Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0008113

Physical Location & Seq

Genomic Coordinates Reverse (-)

1036041 .. 1044355

Gene Structure 8315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g01210.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGAATTCTGGAAGTTCCTTCCTGCAGCTCTTAAGCATGTTTATGAAAATGGTCGATCAACAGGGAAAGAAAGCTGCCACAAGACTGGTTGACATATGGGAAGAAAGAAAGGTTTTTGGGTCTCGAGGGCATAGTCTTAAAGATGATATGATGGGAAGGAATCCTCCTGCTCCACCTGCAAGCAATGGAAAAAGTTCAAATCCTATCAAGATAGTGAAGAGGGATGCACACTCAGTTAGACTTAAATTGGCTGTTGGAGGTCTGCCAGAAAAAATACTTACCGCATTTCAACCTGTTCTTGATGCACATCTTACGGAAGAAGCTGCTTTAAACAAGTGTAATGCTGCTGTACTTTATGTGGGTAAAATTGATGAAGACGTTGAAAACACCTTGATTCATGGAACTCAGCAGGGATCCACATTGTTGGATGATTTGAAAGAGCAAGAAGATGCACTTAATCAATCTGTTGGGCAACTTGAGAGCTCGGAGGCAACAAGATCTGCCTTGGTTTTGCAACTCAGAGAAGCACTTCAGGATCAAGAAGCAAAGCTGGAAGTTGTTCGCACTCAATTGCAGGTTGCTCGACATCAGATTGAGAGAGTAAGTAGTATTAAAAGGAGACTGAGTTTGCCCCAGGTTCTGAACCCCGAAAGCAATATTGTTAACATGACACCTGAATCTACCAGGGGGTTAGAACCAAATTTACCATTAGTCCAACAAACTGGCCTCCCACCTCAGCCTCCAATGCAGCCTATGGTTTCTTTTGCCCCTGTAAAAACTACCGATGAAGAGAACAAGAAAGCAGCAGCGGCTGCTGTGGCTGCTAAGCTAGCAGCATCTACCTCTTCTGCACAGATGTTAACGTCTGTTCTTTCATCTCTTGTTGCTGAAGAAGCTGCCTCTATGACTGGTAGCCTGAAATCAGCTGGTTTTACGTCAGGATTATCCATGTTTCCTCCAGAAAAACGGCCCAAGCTTGAGAAACAAATGTCTGATGGTAACAATCCTGATGGTGGCAATGCAGCCTTTTTTACGCCTCTCCAACAGCAGGCAATGACCAGTGTTCCAATCGCGTCATCAGCAACCATGCAGCCTATGTCCCACAGTAACCAGATGCAAAGTCCTTTTAGTCCGCTGCCACCACCCCTGGCACCATCACCATCTACTGCAACTACACCAGCGAATCAATATGTCCAATCTTCTGGTCTTATGGTGGGTGGAATTCCTTACGGATATGGATCAAATACCTTACCGCCTCCACCGCCTATACCTCCACATATTTCAATGAGTATGTCAAGGCCCAGTCCGCCGCAACAGCAGTCACAGCCTCAGCAGCAGCAGCAGTTGCAGCCGGCTGCTGGGGGTTATTATCGGCCACTGGGTATGGGGTTCTACGGGCAAAGCAATCAGTCAAATACGCAGCCAGTAGTACCTCGACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CTD_bind

Protein Analysis

481

Amino Acids

51.54

Weight (kDa)

8.46

Isoelectric Point (pI)

65.57

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008113)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g29400 FvH4_1g29400 FvH4_1g29400
malus_domestica	MD12G1014900.v1.1 MD14G1012500.v1.1
prunus_persica	Prupe.7G025900_v2.0.a1 Prupe.7G025900_v2.0.a1
pyrus_communis	pycom12g01210 pycom14g01070
rosa_chinensis	RchiOBHm_Chr3g0495551
rosa_laevigata	RLG00000022610 RLG00000022611 RLG00000022612
rosa_multiflora	Rmu_sc0004755.1_g000001 Rmu_sc0006163.1_g000001 Rmu_sc0014579.1_g000001
rosa_roxburghii	Rroxscaffold_6G00390310
rosa_rugosa	Rorug03G0274000 Rorug03G0274100
rosa_samantha	Rh3AG321900 Rh3BG357000 Rh3CG353800 Rh3DG356600
rosa_wichuraiana	Rw3G028130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	184
Acc36I	ACCTGC	2 cut(s)	184, 565
AccB1I	GGYRCC	1 cut(s)	1150
AccII	CGCG	1 cut(s)	1073
AciI	CCGC	7 cut(s)	282, 809, 1133, 1253, 1262, 1307, 1310
AclWI	GGATC	4 cut(s)	408, 421, 543, 1246
AcoI	YGGCCR	1 cut(s)	1373
AcsI	RAATTY	3 cut(s)	4, 700, 1221
AcuI	CTGAAG	2 cut(s)	515, 909
AfaI	GTAC	2 cut(s)	351, 1431
AfiI	CCNNNNNNNGG	3 cut(s)	1013, 1048, 1358
AflII	CTTAAG	1 cut(s)	31
AgsI	TTSAA	5 cut(s)	198, 290, 383, 436, 1284
AjnI	CCWGG	3 cut(s)	633, 683, 1146
AluBI	AGCT	9 cut(s)	28, 74, 323, 483, 550, 829, 896, 926, 976
AluI	AGCT	9 cut(s)	28, 74, 323, 483, 550, 829, 896, 926, 976
Alw21I	GWGCWC	1 cut(s)	485
Alw26I	GTCTC	2 cut(s)	126, 613
AlwI	GGATC	4 cut(s)	408, 421, 543, 1246
AlwNI	CAGNNNCTG	2 cut(s)	640, 812
Ama87I	CYCGRG	1 cut(s)	123
AoxI	GGCC	4 cut(s)	724, 968, 1298, 1373
ApoI	RAATTY	3 cut(s)	4, 700, 1221
Asp700I	GAANNNNTTC	3 cut(s)	193, 528, 558
AspS9I	GGNCC	2 cut(s)	969, 1299
AsuHPI	GGTGA	1 cut(s)	1149
AsuNHI	GCTAGC	1 cut(s)	829
AvaI	CYCGRG	1 cut(s)	123
BamHI	GGATCC	1 cut(s)	413
BanI	GGYRCC	1 cut(s)	1150
BanII	GRGCYC	1 cut(s)	485
BbsI	GAAGAC	1 cut(s)	381
Bbv12I	GWGCWC	1 cut(s)	485
BbvCI	CCTCAGC	2 cut(s)	735, 1329
BccI	CCATC	5 cut(s)	145, 989, 1004, 1162, 1168
BceAI	ACGGC	1 cut(s)	983
BcgI	CGANNNNNNTGC	2 cut(s)	563, 597
BciT130I	CCWGG	3 cut(s)	635, 685, 1148
BcoDI	GTCTC	2 cut(s)	126, 613
BfaI	CTAG	1 cut(s)	830
BfmI	CTRYAG	1 cut(s)	23
BfrI	CTTAAG	1 cut(s)	31
BfuAI	ACCTGC	2 cut(s)	184, 565
BglII	AGATCT	1 cut(s)	497
BlpI	GCTNAGC	1 cut(s)	825
Bme1390I	CCNGG	3 cut(s)	635, 685, 1148
BmeT110I	CYCGRG	1 cut(s)	123
BmgT120I	GGNCC	2 cut(s)	969, 1299
BmiI	GGNNCC	2 cut(s)	415, 1152
BmrFI	CCNGG	3 cut(s)	635, 685, 1148
BmrI	ACTGGG	2 cut(s)	1296, 1388
BmsI	GCATC	5 cut(s)	214, 292, 439, 845, 1104
BmtI	GCTAGC	1 cut(s)	833
BmuI	ACTGGG	2 cut(s)	1296, 1388
BpiI	GAAGAC	1 cut(s)	381
BpmI	CTGGAG	1 cut(s)	942
Bpu10I	CCTNAGC	2 cut(s)	735, 1329
Bpu1102I	GCTNAGC	1 cut(s)	825
BpuEI	CTTGAG	2 cut(s)	497, 998
BsaBI	GATNNNNATC	2 cut(s)	306, 1237
BsaI	GGTCTC	1 cut(s)	126
BsaJI	CCNNGG	4 cut(s)	504, 633, 684, 1146
BsaXI	ACNNNNNCTCC	2 cut(s)	610, 640
Bsc4I	CCNNNNNNNGG	3 cut(s)	1013, 1048, 1358
Bse118I	RCCGGY	1 cut(s)	1351
Bse1I	ACTGG	7 cut(s)	90, 727, 913, 1059, 1302, 1383, 1424
Bse3DI	GCAATG	3 cut(s)	190, 1024, 1059
Bse8I	GATNNNNATC	2 cut(s)	306, 1237
BseBI	CCWGG	3 cut(s)	635, 685, 1148
BseDI	CCNNGG	4 cut(s)	504, 633, 684, 1146
BseGI	GGATG	2 cut(s)	229, 433
BseJI	GATNNNNATC	2 cut(s)	306, 1237
BseLI	CCNNNNNNNGG	3 cut(s)	1013, 1048, 1358
BseMI	GCAATG	3 cut(s)	190, 1024, 1059
BseMII	CTCAG	6 cut(s)	246, 419, 532, 614, 749, 1343
BseNI	ACTGG	7 cut(s)	90, 727, 913, 1059, 1302, 1383, 1424
BseYI	CCCAGC	1 cut(s)	1358
BsgI	GTGCAG	1 cut(s)	834
Bsh1236I	CGCG	1 cut(s)	1073
BshFI	GGCC	4 cut(s)	726, 970, 1300, 1375
BshNI	GGYRCC	1 cut(s)	1150
BsiHKAI	GWGCWC	1 cut(s)	485
BsiHKCI	CYCGRG	1 cut(s)	123
BsiSI	CCGG	1 cut(s)	1352
BslFI	GGGAC	1 cut(s)	1084
BslI	CCNNNNNNNGG	3 cut(s)	1013, 1048, 1358
BsmAI	GTCTC	2 cut(s)	126, 613
BsmFI	GGGAC	1 cut(s)	1084
BsnI	GGCC	4 cut(s)	726, 970, 1300, 1375
Bso31I	GGTCTC	1 cut(s)	126
BsoBI	CYCGRG	1 cut(s)	123
Bsp1286I	GDGCHC	1 cut(s)	485
Bsp143I	GATC	5 cut(s)	55, 413, 497, 535, 1238
Bsp1720I	GCTNAGC	1 cut(s)	825
BspACI	CCGC	7 cut(s)	282, 809, 1133, 1253, 1262, 1307, 1310
BspANI	GGCC	4 cut(s)	726, 970, 1300, 1375
BspCNI	CTCAG	6 cut(s)	245, 418, 531, 615, 748, 1342
BspFNI	CGCG	1 cut(s)	1073
BspLI	GGNNCC	2 cut(s)	415, 1152
BspMAI	CTGCAG	1 cut(s)	27
BspMI	ACCTGC	2 cut(s)	184, 565
BspOI	GCTAGC	1 cut(s)	833
BspPI	GGATC	4 cut(s)	408, 421, 543, 1246
BspT107I	GGYRCC	1 cut(s)	1150
BspTI	CTTAAG	1 cut(s)	31
BspTNI	GGTCTC	1 cut(s)	126
BsrDI	GCAATG	3 cut(s)	190, 1024, 1059
BsrFI	RCCGGY	1 cut(s)	1351
BsrI	ACTGG	7 cut(s)	90, 727, 913, 1059, 1302, 1383, 1424
BssAI	RCCGGY	1 cut(s)	1351
BssECI	CCNNGG	4 cut(s)	504, 633, 684, 1146
BssMI	GATC	5 cut(s)	55, 413, 497, 535, 1238
BssT1I	CCWWGG	1 cut(s)	504
Bst2UI	CCWGG	3 cut(s)	635, 685, 1148
Bst4CI	ACNGT	2 cut(s)	1106, 1440
Bst6I	CTCTTC	3 cut(s)	212, 783, 850
BstAFI	CTTAAG	1 cut(s)	31
BstAPI	GCANNNNNTGC	2 cut(s)	812, 1345
BstC8I	GCNNGC	4 cut(s)	181, 831, 1050, 1353
BstDEI	CTNAG	7 cut(s)	232, 405, 518, 623, 735, 825, 1329
BstF5I	GGATG	2 cut(s)	229, 433
BstFNI	CGCG	1 cut(s)	1073
BstKTI	GATC	5 cut(s)	58, 416, 500, 538, 1241
BstMAI	GTCTC	2 cut(s)	126, 613
BstMBI	GATC	5 cut(s)	55, 413, 497, 535, 1238
BstNI	CCWGG	3 cut(s)	635, 685, 1148
BstNSI	RCATGY	1 cut(s)	40
BstSCI	CCNGG	3 cut(s)	633, 683, 1146
BstSFI	CTRYAG	1 cut(s)	23
BstUI	CGCG	1 cut(s)	1073
BstV2I	GAAGAC	1 cut(s)	381
BstX2I	RGATCY	2 cut(s)	413, 497
BstXI	CCANNNNNNTGG	4 cut(s)	85, 424, 722, 1202
BstYI	RGATCY	2 cut(s)	413, 497
BsuRI	GGCC	4 cut(s)	726, 970, 1300, 1375
BtsCI	GGATG	2 cut(s)	229, 433
BtsIMutI	CAGTG	2 cut(s)	1066, 1376
BveI	ACCTGC	2 cut(s)	184, 565
Cac8I	GCNNGC	4 cut(s)	181, 831, 1050, 1353
CaiI	CAGNNNCTG	2 cut(s)	640, 812
Cfr10I	RCCGGY	1 cut(s)	1351
Cfr13I	GGNCC	2 cut(s)	969, 1299
CseI	GACGC	1 cut(s)	1062
Csp6I	GTAC	2 cut(s)	350, 1430
CviAII	CATG	5 cut(s)	37, 398, 667, 949, 1087
CviQI	GTAC	2 cut(s)	350, 1430
DdeI	CTNAG	7 cut(s)	232, 405, 518, 623, 735, 825, 1329
DpnI	GATC	5 cut(s)	57, 415, 499, 537, 1240
DpnII	GATC	5 cut(s)	55, 413, 497, 535, 1238
DraI	TTTAAA	1 cut(s)	330
EaeI	YGGCCR	1 cut(s)	1373
Eam1104I	CTCTTC	3 cut(s)	212, 783, 850
EarI	CTCTTC	3 cut(s)	212, 783, 850
EciI	GGCGGA	1 cut(s)	1296
Ecl136II	GAGCTC	1 cut(s)	483
Eco130I	CCWWGG	1 cut(s)	504
Eco24I	GRGCYC	1 cut(s)	485
Eco31I	GGTCTC	1 cut(s)	126
Eco53kI	GAGCTC	1 cut(s)	483
Eco57I	CTGAAG	2 cut(s)	515, 909
Eco88I	CYCGRG	1 cut(s)	123
EcoICRI	GAGCTC	1 cut(s)	483
EcoRI	GAATTC	2 cut(s)	4, 1221
EcoRII	CCWGG	3 cut(s)	633, 683, 1146
EcoT14I	CCWWGG	1 cut(s)	504
EcoT38I	GRGCYC	1 cut(s)	485
ErhI	CCWWGG	1 cut(s)	504
FaeI	CATG	5 cut(s)	40, 401, 670, 952, 1090
FalI	AAGNNNNNCTT	2 cut(s)	438, 470
FaqI	GGGAC	1 cut(s)	1084
FatI	CATG	5 cut(s)	36, 397, 666, 948, 1086
FauNDI	CATATG	1 cut(s)	95
FokI	GGATG	2 cut(s)	236, 440
FriOI	GRGCYC	1 cut(s)	485
FspBI	CTAG	1 cut(s)	830
GsaI	CCCAGC	1 cut(s)	1362
GsuI	CTGGAG	1 cut(s)	942
HaeIII	GGCC	4 cut(s)	726, 970, 1300, 1375
HapII	CCGG	1 cut(s)	1352
HgaI	GACGC	1 cut(s)	1062
Hin1II	CATG	5 cut(s)	40, 401, 670, 952, 1090
HincII	GTYRAC	3 cut(s)	91, 664, 861
HindII	GTYRAC	3 cut(s)	91, 664, 861
HindIII	AAGCTT	1 cut(s)	974
HinfI	GANTC	4 cut(s)	160, 394, 677, 1183
HpaI	GTTAAC	2 cut(s)	664, 861
HpaII	CCGG	1 cut(s)	1352
HphI	GGTGA	1 cut(s)	1149
Hpy166II	GTNNAC	3 cut(s)	91, 664, 861
Hpy188I	TCNGA	5 cut(s)	487, 521, 591, 642, 994
Hpy188III	TCNNGA	9 cut(s)	9, 123, 208, 299, 533, 539, 939, 959, 1007
Hpy8I	GTNNAC	3 cut(s)	91, 664, 861
HpyAV	CCTTC	2 cut(s)	28, 150
HpyCH4III	ACNGT	2 cut(s)	1106, 1440
HpyCH4IV	ACGT	3 cut(s)	378, 863, 935
HpyF3I	CTNAG	7 cut(s)	232, 405, 518, 623, 735, 825, 1329
HpySE526I	ACGT	3 cut(s)	378, 863, 935
Hsp92II	CATG	5 cut(s)	40, 401, 670, 952, 1090
KroI	GCCGGC	1 cut(s)	1351
KroNI	GCCGGC	1 cut(s)	1353
KspAI	GTTAAC	2 cut(s)	664, 861
Kzo9I	GATC	5 cut(s)	55, 413, 497, 535, 1238
LmnI	GCTCC	1 cut(s)	175
LweI	GCATC	5 cut(s)	214, 292, 439, 845, 1104
MaeI	CTAG	1 cut(s)	830
MaeII	ACGT	3 cut(s)	378, 863, 935
MaeIII	GTNAC	3 cut(s)	998, 1106, 1320
MalI	GATC	5 cut(s)	57, 415, 499, 537, 1240
MboI	GATC	5 cut(s)	55, 413, 497, 535, 1238
MboII	GAAGA	9 cut(s)	113, 229, 329, 386, 458, 800, 837, 902, 1191
MfeI	CAATTG	1 cut(s)	569
MflI	RGATCY	2 cut(s)	413, 497
MhlI	GDGCHC	1 cut(s)	485
MluCI	AATT	6 cut(s)	4, 245, 366, 569, 700, 1221
MmeI	TCCRAC	4 cut(s)	235, 405, 739, 1066
MroNI	GCCGGC	1 cut(s)	1351
MroXI	GAANNNNTTC	3 cut(s)	193, 528, 558
MseI	TTAA	8 cut(s)	32, 138, 243, 329, 456, 612, 663, 860
MslI	CAYNNNNRTG	1 cut(s)	41
MspA1I	CMGCKG	3 cut(s)	809, 926, 1135
MspCI	CTTAAG	1 cut(s)	31
MspI	CCGG	1 cut(s)	1352
MspR9I	CCNGG	3 cut(s)	635, 685, 1148
MunI	CAATTG	1 cut(s)	569
MvaI	CCWGG	3 cut(s)	635, 685, 1148
MvnI	CGCG	1 cut(s)	1073
NaeI	GCCGGC	1 cut(s)	1353
NdeI	CATATG	1 cut(s)	95
NdeII	GATC	5 cut(s)	55, 413, 497, 535, 1238
NgoMIV	GCCGGC	1 cut(s)	1351
NheI	GCTAGC	1 cut(s)	829
NlaIII	CATG	5 cut(s)	40, 401, 670, 952, 1090
NlaIV	GGNNCC	2 cut(s)	415, 1152
NmuCI	GTSAC	1 cut(s)	1320
NspI	RCATGY	1 cut(s)	40
PaeR7I	CTCGAG	1 cut(s)	123
PaqCI	CACCTGC	1 cut(s)	184
PdiI	GCCGGC	1 cut(s)	1353
PdmI	GAANNNNTTC	3 cut(s)	193, 528, 558
PfeI	GAWTC	4 cut(s)	160, 394, 677, 1183
Psp124BI	GAGCTC	1 cut(s)	485
Psp6I	CCWGG	3 cut(s)	633, 683, 1146
PspFI	CCCAGC	1 cut(s)	1358
PspGI	CCWGG	3 cut(s)	633, 683, 1146
PspN4I	GGNNCC	2 cut(s)	415, 1152
PspPI	GGNCC	2 cut(s)	969, 1299
PsrI	GAACNNNNNNTAC	2 cut(s)	687, 719
PstI	CTGCAG	1 cut(s)	27
PstNI	CAGNNNCTG	2 cut(s)	640, 812
PsuI	RGATCY	2 cut(s)	413, 497
PvuII	CAGCTG	1 cut(s)	926
RsaI	GTAC	2 cut(s)	351, 1431
RsaNI	GTAC	2 cut(s)	350, 1430
RseI	CAYNNNNRTG	1 cut(s)	41
SacI	GAGCTC	1 cut(s)	485
SaqAI	TTAA	8 cut(s)	32, 138, 243, 329, 456, 612, 663, 860
Sau3AI	GATC	5 cut(s)	55, 413, 497, 535, 1238
Sau96I	GGNCC	2 cut(s)	969, 1299
ScrFI	CCNGG	3 cut(s)	635, 685, 1148
SduI	GDGCHC	1 cut(s)	485
SfaNI	GCATC	5 cut(s)	214, 292, 439, 845, 1104
SfcI	CTRYAG	1 cut(s)	23
Sfr274I	CTCGAG	1 cut(s)	123
SlaI	CTCGAG	1 cut(s)	123
SmiMI	CAYNNNNRTG	1 cut(s)	41
SmlI	CTYRAG	4 cut(s)	31, 123, 476, 977
SmoI	CTYRAG	4 cut(s)	31, 123, 476, 977
Sse9I	AATT	6 cut(s)	4, 245, 366, 569, 700, 1221
SsiI	CCGC	7 cut(s)	282, 809, 1133, 1253, 1262, 1307, 1310
SspI	AATATT	1 cut(s)	658
SspMI	CTAG	1 cut(s)	830
SstI	GAGCTC	1 cut(s)	485
StyD4I	CCNGG	3 cut(s)	633, 683, 1146
StyI	CCWWGG	1 cut(s)	504
TaaI	ACNGT	2 cut(s)	1106, 1440
TaiI	ACGT	3 cut(s)	381, 866, 938
TaqI	TCGA	4 cut(s)	54, 124, 583, 1435
TasI	AATT	6 cut(s)	4, 245, 366, 569, 700, 1221
TatI	WGTACW	1 cut(s)	349
TauI	GCSGC	2 cut(s)	812, 1312
TfiI	GAWTC	4 cut(s)	160, 394, 677, 1183
Tru1I	TTAA	8 cut(s)	32, 138, 243, 329, 456, 612, 663, 860
Tru9I	TTAA	8 cut(s)	32, 138, 243, 329, 456, 612, 663, 860
TscAI	CASTG	2 cut(s)	1066, 1383
TseFI	GTSAC	1 cut(s)	1320
Tsp45I	GTSAC	1 cut(s)	1320
TspDTI	ATGAA	6 cut(s)	17, 59, 386, 387, 801, 864
TspGWI	ACGGA	2 cut(s)	329, 1245
TspRI	CASTG	2 cut(s)	1066, 1383
Vha464I	CTTAAG	1 cut(s)	31
XapI	RAATTY	3 cut(s)	4, 700, 1221
XceI	RCATGY	1 cut(s)	40
XhoI	CTCGAG	1 cut(s)	123
XmnI	GAANNNNTTC	3 cut(s)	193, 528, 558
XspI	CTAG	1 cut(s)	830

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.