Rh5CG200900

post-GPI attachment to proteins factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007052
Physical Location & Seq
Forward (+)
20176749 .. 20181222
4474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG200900.1

Sequence Viewer

Length: 1269 bp
ATGTGGTCCAGGTTTGGGTTGACTTGTTCAAGGTTGAACGAGTTTCAGGCCCAGACTAATTTAACGTCCGAGCCCAAACTAGCTTTACATTCTCGACCCGTTTTCCGGCCCAAACCCGAAATCAATCTGAACTTAGAAGCAGCTGTTGAGCTTTTTGAATGGGTCAGACTGACACTATTGCCAAGGAAGAAGAAAGAGAGAGACAGAGGTTCCGGTGCTCCGATGTTAAAGCAGTACTGGGTTGCTTTCTTTGTGGTGGTTTTATGGGGTTTTGGATTCAGAGCTATAGATGCCAGTGCTGGTGATGCTGATCCTCATTATAGGGCTTGTTTACAACAATGTGAAGAAACTGGATGTGTAGCGCAGAGATGCTTTCCACATTGCAATTTCTCTTCAGGTGGGGTTTCCGTTGATGGGCCATGGTTCCTGCAAGAACCTCTTTATTTGCAGTGGAAACAATGGGACTGTCAGAGTGATTGCCGTTACTATTGTATGGTTGACAGAGAGAAAGAAAGAGAAGCAGCTGGTTATGGCCCAGTCAAATATCATGGTAAATGGCCCTTCAAGCGCGTTTATGGAATCCAGGAGCCTGCTTCTGTAGCTTTCTCTGCGCTCAACCTTGCTATGCATTTTCATGGTTGGGTATCACTTATCATCCTTTTATTCTATAAGTTACCATTGAGACGAGATAAGAAGGCATATTATGACTATGCCGGTTTGTGGCATATATATGGTTTCTTATCCTTGAACAGCTGGTTCTGGAGTGCTGTTTTCCATAGTAGAGATGTGGATTGGACCGAAAAATTAGATTACTCAGCTGCAGTGGCATTACTTGGGTACTCGCTCATTCTTGCCATACTCAGAAGTTTTGACGTGAGGGATGATGCTGTCAGAGTTATGGTTGCTGCTCCACTGCTTGCTTTTGTAACTACCCACATATTGTATCTCAACTTTTATAAACTGGATTATGGTTGGAACATGAAAGTTTGTGTTGTCATGGCGGTGGCTCAACTTCTTATCTGGGCGGTTTGGGCTGGTGTCACCCACCATCCATCTCGTTGGAAATTGTGGCTGGTAGTAGTTGGAGGCGGTCTTGCAATGCTCCTAGAAATCTATGATTTCCCCCCATATGAAGGATTTCTGGATGCTCATGCGGTCTGGCATGCCACCACCATCCCCCTAACTTACATTTGGTGGAGTTTCATCAGAGACGATGCTGAGTTCCTAACCTCTAACCAAGTAAAGAAGCTGGCAAAGAAGGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Per1

Protein Analysis

422

Amino Acids

48.86

Weight (kDa)

8.76

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Per1 PF04080 146 - 406 3e-98 Per1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 957
AccII CGCG 1 cut(s) 570
AciI CCGC 4 cut(s) 1001, 1025, 1089, 1154
AclWI GGATC 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 2 cut(s) 236, 839
AfiI CCNNNNNNNGG 5 cut(s) 15, 105, 414, 720, 1133
AgsI TTSAA 5 cut(s) 30, 37, 158, 565, 748
AjiI CACGTC 1 cut(s) 874
AjnI CCWGG 2 cut(s) 8, 582
AluBI AGCT 9 cut(s) 83, 143, 151, 284, 524, 602, 753, 818, 1249
AluI AGCT 9 cut(s) 83, 143, 151, 284, 524, 602, 753, 818, 1249
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 3 cut(s) 195, 676, 1203
AlwI GGATC 1 cut(s) 305
AoxI GGCC 5 cut(s) 48, 107, 416, 532, 557
ApeKI GCWGC 4 cut(s) 140, 521, 818, 905
Asp700I GAANNNNTTC 1 cut(s) 1137
AspLEI GCGC 3 cut(s) 364, 570, 613
AspS9I GGNCC 7 cut(s) 6, 49, 108, 416, 533, 558, 795
AsuHPI GGTGA 2 cut(s) 314, 1033
AvaII GGWCC 2 cut(s) 6, 795
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 4 cut(s) 152, 533, 805, 892
BccI CCATC 4 cut(s) 407, 1056, 1060, 1181
BceAI ACGGC 1 cut(s) 465
BciT130I CCWGG 2 cut(s) 10, 584
BcoDI GTCTC 3 cut(s) 195, 676, 1203
BfaI CTAG 2 cut(s) 80, 1106
BfmI CTRYAG 3 cut(s) 285, 597, 819
BisI GCNGC 4 cut(s) 141, 522, 819, 906
BlsI GCNGC 4 cut(s) 142, 523, 820, 907
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 2 cut(s) 10, 584
Bme18I GGWCC 2 cut(s) 6, 795
BmgBI CACGTC 1 cut(s) 874
BmgT120I GGNCC 7 cut(s) 6, 49, 108, 416, 533, 558, 795
BmiI GGNNCC 3 cut(s) 211, 425, 588
BmrFI CCNGG 2 cut(s) 10, 584
BmrI ACTGGG 2 cut(s) 247, 530
BmsI GCATC 6 cut(s) 280, 295, 359, 874, 1135, 1204
BmuI ACTGGG 2 cut(s) 247, 530
BpmI CTGGAG 1 cut(s) 781
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 182, 419
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 105, 414, 720, 1133
Bse118I RCCGGY 1 cut(s) 713
Bse1I ACTGG 5 cut(s) 242, 294, 355, 536, 966
Bse3DI GCAATG 2 cut(s) 379, 1104
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 2 cut(s) 10, 584
BseDI CCNNGG 2 cut(s) 182, 419
BseGI GGATG 6 cut(s) 359, 654, 886, 1048, 1150, 1173
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 5 cut(s) 15, 105, 414, 720, 1133
BseMI GCAATG 2 cut(s) 379, 1104
BseMII CTCAG 3 cut(s) 828, 874, 1209
BseNI ACTGG 5 cut(s) 242, 294, 355, 536, 966
BseXI GCAGC 4 cut(s) 152, 533, 805, 892
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 5 cut(s) 50, 109, 418, 534, 559
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 3 cut(s) 106, 213, 714
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 5 cut(s) 15, 105, 414, 720, 1133
BsmAI GTCTC 3 cut(s) 195, 676, 1203
BsmBI CGTCTC 2 cut(s) 676, 1203
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 5 cut(s) 50, 109, 418, 534, 559
Bsp1286I GDGCHC 2 cut(s) 75, 220
Bsp143I GATC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 4 cut(s) 1001, 1025, 1089, 1154
BspANI GGCC 5 cut(s) 50, 109, 418, 534, 559
BspCNI CTCAG 3 cut(s) 827, 873, 1210
BspFNI CGCG 1 cut(s) 570
BspLI GGNNCC 3 cut(s) 211, 425, 588
BspMAI CTGCAG 1 cut(s) 823
BspPI GGATC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 379, 1104
BsrFI RCCGGY 1 cut(s) 713
BsrI ACTGG 5 cut(s) 242, 294, 355, 536, 966
BssAI RCCGGY 1 cut(s) 713
BssECI CCNNGG 2 cut(s) 182, 419
BssMI GATC 1 cut(s) 310
BssT1I CCWWGG 2 cut(s) 182, 419
Bst2UI CCWGG 2 cut(s) 10, 584
Bst4CI ACNGT 1 cut(s) 467
Bst6I CTCTTC 1 cut(s) 397
BstC8I GCNNGC 4 cut(s) 591, 918, 1164, 1251
BstDEI CTNAG 4 cut(s) 133, 814, 860, 1218
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 6 cut(s) 359, 654, 886, 1048, 1150, 1173
BstFNI CGCG 1 cut(s) 570
BstHHI GCGC 3 cut(s) 364, 570, 613
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 3 cut(s) 195, 676, 1203
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 8 cut(s) 290, 305, 565, 599, 608, 824, 1031, 1259
BstNI CCWGG 2 cut(s) 10, 584
BstNSI RCATGY 1 cut(s) 1166
BstSCI CCNGG 2 cut(s) 8, 582
BstSFI CTRYAG 3 cut(s) 285, 597, 819
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 4 cut(s) 152, 533, 805, 892
BstXI CCANNNNNNTGG 1 cut(s) 1059
BsuRI GGCC 5 cut(s) 50, 109, 418, 534, 559
BtgI CCRYGG 1 cut(s) 419
BtrI CACGTC 1 cut(s) 874
BtsCI GGATG 6 cut(s) 359, 654, 886, 1048, 1150, 1173
BtsI GCAGTG 3 cut(s) 455, 828, 911
BtsIMutI CAGTG 4 cut(s) 301, 455, 828, 911
Cac8I GCNNGC 4 cut(s) 591, 918, 1164, 1251
CfoI GCGC 3 cut(s) 364, 570, 613
Cfr10I RCCGGY 1 cut(s) 713
Cfr13I GGNCC 7 cut(s) 6, 49, 108, 416, 533, 558, 795
Csp6I GTAC 2 cut(s) 235, 838
CviAII CATG 7 cut(s) 420, 548, 635, 979, 997, 1151, 1163
CviQI GTAC 2 cut(s) 235, 838
DdeI CTNAG 4 cut(s) 133, 814, 860, 1218
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 182, 419
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 6, 795
Eco57I CTGAAG 1 cut(s) 378
EcoRII CCWGG 2 cut(s) 8, 582
EcoT14I CCWWGG 2 cut(s) 182, 419
EcoT22I ATGCAT 1 cut(s) 630
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 2 cut(s) 182, 419
Esp3I CGTCTC 2 cut(s) 676, 1203
FaeI CATG 7 cut(s) 423, 551, 638, 982, 1000, 1154, 1166
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 1 cut(s) 476
FatI CATG 7 cut(s) 419, 547, 634, 978, 996, 1150, 1162
FauNDI CATATG 1 cut(s) 1129
Fnu4HI GCNGC 4 cut(s) 141, 522, 819, 906
FokI GGATG 6 cut(s) 366, 641, 893, 1035, 1157, 1160
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 4 cut(s) 141, 522, 819, 906
FspBI CTAG 2 cut(s) 80, 1106
GlaI GCGC 3 cut(s) 363, 569, 612
GluI GCNGC 4 cut(s) 141, 522, 819, 906
GsuI CTGGAG 1 cut(s) 781
HaeIII GGCC 5 cut(s) 50, 109, 418, 534, 559
HapII CCGG 3 cut(s) 106, 213, 714
HhaI GCGC 3 cut(s) 364, 570, 613
Hin1II CATG 7 cut(s) 423, 551, 638, 982, 1000, 1154, 1166
Hin6I GCGC 3 cut(s) 362, 568, 611
HinP1I GCGC 3 cut(s) 362, 568, 611
HincII GTYRAC 2 cut(s) 21, 499
HindII GTYRAC 2 cut(s) 21, 499
HinfI GANTC 2 cut(s) 276, 579
HpaII CCGG 3 cut(s) 106, 213, 714
HphI GGTGA 2 cut(s) 314, 1033
Hpy166II GTNNAC 3 cut(s) 21, 332, 499
Hpy188I TCNGA 9 cut(s) 70, 129, 167, 222, 281, 471, 863, 893, 1208
Hpy188III TCNNGA 3 cut(s) 93, 760, 1142
Hpy8I GTNNAC 3 cut(s) 21, 332, 499
HpyAV CCTTC 4 cut(s) 571, 688, 1127, 1252
HpyCH4III ACNGT 1 cut(s) 467
HpyCH4IV ACGT 2 cut(s) 65, 873
HpyCH4V TGCA 6 cut(s) 384, 430, 448, 628, 821, 1097
HpyF10VI GCNNNNNNNGC 8 cut(s) 290, 305, 565, 599, 608, 824, 1031, 1259
HpyF3I CTNAG 4 cut(s) 133, 814, 860, 1218
HpySE526I ACGT 2 cut(s) 65, 873
Hsp92II CATG 7 cut(s) 423, 551, 638, 982, 1000, 1154, 1166
HspAI GCGC 3 cut(s) 362, 568, 611
Kzo9I GATC 1 cut(s) 310
LmnI GCTCC 4 cut(s) 223, 586, 913, 1107
Lsp1109I GCAGC 4 cut(s) 152, 533, 805, 892
LweI GCATC 6 cut(s) 280, 295, 359, 874, 1135, 1204
MaeI CTAG 2 cut(s) 80, 1106
MaeII ACGT 2 cut(s) 65, 873
MaeIII GTNAC 4 cut(s) 482, 672, 925, 1039
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 4 cut(s) 199, 202, 356, 384
MhlI GDGCHC 2 cut(s) 75, 220
MluCI AATT 4 cut(s) 58, 385, 803, 1064
MmeI TCCRAC 3 cut(s) 953, 1040, 1063
MnlI CCTC 6 cut(s) 200, 324, 447, 870, 1079, 1240
Mph1103I ATGCAT 1 cut(s) 630
MroXI GAANNNNTTC 1 cut(s) 1137
MseI TTAA 2 cut(s) 62, 227
MslI CAYNNNNRTG 3 cut(s) 633, 729, 1001
MspA1I CMGCKG 4 cut(s) 143, 524, 753, 818
MspI CCGG 3 cut(s) 106, 213, 714
MspR9I CCNGG 2 cut(s) 10, 584
MvaI CCWGG 2 cut(s) 10, 584
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 8 cut(s) 290, 305, 565, 599, 608, 824, 1031, 1259
NcoI CCATGG 1 cut(s) 419
NdeI CATATG 1 cut(s) 1129
NdeII GATC 1 cut(s) 310
NlaIII CATG 7 cut(s) 423, 551, 638, 982, 1000, 1154, 1166
NlaIV GGNNCC 3 cut(s) 211, 425, 588
NmuCI GTSAC 1 cut(s) 1039
NsiI ATGCAT 1 cut(s) 630
NspI RCATGY 1 cut(s) 1166
PaeI GCATGC 1 cut(s) 1166
PdmI GAANNNNTTC 1 cut(s) 1137
PfeI GAWTC 2 cut(s) 276, 579
PfoI TCCNGGA 1 cut(s) 582
PkrI GCNGC 4 cut(s) 142, 523, 820, 907
PsiI TTATAA 1 cut(s) 957
Psp6I CCWGG 2 cut(s) 8, 582
PspGI CCWGG 2 cut(s) 8, 582
PspN4I GGNNCC 3 cut(s) 211, 425, 588
PspPI GGNCC 7 cut(s) 6, 49, 108, 416, 533, 558, 795
PstI CTGCAG 1 cut(s) 823
PvuII CAGCTG 4 cut(s) 143, 524, 753, 818
RsaI GTAC 2 cut(s) 236, 839
RsaNI GTAC 2 cut(s) 235, 838
RseI CAYNNNNRTG 3 cut(s) 633, 729, 1001
SaqAI TTAA 2 cut(s) 62, 227
SatI GCNGC 4 cut(s) 141, 522, 819, 906
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 7 cut(s) 6, 49, 108, 416, 533, 558, 795
ScaI AGTACT 1 cut(s) 236
ScrFI CCNGG 2 cut(s) 10, 584
SduI GDGCHC 2 cut(s) 75, 220
SfaNI GCATC 6 cut(s) 280, 295, 359, 874, 1135, 1204
SfcI CTRYAG 3 cut(s) 285, 597, 819
SinI GGWCC 2 cut(s) 6, 795
SmiMI CAYNNNNRTG 3 cut(s) 633, 729, 1001
SphI GCATGC 1 cut(s) 1166
Sse9I AATT 4 cut(s) 58, 385, 803, 1064
SsiI CCGC 4 cut(s) 1001, 1025, 1089, 1154
SspMI CTAG 2 cut(s) 80, 1106
StyD4I CCNGG 2 cut(s) 8, 582
StyI CCWWGG 2 cut(s) 182, 419
TaaI ACNGT 1 cut(s) 467
TaiI ACGT 2 cut(s) 68, 876
TaqI TCGA 1 cut(s) 94
TaqII GACCGA 1 cut(s) 812
TasI AATT 4 cut(s) 58, 385, 803, 1064
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 2 cut(s) 276, 579
Tru1I TTAA 2 cut(s) 62, 227
Tru9I TTAA 2 cut(s) 62, 227
TscAI CASTG 4 cut(s) 301, 455, 828, 918
TseFI GTSAC 1 cut(s) 1039
TseI GCWGC 4 cut(s) 140, 521, 818, 905
Tsp45I GTSAC 1 cut(s) 1039
TspDTI ATGAA 4 cut(s) 623, 995, 1146, 1192
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 4 cut(s) 301, 455, 828, 918
VpaK11BI GGWCC 2 cut(s) 6, 795
XceI RCATGY 1 cut(s) 1166
XmnI GAANNNNTTC 1 cut(s) 1137
XspI CTAG 2 cut(s) 80, 1106
ZrmI AGTACT 1 cut(s) 236
Zsp2I ATGCAT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.