Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007468

Physical Location & Seq

Genomic Coordinates Reverse (-)

3226061 .. 3230741

Gene Structure 4681 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1029600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1206 bp

ATGATTACCACGCAGAGAAGCATATCGGGGCCGTCGCAGAGCCCTAGATCTCCGTCGTCGCAGCCGTACCTCTCAGTCTCAGTGACTGATCCTGTGAAATTGGGAAATGGCGTCCAGGCATACATCTCCTACCGAGTGATCACCAAGACAAATTTTCCTGAATACCAAGGGCCGGAGAAGATTGTTATTCGACGTTACAGTGATTTTGTTTGGCTACGGGATCGTCTCTTTGAAAAGTATAAAGGAATTTTTATTCCACCTCTTCCAGAAAAGAGTGCTGTAGAAAAGTTCCGTTTCAGTGCTGAATTTATTGAGATGAGGCGTCAAGCATTGGATGTATTTGTTAATCGGATAGCTTCACATCATGAACTTCAGCAAAGTGAGGATCTGAGAACCTTCTTACAAGCAGATGAAGAGACAATGGAAAGGTTAAGGTTTCACGAGACTGGTATCTTTAAGAAGAAGCCAGCAGATTTGATGCAAATCTTCAAGGATGTACAATCTAAAGTGAGCGATGTTGTTCTCGGAAAGGAAAAGCCGGTGGAAGAGTCAAATCCTGAATATGAGAAGTTGAAACACTACATCTTTGAGCTTGAAAACCACTTGGCTGAAGCCCAGAAGCATGCATACCGTCTTGTCAAGAGGCACAGAGAGCTGGGACAGTCTCTAGCAGATTTTGGGAAAGCAGCCAAGCTCCTAGGAGCTTCCGAAGGTAATGCTCTTGGAAAGGCCTTTACCGAGCTTGGGGCGAAGTCAGAGACATTATCAATTAGGCTGCAAAAGGAGGCCCAACAACTGTTAATGAATTTTGAAGAACCGTTGAAAGATTATGTCCGTGCCGTGCAATCTATTAAGGCGACAATAGCTGAGAGAGCAAATGCCTTCAGGCAACAGTGTGAACTTGCCGAAACAATTAAGTTGAAGGAGATAAATCTTGACAAACTCATGTTGACCAGATCGGACAGGGTGGGCGAAGCTGAGCACGAGTACAAGGAGTTGAAGGCTGAGGGTGAGGAAGCAACCAGAAGATTTGAAACGATAGTGCGACGGATGAATGAAGAGATAGTTCACTTTCAAGAACAAAAAACAACGGACATGGGGGTTGCTTTCCATGAGTTTGCCAAGGGACAGGCACGCCTGGCAAAAAGTATTGCAGACGCTTGGCGAAGTCTCCTCCCTAAGCTCGAATCTTGCTCCCCGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005794 GO:0005802 GO:0005829 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006892 GO:0006896 GO:0006897 GO:0006996 GO:0007034 GO:0007041 GO:0007275 GO:0008104 GO:0008150 GO:0008289 GO:0008333 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009958 GO:0009987 GO:0010008 GO:0010252 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019898 GO:0022622 GO:0030904 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032588 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0035091 GO:0042592 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098588 GO:0098657 GO:0098791 GO:0098796 GO:0098805 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

PX Vps5

Protein Analysis

402

Amino Acids

46.13

Weight (kDa)

8.55

Isoelectric Point (pI)

44.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PX	PF00787	58 - 136	1.4e-22	PX domain
Vps5	PF09325	166 - 393	1.5e-23	Vps5 C terminal like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007468)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06140
fragaria_vesca	FvH4_6g19390 FvH4_6g19390 FvH4_6g19390
malus_domestica	MD12G1029600.v1.1 MD14G1029900.v1.1
prunus_persica	Prupe.7G102200_v2.0.a1 Prupe.7G102200_v2.0.a1 Prupe.7G102200_v2.0.a1
pyrus_communis	pycom12g02800 pycom14g02770
rosa_chinensis	RchiOBHm_Chr3g0474681
rosa_laevigata	RLG00000023902
rosa_multiflora	Rmu_sc0001126.1_g000005
rosa_roxburghii	Rroxscaffold_6G00406980
rosa_rugosa	Rorug03G0142300.1 Rorug03G0142400 Rorug03G0142500.1 Rorug03G0142600 Rorug03G0142700
rosa_samantha	Rh3AG192900 Rh3BG222800 Rh3CG217600 Rh3DG218700
rosa_wichuraiana	Rw3G017590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	3 cut(s)	83, 228, 393
AcsI	RAATTY	4 cut(s)	151, 246, 305, 805
AcuI	CTGAAG	3 cut(s)	356, 630, 868
AcyI	GRCGYC	2 cut(s)	111, 322
AfaI	GTAC	3 cut(s)	68, 498, 989
AfiI	CCNNNNNNNGG	2 cut(s)	172, 744
AjnI	CCWGG	2 cut(s)	114, 1137
AluBI	AGCT	9 cut(s)	356, 592, 655, 694, 704, 742, 866, 977, 1183
AluI	AGCT	9 cut(s)	356, 592, 655, 694, 704, 742, 866, 977, 1183
Alw21I	GWGCWC	1 cut(s)	984
Alw26I	GTCTC	7 cut(s)	82, 230, 410, 437, 669, 752, 1175
AlwI	GGATC	3 cut(s)	83, 228, 393
AlwNI	CAGNNNCTG	1 cut(s)	86
AoxI	GGCC	4 cut(s)	29, 170, 729, 786
ApeKI	GCWGC	3 cut(s)	61, 686, 775
ApoI	RAATTY	4 cut(s)	151, 246, 305, 805
AspA2I	CCTAGG	1 cut(s)	697
AspS9I	GGNCC	3 cut(s)	29, 170, 787
AsuC2I	CCSGG	1 cut(s)	1199
AsuHPI	GGTGA	2 cut(s)	133, 1022
AvrII	CCTAGG	1 cut(s)	697
BanII	GRGCYC	1 cut(s)	44
BarI	GAAGNNNNNNTAC	2 cut(s)	611, 643
BauI	CACGAG	2 cut(s)	440, 983
Bbv12I	GWGCWC	1 cut(s)	984
BbvCI	CCTCAGC	1 cut(s)	1005
BbvI	GCAGC	3 cut(s)	73, 698, 762
BceAI	ACGGC	3 cut(s)	16, 49, 824
BcgI	CGANNNNNNTGC	2 cut(s)	698, 732
BciT130I	CCWGG	2 cut(s)	116, 1139
BclI	TGATCA	1 cut(s)	138
BcnI	CCSGG	1 cut(s)	1199
BcoDI	GTCTC	7 cut(s)	82, 230, 410, 437, 669, 752, 1175
BfaI	CTAG	3 cut(s)	45, 668, 698
BfmI	CTRYAG	1 cut(s)	279
BglII	AGATCT	1 cut(s)	47
BisI	GCNGC	3 cut(s)	62, 687, 776
BlnI	CCTAGG	1 cut(s)	697
BlpI	GCTNAGC	1 cut(s)	978
BlsI	GCNGC	3 cut(s)	63, 688, 777
Bme1390I	CCNGG	3 cut(s)	116, 1139, 1199
BmgT120I	GGNCC	3 cut(s)	29, 170, 787
BmiI	GGNNCC	1 cut(s)	30
BmrFI	CCNGG	3 cut(s)	116, 1139, 1199
BmsI	GCATC	1 cut(s)	468
Bpu10I	CCTNAGC	2 cut(s)	1005, 1179
Bpu1102I	GCTNAGC	1 cut(s)	978
BpuMI	CCSGG	1 cut(s)	1199
BsaBI	GATNNNNATC	1 cut(s)	482
BsaHI	GRCGYC	2 cut(s)	111, 322
BsaJI	CCNNGG	4 cut(s)	166, 697, 1122, 1197
Bsc4I	CCNNNNNNNGG	2 cut(s)	172, 744
Bse118I	RCCGGY	1 cut(s)	538
Bse1I	ACTGG	1 cut(s)	451
Bse8I	GATNNNNATC	1 cut(s)	482
BseBI	CCWGG	2 cut(s)	116, 1139
BseDI	CCNNGG	4 cut(s)	166, 697, 1122, 1197
BseGI	GGATG	3 cut(s)	340, 499, 1056
BseJI	GATNNNNATC	1 cut(s)	482
BseLI	CCNNNNNNNGG	2 cut(s)	172, 744
BseMII	CTCAG	6 cut(s)	87, 93, 380, 858, 969, 996
BseNI	ACTGG	1 cut(s)	451
BseRI	GAGGAG	1 cut(s)	1163
BseXI	GCAGC	3 cut(s)	73, 698, 762
BseYI	CCCAGC	1 cut(s)	655
BshFI	GGCC	4 cut(s)	31, 172, 731, 788
BsiHKAI	GWGCWC	1 cut(s)	984
BsiSI	CCGG	3 cut(s)	173, 539, 1199
BslFI	GGGAC	2 cut(s)	672, 1140
BslI	CCNNNNNNNGG	2 cut(s)	172, 744
BsmAI	GTCTC	7 cut(s)	82, 230, 410, 437, 669, 752, 1175
BsmBI	CGTCTC	1 cut(s)	230
BsmFI	GGGAC	2 cut(s)	672, 1140
BsnI	GGCC	4 cut(s)	31, 172, 731, 788
Bsp1286I	GDGCHC	2 cut(s)	44, 984
Bsp1407I	TGTACA	1 cut(s)	496
Bsp143I	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
Bsp1720I	GCTNAGC	1 cut(s)	978
BspANI	GGCC	4 cut(s)	31, 172, 731, 788
BspCNI	CTCAG	6 cut(s)	86, 92, 381, 859, 970, 997
BspHI	TCATGA	1 cut(s)	364
BspLI	GGNNCC	1 cut(s)	30
BspPI	GGATC	3 cut(s)	83, 228, 393
BsrFI	RCCGGY	1 cut(s)	538
BsrGI	TGTACA	1 cut(s)	496
BsrI	ACTGG	1 cut(s)	451
BssAI	RCCGGY	1 cut(s)	538
BssECI	CCNNGG	4 cut(s)	166, 697, 1122, 1197
BssMI	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
BssNI	GRCGYC	2 cut(s)	111, 322
BssSI	CACGAG	2 cut(s)	440, 983
BssT1I	CCWWGG	3 cut(s)	166, 697, 1122
Bst2BI	CACGAG	2 cut(s)	440, 983
Bst2UI	CCWGG	2 cut(s)	116, 1139
Bst4CI	ACNGT	6 cut(s)	200, 632, 663, 798, 819, 894
Bst6I	CTCTTC	4 cut(s)	267, 408, 540, 1053
BstACI	GRCGYC	2 cut(s)	111, 322
BstAUI	TGTACA	1 cut(s)	496
BstC8I	GCNNGC	3 cut(s)	468, 624, 1135
BstDEI	CTNAG	8 cut(s)	73, 79, 389, 867, 978, 1005, 1179, 1203
BstF5I	GGATG	3 cut(s)	340, 499, 1056
BstKTI	GATC	6 cut(s)	50, 91, 141, 223, 388, 959
BstMAI	GTCTC	7 cut(s)	82, 230, 410, 437, 669, 752, 1175
BstMBI	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
BstMWI	GCNNNNNNNGC	4 cut(s)	652, 863, 872, 1139
BstNI	CCWGG	2 cut(s)	116, 1139
BstNSI	RCATGY	1 cut(s)	626
BstSCI	CCNGG	3 cut(s)	114, 1137, 1197
BstSFI	CTRYAG	1 cut(s)	279
BstV1I	GCAGC	3 cut(s)	73, 698, 762
BstX2I	RGATCY	2 cut(s)	47, 385
BstYI	RGATCY	2 cut(s)	47, 385
BsuRI	GGCC	4 cut(s)	31, 172, 731, 788
BtgZI	GCGATG	1 cut(s)	528
BtsCI	GGATG	3 cut(s)	340, 499, 1056
BtsIMutI	CAGTG	4 cut(s)	87, 205, 304, 899
Cac8I	GCNNGC	3 cut(s)	468, 624, 1135
CaiI	CAGNNNCTG	1 cut(s)	86
CciI	TCATGA	1 cut(s)	364
Cfr10I	RCCGGY	1 cut(s)	538
Cfr13I	GGNCC	3 cut(s)	29, 170, 787
CseI	GACGC	3 cut(s)	100, 311, 1166
Csp6I	GTAC	3 cut(s)	67, 497, 988
CviAII	CATG	5 cut(s)	365, 623, 946, 1096, 1112
CviQI	GTAC	3 cut(s)	67, 497, 988
DdeI	CTNAG	8 cut(s)	73, 79, 389, 867, 978, 1005, 1179, 1203
DpnI	GATC	6 cut(s)	49, 90, 140, 222, 387, 958
DpnII	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
Eam1104I	CTCTTC	4 cut(s)	267, 408, 540, 1053
EarI	CTCTTC	4 cut(s)	267, 408, 540, 1053
Eco130I	CCWWGG	3 cut(s)	166, 697, 1122
Eco147I	AGGCCT	1 cut(s)	731
Eco24I	GRGCYC	1 cut(s)	44
Eco57I	CTGAAG	3 cut(s)	356, 630, 868
EcoRII	CCWGG	2 cut(s)	114, 1137
EcoT14I	CCWWGG	3 cut(s)	166, 697, 1122
EcoT22I	ATGCAT	1 cut(s)	628
EcoT38I	GRGCYC	1 cut(s)	44
ErhI	CCWWGG	3 cut(s)	166, 697, 1122
Esp3I	CGTCTC	1 cut(s)	230
FaeI	CATG	5 cut(s)	368, 626, 949, 1099, 1115
FaqI	GGGAC	2 cut(s)	672, 1140
FatI	CATG	5 cut(s)	364, 622, 945, 1095, 1111
FbaI	TGATCA	1 cut(s)	138
Fnu4HI	GCNGC	3 cut(s)	62, 687, 776
FokI	GGATG	3 cut(s)	347, 506, 1063
FriOI	GRGCYC	1 cut(s)	44
Fsp4HI	GCNGC	3 cut(s)	62, 687, 776
FspBI	CTAG	3 cut(s)	45, 668, 698
GluI	GCNGC	3 cut(s)	62, 687, 776
GsaI	CCCAGC	1 cut(s)	659
HaeIII	GGCC	4 cut(s)	31, 172, 731, 788
HapII	CCGG	3 cut(s)	173, 539, 1199
HgaI	GACGC	3 cut(s)	100, 311, 1166
Hin1I	GRCGYC	2 cut(s)	111, 322
Hin1II	CATG	5 cut(s)	368, 626, 949, 1099, 1115
HincII	GTYRAC	1 cut(s)	951
HindII	GTYRAC	1 cut(s)	951
HinfI	GANTC	2 cut(s)	548, 1187
HpaII	CCGG	3 cut(s)	173, 539, 1199
HphI	GGTGA	2 cut(s)	133, 1022
Hpy166II	GTNNAC	3 cut(s)	899, 951, 1069
Hpy188I	TCNGA	6 cut(s)	351, 390, 527, 709, 757, 961
Hpy188III	TCNNGA	8 cut(s)	158, 266, 365, 440, 557, 640, 935, 1076
Hpy8I	GTNNAC	3 cut(s)	899, 951, 1069
Hpy99I	CGWCG	5 cut(s)	37, 58, 61, 195, 1050
HpyAV	CCTTC	5 cut(s)	406, 704, 892, 916, 994
HpyCH4III	ACNGT	6 cut(s)	200, 632, 663, 798, 819, 894
HpyCH4IV	ACGT	1 cut(s)	193
HpyCH4V	TGCA	5 cut(s)	481, 626, 778, 844, 1154
HpyF10VI	GCNNNNNNNGC	4 cut(s)	652, 863, 872, 1139
HpyF3I	CTNAG	8 cut(s)	73, 79, 389, 867, 978, 1005, 1179, 1203
HpySE526I	ACGT	1 cut(s)	193
Hsp92I	GRCGYC	2 cut(s)	111, 322
Hsp92II	CATG	5 cut(s)	368, 626, 949, 1099, 1115
Ksp22I	TGATCA	1 cut(s)	138
Kzo9I	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
LmnI	GCTCC	3 cut(s)	699, 701, 1199
Lsp1109I	GCAGC	3 cut(s)	73, 698, 762
LweI	GCATC	1 cut(s)	468
MaeI	CTAG	3 cut(s)	45, 668, 698
MaeII	ACGT	1 cut(s)	193
MaeIII	GTNAC	2 cut(s)	82, 194
MalI	GATC	6 cut(s)	49, 90, 140, 222, 387, 958
MboI	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
MboII	GAAGA	9 cut(s)	190, 254, 425, 472, 478, 557, 824, 1038, 1070
MflI	RGATCY	2 cut(s)	47, 385
MhlI	GDGCHC	2 cut(s)	44, 984
MluCI	AATT	7 cut(s)	98, 151, 246, 305, 768, 805, 912
MlyI	GAGTC	1 cut(s)	557
MnlI	CCTC	9 cut(s)	80, 270, 312, 376, 636, 778, 1000, 1006, 1184
Mph1103I	ATGCAT	1 cut(s)	628
MseI	TTAA	6 cut(s)	345, 431, 456, 800, 852, 915
MspI	CCGG	3 cut(s)	173, 539, 1199
MspR9I	CCNGG	3 cut(s)	116, 1139, 1199
MvaI	CCWGG	2 cut(s)	116, 1139
MwoI	GCNNNNNNNGC	4 cut(s)	652, 863, 872, 1139
NciI	CCSGG	1 cut(s)	1199
NdeII	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
NlaIII	CATG	5 cut(s)	368, 626, 949, 1099, 1115
NlaIV	GGNNCC	1 cut(s)	30
NmuCI	GTSAC	1 cut(s)	82
NsiI	ATGCAT	1 cut(s)	628
NspI	RCATGY	1 cut(s)	626
PaeI	GCATGC	1 cut(s)	626
PagI	TCATGA	1 cut(s)	364
PceI	AGGCCT	1 cut(s)	731
PcsI	WCGNNNNNNNCGW	1 cut(s)	62
PfeI	GAWTC	1 cut(s)	1187
PkrI	GCNGC	3 cut(s)	63, 688, 777
PleI	GAGTC	1 cut(s)	556
PpsI	GAGTC	1 cut(s)	556
Psp6I	CCWGG	2 cut(s)	114, 1137
PspFI	CCCAGC	1 cut(s)	655
PspGI	CCWGG	2 cut(s)	114, 1137
PspN4I	GGNNCC	1 cut(s)	30
PspPI	GGNCC	3 cut(s)	29, 170, 787
PstNI	CAGNNNCTG	1 cut(s)	86
PsuI	RGATCY	2 cut(s)	47, 385
RsaI	GTAC	3 cut(s)	68, 498, 989
RsaNI	GTAC	3 cut(s)	67, 497, 988
SaqAI	TTAA	6 cut(s)	345, 431, 456, 800, 852, 915
SatI	GCNGC	3 cut(s)	62, 687, 776
Sau3AI	GATC	6 cut(s)	47, 88, 138, 220, 385, 956
Sau96I	GGNCC	3 cut(s)	29, 170, 787
SchI	GAGTC	1 cut(s)	557
ScrFI	CCNGG	3 cut(s)	116, 1139, 1199
SduI	GDGCHC	2 cut(s)	44, 984
SfaNI	GCATC	1 cut(s)	468
SfcI	CTRYAG	1 cut(s)	279
SphI	GCATGC	1 cut(s)	626
Sse9I	AATT	7 cut(s)	98, 151, 246, 305, 768, 805, 912
SseBI	AGGCCT	1 cut(s)	731
SspMI	CTAG	3 cut(s)	45, 668, 698
StuI	AGGCCT	1 cut(s)	731
StyD4I	CCNGG	3 cut(s)	114, 1137, 1197
StyI	CCWWGG	3 cut(s)	166, 697, 1122
TaaI	ACNGT	6 cut(s)	200, 632, 663, 798, 819, 894
TaiI	ACGT	1 cut(s)	196
TaqI	TCGA	2 cut(s)	190, 1185
TasI	AATT	7 cut(s)	98, 151, 246, 305, 768, 805, 912
TatI	WGTACW	2 cut(s)	496, 987
TfiI	GAWTC	1 cut(s)	1187
Tru1I	TTAA	6 cut(s)	345, 431, 456, 800, 852, 915
Tru9I	TTAA	6 cut(s)	345, 431, 456, 800, 852, 915
TscAI	CASTG	4 cut(s)	87, 205, 304, 899
TseFI	GTSAC	1 cut(s)	82
TseI	GCWGC	3 cut(s)	61, 686, 775
Tsp45I	GTSAC	1 cut(s)	82
TspDTI	ATGAA	5 cut(s)	381, 426, 818, 1067, 1071
TspGWI	ACGGA	5 cut(s)	42, 281, 824, 1063, 1106
TspRI	CASTG	4 cut(s)	87, 205, 304, 899
XapI	RAATTY	4 cut(s)	151, 246, 305, 805
XceI	RCATGY	1 cut(s)	626
XmaJI	CCTAGG	1 cut(s)	697
XspI	CTAG	3 cut(s)	45, 668, 698
Zsp2I	ATGCAT	1 cut(s)	628

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.