Rw3G021760

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015659
Physical Location & Seq
Reverse (-)
26379104 .. 26381392
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G021760.1

Sequence Viewer

Length: 2172 bp
ATGGATACCCTTCTCTCTAACTCCTTGCTTCCCGCTCTTTACGTACCCCAACGACCCTTCCCTCCATTGAAGAACACCATCAGCAAGTCCTCTTCAATCCGAAACCCAAAGCCCAAGTCACTCCATTTTTCTACCAACTCAACTCTTCCGGCCTCTCCACAACAAGCACAACGCAGCCTTCTCTTCAATGAATGGCCTCAGCTCCTCAAATTCTCAATTGGGTCCAAAAATCTGATGCTGGGTCAGGCAATTCATGCCTTCTTACTCAAATGCCGGTGCCAAACTGACACCGACACATTTCAAGGCAACAATCTCGTCAACATGTACTCGAAATTCAAAAGATTGGATGATGCACACCGGGTATTCGATGAAATGCCTGAGAGAAACACAATCACTTGGACTGCTCTAATGAAGGGGTATTCTGAGGTTGGCGATTATGCATCTCTTTTTCGCATTGCTCGGGACATGTTTCGGGGGGAGGAGAAGTTTAATGAGCATACTTGCTCTGTGATTTTGGAGGCGTGTGGTTCACTAGAAGAGTCAAGACGTGGGGAACAGGTTCATGGTTTTGTTGTAAAAAGTGGGCTTCAGGACAACGTTTTTGTAGCTACTTCATTGGTCTCTATGTACTCTAGAAGTGACTGCTTGGGCAATGCCGAGAAAGTGTTCAGTGACATGGATTACAAAGATATCCAGTGCTTAAATTATATGATTTTAGAATATGGGAGGGCCGGCCTCGGAGAGAAAGCAATTGGGGTTTTTATTGATCTTCTGAGTTCTGGTATAGATCCAAATGATTATACATTTACTAATTTAATCAGTGCATGCAGTGGAGATGTGGGTTATGGAATTATCTGTGAAACTTCTGTTGGAAATGCAGTTATAACAATGTATGGAAAGCATGGCATGATTGAAGAGGCCGAGACAATGTTCTATGCACTGGATGAGAGGAACTTGATCTCTTGGACAGCTGCTCTGGATGTTTTCTTGAAAGTACTTGATCTAGGCATTCATTGTGATTCTAGTTTTTTATGTACTATTCTTGATGCCTGCTCAGAGTGCAGAAATCTGGAATTGGGACGTCAAATCCACGCATGTGTTACAAAACTTGGTTATTACTCTGGTGTTCATGTTGGGACAGCTTTGATAGACATATATGCCAAATGTGGGAACTTTCAATCTGCAAGACGGGTTTTCGATGGTCTTTCAGGTAAAAATACTGCCTCACTCAATGCAGTTCTGGCTGGATTCATGGAATCACATATACATGTTGAAGATGATCCCATTGTCTTATTTAATCAATCAAGATATGCTGGTATAATTCCAGATTCCATAACTTTTTCACGGCTCCTTAGCTTGTCTGCTGAGGAAGCCTGCTTGGTTATGGGTAAGAGTCTTCATTCTTATGCTATCAAAGCTGGACTTGAAGCCAATTCCACTGTAGGTAATGCTATAATTACCATGTATGCCAAATGTGGTAGCATTGAAGAAGCTTTTCAAATGTTCAATGGTATGAATAGTCGCGATTGTGTATCCTGGAATGCCATAATTTCAGCATATGCTCTTCATGGAGAAGGCACCAAAGCACTTTCACTGTTTGAAAATATGAAGGAAGAAGCTTGCAGTTACTCTGGATTATGGGAAACTGGGTTATGCTTATTCAACGAAATGGAGCCAAAATATGGGACTAGGTCCGTGATTGAGCACTTTGGTTGCATGGTCGATCTTTTGGGTCGGGCTGGGAAATTTTCAGAAGCCATCGATTTCATCAACACAAGTCCATTTCCAGACTCACCGATTCTTTGGAGAACTTTAGTCAATGTCTGCATGTTATGTGGGAATTTAAATTTTGGCATTTTAGCTTCAAGGCATTTGCTTGAGTTGGAACCTGATGAGGCTGGGTCATATATACTTGTTTCAAATATATATGCTGGAGGAGGAATGTTTGATGAGGCTGCAAAAGTTAGAACTGTTATGAATGACTTGAAAGTAAACAAAGAAGCAGGTTGCAGCTGGATTGAAGTAGATAATAAGTTTCATTATTTTGTGGCAAGTGATATGGACCATCCGAAAAGCGATGAAATTTATCAGAAGTTGAATCTCTTGAAGATTCAAATAAAACTGAACAGTCATGATCGAAGTGATCTCTGTCTGGCTGCAGATCCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

723

Amino Acids

80.13

Weight (kDa)

5.32

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 230 - 276 5.6e-08 PPR repeat family
PPR_2 PF13041 509 - 542 4.9e-06 PPR repeat family
PPR PF01535 511 - 539 1.3e-06 PPR repeat
E_motif PF20431 614 - 674 2.4e-14 E motif
Eplus_motif PF20430 680 - 699 9.1e-06 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g23520
malus_domestica MD12G1072400.v1.1 MD14G1069600.v1.1
pyrus_communis pycom12g06080 pycom14g05770
rosa_chinensis RchiOBHm_Chr3g0481521
rosa_laevigata RLG00000023390
rosa_multiflora Rmu_co8224982.1_g000001 Rmu_sc0010846.1_g000006
rosa_roxburghii Rroxscaffold_6G00400370
rosa_rugosa Rorug03G0192400
rosa_samantha Rh3AG243600 Rh3BG278500 Rh3CG273700 Rh3DG270500
rosa_wichuraiana Rw3G021760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 884, 2170
AatII GACGTC 1 cut(s) 1084
Acc36I ACCTGC 1 cut(s) 1994
AccB1I GGYRCC 2 cut(s) 276, 1577
AccB7I CCANNNNNTGG 1 cut(s) 1682
AccBSI CCGCTC 1 cut(s) 35
AccII CGCG 1 cut(s) 1524
AciI CCGC 1 cut(s) 33
AclI AACGTT 1 cut(s) 597
AclWI GGATC 3 cut(s) 782, 1274, 2156
AcsI RAATTY 6 cut(s) 209, 332, 1745, 1840, 1846, 2082
AcuI CTGAAG 1 cut(s) 572
AcyI GRCGYC 1 cut(s) 1081
AfaI GTAC 5 cut(s) 45, 326, 629, 996, 1036
AfiI CCNNNNNNNGG 2 cut(s) 1167, 1682
AflIII ACRYGT 3 cut(s) 321, 465, 1267
AjiI CACGTC 1 cut(s) 548
AjnI CCWGG 1 cut(s) 1535
AjuI GAANNNNNNNTTGG 2 cut(s) 852, 884
AleI CACNNNNGTG 1 cut(s) 1095
Alw21I GWGCWC 1 cut(s) 1707
Alw26I GTCTC 2 cut(s) 625, 917
AlwI GGATC 3 cut(s) 782, 1274, 2156
Ama87I CYCGRG 1 cut(s) 459
AoxI GGCC 5 cut(s) 150, 194, 729, 733, 918
ApeKI GCWGC 5 cut(s) 174, 971, 1955, 2010, 2156
ApoI RAATTY 6 cut(s) 209, 332, 1745, 1840, 1846, 2082
ArsI GACNNNNNNTTYG 4 cut(s) 101, 133, 584, 616
Asp700I GAANNNNTTC 3 cut(s) 558, 665, 1494
AspS9I GGNCC 4 cut(s) 222, 729, 1692, 2062
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 1 cut(s) 1785
AvaI CYCGRG 1 cut(s) 459
AvaII GGWCC 3 cut(s) 222, 1692, 2062
BanI GGYRCC 2 cut(s) 276, 1577
BbsI GAAGAC 1 cut(s) 1388
Bbv12I GWGCWC 1 cut(s) 1707
BbvCI CCTCAGC 2 cut(s) 198, 1365
BbvI GCAGC 5 cut(s) 186, 958, 1942, 2022, 2143
BccI CCATC 4 cut(s) 86, 1193, 1766, 2073
BceAI ACGGC 1 cut(s) 1361
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 1 cut(s) 1537
BciVI GTATCC 1 cut(s) 1543
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 2 cut(s) 625, 917
BfaI CTAG 5 cut(s) 533, 633, 1004, 1023, 1689
BfmI CTRYAG 2 cut(s) 1440, 2157
BfuAI ACCTGC 1 cut(s) 1994
BfuI GTATCC 1 cut(s) 1543
BisI GCNGC 5 cut(s) 175, 972, 1956, 2011, 2157
BlsI GCNGC 5 cut(s) 176, 973, 1957, 2012, 2158
BmcAI AGTACT 1 cut(s) 996
Bme1390I CCNGG 2 cut(s) 359, 1537
Bme18I GGWCC 3 cut(s) 222, 1692, 2062
BmeT110I CYCGRG 1 cut(s) 459
BmgBI CACGTC 1 cut(s) 548
BmgT120I GGNCC 4 cut(s) 222, 729, 1692, 2062
BmiI GGNNCC 6 cut(s) 223, 278, 1349, 1579, 1674, 1887
BmrFI CCNGG 2 cut(s) 359, 1537
BmrI ACTGGG 1 cut(s) 1656
BmsI GCATC 4 cut(s) 225, 340, 449, 1036
BmuI ACTGGG 1 cut(s) 1656
BpiI GAAGAC 1 cut(s) 1388
BpmI CTGGAG 1 cut(s) 1953
Bpu10I CCTNAGC 3 cut(s) 198, 1352, 1365
BpuEI CTTGAG 1 cut(s) 1898
BpuMI CCSGG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 1761
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 1081
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 1 cut(s) 736
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 1167, 1682
Bse118I RCCGGY 2 cut(s) 273, 731
Bse1I ACTGG 3 cut(s) 694, 945, 1651
Bse3DI GCAATG 2 cut(s) 453, 658
BseBI CCWGG 1 cut(s) 1537
BseCI ATCGAT 1 cut(s) 1761
BseDI CCNNGG 1 cut(s) 736
BseGI GGATG 4 cut(s) 352, 949, 985, 2065
BseLI CCNNNNNNNGG 2 cut(s) 1167, 1682
BseMI GCAATG 2 cut(s) 453, 658
BseMII CTCAG 6 cut(s) 212, 369, 414, 764, 1068, 1356
BseNI ACTGG 3 cut(s) 694, 945, 1651
BseRI GAGGAG 3 cut(s) 194, 494, 1950
BseXI GCAGC 5 cut(s) 186, 958, 1942, 2022, 2143
BseYI CCCAGC 3 cut(s) 238, 1739, 1898
BsgI GTGCAG 1 cut(s) 1081
Bsh1236I CGCG 1 cut(s) 1524
BshFI GGCC 5 cut(s) 152, 196, 731, 735, 920
BshNI GGYRCC 2 cut(s) 276, 1577
BshVI ATCGAT 1 cut(s) 1761
BsiHKAI GWGCWC 1 cut(s) 1707
BsiHKCI CYCGRG 1 cut(s) 459
BsiSI CCGG 4 cut(s) 149, 274, 358, 732
BslFI GGGAC 4 cut(s) 476, 1092, 1150, 1699
BslI CCNNNNNNNGG 2 cut(s) 1167, 1682
BsmAI GTCTC 2 cut(s) 625, 917
BsmFI GGGAC 4 cut(s) 476, 1092, 1150, 1699
BsmI GAATGC 2 cut(s) 1008, 1546
BsnI GGCC 5 cut(s) 152, 196, 731, 735, 920
Bso31I GGTCTC 1 cut(s) 625
BsoBI CYCGRG 1 cut(s) 459
Bsp1286I GDGCHC 1 cut(s) 1707
Bsp143I GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
Bsp68I TCGCGA 1 cut(s) 1524
BspACI CCGC 1 cut(s) 33
BspANI GGCC 5 cut(s) 152, 196, 731, 735, 920
BspCNI CTCAG 6 cut(s) 211, 370, 415, 765, 1067, 1357
BspDI ATCGAT 1 cut(s) 1761
BspFNI CGCG 1 cut(s) 1524
BspHI TCATGA 1 cut(s) 2131
BspLI GGNNCC 6 cut(s) 223, 278, 1349, 1579, 1674, 1887
BspMAI CTGCAG 1 cut(s) 2161
BspMI ACCTGC 1 cut(s) 1994
BspPI GGATC 3 cut(s) 782, 1274, 2156
BspQI GCTCTTC 1 cut(s) 1569
BspT107I GGYRCC 2 cut(s) 276, 1577
BspTNI GGTCTC 1 cut(s) 625
BsrBI CCGCTC 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 453, 658
BsrFI RCCGGY 2 cut(s) 273, 731
BsrI ACTGG 3 cut(s) 694, 945, 1651
BssAI RCCGGY 2 cut(s) 273, 731
BssECI CCNNGG 1 cut(s) 736
BssMI GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
BssNI GRCGYC 1 cut(s) 1081
Bst2UI CCWGG 1 cut(s) 1537
Bst4CI ACNGT 4 cut(s) 1441, 1596, 1972, 2129
Bst6I CTCTTC 6 cut(s) 97, 150, 188, 531, 909, 1569
BstACI GRCGYC 1 cut(s) 1081
BstAPI GCANNNNNTGC 1 cut(s) 254
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 5 cut(s) 733, 826, 1051, 1375, 1621
BstDEI CTNAG 7 cut(s) 198, 378, 423, 773, 1054, 1352, 1365
BstF5I GGATG 4 cut(s) 352, 949, 985, 2065
BstFNI CGCG 1 cut(s) 1524
BstKTI GATC 9 cut(s) 769, 790, 960, 1003, 1282, 1726, 2137, 2146, 2164
BstMAI GTCTC 2 cut(s) 625, 917
BstMBI GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
BstMWI GCNNNNNNNGC 5 cut(s) 254, 1059, 1241, 1370, 1415
BstNI CCWGG 1 cut(s) 1537
BstNSI RCATGY 6 cut(s) 325, 469, 828, 1098, 1271, 1831
BstSCI CCNGG 2 cut(s) 357, 1535
BstSFI CTRYAG 2 cut(s) 1440, 2157
BstSNI TACGTA 1 cut(s) 43
BstUI CGCG 1 cut(s) 1524
BstV1I GCAGC 5 cut(s) 186, 958, 1942, 2022, 2143
BstV2I GAAGAC 1 cut(s) 1388
BstX2I RGATCY 2 cut(s) 787, 2161
BstYI RGATCY 2 cut(s) 787, 2161
Bsu15I ATCGAT 1 cut(s) 1761
BsuI GTATCC 1 cut(s) 1543
BsuRI GGCC 5 cut(s) 152, 196, 731, 735, 920
BsuTUI ATCGAT 1 cut(s) 1761
BtgZI GCGATG 1 cut(s) 2091
BtrI CACGTC 1 cut(s) 548
BtsCI GGATG 4 cut(s) 352, 949, 985, 2065
BtsI GCAGTG 1 cut(s) 835
BtsIMutI CAGTG 7 cut(s) 676, 701, 826, 835, 938, 1437, 1592
BtuMI TCGCGA 1 cut(s) 1524
BveI ACCTGC 1 cut(s) 1994
Cac8I GCNNGC 5 cut(s) 733, 826, 1051, 1375, 1621
CciI TCATGA 1 cut(s) 2131
Cfr10I RCCGGY 2 cut(s) 273, 731
Cfr13I GGNCC 4 cut(s) 222, 729, 1692, 2062
ClaI ATCGAT 1 cut(s) 1761
Csp6I GTAC 5 cut(s) 44, 325, 628, 995, 1035
CviQI GTAC 5 cut(s) 44, 325, 628, 995, 1035
DdeI CTNAG 7 cut(s) 198, 378, 423, 773, 1054, 1352, 1365
DpnI GATC 9 cut(s) 768, 789, 959, 1002, 1281, 1725, 2136, 2145, 2163
DpnII GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
DraI TTTAAA 1 cut(s) 1845
Eam1104I CTCTTC 6 cut(s) 97, 150, 188, 531, 909, 1569
EarI CTCTTC 6 cut(s) 97, 150, 188, 531, 909, 1569
Eco105I TACGTA 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 625
Eco32I GATATC 1 cut(s) 691
Eco47I GGWCC 3 cut(s) 222, 1692, 2062
Eco57I CTGAAG 1 cut(s) 572
Eco88I CYCGRG 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 1535
EcoRV GATATC 1 cut(s) 691
EcoT22I ATGCAT 1 cut(s) 442
FalI AAGNNNNNCTT 2 cut(s) 1407, 1439
FaqI GGGAC 4 cut(s) 476, 1092, 1150, 1699
FauI CCCGC 1 cut(s) 40
FauNDI CATATG 1 cut(s) 1558
Fnu4HI GCNGC 5 cut(s) 175, 972, 1956, 2011, 2157
FokI GGATG 4 cut(s) 359, 956, 992, 2052
FseI GGCCGGCC 1 cut(s) 735
Fsp4HI GCNGC 5 cut(s) 175, 972, 1956, 2011, 2157
FspBI CTAG 5 cut(s) 533, 633, 1004, 1023, 1689
GluI GCNGC 5 cut(s) 175, 972, 1956, 2011, 2157
GsaI CCCAGC 3 cut(s) 242, 1743, 1902
GsuI CTGGAG 1 cut(s) 1953
HaeIII GGCC 5 cut(s) 152, 196, 731, 735, 920
HapII CCGG 4 cut(s) 149, 274, 358, 732
Hin1I GRCGYC 1 cut(s) 1081
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 2 cut(s) 1491, 1617
HpaII CCGG 4 cut(s) 149, 274, 358, 732
HphI GGTGA 1 cut(s) 1785
Hpy166II GTNNAC 3 cut(s) 319, 530, 1993
Hpy188I TCNGA 9 cut(s) 101, 234, 424, 740, 774, 1057, 1753, 2070, 2091
Hpy8I GTNNAC 3 cut(s) 319, 530, 1993
HpyAV CCTTC 7 cut(s) 20, 67, 188, 268, 406, 1568, 1603
HpyCH4III ACNGT 4 cut(s) 1441, 1596, 1972, 2129
HpyCH4IV ACGT 4 cut(s) 42, 547, 597, 1081
HpyF10VI GCNNNNNNNGC 5 cut(s) 254, 1059, 1241, 1370, 1415
HpyF3I CTNAG 7 cut(s) 198, 378, 423, 773, 1054, 1352, 1365
HpySE526I ACGT 4 cut(s) 42, 547, 597, 1081
Hsp92I GRCGYC 1 cut(s) 1081
KroI GCCGGC 1 cut(s) 731
KroNI GCCGGC 1 cut(s) 733
Kzo9I GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
LguI GCTCTTC 1 cut(s) 1569
LmnI GCTCC 3 cut(s) 207, 1353, 1672
Lsp1109I GCAGC 5 cut(s) 186, 958, 1942, 2022, 2143
LweI GCATC 4 cut(s) 225, 340, 449, 1036
MaeI CTAG 5 cut(s) 533, 633, 1004, 1023, 1689
MaeII ACGT 4 cut(s) 42, 547, 597, 1081
MaeIII GTNAC 5 cut(s) 117, 638, 671, 1099, 1625
MalI GATC 9 cut(s) 768, 789, 959, 1002, 1281, 1725, 2136, 2145, 2163
MbiI CCGCTC 1 cut(s) 35
MboI GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
MfeI CAATTG 2 cut(s) 216, 750
MflI RGATCY 2 cut(s) 787, 2161
MhlI GDGCHC 1 cut(s) 1707
MlyI GAGTC 3 cut(s) 548, 1402, 1784
MmeI TCCRAC 2 cut(s) 850, 1863
Mph1103I ATGCAT 1 cut(s) 442
MroNI GCCGGC 1 cut(s) 731
MroXI GAANNNNTTC 3 cut(s) 558, 665, 1494
MseI TTAA 5 cut(s) 489, 701, 815, 1296, 1844
MslI CAYNNNNRTG 3 cut(s) 1095, 1266, 1404
MspA1I CMGCKG 2 cut(s) 971, 2013
MspI CCGG 4 cut(s) 149, 274, 358, 732
MspR9I CCNGG 2 cut(s) 359, 1537
MunI CAATTG 2 cut(s) 216, 750
Mva1269I GAATGC 2 cut(s) 1008, 1546
MvaI CCWGG 1 cut(s) 1537
MvnI CGCG 1 cut(s) 1524
MwoI GCNNNNNNNGC 5 cut(s) 254, 1059, 1241, 1370, 1415
NaeI GCCGGC 1 cut(s) 733
NciI CCSGG 1 cut(s) 359
NdeI CATATG 1 cut(s) 1558
NdeII GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
NgoMIV GCCGGC 1 cut(s) 731
NlaIV GGNNCC 6 cut(s) 223, 278, 1349, 1579, 1674, 1887
NmeAIII GCCGAG 2 cut(s) 682, 946
NmuCI GTSAC 3 cut(s) 117, 638, 671
NruI TCGCGA 1 cut(s) 1524
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 6 cut(s) 325, 469, 828, 1098, 1271, 1831
OliI CACNNNNGTG 1 cut(s) 1095
PaeI GCATGC 1 cut(s) 828
PagI TCATGA 1 cut(s) 2131
PciI ACATGT 3 cut(s) 321, 465, 1267
PciSI GCTCTTC 1 cut(s) 1569
PctI GAATGC 2 cut(s) 1008, 1546
PdiI GCCGGC 1 cut(s) 733
PdmI GAANNNNTTC 3 cut(s) 558, 665, 1494
PfeI GAWTC 7 cut(s) 1019, 1248, 1256, 1328, 1798, 2098, 2110
PflFI GACNNNGTC 1 cut(s) 1690
PflMI CCANNNNNTGG 1 cut(s) 1682
PfoI TCCNGGA 1 cut(s) 1535
PkrI GCNGC 5 cut(s) 176, 973, 1957, 2012, 2158
PleI GAGTC 3 cut(s) 547, 1401, 1784
PpsI GAGTC 3 cut(s) 547, 1401, 1784
Ppu21I YACGTR 1 cut(s) 43
PscI ACATGT 3 cut(s) 321, 465, 1267
PsiI TTATAA 2 cut(s) 884, 2170
Psp1406I AACGTT 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 1535
PspFI CCCAGC 3 cut(s) 238, 1739, 1898
PspGI CCWGG 1 cut(s) 1535
PspN4I GGNNCC 6 cut(s) 223, 278, 1349, 1579, 1674, 1887
PspPI GGNCC 4 cut(s) 222, 729, 1692, 2062
PstI CTGCAG 1 cut(s) 2161
PsuI RGATCY 2 cut(s) 787, 2161
PsyI GACNNNGTC 1 cut(s) 1690
PvuII CAGCTG 2 cut(s) 971, 2013
RigI GGCCGGCC 1 cut(s) 735
RruI TCGCGA 1 cut(s) 1524
RsaI GTAC 5 cut(s) 45, 326, 629, 996, 1036
RsaNI GTAC 5 cut(s) 44, 325, 628, 995, 1035
RseI CAYNNNNRTG 3 cut(s) 1095, 1266, 1404
SapI GCTCTTC 1 cut(s) 1569
SaqAI TTAA 5 cut(s) 489, 701, 815, 1296, 1844
SatI GCNGC 5 cut(s) 175, 972, 1956, 2011, 2157
Sau3AI GATC 9 cut(s) 766, 787, 957, 1000, 1279, 1723, 2134, 2143, 2161
Sau96I GGNCC 4 cut(s) 222, 729, 1692, 2062
ScaI AGTACT 1 cut(s) 996
SchI GAGTC 3 cut(s) 548, 1402, 1784
ScrFI CCNGG 2 cut(s) 359, 1537
SduI GDGCHC 1 cut(s) 1707
SfaNI GCATC 4 cut(s) 225, 340, 449, 1036
SfcI CTRYAG 2 cut(s) 1440, 2157
SinI GGWCC 3 cut(s) 222, 1692, 2062
SmiI ATTTAAAT 1 cut(s) 1845
SmiMI CAYNNNNRTG 3 cut(s) 1095, 1266, 1404
SmlI CTYRAG 1 cut(s) 1877
SmoI CTYRAG 1 cut(s) 1877
SnaBI TACGTA 1 cut(s) 43
SphI GCATGC 1 cut(s) 828
SsiI CCGC 1 cut(s) 33
SspMI CTAG 5 cut(s) 533, 633, 1004, 1023, 1689
StyD4I CCNGG 2 cut(s) 357, 1535
SwaI ATTTAAAT 1 cut(s) 1845
TaaI ACNGT 4 cut(s) 1441, 1596, 1972, 2129
TaiI ACGT 4 cut(s) 45, 550, 600, 1084
TaqI TCGA 6 cut(s) 329, 366, 1197, 1722, 1761, 2137
TatI WGTACW 4 cut(s) 324, 627, 994, 1034
TfiI GAWTC 7 cut(s) 1019, 1248, 1256, 1328, 1798, 2098, 2110
Tru1I TTAA 5 cut(s) 489, 701, 815, 1296, 1844
Tru9I TTAA 5 cut(s) 489, 701, 815, 1296, 1844
TscAI CASTG 7 cut(s) 676, 701, 826, 835, 945, 1444, 1599
TseFI GTSAC 3 cut(s) 117, 638, 671
TseI GCWGC 5 cut(s) 174, 971, 1955, 2010, 2156
Tsp45I GTSAC 3 cut(s) 117, 638, 671
TspGWI ACGGA 1 cut(s) 1684
TspRI CASTG 7 cut(s) 676, 701, 826, 835, 945, 1444, 1599
Tth111I GACNNNGTC 1 cut(s) 1690
Van91I CCANNNNNTGG 1 cut(s) 1682
VpaK11BI GGWCC 3 cut(s) 222, 1692, 2062
XapI RAATTY 6 cut(s) 209, 332, 1745, 1840, 1846, 2082
XbaI TCTAGA 1 cut(s) 632
XceI RCATGY 6 cut(s) 325, 469, 828, 1098, 1271, 1831
XmnI GAANNNNTTC 3 cut(s) 558, 665, 1494
XspI CTAG 5 cut(s) 533, 633, 1004, 1023, 1689
ZraI GACGTC 1 cut(s) 1082
ZrmI AGTACT 1 cut(s) 996
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.