MD12G1072400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015659
Physical Location & Seq
Reverse (-)
8293658 .. 8298235
4578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1072400.v1.1.491

Sequence Viewer

Length: 2424 bp
ATGGAGATGGCAACCTGCACCTTCCACTGCTCCTCCTCCTCCATCCCGAACCCTAGAAGCACTAGCATTTCCTCTCCTCTTCCAGTCGCCAAGCAACTCTACGCCTTCACCTTACCTTCTTCCTCAACCGCCAAAATAACCACAATCTCCGCTCCCCGAAACCCTTCTCCACTCACCCGCCAGTCAAATGCTTCGTCTCCGAAGCCACTGAAGCCACCGCCACCGAAGAAGATCGCCACTGTGGAGGTGCCAGAAGAACATATGGACCCTGTTGTTGAGCTTACTGGCAAAAGGCTGGAAGGCACAACCTTCCTCAAACAACGAGAATACACACTTCTCTTCAATGACTGGCCTCAGCTCCTCCAACTCTCAATTGGGTCCCAAATCCTCATTCTGGGTCAGGCAATTCATGCATTTTTACTCAAATGTGGCTGCCAGAATGACACATTTCAGGGCAACAATCTTGTCAACTTGTACTCGAAATTCAAAAGACTGGATGATGCACATCACCTTTTCGATGAAATGCCTGTTAGAAACACAATCACTTGGACTTCTCTCATGAAGGGGTATTCTGATATTGGTGACCTTGAATCTGTCTTCCACGTTGCACGTGATATGTTTTGCAGCGACGAGAAGCTTAACGAGCATACATGCTCAGTGATTTTGGAGGCGTGTAGTTCACTTGAAGACCGGAGAAGTGGGGAACAGGTTCACGGTTTTGTTGTAAAAAGTGGGCTTCAGGAGAATGTTTTTGTGGCCACTTCTTTGGTTTCTTTGTACTCTAGAAGTGGCTTCTTGGGTGGTGCAGAGAAAGTGTTCAATGACATGGATTACAAAGATGTTCAGTGTTTAAATTATATGATTTTAGAATATGGGAAGGCGGGCTGTGGAGAGAAAGCAATTGGGGTCTTTATAGATCTTCTGAGTTCTGGCTTAGAGCCAAATGATTATACTTTTACCAATCTGATTAGTGCATGCGTTGGAGATGTGGGTGTGGATGAATGCTTGCAATTACATGGGCTTGCTGTCAAGTATGGTATTATGGGTGAAACTTCTGTTGGAAATGCAATAATAACCATGTATGGAAAGCACGGGATGGTTGAAGAAGCTGAGGCAATGTTTTATTTACTGGATGAGAGAAACTTAATTTCTTGGACCGCACTTTTGTCAATGTATGTTAAAAATGGCCGTGGGGATATGGCCCTTGATGCTGTCTTAAAAATACTTGATCAAGGTATTTGTGTTGATTCTAGCTGTTTATCTACCGTGCTTGATGCCTGTTCAGAGTGCAGAAATCTGGAATTGGGATGTCAGATCCATGCATTTTGCATAAAGCTTGATTTTGACTCTTGCGTCAATGTTGGGACTGCGTTGATTGACACATACGCTAAATGTGGGAGCCTTCAATCTGCAAGACAGGTTTTTAATGGCCTTTCAGGTAAAAACACCGCTTCATTTAATGCACTACTTGTTGGATTCATGGAACAACATAGAGATGTTGAAGAGGATTCCATGGTCTTATTTAGTCAGTTAAGGTCGTCTGGTGTAAATCCAGATTTCATAACGTATTCACGACTCCTTAGTGTAGCAGCTGAAGAAGCTTGCTTAGAAAGGGGGAAGAGTCTCCATGCTTGCACTATTAAAGCTGGATTTGAAGCCAATTCCACTGTATCTAATGCTGTAATTACCATGTATGCCAAATGTGGTAGCATTGAAGAAGCTTATCAAATGTTCAATGGTATGAATAGTCGTGATTGCGTATCCTGGAATGCCATAATTTCTGCACATGCCCTTCATGGAGAAGGCAACAAAGCACTTTCACTTTTTGAATCAATGAAGGGAGAAGGGTTTGCCCCTGATGAGCTCACGATGTTGGCTACTCTTCAAGCTTGCAGTTACAATGGACTATGGGAAACTGGGTTACGCTTATTCAATGAAATGGAGCCAAAATATGGAACTAAGTCAGGAATTGAGCACTTTGCATGCATGGTGGATCTTCTGGGTAGGTCTGGGAATTTTTCAGAAGCCATCGATTTCATCCACAGAAGTCCATTTCCAGATTCACCTATGCTTTGGAGAACTTTAGTGAATGTGTGCATGTTAAGTGGGAATTTGAATTATGGCATGTTAGCCTCAAAACGTTTGCTTGACTTGCAACCTGAGGACGCTGGGTCTTACATACTTGTTTCAAATATGTATGCTCGAGGAGGAATGTTTGATGAGGCTTCAAAGGTTCGAACCGTTATGAATGACTTGAAAGTGAACAAAGAAGCAGGATGCAGCTGGATTGAAGTTGATAATAAATTTCATTATTTTGTTGCAAGTGATATGGACCATCCAAAAAGTGCTGAAATTTATGCTGAACTCAATCTATTAAAGGTTTTAATAAACAAAACAGTGAGGATAGATGTGATCTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

808

Amino Acids

88.83

Weight (kDa)

5.23

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 178 - 226 1.6e-06 PPR repeat family
PPR_2 PF13041 280 - 327 3.2e-08 PPR repeat family
PPR_2 PF13041 585 - 631 1.5e-08 PPR repeat family
PPR PF01535 587 - 617 1.4e-06 PPR repeat
E_motif PF20431 704 - 764 2.8e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g23520
malus_domestica MD12G1072400.v1.1 MD14G1069600.v1.1
pyrus_communis pycom12g06080 pycom14g05770
rosa_chinensis RchiOBHm_Chr3g0481521
rosa_laevigata RLG00000023390
rosa_multiflora Rmu_co8224982.1_g000001 Rmu_sc0010846.1_g000006
rosa_roxburghii Rroxscaffold_6G00400370
rosa_rugosa Rorug03G0192400
rosa_samantha Rh3AG243600 Rh3BG278500 Rh3CG273700 Rh3DG270500
rosa_wichuraiana Rw3G021760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1352
Acc36I ACCTGC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 247
AccB7I CCANNNNNTGG 1 cut(s) 1952
AccBSI CCGCTC 1 cut(s) 152
AciI CCGC 7 cut(s) 129, 150, 178, 218, 881, 1158, 1449
AclI AACGTT 1 cut(s) 2140
AclWI GGATC 2 cut(s) 1309, 2001
AcoI YGGCCR 2 cut(s) 756, 1186
AcsI RAATTY 5 cut(s) 482, 2014, 2110, 2303, 2352
AcuI CTGAAG 3 cut(s) 230, 722, 1614
AcvI CACGTG 1 cut(s) 611
AfaI GTAC 2 cut(s) 476, 779
AfiI CCNNNNNNNGG 2 cut(s) 394, 1952
AhdI GACNNNNNGTC 1 cut(s) 2170
AjnI CCWGG 1 cut(s) 1763
AjuI GAANNNNNNNTTGG 4 cut(s) 375, 407, 1041, 1073
Alw21I GWGCWC 2 cut(s) 1866, 1977
Alw26I GTCTC 2 cut(s) 201, 1628
AlwI GGATC 2 cut(s) 1309, 2001
Ama87I CYCGRG 1 cut(s) 2202
AoxI GGCC 5 cut(s) 350, 756, 1186, 1200, 1429
ApeKI GCWGC 4 cut(s) 432, 624, 1589, 2280
ApoI RAATTY 5 cut(s) 482, 2014, 2110, 2303, 2352
ArsI GACNNNNNNTTYG 4 cut(s) 179, 211, 1147, 1179
Asp700I GAANNNNTTC 3 cut(s) 163, 708, 815
AspS9I GGNCC 5 cut(s) 265, 378, 1155, 1201, 2332
AsuHPI GGTGA 6 cut(s) 100, 166, 500, 593, 1058, 2055
AsuII TTCGAA 1 cut(s) 2236
AvaI CYCGRG 1 cut(s) 2202
AvaII GGWCC 4 cut(s) 265, 378, 1155, 2332
AxyI CCTNAGG 1 cut(s) 2160
BalI TGGCCA 1 cut(s) 758
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 1866
BbrPI CACGTG 1 cut(s) 611
BbsI GAAGAC 2 cut(s) 589, 693
Bbv12I GWGCWC 2 cut(s) 1866, 1977
BbvCI CCTCAGC 2 cut(s) 354, 1110
BbvI GCAGC 4 cut(s) 419, 636, 1601, 2292
BccI CCATC 4 cut(s) 50, 1090, 2036, 2343
BceAI ACGGC 1 cut(s) 1173
BciT130I CCWGG 1 cut(s) 1765
BciVI GTATCC 1 cut(s) 1771
BclI TGATCA 1 cut(s) 1228
BcoDI GTCTC 2 cut(s) 201, 1628
BfaI CTAG 4 cut(s) 54, 63, 783, 1251
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 1 cut(s) 1771
BglII AGATCT 1 cut(s) 916
BisI GCNGC 4 cut(s) 433, 625, 1590, 2281
BlsI GCNGC 4 cut(s) 434, 626, 1591, 2282
Bme1390I CCNGG 1 cut(s) 1765
Bme18I GGWCC 4 cut(s) 265, 378, 1155, 2332
BmeRI GACNNNNNGTC 1 cut(s) 2170
BmeT110I CYCGRG 1 cut(s) 2202
BmgT120I GGNCC 5 cut(s) 265, 378, 1155, 1201, 2332
BmiI GGNNCC 6 cut(s) 249, 267, 379, 380, 1400, 1944
BmrFI CCNGG 1 cut(s) 1765
BmrI ACTGGG 1 cut(s) 1926
BmsI GCATC 4 cut(s) 490, 1198, 1264, 2267
BmuI ACTGGG 1 cut(s) 1926
BpiI GAAGAC 2 cut(s) 589, 693
Bpu10I CCTNAGC 2 cut(s) 354, 1110
Bpu14I TTCGAA 1 cut(s) 2236
Bsa29I ATCGAT 1 cut(s) 2031
BsaAI YACGTR 1 cut(s) 611
BsaBI GATNNNNATC 2 cut(s) 504, 2417
BsaJI CCNNGG 2 cut(s) 1189, 1512
BsaWI WCCGGW 1 cut(s) 690
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 1952
Bse1I ACTGG 7 cut(s) 83, 181, 289, 353, 498, 1134, 1921
Bse21I CCTNAGG 1 cut(s) 2160
Bse3DI GCAATG 1 cut(s) 1122
Bse8I GATNNNNATC 2 cut(s) 504, 2417
BseBI CCWGG 1 cut(s) 1765
BseCI ATCGAT 1 cut(s) 2031
BseDI CCNNGG 2 cut(s) 1189, 1512
BseGI GGATG 9 cut(s) 42, 502, 1003, 1101, 1138, 1313, 2037, 2282, 2335
BseJI GATNNNNATC 2 cut(s) 504, 2417
BseLI CCNNNNNNNGG 2 cut(s) 394, 1952
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 5 cut(s) 368, 669, 914, 1101, 2151
BseNI ACTGG 7 cut(s) 83, 181, 289, 353, 498, 1134, 1921
BseRI GAGGAG 6 cut(s) 22, 25, 28, 66, 350, 2220
BseXI GCAGC 4 cut(s) 419, 636, 1601, 2292
BseYI CCCAGC 1 cut(s) 2168
BsgI GTGCAG 3 cut(s) 825, 1309, 1767
BshFI GGCC 5 cut(s) 352, 758, 1188, 1202, 1431
BshNI GGYRCC 1 cut(s) 247
BshVI ATCGAT 1 cut(s) 2031
BsiHKAI GWGCWC 2 cut(s) 1866, 1977
BsiHKCI CYCGRG 1 cut(s) 2202
BsiSI CCGG 1 cut(s) 691
BslFI GGGAC 2 cut(s) 364, 1378
BslI CCNNNNNNNGG 2 cut(s) 394, 1952
BsmAI GTCTC 2 cut(s) 201, 1628
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 2 cut(s) 364, 1378
BsmI GAATGC 2 cut(s) 1007, 1774
BsnI GGCC 5 cut(s) 352, 758, 1188, 1202, 1431
BsoBI CYCGRG 1 cut(s) 2202
Bsp119I TTCGAA 1 cut(s) 2236
Bsp1286I GDGCHC 2 cut(s) 1866, 1977
Bsp143I GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
Bsp19I CCATGG 1 cut(s) 1512
BspACI CCGC 7 cut(s) 129, 150, 178, 218, 881, 1158, 1449
BspANI GGCC 5 cut(s) 352, 758, 1188, 1202, 1431
BspCNI CTCAG 5 cut(s) 367, 668, 915, 1102, 2152
BspDI ATCGAT 1 cut(s) 2031
BspHI TCATGA 1 cut(s) 558
BspLI GGNNCC 6 cut(s) 249, 267, 379, 380, 1400, 1944
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 2 cut(s) 1309, 2001
BspT104I TTCGAA 1 cut(s) 2236
BspT107I GGYRCC 1 cut(s) 247
BsrBI CCGCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 1122
BsrI ACTGG 7 cut(s) 83, 181, 289, 353, 498, 1134, 1921
BssECI CCNNGG 2 cut(s) 1189, 1512
BssMI GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
BssT1I CCWWGG 1 cut(s) 1512
Bst2UI CCWGG 1 cut(s) 1765
Bst4CI ACNGT 6 cut(s) 241, 716, 1267, 1669, 2242, 2398
Bst6I CTCTTC 5 cut(s) 84, 344, 1497, 1613, 1887
BstAPI GCANNNNNTGC 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 611
BstBI TTCGAA 1 cut(s) 2236
BstC8I GCNNGC 8 cut(s) 883, 976, 1007, 1023, 1603, 1633, 1891, 1984
BstDEI CTNAG 9 cut(s) 354, 655, 923, 934, 1110, 1580, 1606, 1959, 2160
BstDSI CCRYGG 2 cut(s) 1189, 1512
BstEII GGTNACC 1 cut(s) 581
BstF5I GGATG 9 cut(s) 42, 502, 1003, 1101, 1138, 1313, 2037, 2282, 2335
BstKTI GATC 6 cut(s) 234, 919, 1231, 1317, 1996, 2415
BstMAI GTCTC 2 cut(s) 201, 1628
BstMBI GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
BstMWI GCNNNNNNNGC 6 cut(s) 211, 410, 643, 1208, 1598, 2152
BstNI CCWGG 1 cut(s) 1765
BstNSI RCATGY 6 cut(s) 654, 978, 1790, 1986, 2101, 2128
BstPI GGTNACC 1 cut(s) 581
BstSCI CCNGG 1 cut(s) 1763
BstV1I GCAGC 4 cut(s) 419, 636, 1601, 2292
BstV2I GAAGAC 2 cut(s) 589, 693
BstX2I RGATCY 3 cut(s) 916, 1314, 1993
BstXI CCANNNNNNTGG 1 cut(s) 766
BstYI RGATCY 3 cut(s) 916, 1314, 1993
Bsu15I ATCGAT 1 cut(s) 2031
Bsu36I CCTNAGG 1 cut(s) 2160
BsuI GTATCC 1 cut(s) 1771
BsuRI GGCC 5 cut(s) 352, 758, 1188, 1202, 1431
BsuTUI ATCGAT 1 cut(s) 2031
BtgI CCRYGG 2 cut(s) 1189, 1512
BtsCI GGATG 9 cut(s) 42, 502, 1003, 1101, 1138, 1313, 2037, 2282, 2335
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 7 cut(s) 25, 206, 237, 663, 851, 1665, 2403
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 8 cut(s) 883, 976, 1007, 1023, 1603, 1633, 1891, 1984
CciI TCATGA 1 cut(s) 558
Cfr13I GGNCC 5 cut(s) 265, 378, 1155, 1201, 2332
ClaI ATCGAT 1 cut(s) 2031
CseI GACGC 2 cut(s) 1342, 2174
Csp6I GTAC 2 cut(s) 475, 778
CviQI GTAC 2 cut(s) 475, 778
DdeI CTNAG 9 cut(s) 354, 655, 923, 934, 1110, 1580, 1606, 1959, 2160
DpnI GATC 6 cut(s) 233, 918, 1230, 1316, 1995, 2414
DpnII GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
DraI TTTAAA 1 cut(s) 852
DrdI GACNNNNNNGTC 1 cut(s) 1352
DriI GACNNNNNGTC 1 cut(s) 2170
DseDI GACNNNNNNGTC 1 cut(s) 1352
EaeI YGGCCR 2 cut(s) 756, 1186
Eam1104I CTCTTC 5 cut(s) 84, 344, 1497, 1613, 1887
Eam1105I GACNNNNNGTC 1 cut(s) 2170
EarI CTCTTC 5 cut(s) 84, 344, 1497, 1613, 1887
Ecl136II GAGCTC 1 cut(s) 1864
Eco130I CCWWGG 1 cut(s) 1512
Eco24I GRGCYC 1 cut(s) 1866
Eco47I GGWCC 4 cut(s) 265, 378, 1155, 2332
Eco53kI GAGCTC 1 cut(s) 1864
Eco57I CTGAAG 3 cut(s) 230, 722, 1614
Eco72I CACGTG 1 cut(s) 611
Eco81I CCTNAGG 1 cut(s) 2160
Eco88I CYCGRG 1 cut(s) 2202
Eco91I GGTNACC 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 1864
EcoO109I RGGNCCY 1 cut(s) 378
EcoO65I GGTNACC 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 1763
EcoT14I CCWWGG 1 cut(s) 1512
EcoT22I ATGCAT 3 cut(s) 415, 1324, 1988
EcoT38I GRGCYC 1 cut(s) 1866
ErhI CCWWGG 1 cut(s) 1512
Esp3I CGTCTC 1 cut(s) 201
FaqI GGGAC 2 cut(s) 364, 1378
FauI CCCGC 2 cut(s) 185, 874
FauNDI CATATG 1 cut(s) 261
FbaI TGATCA 1 cut(s) 1228
Fnu4HI GCNGC 4 cut(s) 433, 625, 1590, 2281
FokI GGATG 9 cut(s) 29, 509, 1010, 1108, 1145, 1320, 2024, 2289, 2322
FriOI GRGCYC 1 cut(s) 1866
Fsp4HI GCNGC 4 cut(s) 433, 625, 1590, 2281
FspBI CTAG 4 cut(s) 54, 63, 783, 1251
GluI GCNGC 4 cut(s) 433, 625, 1590, 2281
GsaI CCCAGC 1 cut(s) 2172
HaeIII GGCC 5 cut(s) 352, 758, 1188, 1202, 1431
HapII CCGG 1 cut(s) 691
HgaI GACGC 2 cut(s) 1342, 2174
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 5 cut(s) 635, 1334, 1599, 1719, 1887
HinfI GANTC 9 cut(s) 590, 1247, 1346, 1476, 1508, 1575, 1621, 1829, 2060
HpaII CCGG 1 cut(s) 691
HphI GGTGA 6 cut(s) 100, 166, 500, 593, 1058, 2055
Hpy166II GTNNAC 4 cut(s) 469, 680, 712, 2263
Hpy188I TCNGA 8 cut(s) 201, 574, 924, 966, 1285, 1314, 2023, 2423
Hpy8I GTNNAC 4 cut(s) 469, 680, 712, 2263
Hpy99I CGWCG 1 cut(s) 632
HpyCH4III ACNGT 6 cut(s) 241, 716, 1267, 1669, 2242, 2398
HpyCH4IV ACGT 4 cut(s) 603, 610, 1565, 2140
HpyF10VI GCNNNNNNNGC 6 cut(s) 211, 410, 643, 1208, 1598, 2152
HpyF3I CTNAG 9 cut(s) 354, 655, 923, 934, 1110, 1580, 1606, 1959, 2160
HpySE526I ACGT 4 cut(s) 603, 610, 1565, 2140
KflI GGGWCCC 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 1228
Kzo9I GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
LmnI GCTCC 5 cut(s) 35, 157, 363, 1398, 1942
Lsp1109I GCAGC 4 cut(s) 419, 636, 1601, 2292
LweI GCATC 4 cut(s) 490, 1198, 1264, 2267
MaeI CTAG 4 cut(s) 54, 63, 783, 1251
MaeII ACGT 4 cut(s) 603, 610, 1565, 2140
MaeIII GTNAC 3 cut(s) 581, 1895, 1920
MalI GATC 6 cut(s) 233, 918, 1230, 1316, 1995, 2414
MbiI CCGCTC 1 cut(s) 152
MboI GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
MfeI CAATTG 2 cut(s) 372, 900
MflI RGATCY 3 cut(s) 916, 1314, 1993
MhlI GDGCHC 2 cut(s) 1866, 1977
MlsI TGGCCA 1 cut(s) 758
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 3 cut(s) 1340, 1569, 1630
MmeI TCCRAC 4 cut(s) 388, 961, 1039, 1453
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 3 cut(s) 415, 1324, 1988
MroXI GAANNNNTTC 3 cut(s) 163, 708, 815
MscI TGGCCA 1 cut(s) 758
MslI CAYNNNNRTG 1 cut(s) 1494
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 2 cut(s) 1592, 2283
MspI CCGG 1 cut(s) 691
MspR9I CCNGG 1 cut(s) 1765
MunI CAATTG 2 cut(s) 372, 900
Mva1269I GAATGC 2 cut(s) 1007, 1774
MvaI CCWGG 1 cut(s) 1765
MwoI GCNNNNNNNGC 6 cut(s) 211, 410, 643, 1208, 1598, 2152
NcoI CCATGG 1 cut(s) 1512
NdeI CATATG 1 cut(s) 261
NdeII GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
NlaIV GGNNCC 6 cut(s) 249, 267, 379, 380, 1400, 1944
NmuCI GTSAC 1 cut(s) 581
NsiI ATGCAT 3 cut(s) 415, 1324, 1988
NspI RCATGY 6 cut(s) 654, 978, 1790, 1986, 2101, 2128
NspV TTCGAA 1 cut(s) 2236
PaeI GCATGC 2 cut(s) 978, 1986
PaeR7I CTCGAG 1 cut(s) 2202
PagI TCATGA 1 cut(s) 558
PctI GAATGC 2 cut(s) 1007, 1774
PdmI GAANNNNTTC 3 cut(s) 163, 708, 815
PfeI GAWTC 6 cut(s) 590, 1247, 1476, 1508, 1829, 2060
PflMI CCANNNNNTGG 1 cut(s) 1952
PfoI TCCNGGA 1 cut(s) 1763
PkrI GCNGC 4 cut(s) 434, 626, 1591, 2282
PleI GAGTC 3 cut(s) 1340, 1569, 1629
PmaCI CACGTG 1 cut(s) 611
PmlI CACGTG 1 cut(s) 611
PpsI GAGTC 3 cut(s) 1340, 1569, 1629
Ppu21I YACGTR 1 cut(s) 611
PpuMI RGGWCCY 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 1866
Psp1406I AACGTT 1 cut(s) 2140
Psp5II RGGWCCY 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 1763
PspCI CACGTG 1 cut(s) 611
PspEI GGTNACC 1 cut(s) 581
PspFI CCCAGC 1 cut(s) 2168
PspGI CCWGG 1 cut(s) 1763
PspN4I GGNNCC 6 cut(s) 249, 267, 379, 380, 1400, 1944
PspPI GGNCC 5 cut(s) 265, 378, 1155, 1201, 2332
PspPPI RGGWCCY 1 cut(s) 378
PspXI VCTCGAGB 1 cut(s) 2202
PsuI RGATCY 3 cut(s) 916, 1314, 1993
PvuII CAGCTG 2 cut(s) 1592, 2283
RsaI GTAC 2 cut(s) 476, 779
RsaNI GTAC 2 cut(s) 475, 778
RseI CAYNNNNRTG 1 cut(s) 1494
SacI GAGCTC 1 cut(s) 1866
SatI GCNGC 4 cut(s) 433, 625, 1590, 2281
Sau3AI GATC 6 cut(s) 231, 916, 1228, 1314, 1993, 2412
Sau96I GGNCC 5 cut(s) 265, 378, 1155, 1201, 2332
SchI GAGTC 3 cut(s) 1340, 1569, 1630
ScrFI CCNGG 1 cut(s) 1765
SduI GDGCHC 2 cut(s) 1866, 1977
SfaNI GCATC 4 cut(s) 490, 1198, 1264, 2267
Sfr274I CTCGAG 1 cut(s) 2202
SfuI TTCGAA 1 cut(s) 2236
SinI GGWCC 4 cut(s) 265, 378, 1155, 2332
SlaI CTCGAG 1 cut(s) 2202
SmiMI CAYNNNNRTG 1 cut(s) 1494
SmlI CTYRAG 1 cut(s) 2202
SmoI CTYRAG 1 cut(s) 2202
SphI GCATGC 2 cut(s) 978, 1986
SsiI CCGC 7 cut(s) 129, 150, 178, 218, 881, 1158, 1449
SspMI CTAG 4 cut(s) 54, 63, 783, 1251
SstI GAGCTC 1 cut(s) 1866
StyD4I CCNGG 1 cut(s) 1763
StyI CCWWGG 1 cut(s) 1512
TaaI ACNGT 6 cut(s) 241, 716, 1267, 1669, 2242, 2398
TaiI ACGT 4 cut(s) 606, 613, 1568, 2143
TaqI TCGA 5 cut(s) 479, 516, 2031, 2203, 2236
TatI WGTACW 2 cut(s) 474, 777
TfiI GAWTC 6 cut(s) 590, 1247, 1476, 1508, 1829, 2060
TscAI CASTG 7 cut(s) 32, 213, 244, 663, 851, 1672, 2403
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 4 cut(s) 432, 624, 1589, 2280
Tsp45I GTSAC 1 cut(s) 581
TspRI CASTG 7 cut(s) 32, 213, 244, 663, 851, 1672, 2403
Van91I CCANNNNNTGG 1 cut(s) 1952
VpaK11BI GGWCC 4 cut(s) 265, 378, 1155, 2332
XapI RAATTY 5 cut(s) 482, 2014, 2110, 2303, 2352
XbaI TCTAGA 1 cut(s) 782
XceI RCATGY 6 cut(s) 654, 978, 1790, 1986, 2101, 2128
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 2202
XmnI GAANNNNTTC 3 cut(s) 163, 708, 815
XspI CTAG 4 cut(s) 54, 63, 783, 1251
Zsp2I ATGCAT 3 cut(s) 415, 1324, 1988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.