Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006334

Physical Location & Seq

Genomic Coordinates Reverse (-)

12294080 .. 12307377

Gene Structure 13298 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1093700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGCCGCTTCCTCTTCTCTCTCTTTTAGGTCTTTCTTCAGCTCCGTCGCCGCCGATTCCAATGACGTCGGTTTAACGCCTGGGAATTTCGGGCCAAGCTTTGTGGATTCCCAGGTCAGTTTCAGGTTCGCAAAGGCAACGATATCAACGGGAAAGACGTTGTCCTGTGAGAATAAATTTCAATTGAAGTGTTCGGTGGACAGAGATATGGATCTGAGCACTTCAGCAATATCTGATGGTGTTGCAGACTGCGTAAATGAAGTTGGGCTGAAAGAGCCAAGTATCTCAACCATGCTGATGAACTTTGAGAACAAGTTTGATCCGTTTGATGCAATGAATACGCCACTTTACCAAACAGCTACGTTTAAGCAGCCTTCAGCAACAGAAAATGGTGCTTATGATTATACTAGAAGTGGAAATCCCACACGGGATGCCTTGGAAAGCCTTTTGGCAAAGCTTGATAAAGCAGATCGAGCATTTTGTTTCACTAGTGGAATGGCTGCTTTGGCGGCTGTTGCTCATCTTTTAGGAACAGGTGAGGAGATTGTTGCAGGAGACGACATTTATGGAGGTTCTGATCGGTTGCTATCACAAGTAGTTCCAAAGACTGGAGTTGTGGTGAAACGAGTGAACACAAGTGATTTAGATGAGGTTGCATCTGCTATTGGCCCCTGGACAAAGCTTGTGTGGCTGGAGAGTCCCACCAATCCTCGACAAATGATTTCTGATATTCGTAAAATAGCAGAGATGGCTCATGCACATGGTGCTATTGTGTTAGTCGATAACAGTATAATGTCTCCCGTATTGTCACAGCCACTGGATCTCGGAGCAGACATTGTTATGCACTCGGCCACAAAGTTTATTGCTGGACACAGTGATGTCATGGCAGGTGTTTTAGCTGTTAAAGGCGATAGCTTGGCGAAACAGCTATATTTCCTACAAAATGCAGAAGGCTCTGGGTTAGCTCCATTTGATTGTTGGGTCTGTTTACGAGGAATTAAGACAATGGCCTTGCGTGTTGAGAAGCAACAGGATAATGCTCAAAAAATTGCTGAGTTCCTCGCTTCCCATCCGCGAGTGACACAAGTTAATTATGCTGGGCTTTCTGGTCATCCTGGACGTGCCTTGCATTATTCTCAGGCAAAGGGTGCAGGATCTGTATTGAGTTTTCTGACAGGTTCATTGTCACTATCAAAGCATATTGTCGAGACTACCAAATACTTCAGTATAACTGTCAGTTTCGGGAGTGTGAAGTCCCTTATAAGCCTGCCGTGCTTCATGTCTCATGCAAGCATACCATCTGCAGTGCGTGAGGCCAGAGGTCTATCTGAAGATCTTGTTCGTATTTCTGTGGGGATTGAGGATGTGAATGATCTGATTGCTGATTTGGACCATGCACTTAAAACTGGCGCTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003962 GO:0004121 GO:0004123 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006534 GO:0006555 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009069 GO:0009070 GO:0009086 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016765 GO:0016829 GO:0016846 GO:0017144 GO:0019279 GO:0019343 GO:0019344 GO:0019346 GO:0019752 GO:0019842 GO:0030170 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048037 GO:0050662 GO:0050667 GO:0070279 GO:0071265 GO:0071266 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Cys_Met_Meta_PP

Protein Analysis

473

Amino Acids

50.35

Weight (kDa)

5.68

Isoelectric Point (pI)

29.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cys_Met_Meta_PP	PF01053	97 - 468	9.6e-145	Cys/Met metabolism PLP-dependent enzyme
Aminotran_1_2	PF00155	135 - 262	6e-06	Aminotransferase class I and II
Aminotran_5	PF00266	168 - 289	9.3e-07	Aminotransferase class-V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006334)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57050 AT3G57050 AT3G57050 AT3G57050 AT3G57050
fragaria_vesca	FvH4_3g30150 FvH4_3g30150 FvH4_3g30150 FvH4_3g30150 FvH4_3g30150
malus_domestica	MD12G1093700.v1.1
prunus_persica	Prupe.7G042400_v2.0.a1 Prupe.7G042400_v2.0.a1 Prupe.7G042400_v2.0.a1
pyrus_communis	pycom12g07890
rosa_chinensis	RchiOBHm_Chr2g0125281
rosa_laevigata	RLG00000018853
rosa_multiflora	Rmu_sc0003505.1_g000036
rosa_roxburghii	Rroxscaffold_2G00118120 Rroxscaffold_2G00118630
rosa_rugosa	Rorug02G0256500 Rorug02G0256600
rosa_samantha	Rh2AG314800 Rh2BG322900 Rh2CG304900 Rh2DG341100
rosa_wichuraiana	Rw2G025440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1262
AarI	CACCTGC	1 cut(s)	878
AatII	GACGTC	1 cut(s)	69
Acc36I	ACCTGC	1 cut(s)	878
AccB7I	CCANNNNNTGG	1 cut(s)	608
AccII	CGCG	1 cut(s)	1075
AciI	CCGC	4 cut(s)	6, 51, 509, 1073
AclWI	GGATC	4 cut(s)	219, 314, 828, 1162
AcoI	YGGCCR	2 cut(s)	3, 849
AcsI	RAATTY	2 cut(s)	85, 176
AcuI	CTGAAG	5 cut(s)	22, 207, 360, 1207, 1350
AcyI	GRCGYC	1 cut(s)	66
AdeI	CACNNNGTG	1 cut(s)	764
AfiI	CCNNNNNNNGG	1 cut(s)	608
AgsI	TTSAA	2 cut(s)	182, 187
AhlI	ACTAGT	1 cut(s)	488
AjiI	CACGTC	1 cut(s)	1121
AjnI	CCWGG	4 cut(s)	79, 111, 671, 1114
AluBI	AGCT	9 cut(s)	42, 99, 359, 457, 682, 899, 915, 928, 965
AluI	AGCT	9 cut(s)	42, 99, 359, 457, 682, 899, 915, 928, 965
Alw21I	GWGCWC	1 cut(s)	221
Alw26I	GTCTC	4 cut(s)	549, 801, 1202, 1287
AlwI	GGATC	4 cut(s)	219, 314, 828, 1162
AlwNI	CAGNNNCTG	2 cut(s)	817, 1157
AoxI	GGCC	6 cut(s)	3, 92, 667, 849, 1008, 1314
ApeKI	GCWGC	2 cut(s)	370, 500
ApoI	RAATTY	2 cut(s)	85, 176
AspLEI	GCGC	1 cut(s)	1412
AspS9I	GGNCC	3 cut(s)	92, 668, 1390
AsuHPI	GGTGA	2 cut(s)	548, 631
AvaII	GGWCC	1 cut(s)	1390
Bbv12I	GWGCWC	1 cut(s)	221
BbvI	GCAGC	2 cut(s)	382, 487
BccI	CCATC	4 cut(s)	230, 742, 1077, 1306
BceAI	ACGGC	1 cut(s)	1255
BciT130I	CCWGG	4 cut(s)	81, 113, 673, 1116
BcoDI	GTCTC	4 cut(s)	549, 801, 1202, 1287
BcuI	ACTAGT	1 cut(s)	488
BfaI	CTAG	2 cut(s)	408, 489
BfmI	CTRYAG	2 cut(s)	1302, 1415
BfoI	RGCGCY	1 cut(s)	1413
BfuAI	ACCTGC	1 cut(s)	878
BglII	AGATCT	1 cut(s)	1333
BisI	GCNGC	5 cut(s)	6, 51, 371, 501, 510
BlsI	GCNGC	5 cut(s)	7, 52, 372, 502, 511
Bme1390I	CCNGG	4 cut(s)	81, 113, 673, 1116
Bme18I	GGWCC	1 cut(s)	1390
BmgBI	CACGTC	1 cut(s)	1121
BmgT120I	GGNCC	3 cut(s)	92, 668, 1390
BmiI	GGNNCC	1 cut(s)	670
BmrFI	CCNGG	4 cut(s)	81, 113, 673, 1116
BmsI	GCATC	3 cut(s)	319, 421, 665
BpmI	CTGGAG	2 cut(s)	630, 713
BsaBI	GATNNNNATC	1 cut(s)	210
BsaHI	GRCGYC	1 cut(s)	66
BsaJI	CCNNGG	4 cut(s)	80, 111, 435, 671
BsaXI	ACNNNNNCTCC	2 cut(s)	603, 633
Bsc4I	CCNNNNNNNGG	1 cut(s)	608
Bse1I	ACTGG	3 cut(s)	613, 822, 1411
Bse3DI	GCAATG	1 cut(s)	339
Bse8I	GATNNNNATC	1 cut(s)	210
BseBI	CCWGG	4 cut(s)	81, 113, 673, 1116
BseDI	CCNNGG	4 cut(s)	80, 111, 435, 671
BseGI	GGATG	4 cut(s)	436, 1069, 1111, 1369
BseJI	GATNNNNATC	1 cut(s)	210
BseLI	CCNNNNNNNGG	1 cut(s)	608
BseMI	GCAATG	1 cut(s)	339
BseMII	CTCAG	3 cut(s)	206, 1044, 1151
BseNI	ACTGG	3 cut(s)	613, 822, 1411
BseRI	GAGGAG	1 cut(s)	554
BseXI	GCAGC	2 cut(s)	382, 487
BseYI	CCCAGC	1 cut(s)	1097
BsgI	GTGCAG	1 cut(s)	1170
Bsh1236I	CGCG	1 cut(s)	1075
BshFI	GGCC	6 cut(s)	5, 94, 669, 851, 1010, 1316
BsiHKAI	GWGCWC	1 cut(s)	221
BslFI	GGGAC	2 cut(s)	684, 1240
BslI	CCNNNNNNNGG	1 cut(s)	608
BsmAI	GTCTC	4 cut(s)	549, 801, 1202, 1287
BsmBI	CGTCTC	1 cut(s)	549
BsmFI	GGGAC	2 cut(s)	684, 1240
BsnI	GGCC	6 cut(s)	5, 94, 669, 851, 1010, 1316
Bsp1286I	GDGCHC	1 cut(s)	221
Bsp143I	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
BspACI	CCGC	4 cut(s)	6, 51, 509, 1073
BspANI	GGCC	6 cut(s)	5, 94, 669, 851, 1010, 1316
BspCNI	CTCAG	3 cut(s)	207, 1045, 1150
BspFNI	CGCG	1 cut(s)	1075
BspLI	GGNNCC	1 cut(s)	670
BspMAI	CTGCAG	1 cut(s)	1306
BspMI	ACCTGC	1 cut(s)	878
BspPI	GGATC	4 cut(s)	219, 314, 828, 1162
BsrDI	GCAATG	1 cut(s)	339
BsrI	ACTGG	3 cut(s)	613, 822, 1411
BssECI	CCNNGG	4 cut(s)	80, 111, 435, 671
BssMI	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
BssNI	GRCGYC	1 cut(s)	66
BssT1I	CCWWGG	1 cut(s)	435
Bst2UI	CCWGG	4 cut(s)	81, 113, 673, 1116
Bst4CI	ACNGT	3 cut(s)	788, 875, 1234
Bst6I	CTCTTC	1 cut(s)	19
BstACI	GRCGYC	1 cut(s)	66
BstAPI	GCANNNNNTGC	2 cut(s)	764, 1148
BstC8I	GCNNGC	2 cut(s)	1268, 1291
BstDEI	CTNAG	3 cut(s)	215, 1053, 1137
BstF5I	GGATG	4 cut(s)	436, 1069, 1111, 1369
BstFNI	CGCG	1 cut(s)	1075
BstH2I	RGCGCY	1 cut(s)	1413
BstHHI	GCGC	1 cut(s)	1412
BstKTI	GATC	8 cut(s)	214, 322, 472, 580, 823, 1157, 1336, 1375
BstMAI	GTCTC	4 cut(s)	549, 801, 1202, 1287
BstMBI	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
BstNI	CCWGG	4 cut(s)	81, 113, 673, 1116
BstSCI	CCNGG	4 cut(s)	79, 111, 671, 1114
BstSFI	CTRYAG	2 cut(s)	1302, 1415
BstUI	CGCG	1 cut(s)	1075
BstV1I	GCAGC	2 cut(s)	382, 487
BstX2I	RGATCY	4 cut(s)	211, 820, 1154, 1333
BstYI	RGATCY	4 cut(s)	211, 820, 1154, 1333
BsuRI	GGCC	6 cut(s)	5, 94, 669, 851, 1010, 1316
BtrI	CACGTC	1 cut(s)	1121
BtsCI	GGATG	4 cut(s)	436, 1069, 1111, 1369
BtsI	GCAGTG	1 cut(s)	1311
BtsIMutI	CAGTG	3 cut(s)	815, 880, 1311
BveI	ACCTGC	1 cut(s)	878
Cac8I	GCNNGC	2 cut(s)	1268, 1291
CaiI	CAGNNNCTG	2 cut(s)	817, 1157
CfoI	GCGC	1 cut(s)	1412
Cfr13I	GGNCC	3 cut(s)	92, 668, 1390
CspCI	CAANNNNNGTGG	2 cut(s)	84, 119
CviAII	CATG	7 cut(s)	292, 755, 761, 883, 1279, 1286, 1394
DdeI	CTNAG	3 cut(s)	215, 1053, 1137
DpnI	GATC	8 cut(s)	213, 321, 471, 579, 822, 1156, 1335, 1374
DpnII	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
DraIII	CACNNNGTG	1 cut(s)	764
EaeI	YGGCCR	2 cut(s)	3, 849
Eam1104I	CTCTTC	1 cut(s)	19
EarI	CTCTTC	1 cut(s)	19
Eco130I	CCWWGG	1 cut(s)	435
Eco32I	GATATC	1 cut(s)	144
Eco47I	GGWCC	1 cut(s)	1390
Eco57I	CTGAAG	5 cut(s)	22, 207, 360, 1207, 1350
EcoRII	CCWGG	4 cut(s)	79, 111, 671, 1114
EcoRV	GATATC	1 cut(s)	144
EcoT14I	CCWWGG	1 cut(s)	435
ErhI	CCWWGG	1 cut(s)	435
Esp3I	CGTCTC	1 cut(s)	549
FaeI	CATG	7 cut(s)	295, 758, 764, 886, 1282, 1289, 1397
FaqI	GGGAC	2 cut(s)	684, 1240
FatI	CATG	7 cut(s)	291, 754, 760, 882, 1278, 1285, 1393
Fnu4HI	GCNGC	5 cut(s)	6, 51, 371, 501, 510
FokI	GGATG	4 cut(s)	443, 1056, 1098, 1376
Fsp4HI	GCNGC	5 cut(s)	6, 51, 371, 501, 510
FspBI	CTAG	2 cut(s)	408, 489
GlaI	GCGC	1 cut(s)	1411
GluI	GCNGC	5 cut(s)	6, 51, 371, 501, 510
GsaI	CCCAGC	1 cut(s)	1101
GsuI	CTGGAG	2 cut(s)	630, 713
HaeII	RGCGCY	1 cut(s)	1413
HaeIII	GGCC	6 cut(s)	5, 94, 669, 851, 1010, 1316
HhaI	GCGC	1 cut(s)	1412
Hin1I	GRCGYC	1 cut(s)	66
Hin1II	CATG	7 cut(s)	295, 758, 764, 886, 1282, 1289, 1397
Hin6I	GCGC	1 cut(s)	1410
HinP1I	GCGC	1 cut(s)	1410
HindIII	AAGCTT	3 cut(s)	97, 455, 680
HinfI	GANTC	3 cut(s)	56, 107, 697
HphI	GGTGA	2 cut(s)	548, 631
Hpy166II	GTNNAC	3 cut(s)	199, 631, 989
Hpy188I	TCNGA	8 cut(s)	216, 235, 577, 727, 827, 1173, 1330, 1377
Hpy188III	TCNNGA	2 cut(s)	1207, 1243
Hpy8I	GTNNAC	3 cut(s)	199, 631, 989
Hpy99I	CGWCG	2 cut(s)	50, 71
HpyAV	CCTTC	2 cut(s)	384, 944
HpyCH4III	ACNGT	3 cut(s)	788, 875, 1234
HpyCH4IV	ACGT	4 cut(s)	66, 158, 362, 1120
HpyF3I	CTNAG	3 cut(s)	215, 1053, 1137
HpySE526I	ACGT	4 cut(s)	66, 158, 362, 1120
Hsp92I	GRCGYC	1 cut(s)	66
Hsp92II	CATG	7 cut(s)	295, 758, 764, 886, 1282, 1289, 1397
HspAI	GCGC	1 cut(s)	1410
Kzo9I	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
LmnI	GCTCC	3 cut(s)	47, 827, 970
Lsp1109I	GCAGC	2 cut(s)	382, 487
LweI	GCATC	3 cut(s)	319, 421, 665
MaeI	CTAG	2 cut(s)	408, 489
MaeII	ACGT	4 cut(s)	66, 158, 362, 1120
MaeIII	GTNAC	3 cut(s)	807, 1078, 1185
MalI	GATC	8 cut(s)	213, 321, 471, 579, 822, 1156, 1335, 1374
MboI	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
MboII	GAAGA	3 cut(s)	6, 28, 1343
MfeI	CAATTG	1 cut(s)	182
MflI	RGATCY	4 cut(s)	211, 820, 1154, 1333
MhlI	GDGCHC	1 cut(s)	221
MluCI	AATT	6 cut(s)	85, 176, 182, 996, 1047, 1090
MlyI	GAGTC	1 cut(s)	706
MseI	TTAA	6 cut(s)	74, 366, 903, 999, 1089, 1401
MslI	CAYNNNNRTG	4 cut(s)	296, 759, 839, 876
MspR9I	CCNGG	4 cut(s)	81, 113, 673, 1116
MunI	CAATTG	1 cut(s)	182
MvaI	CCWGG	4 cut(s)	81, 113, 673, 1116
MvnI	CGCG	1 cut(s)	1075
NdeII	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
NlaIII	CATG	7 cut(s)	295, 758, 764, 886, 1282, 1289, 1397
NlaIV	GGNNCC	1 cut(s)	670
NmeAIII	GCCGAG	1 cut(s)	827
NmuCI	GTSAC	3 cut(s)	807, 1078, 1185
PaqCI	CACCTGC	1 cut(s)	878
PcsI	WCGNNNNNNNCGW	1 cut(s)	155
PfeI	GAWTC	2 cut(s)	56, 107
PflFI	GACNNNGTC	1 cut(s)	160
PflMI	CCANNNNNTGG	1 cut(s)	608
PfoI	TCCNGGA	1 cut(s)	1114
PkrI	GCNGC	5 cut(s)	7, 52, 372, 502, 511
PleI	GAGTC	1 cut(s)	705
PpsI	GAGTC	1 cut(s)	705
PsiI	TTATAA	1 cut(s)	1262
Psp6I	CCWGG	4 cut(s)	79, 111, 671, 1114
PspFI	CCCAGC	1 cut(s)	1097
PspGI	CCWGG	4 cut(s)	79, 111, 671, 1114
PspN4I	GGNNCC	1 cut(s)	670
PspPI	GGNCC	3 cut(s)	92, 668, 1390
PstI	CTGCAG	1 cut(s)	1306
PstNI	CAGNNNCTG	2 cut(s)	817, 1157
PsuI	RGATCY	4 cut(s)	211, 820, 1154, 1333
PsyI	GACNNNGTC	1 cut(s)	160
RseI	CAYNNNNRTG	4 cut(s)	296, 759, 839, 876
SaqAI	TTAA	6 cut(s)	74, 366, 903, 999, 1089, 1401
SatI	GCNGC	5 cut(s)	6, 51, 371, 501, 510
Sau3AI	GATC	8 cut(s)	211, 319, 469, 577, 820, 1154, 1333, 1372
Sau96I	GGNCC	3 cut(s)	92, 668, 1390
SchI	GAGTC	1 cut(s)	706
ScrFI	CCNGG	4 cut(s)	81, 113, 673, 1116
SduI	GDGCHC	1 cut(s)	221
SfaNI	GCATC	3 cut(s)	319, 421, 665
SfcI	CTRYAG	2 cut(s)	1302, 1415
SinI	GGWCC	1 cut(s)	1390
SmiMI	CAYNNNNRTG	4 cut(s)	296, 759, 839, 876
SpeI	ACTAGT	1 cut(s)	488
Sse9I	AATT	6 cut(s)	85, 176, 182, 996, 1047, 1090
SsiI	CCGC	4 cut(s)	6, 51, 509, 1073
SspMI	CTAG	2 cut(s)	408, 489
StyD4I	CCNGG	4 cut(s)	79, 111, 671, 1114
StyI	CCWWGG	1 cut(s)	435
TaaI	ACNGT	3 cut(s)	788, 875, 1234
TaiI	ACGT	4 cut(s)	69, 161, 365, 1123
TaqI	TCGA	4 cut(s)	472, 712, 780, 1206
TasI	AATT	6 cut(s)	85, 176, 182, 996, 1047, 1090
TauI	GCSGC	3 cut(s)	8, 53, 512
TfiI	GAWTC	2 cut(s)	56, 107
Tru1I	TTAA	6 cut(s)	74, 366, 903, 999, 1089, 1401
Tru9I	TTAA	6 cut(s)	74, 366, 903, 999, 1089, 1401
TscAI	CASTG	3 cut(s)	822, 880, 1311
TseFI	GTSAC	3 cut(s)	807, 1078, 1185
TseI	GCWGC	2 cut(s)	370, 500
Tsp45I	GTSAC	3 cut(s)	807, 1078, 1185
TspDTI	ATGAA	5 cut(s)	273, 314, 350, 1170, 1267
TspGWI	ACGGA	2 cut(s)	34, 312
TspRI	CASTG	3 cut(s)	822, 880, 1311
Tth111I	GACNNNGTC	1 cut(s)	160
Van91I	CCANNNNNTGG	1 cut(s)	608
VpaK11BI	GGWCC	1 cut(s)	1390
XapI	RAATTY	2 cut(s)	85, 176
XcmI	CCANNNNNNNNNTGG	1 cut(s)	975
XspI	CTAG	2 cut(s)	408, 489
ZraI	GACGTC	1 cut(s)	67

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.