Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005886

Physical Location & Seq

Genomic Coordinates Forward (+)

5195688 .. 5202055

Gene Structure 6368 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1046200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGGTTGCTCCACCCGGTTCTCTGCCTCCCACTTCTCTGAAGATTGGTGGTGGGAATTCAACAGTCAAGAGAAAGACACCATCCCAACTGAGAGGAGAGCAGCGGATGAATGCTGTGGAGATAAGTGATGAATCTCCAGCCCCTTTGCTTGGTTCTACAAATGAGGCAGAAAATGGGCAAAAGAAACAAGAACCGTCAAGGAATCCTAGATACATTGACATTCGTATGGATGAAGTTTACCCTGCAAAAAAATCTAGGTTCAAGATACTCTCTGGAAAAGAGAATGCTAAGGAAAGTAATTCAATGGAGAAACCTAGCAGTGTGAAGAATCTTTCTATGCTTTCTAATTTGGCTTCCAAGAGGAAACACCAACTTTCTGGTCCCCAAAATATAGATTCTGCTGAAAATACTGAAGAAGGTGCGGCAAAAGTTTTTCGAACTATTGAAAAGTGTAGTCAAACTACTTTCCGTAGTGTTACTGAGCTTTCATCAGGCGGTGACAGATTATCGGACTTTGCAGCTGTCGATGTGGATAAAGCGTTAAAAGGAATAGATGCCCAGGAATCTACAATTGGCTCATCCGCTTATATTTCTGAAAGAAGTGGCAATCCTACATCACCTGGTTTCGGCAACTTTTGCTTGCCTGATGGAAAGGTTCCTCTGGATTTTTCTTTGAAAAGTAGTATGCGGGTGGTGTCCTCTTCCCCGGTGAATTGGATTCATAGGACAATAGCATGCGGTGCCTACACTAGTATACCGAATCTCAAGTTCCAATCCGATCATGATCAGGATCAAAATATGAGCCATGGCTCAGGGCTCATATCGAATTCCCAAGTCATTAGTTCTAAACAATTATATTCGTGGGTTTACCCTCAATCTACCTTACCGCCTTCTCTCATACAAGTTTTGACATCATCAACAGTTGAGGGAGTGGAAACAGAATTCTTGAGAAAACGACAGGTAGCATGGGATGACTCATTTCAGAGTCTATACTACATGCTTCGTAATGGTACCTGTGATATCTTTTATGTGTGTACTCCATATTTTGTGGTAATGTTCACGGCTTGTAATGTCTCTGTGGGCTCAAAACGCATCAGCAATGCTTGTATCTCCCAGTCAACAAGAGCCATACGATCATTGTTAAGAGAGCATGATGTTTCTTTCTCTATGCCTCTTTGCCGTTCTAAAGTAGAGCAAGTCACTCCAGATGTTCTTGTTGAGCTCTCAGAGATGGAGAAACAGAATTTTGGCCAGACACGGCGCTTGAGTTCCTCTTCTGATATAGATAATACCCCGGAATCATTGCTAGTTTTCAATGGCAATAAAAATGTACATGGAGTGTTTGATATTTTGTTAAATTATAGGTCGTTGTTGACTGCTTTAATGGTAATGGATGTCCCTGTGTTATATTCACCTGTGCCGTTTCAGAATGCTGCCATTTCTTCTCCAGAGATTAAATGCACGGAATTGAAAAGAGCAGACCATATTGTTGCTCCCAAAAAGACCACCACAAAAGATGATGGATCCATGCAAGGCTCATCCGCTGGTCTCTGCTCGAGTGTTGAAATTAAGGATGCATATCTTCCACCATGGATTATTTGCAGCCTATGTGCCCTTCTGGGTTCTGAAGGGAGAAGCTTTGAGGCGAGCTTTATGAACCAACCTACATCAATCGGGTTGAATGTTGCTGTAGAAGCAGTTCCTGAGAAATCTGATTGTCCTGATGCGCCAGCTGAAGACTTGCACAGAAGCACCTCTACTTTTGGTATTCCAGAAGCCATCGTTACGCCTCACTTGCATTTGGGTTTCTTGAAAACTTTGAAGTTCTCAAATGGTTGTTATTCAGCTTCCCTTTCTCCTACCTTAGAAGCCGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

626

Amino Acids

68.17

Weight (kDa)

6.41

Isoelectric Point (pI)

53.1

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005886)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54750 AT3G54750 AT3G54750 AT3G54750 AT3G54750
fragaria_vesca	FvH4_6g21040 FvH4_6g21040 FvH4_6g21040
malus_domestica	MD12G1046200.v1.1 MD14G1044900.v1.1
prunus_persica	Prupe.7G089700_v2.0.a1 Prupe.7G089700_v2.0.a1
pyrus_communis	pycom12g03970 pycom12g03980 pycom14g03800 pycom14g03810
rosa_chinensis	RchiOBHm_Chr3g0477411
rosa_laevigata	RLG00000023723
rosa_multiflora	Rmu_ssc0000341.1_g000018
rosa_roxburghii	Rroxscaffold_6G00404170 Rroxscaffold_6G00404650
rosa_rugosa	Rorug03G0160600 Rorug03G0160700
rosa_samantha	Rh3AG210900 Rh3BG244300 Rh3CG238100 Rh3DG237800
rosa_wichuraiana	Rw3G019110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1010
AccB1I	GGYRCC	2 cut(s)	740, 1010
AccI	GTMKAC	1 cut(s)	754
AciI	CCGC	8 cut(s)	103, 422, 495, 582, 688, 738, 887, 1542
AclWI	GGATC	3 cut(s)	798, 1518, 1531
AcoI	YGGCCR	1 cut(s)	1249
AcsI	RAATTY	4 cut(s)	55, 826, 941, 1243
AcuI	CTGAAG	4 cut(s)	59, 432, 1647, 1755
AfaI	GTAC	3 cut(s)	1012, 1036, 1332
AfiI	CCNNNNNNNGG	2 cut(s)	149, 626
AhlI	ACTAGT	1 cut(s)	749
AjnI	CCWGG	2 cut(s)	558, 619
AjuI	GAANNNNNNNTTGG	2 cut(s)	555, 587
AluBI	AGCT	7 cut(s)	484, 521, 1222, 1638, 1650, 1733, 1847
AluI	AGCT	7 cut(s)	484, 521, 1222, 1638, 1650, 1733, 1847
Alw21I	GWGCWC	1 cut(s)	1224
Alw26I	GTCTC	2 cut(s)	1078, 1553
AlwI	GGATC	3 cut(s)	798, 1518, 1531
AlwNI	CAGNNNCTG	1 cut(s)	1703
Ama87I	CYCGRG	1 cut(s)	1555
AoxI	GGCC	1 cut(s)	1249
ApeKI	GCWGC	4 cut(s)	100, 518, 1433, 1602
ApoI	RAATTY	4 cut(s)	55, 826, 941, 1243
Asp700I	GAANNNNTTC	1 cut(s)	1698
Asp718I	GGTACC	1 cut(s)	1010
AspLEI	GCGC	2 cut(s)	1263, 1729
AspS9I	GGNCC	1 cut(s)	380
AsuC2I	CCSGG	3 cut(s)	15, 707, 1295
AsuHPI	GGTGA	4 cut(s)	509, 609, 721, 1404
AsuII	TTCGAA	1 cut(s)	436
AvaI	CYCGRG	1 cut(s)	1555
AvaII	GGWCC	1 cut(s)	380
BaeGI	GKGCMC	1 cut(s)	1615
BalI	TGGCCA	1 cut(s)	1251
BamHI	GGATCC	1 cut(s)	1523
BanI	GGYRCC	2 cut(s)	740, 1010
BanII	GRGCYC	3 cut(s)	819, 1085, 1224
BarI	GAAGNNNNNNTAC	2 cut(s)	1741, 1773
BbsI	GAAGAC	1 cut(s)	1743
Bbv12I	GWGCWC	1 cut(s)	1224
BbvI	GCAGC	4 cut(s)	112, 530, 1420, 1614
BccI	CCATC	5 cut(s)	88, 641, 1225, 1514, 1787
BceAI	ACGGC	4 cut(s)	1077, 1164, 1274, 1405
BciT130I	CCWGG	2 cut(s)	560, 621
BclI	TGATCA	1 cut(s)	784
BcnI	CCSGG	3 cut(s)	15, 707, 1295
BcoDI	GTCTC	2 cut(s)	1078, 1553
BcuI	ACTAGT	1 cut(s)	749
BfaI	CTAG	6 cut(s)	207, 255, 315, 750, 1307, 1876
BfmI	CTRYAG	1 cut(s)	1689
BfoI	RGCGCY	1 cut(s)	1264
BisI	GCNGC	5 cut(s)	101, 423, 519, 1434, 1603
BlsI	GCNGC	5 cut(s)	102, 424, 520, 1435, 1604
Bme1390I	CCNGG	5 cut(s)	15, 560, 621, 707, 1295
Bme18I	GGWCC	1 cut(s)	380
BmeT110I	CYCGRG	1 cut(s)	1555
BmgT120I	GGNCC	1 cut(s)	380
BmiI	GGNNCC	5 cut(s)	382, 657, 742, 1012, 1525
BmrFI	CCNGG	5 cut(s)	15, 560, 621, 707, 1295
BmrI	ACTGGG	1 cut(s)	1108
BmsI	GCATC	4 cut(s)	544, 1101, 1564, 1714
BmuI	ACTGGG	1 cut(s)	1108
BpiI	GAAGAC	1 cut(s)	1743
BpmI	CTGGAG	3 cut(s)	120, 1188, 1431
Bpu10I	CCTNAGC	2 cut(s)	288, 811
Bpu14I	TTCGAA	1 cut(s)	436
BpuEI	CTTGAG	3 cut(s)	749, 967, 1285
BpuMI	CCSGG	3 cut(s)	15, 707, 1295
BsaBI	GATNNNNATC	3 cut(s)	783, 789, 1578
BsaI	GGTCTC	1 cut(s)	1553
BsaJI	CCNNGG	5 cut(s)	558, 705, 805, 1293, 1589
Bsc4I	CCNNNNNNNGG	2 cut(s)	149, 626
Bse1I	ACTGG	1 cut(s)	1114
Bse3DI	GCAATG	2 cut(s)	1105, 1301
Bse8I	GATNNNNATC	3 cut(s)	783, 789, 1578
BseBI	CCWGG	2 cut(s)	560, 621
BseDI	CCNNGG	5 cut(s)	558, 705, 805, 1293, 1589
BseGI	GGATG	8 cut(s)	80, 111, 235, 578, 976, 1399, 1538, 1579
BseJI	GATNNNNATC	3 cut(s)	783, 789, 1578
BseLI	CCNNNNNNNGG	2 cut(s)	149, 626
BseMI	GCAATG	2 cut(s)	1105, 1301
BseMII	CTCAG	5 cut(s)	80, 471, 825, 1239, 1695
BseNI	ACTGG	1 cut(s)	1114
BseRI	GAGGAG	1 cut(s)	108
BseSI	GKGCMC	1 cut(s)	1615
BseXI	GCAGC	4 cut(s)	112, 530, 1420, 1614
BshFI	GGCC	1 cut(s)	1251
BshNI	GGYRCC	2 cut(s)	740, 1010
BsiHKAI	GWGCWC	1 cut(s)	1224
BsiHKCI	CYCGRG	1 cut(s)	1555
BsiSI	CCGG	3 cut(s)	15, 707, 1295
BslFI	GGGAC	2 cut(s)	366, 1382
BslI	CCNNNNNNNGG	2 cut(s)	149, 626
BsmAI	GTCTC	2 cut(s)	1078, 1553
BsmFI	GGGAC	2 cut(s)	366, 1382
BsmI	GAATGC	3 cut(s)	115, 289, 1435
BsnI	GGCC	1 cut(s)	1251
Bso31I	GGTCTC	1 cut(s)	1553
BsoBI	CYCGRG	1 cut(s)	1555
Bsp119I	TTCGAA	1 cut(s)	436
Bsp1286I	GDGCHC	4 cut(s)	819, 1085, 1224, 1615
Bsp1407I	TGTACA	1 cut(s)	1330
Bsp143I	GATC	5 cut(s)	778, 784, 790, 1133, 1523
Bsp19I	CCATGG	2 cut(s)	805, 1589
BspACI	CCGC	8 cut(s)	103, 422, 495, 582, 688, 738, 887, 1542
BspANI	GGCC	1 cut(s)	1251
BspCNI	CTCAG	5 cut(s)	81, 472, 824, 1238, 1696
BspHI	TCATGA	1 cut(s)	781
BspLI	GGNNCC	5 cut(s)	382, 657, 742, 1012, 1525
BspPI	GGATC	3 cut(s)	798, 1518, 1531
BspT104I	TTCGAA	1 cut(s)	436
BspT107I	GGYRCC	2 cut(s)	740, 1010
BspTNI	GGTCTC	1 cut(s)	1553
BsrDI	GCAATG	2 cut(s)	1105, 1301
BsrGI	TGTACA	1 cut(s)	1330
BsrI	ACTGG	1 cut(s)	1114
BssECI	CCNNGG	5 cut(s)	558, 705, 805, 1293, 1589
BssMI	GATC	5 cut(s)	778, 784, 790, 1133, 1523
BssNAI	GTATAC	1 cut(s)	755
BssT1I	CCWWGG	2 cut(s)	805, 1589
Bst1107I	GTATAC	1 cut(s)	755
Bst2UI	CCWGG	2 cut(s)	560, 621
Bst4CI	ACNGT	3 cut(s)	64, 195, 922
Bst6I	CTCTTC	2 cut(s)	706, 1279
BstAPI	GCANNNNNTGC	2 cut(s)	636, 740
BstAUI	TGTACA	1 cut(s)	1330
BstBI	TTCGAA	1 cut(s)	436
BstC8I	GCNNGC	4 cut(s)	641, 736, 1648, 1731
BstDEI	CTNAG	7 cut(s)	89, 288, 480, 811, 1225, 1704, 1864
BstDSI	CCRYGG	2 cut(s)	805, 1589
BstF5I	GGATG	8 cut(s)	80, 111, 235, 578, 976, 1399, 1538, 1579
BstH2I	RGCGCY	1 cut(s)	1264
BstHHI	GCGC	2 cut(s)	1263, 1729
BstKTI	GATC	5 cut(s)	781, 787, 793, 1136, 1526
BstMAI	GTCTC	2 cut(s)	1078, 1553
BstMBI	GATC	5 cut(s)	778, 784, 790, 1133, 1523
BstMWI	GCNNNNNNNGC	5 cut(s)	636, 740, 1089, 1694, 1795
BstNI	CCWGG	2 cut(s)	560, 621
BstNSI	RCATGY	2 cut(s)	738, 1000
BstSCI	CCNGG	5 cut(s)	13, 558, 619, 705, 1293
BstSFI	CTRYAG	1 cut(s)	1689
BstSLI	GKGCMC	1 cut(s)	1615
BstV1I	GCAGC	4 cut(s)	112, 530, 1420, 1614
BstV2I	GAAGAC	1 cut(s)	1743
BstX2I	RGATCY	1 cut(s)	1523
BstXI	CCANNNNNNTGG	1 cut(s)	377
BstYI	RGATCY	1 cut(s)	1523
BstZ17I	GTATAC	1 cut(s)	755
BsuRI	GGCC	1 cut(s)	1251
BtgI	CCRYGG	2 cut(s)	805, 1589
BtsCI	GGATG	8 cut(s)	80, 111, 235, 578, 976, 1399, 1538, 1579
BtsI	GCAGTG	1 cut(s)	325
BtsIMutI	CAGTG	1 cut(s)	325
Cac8I	GCNNGC	4 cut(s)	641, 736, 1648, 1731
CaiI	CAGNNNCTG	1 cut(s)	1703
CciI	TCATGA	1 cut(s)	781
CfoI	GCGC	2 cut(s)	1263, 1729
Cfr13I	GGNCC	1 cut(s)	380
CsiI	ACCWGGT	1 cut(s)	619
Csp6I	GTAC	3 cut(s)	1011, 1035, 1331
CviAII	CATG	9 cut(s)	735, 782, 806, 966, 997, 1151, 1334, 1528, 1590
CviQI	GTAC	3 cut(s)	1011, 1035, 1331
DdeI	CTNAG	7 cut(s)	89, 288, 480, 811, 1225, 1704, 1864
DpnI	GATC	5 cut(s)	780, 786, 792, 1135, 1525
DpnII	GATC	5 cut(s)	778, 784, 790, 1133, 1523
EaeI	YGGCCR	1 cut(s)	1249
Eam1104I	CTCTTC	2 cut(s)	706, 1279
EarI	CTCTTC	2 cut(s)	706, 1279
Ecl136II	GAGCTC	1 cut(s)	1222
Eco130I	CCWWGG	2 cut(s)	805, 1589
Eco24I	GRGCYC	3 cut(s)	819, 1085, 1224
Eco31I	GGTCTC	1 cut(s)	1553
Eco32I	GATATC	1 cut(s)	1021
Eco47I	GGWCC	1 cut(s)	380
Eco53kI	GAGCTC	1 cut(s)	1222
Eco57I	CTGAAG	4 cut(s)	59, 432, 1647, 1755
Eco88I	CYCGRG	1 cut(s)	1555
EcoICRI	GAGCTC	1 cut(s)	1222
EcoRI	GAATTC	3 cut(s)	55, 826, 941
EcoRII	CCWGG	2 cut(s)	558, 619
EcoRV	GATATC	1 cut(s)	1021
EcoT14I	CCWWGG	2 cut(s)	805, 1589
EcoT22I	ATGCAT	1 cut(s)	1579
EcoT38I	GRGCYC	3 cut(s)	819, 1085, 1224
ErhI	CCWWGG	2 cut(s)	805, 1589
FaeI	CATG	9 cut(s)	738, 785, 809, 969, 1000, 1154, 1337, 1531, 1593
FaqI	GGGAC	2 cut(s)	366, 1382
FatI	CATG	9 cut(s)	734, 781, 805, 965, 996, 1150, 1333, 1527, 1589
FauI	CCCGC	1 cut(s)	681
FbaI	TGATCA	1 cut(s)	784
FblI	GTMKAC	1 cut(s)	754
Fnu4HI	GCNGC	5 cut(s)	101, 423, 519, 1434, 1603
FokI	GGATG	8 cut(s)	67, 118, 242, 565, 983, 1406, 1525, 1586
FriOI	GRGCYC	3 cut(s)	819, 1085, 1224
Fsp4HI	GCNGC	5 cut(s)	101, 423, 519, 1434, 1603
FspBI	CTAG	6 cut(s)	207, 255, 315, 750, 1307, 1876
GlaI	GCGC	2 cut(s)	1262, 1728
GluI	GCNGC	5 cut(s)	101, 423, 519, 1434, 1603
GsuI	CTGGAG	3 cut(s)	120, 1188, 1431
HaeII	RGCGCY	1 cut(s)	1264
HaeIII	GGCC	1 cut(s)	1251
HapII	CCGG	3 cut(s)	15, 707, 1295
HhaI	GCGC	2 cut(s)	1263, 1729
Hin1II	CATG	9 cut(s)	738, 785, 809, 969, 1000, 1154, 1337, 1531, 1593
Hin6I	GCGC	2 cut(s)	1261, 1727
HinP1I	GCGC	2 cut(s)	1261, 1727
HincII	GTYRAC	2 cut(s)	1119, 1374
HindII	GTYRAC	2 cut(s)	1119, 1374
HindIII	AAGCTT	1 cut(s)	1636
HpaII	CCGG	3 cut(s)	15, 707, 1295
HphI	GGTGA	4 cut(s)	509, 609, 721, 1404
Hpy166II	GTNNAC	7 cut(s)	238, 755, 868, 1035, 1059, 1119, 1374
Hpy8I	GTNNAC	7 cut(s)	238, 755, 868, 1035, 1059, 1119, 1374
HpyAV	CCTTC	4 cut(s)	410, 900, 1622, 1625
HpyCH4III	ACNGT	3 cut(s)	64, 195, 922
HpyCH4V	TGCA	8 cut(s)	245, 518, 1461, 1531, 1577, 1602, 1744, 1798
HpyF10VI	GCNNNNNNNGC	5 cut(s)	636, 740, 1089, 1694, 1795
HpyF3I	CTNAG	7 cut(s)	89, 288, 480, 811, 1225, 1704, 1864
Hsp92II	CATG	9 cut(s)	738, 785, 809, 969, 1000, 1154, 1337, 1531, 1593
HspAI	GCGC	2 cut(s)	1261, 1727
KpnI	GGTACC	1 cut(s)	1014
Ksp22I	TGATCA	1 cut(s)	784
Kzo9I	GATC	5 cut(s)	778, 784, 790, 1133, 1523
LmnI	GCTCC	2 cut(s)	13, 1498
Lsp1109I	GCAGC	4 cut(s)	112, 530, 1420, 1614
LweI	GCATC	4 cut(s)	544, 1101, 1564, 1714
MabI	ACCWGGT	1 cut(s)	619
MaeI	CTAG	6 cut(s)	207, 255, 315, 750, 1307, 1876
MaeIII	GTNAC	4 cut(s)	475, 497, 1198, 1783
MalI	GATC	5 cut(s)	780, 786, 792, 1135, 1525
MboI	GATC	5 cut(s)	778, 784, 790, 1133, 1523
MboII	GAAGA	8 cut(s)	52, 337, 425, 693, 1266, 1434, 1574, 1748
MfeI	CAATTG	1 cut(s)	570
MflI	RGATCY	1 cut(s)	1523
MhlI	GDGCHC	4 cut(s)	819, 1085, 1224, 1615
MlsI	TGGCCA	1 cut(s)	1251
MluNI	TGGCCA	1 cut(s)	1251
MlyI	GAGTC	2 cut(s)	968, 994
Mox20I	TGGCCA	1 cut(s)	1251
Mph1103I	ATGCAT	1 cut(s)	1579
MroXI	GAANNNNTTC	1 cut(s)	1698
MscI	TGGCCA	1 cut(s)	1251
MseI	TTAA	6 cut(s)	542, 1142, 1355, 1382, 1455, 1569
MslI	CAYNNNNRTG	1 cut(s)	224
Msp20I	TGGCCA	1 cut(s)	1251
MspA1I	CMGCKG	4 cut(s)	103, 521, 1544, 1733
MspI	CCGG	3 cut(s)	15, 707, 1295
MspR9I	CCNGG	5 cut(s)	15, 560, 621, 707, 1295
MunI	CAATTG	1 cut(s)	570
Mva1269I	GAATGC	3 cut(s)	115, 289, 1435
MvaI	CCWGG	2 cut(s)	560, 621
MwoI	GCNNNNNNNGC	5 cut(s)	636, 740, 1089, 1694, 1795
NciI	CCSGG	3 cut(s)	15, 707, 1295
NcoI	CCATGG	2 cut(s)	805, 1589
NdeII	GATC	5 cut(s)	778, 784, 790, 1133, 1523
NlaIII	CATG	9 cut(s)	738, 785, 809, 969, 1000, 1154, 1337, 1531, 1593
NlaIV	GGNNCC	5 cut(s)	382, 657, 742, 1012, 1525
NmuCI	GTSAC	2 cut(s)	497, 1198
NsiI	ATGCAT	1 cut(s)	1579
NspI	RCATGY	2 cut(s)	738, 1000
NspV	TTCGAA	1 cut(s)	436
PaeI	GCATGC	1 cut(s)	738
PaeR7I	CTCGAG	1 cut(s)	1555
PagI	TCATGA	1 cut(s)	781
PctI	GAATGC	3 cut(s)	115, 289, 1435
PdmI	GAANNNNTTC	1 cut(s)	1698
PfeI	GAWTC	8 cut(s)	131, 202, 328, 395, 563, 718, 760, 1298
PkrI	GCNGC	5 cut(s)	102, 424, 520, 1435, 1604
PleI	GAGTC	2 cut(s)	968, 993
PpsI	GAGTC	2 cut(s)	968, 993
Psp124BI	GAGCTC	1 cut(s)	1224
Psp6I	CCWGG	2 cut(s)	558, 619
PspGI	CCWGG	2 cut(s)	558, 619
PspN4I	GGNNCC	5 cut(s)	382, 657, 742, 1012, 1525
PspPI	GGNCC	1 cut(s)	380
PspXI	VCTCGAGB	1 cut(s)	1555
PsrI	GAACNNNNNNTAC	2 cut(s)	1683, 1715
PstNI	CAGNNNCTG	1 cut(s)	1703
PsuI	RGATCY	1 cut(s)	1523
PvuII	CAGCTG	2 cut(s)	521, 1733
RsaI	GTAC	3 cut(s)	1012, 1036, 1332
RsaNI	GTAC	3 cut(s)	1011, 1035, 1331
RseI	CAYNNNNRTG	1 cut(s)	224
SacI	GAGCTC	1 cut(s)	1224
SaqAI	TTAA	6 cut(s)	542, 1142, 1355, 1382, 1455, 1569
SatI	GCNGC	5 cut(s)	101, 423, 519, 1434, 1603
Sau3AI	GATC	5 cut(s)	778, 784, 790, 1133, 1523
Sau96I	GGNCC	1 cut(s)	380
SchI	GAGTC	2 cut(s)	968, 994
ScrFI	CCNGG	5 cut(s)	15, 560, 621, 707, 1295
SduI	GDGCHC	4 cut(s)	819, 1085, 1224, 1615
SexAI	ACCWGGT	1 cut(s)	619
SfaNI	GCATC	4 cut(s)	544, 1101, 1564, 1714
SfcI	CTRYAG	1 cut(s)	1689
Sfr274I	CTCGAG	1 cut(s)	1555
SfuI	TTCGAA	1 cut(s)	436
SinI	GGWCC	1 cut(s)	380
SlaI	CTCGAG	1 cut(s)	1555
SmiMI	CAYNNNNRTG	1 cut(s)	224
SmlI	CTYRAG	4 cut(s)	764, 946, 1264, 1555
SmoI	CTYRAG	4 cut(s)	764, 946, 1264, 1555
SpeI	ACTAGT	1 cut(s)	749
SphI	GCATGC	1 cut(s)	738
SsiI	CCGC	8 cut(s)	103, 422, 495, 582, 688, 738, 887, 1542
SspMI	CTAG	6 cut(s)	207, 255, 315, 750, 1307, 1876
SstI	GAGCTC	1 cut(s)	1224
StyD4I	CCNGG	5 cut(s)	13, 558, 619, 705, 1293
StyI	CCWWGG	2 cut(s)	805, 1589
TaaI	ACNGT	3 cut(s)	64, 195, 922
TaqI	TCGA	4 cut(s)	436, 525, 824, 1556
TatI	WGTACW	2 cut(s)	1034, 1330
TauI	GCSGC	1 cut(s)	425
TfiI	GAWTC	8 cut(s)	131, 202, 328, 395, 563, 718, 760, 1298
Tru1I	TTAA	6 cut(s)	542, 1142, 1355, 1382, 1455, 1569
Tru9I	TTAA	6 cut(s)	542, 1142, 1355, 1382, 1455, 1569
TscAI	CASTG	1 cut(s)	325
TseFI	GTSAC	2 cut(s)	497, 1198
TseI	GCWGC	4 cut(s)	100, 518, 1433, 1602
Tsp45I	GTSAC	2 cut(s)	497, 1198
TspDTI	ATGAA	6 cut(s)	122, 144, 246, 477, 710, 1670
TspGWI	ACGGA	2 cut(s)	458, 1478
TspRI	CASTG	1 cut(s)	325
VpaK11BI	GGWCC	1 cut(s)	380
XapI	RAATTY	4 cut(s)	55, 826, 941, 1243
XceI	RCATGY	2 cut(s)	738, 1000
XhoI	CTCGAG	1 cut(s)	1555
XmiI	GTMKAC	1 cut(s)	754
XmnI	GAANNNNTTC	1 cut(s)	1698
XspI	CTAG	6 cut(s)	207, 255, 315, 750, 1307, 1876
Zsp2I	ATGCAT	1 cut(s)	1579

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.