MD12G1014900.v1.1

Regulation of nuclear pre-mRNA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008113
Physical Location & Seq
Reverse (-)
1521816 .. 1534689
12874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1014900.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGAGTAACGTTGAACCTTTTGACGGACAGATATTGGCTGACAAGCTTTCAAAACTCAACGGTTCACAACAATGCATTGAATCTTTATCTCGTTGGTGTATTTCTCACCGAAAGAAAGCAAGGCAGATTGTTGAAACGTGGGATAAATGCTTTAATTCTGCCCAGAAAGATCAGCGCGTGGCTTGTCTATATTTGGCTAATGACATATTGCAAAACAGTAGGCGAAAGGGCAGTGAATTTGTGAATGAATTCTGGAAGTTCCTTCCTGCAGCTCTTAAGCATGTTTATGAAAATGGTGATCAACATGGAAAGAAAGCTGCCACAAGACTGGTTGACATATGGGAAGAAAGAAAGGTTTTTGGGTCTCGAGGGCATAGTCTTAAAGATGATATGATGGGAAGGAATCCTCCTGCTCCACCTGCTCCACCTGCAAGCAATGGAAAGAGTTCAAATCCTATCAAGATAGTGAAGAGGGATGCACACTCAGTTAGACTTAAATTGGCTGTTGGAGGTCTGCCAGAAAAAATACTTACTGCATTTCAACCTGTTCTTGATGAACATCTTACGGAAGAAGCTGCTTTACACAAGTGTAATGCTGCTGTACTTTATGTGGGTAAAATTGATGAAGATGTTGAAAACACCTTGATTCATGGAACTCAGCAGGGATCCACATTGTTGGATGATTTGAAAGAGCAAGAAGATGCACTTAATCAATCTGTTGGGCAACTTGAGAGCTCGGAGGCAACAAGATCTGCCTTGGTTTCGCAACTTAAAGAAGCACTTCAGGATCAAGAAGCAAAGCTGGAAGTTGTTCGCACTCAATTGCAGGTTGCTCGACATCAGATTGAGAGAGTAAGTAATATTAAAAGGAGACTGAGTTTGTCCCCGGTTCTGAACCCCGAAAGCAATGTTGTTAACATGACACCTGAATCTACCAGGGGGTTAGAACCAAATTTACCGTTAGTCCAACAAACTGGCCTCCTACCACAGCCTCCAACGCAGCCTATGGTTTCTTTTGCCCCTGTAAAAACTACCGATGAAGAGAACAAGAAAGCAGCAGCAGCAGCTGTGGCTGCTAAGCTTGCTGCATCTACATCTTCTGCACAGATGTTAACGTCTGTTCTTTCATCTCTTGTTGCTGAAGAAGCTGCCTCTATGACTGGTAGCCTGAAATCAGCTGGTTTTACATCAGGATTATCAATGTTTCCTCCAGAAAAATGGCCCAAGCTTGAGAAACAAATGTCTGATGTTAACAATCCTGATGGTGGCAATGCAGCCTTTTTTACGCCTCTCCAACAGCAGGCAATGACCAGTGTTCCAATTGCGTCATCAGCAGCCATGCAGCCTATGTCCCACAGTAACCAGATGCAAAGTCCTTTTAGTCCGCTGCCACCACCTCCGGCACCATCACCACCTACTGCAACTACACCAGCGAATCAGTATGTCCAATCTTCTGGTCTTATGGTGGGTGGAATTCCTTACGGATATGGATCAAATACCTTGCCGCCTCCACCCCCTATACCTCCACATATCTCAATGAGTATGTCAAGGCCCAGTCAGCCGCAACAGCAGTCGCAGCCTCAGCAGCAGCAGCAGTTGCAGCCGGCTGCTGGGGGTTATTATCGGCCACTGGGTATGGGGTTCTACGGGCAAAGTAATCAGTCAAATACGCAGCCAGTAGTACCTCGACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

565

Amino Acids

61.29

Weight (kDa)

8.42

Isoelectric Point (pI)

63.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 11 - 122 1e-37 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 427, 436
Acc36I ACCTGC 3 cut(s) 427, 436, 817
AccB1I GGYRCC 1 cut(s) 1402
AccII CGCG 1 cut(s) 177
AciI CCGC 3 cut(s) 1385, 1505, 1562
AclI AACGTT 1 cut(s) 9
AclWI GGATC 4 cut(s) 660, 673, 795, 1498
AcoI YGGCCR 1 cut(s) 1625
AcsI RAATTY 4 cut(s) 236, 248, 952, 1473
AcuI CTGAAG 2 cut(s) 767, 1161
AfaI GTAC 2 cut(s) 603, 1683
AfiI CCNNNNNNNGG 4 cut(s) 23, 1265, 1300, 1610
AflII CTTAAG 1 cut(s) 275
AgsI TTSAA 8 cut(s) 14, 51, 80, 134, 450, 542, 635, 688
AjnI CCWGG 1 cut(s) 935
Alw21I GWGCWC 1 cut(s) 737
Alw26I GTCTC 2 cut(s) 369, 865
AlwI GGATC 4 cut(s) 660, 673, 795, 1498
AlwNI CAGNNNCTG 1 cut(s) 1067
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 4 cut(s) 976, 1220, 1550, 1625
ApoI RAATTY 4 cut(s) 236, 248, 952, 1473
Asp700I GAANNNNTTC 4 cut(s) 248, 445, 780, 810
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 2 cut(s) 1221, 1551
AsuC2I CCSGG 1 cut(s) 887
AsuHPI GGTGA 3 cut(s) 98, 308, 1401
AvaI CYCGRG 1 cut(s) 366
BamHI GGATCC 1 cut(s) 665
BanI GGYRCC 1 cut(s) 1402
BanII GRGCYC 1 cut(s) 737
BarI GAAGNNNNNNTAC 2 cut(s) 564, 596
Bbv12I GWGCWC 1 cut(s) 737
BbvCI CCTCAGC 1 cut(s) 1581
BccI CCATC 3 cut(s) 388, 1256, 1414
BcgI CGANNNNNNTGC 2 cut(s) 815, 849
BciT130I CCWGG 1 cut(s) 937
BclI TGATCA 1 cut(s) 298
BcnI CCSGG 1 cut(s) 887
BcoDI GTCTC 2 cut(s) 369, 865
BfmI CTRYAG 1 cut(s) 267
BfrI CTTAAG 1 cut(s) 275
BfuAI ACCTGC 3 cut(s) 427, 436, 817
BglII AGATCT 1 cut(s) 749
BlpI GCTNAGC 1 cut(s) 1077
Bme1390I CCNGG 2 cut(s) 887, 937
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 2 cut(s) 1221, 1551
BmiI GGNNCC 2 cut(s) 667, 1404
BmrFI CCNGG 2 cut(s) 887, 937
BmrI ACTGGG 2 cut(s) 1548, 1640
BmsI GCATC 4 cut(s) 466, 691, 1097, 1356
BmuI ACTGGG 2 cut(s) 1548, 1640
BpmI CTGGAG 1 cut(s) 1194
Bpu10I CCTNAGC 1 cut(s) 1581
Bpu1102I GCTNAGC 1 cut(s) 1077
BpuEI CTTGAG 2 cut(s) 749, 1250
BpuMI CCSGG 1 cut(s) 887
BsaBI GATNNNNATC 2 cut(s) 558, 1489
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 756, 885, 936
BsaXI ACNNNNNCTCC 2 cut(s) 862, 892
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 1265, 1300, 1610
Bse118I RCCGGY 1 cut(s) 1603
Bse1I ACTGG 7 cut(s) 333, 979, 1165, 1311, 1554, 1635, 1676
Bse3DI GCAATG 4 cut(s) 442, 913, 1276, 1311
Bse8I GATNNNNATC 2 cut(s) 558, 1489
BseBI CCWGG 1 cut(s) 937
BseDI CCNNGG 3 cut(s) 756, 885, 936
BseGI GGATG 2 cut(s) 481, 685
BseJI GATNNNNATC 2 cut(s) 558, 1489
BseLI CCNNNNNNNGG 4 cut(s) 23, 1265, 1300, 1610
BseMI GCAATG 4 cut(s) 442, 913, 1276, 1311
BseMII CTCAG 4 cut(s) 498, 671, 866, 1595
BseNI ACTGG 7 cut(s) 333, 979, 1165, 1311, 1554, 1635, 1676
BseYI CCCAGC 1 cut(s) 1610
BsgI GTGCAG 1 cut(s) 1086
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 4 cut(s) 978, 1222, 1552, 1627
BshNI GGYRCC 1 cut(s) 1402
BsiHKAI GWGCWC 1 cut(s) 737
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 3 cut(s) 887, 1400, 1604
BslFI GGGAC 2 cut(s) 868, 1336
BslI CCNNNNNNNGG 4 cut(s) 23, 1265, 1300, 1610
BsmAI GTCTC 2 cut(s) 369, 865
BsmFI GGGAC 2 cut(s) 868, 1336
BsnI GGCC 4 cut(s) 978, 1222, 1552, 1627
Bso31I GGTCTC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 1 cut(s) 737
Bsp143I GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
Bsp1720I GCTNAGC 1 cut(s) 1077
BspACI CCGC 3 cut(s) 1385, 1505, 1562
BspANI GGCC 4 cut(s) 978, 1222, 1552, 1627
BspCNI CTCAG 4 cut(s) 497, 670, 867, 1594
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 2 cut(s) 667, 1404
BspMAI CTGCAG 1 cut(s) 271
BspMI ACCTGC 3 cut(s) 427, 436, 817
BspPI GGATC 4 cut(s) 660, 673, 795, 1498
BspT107I GGYRCC 1 cut(s) 1402
BspTI CTTAAG 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 369
BsrDI GCAATG 4 cut(s) 442, 913, 1276, 1311
BsrFI RCCGGY 1 cut(s) 1603
BsrI ACTGG 7 cut(s) 333, 979, 1165, 1311, 1554, 1635, 1676
BssAI RCCGGY 1 cut(s) 1603
BssECI CCNNGG 3 cut(s) 756, 885, 936
BssMI GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
BssT1I CCWWGG 1 cut(s) 756
Bst2UI CCWGG 1 cut(s) 937
Bst4CI ACNGT 5 cut(s) 62, 218, 960, 1358, 1692
Bst6I CTCTTC 2 cut(s) 464, 1035
BstAFI CTTAAG 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 1597
BstC8I GCNNGC 4 cut(s) 433, 1083, 1302, 1605
BstDEI CTNAG 5 cut(s) 484, 657, 875, 1077, 1581
BstF5I GGATG 2 cut(s) 481, 685
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 6 cut(s) 172, 301, 668, 752, 790, 1493
BstMAI GTCTC 2 cut(s) 369, 865
BstMBI GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
BstNI CCWGG 1 cut(s) 937
BstNSI RCATGY 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 885, 935
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 1 cut(s) 177
BstX2I RGATCY 2 cut(s) 665, 749
BstXI CCANNNNNNTGG 5 cut(s) 328, 676, 974, 1218, 1454
BstYI RGATCY 2 cut(s) 665, 749
BsuRI GGCC 4 cut(s) 978, 1222, 1552, 1627
BtsCI GGATG 2 cut(s) 481, 685
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 3 cut(s) 238, 1318, 1628
BveI ACCTGC 3 cut(s) 427, 436, 817
Cac8I GCNNGC 4 cut(s) 433, 1083, 1302, 1605
CaiI CAGNNNCTG 1 cut(s) 1067
CfoI GCGC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 1603
Cfr13I GGNCC 2 cut(s) 1221, 1551
CseI GACGC 1 cut(s) 1314
Csp6I GTAC 2 cut(s) 602, 1682
CviAII CATG 5 cut(s) 281, 305, 650, 919, 1339
CviQI GTAC 2 cut(s) 602, 1682
DdeI CTNAG 5 cut(s) 484, 657, 875, 1077, 1581
DpnI GATC 6 cut(s) 171, 300, 667, 751, 789, 1492
DpnII GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
EaeI YGGCCR 1 cut(s) 1625
Eam1104I CTCTTC 2 cut(s) 464, 1035
EarI CTCTTC 2 cut(s) 464, 1035
Ecl136II GAGCTC 1 cut(s) 735
Eco130I CCWWGG 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 735
Eco57I CTGAAG 2 cut(s) 767, 1161
Eco88I CYCGRG 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 735
EcoRI GAATTC 2 cut(s) 248, 1473
EcoRII CCWGG 1 cut(s) 935
EcoT14I CCWWGG 1 cut(s) 756
EcoT22I ATGCAT 1 cut(s) 77
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 5 cut(s) 284, 308, 653, 922, 1342
FalI AAGNNNNNCTT 4 cut(s) 690, 722, 765, 797
FaqI GGGAC 2 cut(s) 868, 1336
FatI CATG 5 cut(s) 280, 304, 649, 918, 1338
FauNDI CATATG 1 cut(s) 338
FbaI TGATCA 1 cut(s) 298
FokI GGATG 2 cut(s) 488, 692
FriOI GRGCYC 1 cut(s) 737
GlaI GCGC 1 cut(s) 176
GsaI CCCAGC 1 cut(s) 1614
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 4 cut(s) 978, 1222, 1552, 1627
HapII CCGG 3 cut(s) 887, 1400, 1604
HgaI GACGC 1 cut(s) 1314
HhaI GCGC 1 cut(s) 177
Hin1II CATG 5 cut(s) 284, 308, 653, 922, 1342
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 4 cut(s) 334, 916, 1113, 1252
HindII GTYRAC 4 cut(s) 334, 916, 1113, 1252
HindIII AAGCTT 3 cut(s) 44, 1079, 1226
HinfI GANTC 5 cut(s) 80, 403, 646, 929, 1435
HpaI GTTAAC 3 cut(s) 916, 1113, 1252
HpaII CCGG 3 cut(s) 887, 1400, 1604
HphI GGTGA 3 cut(s) 98, 308, 1401
Hpy166II GTNNAC 5 cut(s) 65, 334, 916, 1113, 1252
Hpy188I TCNGA 4 cut(s) 739, 843, 894, 1246
Hpy188III TCNNGA 9 cut(s) 253, 366, 460, 551, 785, 791, 1191, 1211, 1259
Hpy8I GTNNAC 5 cut(s) 65, 334, 916, 1113, 1252
HpyAV CCTTC 2 cut(s) 272, 393
HpyCH4III ACNGT 5 cut(s) 62, 218, 960, 1358, 1692
HpyCH4IV ACGT 3 cut(s) 9, 137, 1115
HpyF3I CTNAG 5 cut(s) 484, 657, 875, 1077, 1581
HpySE526I ACGT 3 cut(s) 9, 137, 1115
Hsp92II CATG 5 cut(s) 284, 308, 653, 922, 1342
HspAI GCGC 1 cut(s) 175
KroI GCCGGC 1 cut(s) 1603
KroNI GCCGGC 1 cut(s) 1605
Ksp22I TGATCA 1 cut(s) 298
KspAI GTTAAC 3 cut(s) 916, 1113, 1252
Kzo9I GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
LmnI GCTCC 2 cut(s) 418, 427
LweI GCATC 4 cut(s) 466, 691, 1097, 1356
MaeII ACGT 3 cut(s) 9, 137, 1115
MaeIII GTNAC 2 cut(s) 5, 1358
MalI GATC 6 cut(s) 171, 300, 667, 751, 789, 1492
MboI GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
MboII GAAGA 9 cut(s) 356, 481, 581, 638, 710, 1052, 1089, 1154, 1443
MfeI CAATTG 2 cut(s) 821, 1320
MflI RGATCY 2 cut(s) 665, 749
MhlI GDGCHC 1 cut(s) 737
MluCI AATT 9 cut(s) 154, 236, 248, 497, 618, 821, 952, 1320, 1473
MmeI TCCRAC 5 cut(s) 487, 657, 991, 1019, 1318
Mph1103I ATGCAT 1 cut(s) 77
MroNI GCCGGC 1 cut(s) 1603
MroXI GAANNNNTTC 4 cut(s) 248, 445, 780, 810
MslI CAYNNNNRTG 2 cut(s) 70, 285
MspA1I CMGCKG 3 cut(s) 1067, 1178, 1387
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 3 cut(s) 887, 1400, 1604
MspR9I CCNGG 2 cut(s) 887, 937
MunI CAATTG 2 cut(s) 821, 1320
MvaI CCWGG 1 cut(s) 937
MvnI CGCG 1 cut(s) 177
NaeI GCCGGC 1 cut(s) 1605
NciI CCSGG 1 cut(s) 887
NdeI CATATG 1 cut(s) 338
NdeII GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
NgoMIV GCCGGC 1 cut(s) 1603
NlaIII CATG 5 cut(s) 284, 308, 653, 922, 1342
NlaIV GGNNCC 2 cut(s) 667, 1404
NsiI ATGCAT 1 cut(s) 77
NspI RCATGY 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 366
PaqCI CACCTGC 2 cut(s) 427, 436
PdiI GCCGGC 1 cut(s) 1605
PdmI GAANNNNTTC 4 cut(s) 248, 445, 780, 810
PfeI GAWTC 5 cut(s) 80, 403, 646, 929, 1435
Psp124BI GAGCTC 1 cut(s) 737
Psp1406I AACGTT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 935
PspFI CCCAGC 1 cut(s) 1610
PspGI CCWGG 1 cut(s) 935
PspN4I GGNNCC 2 cut(s) 667, 1404
PspPI GGNCC 2 cut(s) 1221, 1551
PsrI GAACNNNNNNTAC 2 cut(s) 939, 971
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 1067
PsuI RGATCY 2 cut(s) 665, 749
PvuII CAGCTG 2 cut(s) 1067, 1178
RsaI GTAC 2 cut(s) 603, 1683
RsaNI GTAC 2 cut(s) 602, 1682
RseI CAYNNNNRTG 2 cut(s) 70, 285
SacI GAGCTC 1 cut(s) 737
Sau3AI GATC 6 cut(s) 169, 298, 665, 749, 787, 1490
Sau96I GGNCC 2 cut(s) 1221, 1551
ScrFI CCNGG 2 cut(s) 887, 937
SduI GDGCHC 1 cut(s) 737
SfaNI GCATC 4 cut(s) 466, 691, 1097, 1356
SfcI CTRYAG 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 366
SlaI CTCGAG 1 cut(s) 366
SmiMI CAYNNNNRTG 2 cut(s) 70, 285
SmlI CTYRAG 4 cut(s) 275, 366, 728, 1229
SmoI CTYRAG 4 cut(s) 275, 366, 728, 1229
Sse9I AATT 9 cut(s) 154, 236, 248, 497, 618, 821, 952, 1320, 1473
SsiI CCGC 3 cut(s) 1385, 1505, 1562
SspI AATATT 1 cut(s) 862
SstI GAGCTC 1 cut(s) 737
StyD4I CCNGG 2 cut(s) 885, 935
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 5 cut(s) 62, 218, 960, 1358, 1692
TaiI ACGT 3 cut(s) 12, 140, 1118
TaqI TCGA 3 cut(s) 367, 835, 1687
TasI AATT 9 cut(s) 154, 236, 248, 497, 618, 821, 952, 1320, 1473
TatI WGTACW 1 cut(s) 601
TauI GCSGC 2 cut(s) 1507, 1564
TfiI GAWTC 5 cut(s) 80, 403, 646, 929, 1435
TscAI CASTG 3 cut(s) 238, 1318, 1635
TspDTI ATGAA 7 cut(s) 261, 303, 570, 638, 639, 1053, 1116
TspGWI ACGGA 3 cut(s) 39, 581, 1497
TspRI CASTG 3 cut(s) 238, 1318, 1635
Vha464I CTTAAG 1 cut(s) 275
XapI RAATTY 4 cut(s) 236, 248, 952, 1473
XceI RCATGY 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 366
XmnI GAANNNNTTC 4 cut(s) 248, 445, 780, 810
Zsp2I ATGCAT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.