pycom14g05770

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015659
Physical Location & Seq
Reverse (-)
5612287 .. 5612892
606 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05770.1

Sequence Viewer

Length: 606 bp
ATGGAACCACATAGAGATGTTGAGGAGGATTCCATGGTCTTATTTAGTCAGTTAAGATCTTCTGGTATGCATCCAGATTTCATAACGTATTCACGGCTCCTTAGTGTAGCAGCTGAAGAAGCTTGCTTAGAAAGGGGGAAGAGTCTCCATGCTTGCATTATTAAAGTTGGATTTGAAGCCAATTCCACTGCAGCTAATGCTGTAATTACCATGTATGCCAAATGTGGTAGCATTGGAGAAGCTTATCAAATGTTCAATGGTATGAATTGTCGTGATTGCATATCCTGGAACGCCATAATTTCGGCGCATGCCCTTCATGGAGAAGGCAACAAAGCACTTTCACTTTTGAAATCAATGAAGGAAGAAGGATTTGCCCCAGATGAGATCACATTATTGGCTACTCTTCAAGCTTGCAGGTACGCTGGACTATGGGAAACTGGGTTTCGCTTATTCAACGAAATGGTGCCAAAGTATGGAACTAAGTCAGGAAATGAGCACTTTGTATGCACGGTGGATCTTCTGGGTCGGTCTGGGAATTTTCAGAAGCCATCGATTTCATCAACAAAAGTCCATTTCCAGACTCACCTATGCTTTGGAGAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

202

Amino Acids

22.18

Weight (kDa)

6.05

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 92 - 137 8.6e-09 PPR repeat family
PPR PF01535 94 - 124 5.1e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g23520
malus_domestica MD12G1072400.v1.1 MD14G1069600.v1.1
pyrus_communis pycom12g06080 pycom14g05770
rosa_chinensis RchiOBHm_Chr3g0481521
rosa_laevigata RLG00000023390
rosa_multiflora Rmu_co8224982.1_g000001 Rmu_sc0010846.1_g000006
rosa_roxburghii Rroxscaffold_6G00400370
rosa_rugosa Rorug03G0192400
rosa_samantha Rh3AG243600 Rh3BG278500 Rh3CG273700 Rh3DG270500
rosa_wichuraiana Rw3G021760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 405
AccB1I GGYRCC 1 cut(s) 463
AccB7I CCANNNNNTGG 1 cut(s) 473
AclWI GGATC 1 cut(s) 522
AcsI RAATTY 1 cut(s) 535
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 5 cut(s) 176, 256, 349, 407, 454
AjnI CCWGG 1 cut(s) 284
AluBI AGCT 5 cut(s) 113, 122, 194, 242, 410
AluI AGCT 5 cut(s) 113, 122, 194, 242, 410
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 522
ApeKI GCWGC 2 cut(s) 110, 191
ApoI RAATTY 1 cut(s) 535
AspLEI GCGC 1 cut(s) 307
AsuHPI GGTGA 1 cut(s) 575
BanI GGYRCC 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 2 cut(s) 122, 203
BccI CCATC 1 cut(s) 556
BceAI ACGGC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 405
BglII AGATCT 1 cut(s) 56
BisI GCNGC 2 cut(s) 111, 192
BlsI GCNGC 2 cut(s) 112, 193
Bme1390I CCNGG 1 cut(s) 286
BmiI GGNNCC 3 cut(s) 6, 98, 465
BmrFI CCNGG 1 cut(s) 286
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 447
Bsa29I ATCGAT 1 cut(s) 551
BsaJI CCNNGG 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 442
BseBI CCWGG 1 cut(s) 286
BseCI ATCGAT 1 cut(s) 551
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 1 cut(s) 70
BseLI CCNNNNNNNGG 1 cut(s) 473
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 2 cut(s) 122, 203
BshNI GGYRCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 551
BsiHKAI GWGCWC 1 cut(s) 498
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 3 cut(s) 56, 384, 514
Bsp19I CCATGG 1 cut(s) 33
BspDI ATCGAT 1 cut(s) 551
BspLI GGNNCC 3 cut(s) 6, 98, 465
BspMAI CTGCAG 1 cut(s) 193
BspMI ACCTGC 1 cut(s) 405
BspPI GGATC 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 463
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 3 cut(s) 56, 384, 514
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 2 cut(s) 134, 408
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 4 cut(s) 124, 154, 309, 412
BstDEI CTNAG 3 cut(s) 101, 127, 480
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 70
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 3 cut(s) 59, 387, 517
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 3 cut(s) 56, 384, 514
BstMWI GCNNNNNNNGC 2 cut(s) 119, 197
BstNI CCWGG 1 cut(s) 286
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 284
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 122, 203
BstX2I RGATCY 2 cut(s) 56, 514
BstYI RGATCY 2 cut(s) 56, 514
Bsu15I ATCGAT 1 cut(s) 551
BsuTUI ATCGAT 1 cut(s) 551
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 186
BveI ACCTGC 1 cut(s) 405
Cac8I GCNNGC 4 cut(s) 124, 154, 309, 412
CfoI GCGC 1 cut(s) 307
ClaI ATCGAT 1 cut(s) 551
Csp6I GTAC 1 cut(s) 418
CviAII CATG 5 cut(s) 34, 149, 211, 308, 317
CviJI RGCY 9 cut(s) 97, 113, 122, 179, 194, 242, 398, 410, 547
CviKI_1 RGCY 9 cut(s) 97, 113, 122, 179, 194, 242, 398, 410, 547
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 3 cut(s) 101, 127, 480
DpnI GATC 3 cut(s) 58, 386, 516
DpnII GATC 3 cut(s) 56, 384, 514
Eam1104I CTCTTC 2 cut(s) 134, 408
EarI CTCTTC 2 cut(s) 134, 408
Eco130I CCWWGG 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 5 cut(s) 37, 152, 214, 311, 320
FatI CATG 5 cut(s) 33, 148, 210, 307, 316
Fnu4HI GCNGC 2 cut(s) 111, 192
FokI GGATG 1 cut(s) 57
Fsp4HI GCNGC 2 cut(s) 111, 192
GlaI GCGC 1 cut(s) 306
GluI GCNGC 2 cut(s) 111, 192
HhaI GCGC 1 cut(s) 307
Hin1II CATG 5 cut(s) 37, 152, 214, 311, 320
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 3 cut(s) 120, 240, 408
HinfI GANTC 3 cut(s) 29, 142, 580
HphI GGTGA 1 cut(s) 575
Hpy188I TCNGA 1 cut(s) 543
Hpy188III TCNNGA 4 cut(s) 74, 272, 486, 577
HpyAV CCTTC 4 cut(s) 317, 323, 352, 359
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 6 cut(s) 70, 156, 191, 279, 414, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 197
HpyF3I CTNAG 3 cut(s) 101, 127, 480
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 5 cut(s) 37, 152, 214, 311, 320
HspAI GCGC 1 cut(s) 305
Kzo9I GATC 3 cut(s) 56, 384, 514
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 2 cut(s) 122, 203
LweI GCATC 1 cut(s) 79
MaeII ACGT 1 cut(s) 86
MalI GATC 3 cut(s) 58, 386, 516
MboI GATC 3 cut(s) 56, 384, 514
MboII GAAGA 6 cut(s) 51, 128, 151, 374, 395, 509
MflI RGATCY 2 cut(s) 56, 514
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 5 cut(s) 181, 204, 265, 297, 535
MlyI GAGTC 2 cut(s) 151, 574
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 2 cut(s) 16, 19
Mph1103I ATGCAT 1 cut(s) 72
MseI TTAA 2 cut(s) 53, 162
MslI CAYNNNNRTG 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 286
MwoI GCNNNNNNNGC 2 cut(s) 119, 197
NcoI CCATGG 1 cut(s) 33
NdeII GATC 3 cut(s) 56, 384, 514
NlaIII CATG 5 cut(s) 37, 152, 214, 311, 320
NlaIV GGNNCC 3 cut(s) 6, 98, 465
NsiI ATGCAT 1 cut(s) 72
NspI RCATGY 1 cut(s) 311
PaeI GCATGC 1 cut(s) 311
PfeI GAWTC 1 cut(s) 29
PflMI CCANNNNNTGG 1 cut(s) 473
PfoI TCCNGGA 1 cut(s) 284
PkrI GCNGC 2 cut(s) 112, 193
PleI GAGTC 2 cut(s) 150, 574
PpsI GAGTC 2 cut(s) 150, 574
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 3 cut(s) 6, 98, 465
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 2 cut(s) 56, 514
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 2 cut(s) 53, 162
SatI GCNGC 2 cut(s) 111, 192
Sau3AI GATC 3 cut(s) 56, 384, 514
SchI GAGTC 2 cut(s) 151, 574
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 1 cut(s) 498
SetI ASST 8 cut(s) 89, 115, 124, 196, 244, 412, 419, 588
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 15
SphI GCATGC 1 cut(s) 311
Sse9I AATT 5 cut(s) 181, 204, 265, 297, 535
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 89
TaqI TCGA 1 cut(s) 551
TaqII GACCGA 1 cut(s) 516
TasI AATT 5 cut(s) 181, 204, 265, 297, 535
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 2 cut(s) 53, 162
Tru9I TTAA 2 cut(s) 53, 162
TscAI CASTG 1 cut(s) 193
TseI GCWGC 2 cut(s) 110, 191
TspDTI ATGAA 5 cut(s) 70, 278, 305, 371, 546
TspRI CASTG 1 cut(s) 193
Van91I CCANNNNNTGG 1 cut(s) 473
XapI RAATTY 1 cut(s) 535
XceI RCATGY 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.