) 1126
Fnu4HI GCNGC 5 cut(s) 126, 519, 816, 903, 911
FokI GGATG 6 cut(s) 363, 638, 890, 1032, 1154, 1157
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 5 cut(s) 126, 519, 816, 903, 911
FspBI CTAG 4 cut(s) 65, 78, 161, 1103
GlaI GCGC 2 cut(s) 566, 609
GluI GCNGC 5 cut(s) 126, 519, 816, 903, 911
GsuI CTGGAG 1 cut(s) 778
HaeIII GGCC 4 cut(s) 35, 94, 415, 556
HapII CCGG 4 cut(s) 91, 210, 533, 711
HgaI GACGC 1 cut(s) 1220
HhaI GCGC 2 cut(s) 567, 610
Hin1II CATG 7 cut(s) 420, 548, 635, 979, 997, 1151, 1163
Hin6I GCGC 2 cut(s) 565, 608
HinP1I GCGC 2 cut(s) 565, 608
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 2 cut(s) 273, 576
HpaII CCGG 4 cut(s) 91, 210, 533, 711
HphI GGTGA 2 cut(s) 311, 1030
Hpy166II GTNNAC 2 cut(s) 329, 496
Hpy188I TCNGA 8 cut(s) 55, 114, 152, 219, 278, 860, 890, 1205
Hpy188III TCNNGA 3 cut(s) 78, 757, 1139
Hpy8I GTNNAC 2 cut(s) 329, 496
Hpy99I CGWCG 1 cut(s) 1214
HpyAV CCTTC 4 cut(s) 568, 685, 1124, 1249
HpyCH4III ACNGT 2 cut(s) 156, 378
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 7 cut(s) 381, 427, 445, 625, 818, 824, 1094
HpyF10VI GCNNNNNNNGC 8 cut(s) 287, 302, 562, 596, 605, 821, 1028, 1256
HpyF3I CTNAG 4 cut(s) 118, 811, 857, 1215
HpySE526I ACGT 1 cut(s) 50
Hsp92II CATG 7 cut(s) 420, 548, 635, 979, 997, 1151, 1163
HspAI GCGC 2 cut(s) 565, 608
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 4 cut(s) 220, 583, 910, 1104
Lsp1109I GCAGC 5 cut(s) 137, 530, 802, 889, 897
LweI GCATC 6 cut(s) 277, 292, 356, 612, 871, 1132
MaeI CTAG 4 cut(s) 65, 78, 161, 1103
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 4 cut(s) 156, 669, 922, 1036
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 8 cut(s) 184, 187, 190, 193, 196, 199, 353, 381
MhlI GDGCHC 2 cut(s) 60, 217
MluCI AATT 4 cut(s) 43, 382, 800, 1061
MmeI TCCRAC 3 cut(s) 950, 1037, 1060
MnlI CCTC 6 cut(s) 197, 321, 444, 867, 1076, 1237
Mph1103I ATGCAT 1 cut(s) 627
MroXI GAANNNNTTC 2 cut(s) 27, 1134
MseI TTAA 2 cut(s) 47, 224
MslI CAYNNNNRTG 4 cut(s) 364, 630, 726, 998
MspA1I CMGCKG 5 cut(s) 128, 521, 750, 815, 910
MspI CCGG 4 cut(s) 91, 210, 533, 711
MspR9I CCNGG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 581
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 8 cut(s) 287, 302, 562, 596, 605, 821, 1028, 1256
NcoI CCATGG 1 cut(s) 416
NdeI CATATG 1 cut(s) 1126
NdeII GATC 1 cut(s) 307
NlaIII CATG 7 cut(s) 420, 548, 635, 979, 997, 1151, 1163
NlaIV GGNNCC 3 cut(s) 208, 422, 585
NmuCI GTSAC 2 cut(s) 156, 1036
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 1 cut(s) 1163
PaeI GCATGC 1 cut(s) 1163
PdmI GAANNNNTTC 2 cut(s) 27, 1134
PfeI GAWTC 2 cut(s) 273, 576
PfoI TCCNGGA 1 cut(s) 579
PkrI GCNGC 5 cut(s) 127, 520, 817, 904, 912
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 3 cut(s) 208, 422, 585
PspPI GGNCC 5 cut(s) 34, 93, 413, 530, 555
PstI CTGCAG 1 cut(s) 820
PvuII CAGCTG 4 cut(s) 128, 521, 750, 815
RsaI GTAC 2 cut(s) 233, 836
RsaNI GTAC 2 cut(s) 232, 835
RseI CAYNNNNRTG 4 cut(s) 364, 630, 726, 998
SaqAI TTAA 2 cut(s) 47, 224
SatI GCNGC 5 cut(s) 126, 519, 816, 903, 911
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 5 cut(s) 34, 93, 413, 530, 555
ScaI AGTACT 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 2 cut(s) 60, 217
SfaNI GCATC 6 cut(s) 277, 292, 356, 612, 871, 1132
SfcI CTRYAG 3 cut(s) 282, 594, 816
SinI GGWCC 1 cut(s) 530
SmiMI CAYNNNNRTG 4 cut(s) 364, 630, 726, 998
SpeI ACTAGT 1 cut(s) 160
SphI GCATGC 1 cut(s) 1163
Sse9I AATT 4 cut(s) 43, 382, 800, 1061
SsiI CCGC 5 cut(s) 908, 998, 1022, 1042, 1151
SspMI CTAG 4 cut(s) 65, 78, 161, 1103
StyD4I CCNGG 1 cut(s) 579
StyI CCWWGG 2 cut(s) 167, 416
TaaI ACNGT 2 cut(s) 156, 378
TaiI ACGT 1 cut(s) 53
TaqII GACCGA 1 cut(s) 809
TasI AATT 4 cut(s) 43, 382, 800, 1061
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 2 cut(s) 273, 576
Tru1I TTAA 2 cut(s) 47, 224
Tru9I TTAA 2 cut(s) 47, 224
TscAI CASTG 3 cut(s) 298, 383, 452
TseFI GTSAC 2 cut(s) 156, 1036
TseI GCWGC 5 cut(s) 125, 518, 815, 902, 910
Tsp45I GTSAC 2 cut(s) 156, 1036
TspDTI ATGAA 4 cut(s) 620, 992, 1143, 1189
TspGWI ACGGA 2 cut(s) 216, 394
TspRI CASTG 3 cut(s) 298, 383, 452
VpaK11BI GGWCC 1 cut(s) 530
XbaI TCTAGA 1 cut(s) 77
XceI RCATGY 1 cut(s) 1163
XmnI GAANNNNTTC 2 cut(s) 27, 1134
XspI CTAG 4 cut(s) 65, 78, 161, 1103
ZrmI AGTACT 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.