Rorug02G0256600

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003115
Physical Location & Seq
Forward (+)
26876197 .. 26879215
3019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0256600.1

Sequence Viewer

Length: 2391 bp
ATGAGAAACTCTGCACTTCACCTAAGACATTATCACAAGTCCATCAGTTCCAAGCAGCAATTCCTCAACTTCTCGTCATTACCATCCCCTGTTCTCGAATCACAAAACCCATGTCTTCGAATCCAAACCCTCCAAACCCACAGGCTTGCATTAGTCCATCTCTTGCAACTAGGCGTTTCAGACAAACCCATTTTGTTCTTCAAGAGAATCCATGCTCAGATTCTCGCATCGGGCTTTCAAGAAGATGTTTTCCTTGCCAATCTTCTACTGGACTCGTACAAGAAATCTGGGTCTATTGTTTATGCACGGAAGCTGTTTGATAGAATGCCTGAGAGGAACTCCGTTACTTGGTCTTCGATGGTTTCTATGTATACCAAGCATGGGAAGAGCGAAGAAGCGCTGAAGGTGTTTTCGGAGTTCCATAGGAGTTCTGATGGGAGGCCGAATGAGTATACCTTGCCTAGTGTTATTCGGGCTTGTACGCAATTTGGAGGGGTTGATCAAGGTGCCCAAGTGCATTGTTTTGTTGTTAAGACTGGTTTTGATCTGGAAGTCTTTGTGGGTACTTCATTGATTGATTTTTATGGAAAAATGGGGGATATAGAAGAGGCGAGATTGATTTTTGATGGTTTAAAAGTTAAAAGTGCGGTTACTTGGACTATAGTTATAGCAGGGTATGCGAAAAGTGGAAGAAGTGAAGCTGCTTTGAAGTTGTTTTATCATATGAAAGACACTGGTGTTCTTCCTGATAAATATGTGCTCTCTGCTCTTCTGACCGCATGTTCTGGCATCAAGTTCATTGGAGGCGGCAAGCAAATTCATGCTCATGTGCTGAGAAGGGGAACAGAAATGGATGTTTCGGTGGTTAATGTACTAATAGATTTCTACACAAAGTGCGGTCAAGTCCAGGCAGGACATAAACTATTTGATAAGATGGTAGATAAGGACCTTATTTCATGGACCACAATGATAGCAGGGTACATGCAAAACTCGATGCACATGGAGGCTGTTAAGCTATTTTCTGAAATGACCAGATTGGGTTGGAAGCCAGACGGATATGGGTGTAGTAGCATTCTTACTTCATGTGGTTCACTTGAGGCTCTGAAACATGGGAGAGAAGTGCACGCTTATACTATCAGGGTTGATCTTGTCTATGAATATTATGTGAAGAATAGTTTGATTGATATGTATGCAAAATGTGATTCCCTGACTGATGCAAGAAGAGTTTTTGATTCCATGACTGATCATAATGTGGTCTCTTACAATGCAATGATTGAAGGATACTCAAGGCAGGATAAGGTGGCTGAAGCTCTAGATCTTTTCAATCTTATGAGGCTTAAAATGGTCCAGCCTAGTATCTTGACATTTGTCAGTCTTCTTGGTGTCTCAGCTGCTTTATTGACCTTGGAATTAAGCAAGCAAATCCATGGGCTGACCACCAAGTACGGGTTATCTTTGGATATTTTTGCTGGCAGTGCTCTCATTGATGTTTATTCCAAGTGTTCATGTATTCGCGAAGCTAAACTTGTATTCGAGGAGATGAATGAAAAAGACATAGTAGTGTGGAATTCAATGTTCTGCGGATACGCCCAACAGCTGGAAAGTGAAGAGGCTCTTAAGTTGTACTCAGAACTACAATTATCAAGACAAAATCCTAATGAGTTTACTTTTGCTTCCCTAATCTCAGCAGCCAGCAACCTAGCAAGTATCCAACATGGTCAACAGTTCCATAGCCAGATCTTTAAAATTGGTCTTGAGAATGATCCTTTTGTCACAAATGCCCTTGTGGATATGTATTCCAAGTGTGGAAGCATTAAAGAGGCTCGCAAAATATTTGATTCCAAAACTTTGAAAGATGTTGCCTGTTGGAATTCCATTATCTCAACGTATGCTCATCATGGAGAAGCAGAAAAAGCTCTTCTGATGTTTGAAAAAATGATGAATGAGGGGATAAAACCCAACTATATCACCTTTGTGGGGGTGCTATCAGCTTGTAGCCATGCAGGCCTTGTGGAAGATGGACTTGGTCACTTTGAATCAATGCCTTGGTTTGGAATCGAACCAGGGATAGAACATTATTCTTGCATTGTTTCTCTATTGGGCCGCGCCGGTAAATTATCTGAAGCTAAGGAATTTATTGAGAAAATGCCAATGAAACCACCAGCAATTATATGGAGGAGTTTACTCAGTTCATGTACAGCTGCAGGTAATGTTGAATTGGGGATCTACGCAGCAGAGATGGCAATTTTGAGTGATCCAATGGATAGTGGATCGTATATCTTACTTTCAAATATGTATGCATCCGAAGGTATGTGGGATGATGTCAAGAGGGTGAGGAAAAAAATGGACTATAATGGTGTAGTGAAAGAAACCGGACGTAGTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

796

Amino Acids

89.12

Weight (kDa)

7.11

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 112 - 160 1.2e-06 PPR repeat family
PPR PF01535 115 - 142 2.1e-06 PPR repeat
PPR_2 PF13041 215 - 261 5.3e-11 PPR repeat family
PPR PF01535 217 - 246 6.8e-07 PPR repeat
PPR_2 PF13041 316 - 362 2.2e-09 PPR repeat family
PPR PF01535 318 - 347 9e-07 PPR repeat
PPR_2 PF13041 417 - 460 3.7e-11 PPR repeat family
PPR_3 PF13812 417 - 462 8.1e-06 Pentatricopeptide repeat domain
PPR PF01535 419 - 446 1.5e-06 PPR repeat
PPR_2 PF13041 517 - 563 3.3e-09 PPR repeat family
PPR_2 PF13041 618 - 666 3.1e-10 PPR repeat family
PPR PF01535 622 - 651 5.9e-06 PPR repeat
E_motif PF20431 737 - 795 4.4e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2195
AccB1I GGYRCC 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 1597
AccI GTMKAC 2 cut(s) 371, 452
AccII CGCG 2 cut(s) 1515, 2106
AciI CCGC 6 cut(s) 647, 777, 807, 897, 1581, 2104
AclWI GGATC 4 cut(s) 1757, 2231, 2249, 2278
AcsI RAATTY 4 cut(s) 816, 1567, 1870, 2132
AcuI CTGAAG 3 cut(s) 422, 1326, 2142
AdeI CACNNNGTG 1 cut(s) 894
AfaI GTAC 8 cut(s) 278, 481, 565, 873, 980, 1445, 1625, 2197
AfeI AGCGCT 1 cut(s) 399
AfiI CCNNNNNNNGG 6 cut(s) 348, 381, 1446, 1597, 1977, 2051
AflII CTTAAG 1 cut(s) 1616
AjnI CCWGG 2 cut(s) 906, 2062
AjuI GAANNNNNNNTTGG 4 cut(s) 44, 76, 2007, 2039
AleI CACNNNNGTG 1 cut(s) 1973
Alw21I GWGCWC 3 cut(s) 762, 1125, 1480
Alw26I GTCTC 2 cut(s) 1261, 1390
Alw44I GTGCAC 1 cut(s) 1121
AlwI GGATC 4 cut(s) 1757, 2231, 2249, 2278
Aor51HI AGCGCT 1 cut(s) 399
AoxI GGCC 3 cut(s) 440, 2005, 2101
ApaLI GTGCAC 1 cut(s) 1121
ApeKI GCWGC 6 cut(s) 55, 701, 1391, 1688, 2201, 2231
ApoI RAATTY 4 cut(s) 816, 1567, 1870, 2132
ArsI GACNNNNNNTTYG 2 cut(s) 97, 129
AspLEI GCGC 2 cut(s) 400, 2108
AspS9I GGNCC 4 cut(s) 946, 960, 1345, 2101
AsuHPI GGTGA 3 cut(s) 11, 1960, 2344
AsuII TTCGAA 1 cut(s) 118
AvaII GGWCC 3 cut(s) 946, 960, 1345
BaeGI GKGCMC 2 cut(s) 511, 1125
BanI GGYRCC 1 cut(s) 506
BbsI GAAGAC 3 cut(s) 107, 345, 1367
Bbv12I GWGCWC 3 cut(s) 762, 1125, 1480
BbvI GCAGC 6 cut(s) 67, 688, 1378, 1700, 2188, 2243
BccI CCATC 9 cut(s) 50, 91, 165, 352, 428, 620, 928, 2012, 2233
BciT130I CCWGG 2 cut(s) 908, 2064
BciVI GTATCC 3 cut(s) 1274, 1577, 1718
BclI TGATCA 2 cut(s) 499, 1243
BcoDI GTCTC 2 cut(s) 1261, 1390
BfaI CTAG 5 cut(s) 170, 462, 1313, 1353, 1700
BfmI CTRYAG 2 cut(s) 660, 2202
BfoI RGCGCY 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 1616
BfuAI ACCTGC 1 cut(s) 2195
BfuI GTATCC 3 cut(s) 1274, 1577, 1718
BglI GCCNNNNNGGC 1 cut(s) 2004
BglII AGATCT 2 cut(s) 1315, 1737
BisI GCNGC 8 cut(s) 56, 702, 808, 1392, 1689, 2104, 2202, 2232
BlsI GCNGC 8 cut(s) 57, 703, 809, 1393, 1690, 2105, 2203, 2233
Bme1390I CCNGG 2 cut(s) 908, 2064
Bme18I GGWCC 3 cut(s) 946, 960, 1345
BmgT120I GGNCC 4 cut(s) 946, 960, 1345, 2101
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 2 cut(s) 908, 2064
BmsI GCATC 5 cut(s) 236, 798, 984, 1204, 2309
BoxI GACNNNNGTC 1 cut(s) 1367
BpiI GAAGAC 3 cut(s) 107, 345, 1367
BplI GAGNNNNNCTC 2 cut(s) 323, 355
Bpu10I CCTNAGC 1 cut(s) 2127
Bpu14I TTCGAA 1 cut(s) 118
BpuEI CTTGAG 3 cut(s) 1115, 1270, 1775
BsaI GGTCTC 1 cut(s) 1261
BsaJI CCNNGG 4 cut(s) 1404, 1426, 2045, 2063
BsaWI WCCGGW 1 cut(s) 2372
Bsc4I CCNNNNNNNGG 6 cut(s) 348, 381, 1446, 1597, 1977, 2051
Bse118I RCCGGY 1 cut(s) 2108
Bse1I ACTGG 3 cut(s) 273, 541, 739
Bse3DI GCAATG 1 cut(s) 1275
BseBI CCWGG 2 cut(s) 908, 2064
BseDI CCNNGG 4 cut(s) 1404, 1426, 2045, 2063
BseGI GGATG 4 cut(s) 83, 859, 2300, 2323
BseLI CCNNNNNNNGG 6 cut(s) 348, 381, 1446, 1597, 1977, 2051
BseMI GCAATG 1 cut(s) 1275
BseMII CTCAG 7 cut(s) 230, 321, 824, 1401, 1641, 1698, 2200
BseNI ACTGG 3 cut(s) 273, 541, 739
BseRI GAGGAG 2 cut(s) 1550, 2191
BseSI GKGCMC 2 cut(s) 511, 1125
BseXI GCAGC 6 cut(s) 67, 688, 1378, 1700, 2188, 2243
Bsh1236I CGCG 2 cut(s) 1515, 2106
BshFI GGCC 3 cut(s) 442, 2007, 2103
BshNI GGYRCC 1 cut(s) 506
BsiHKAI GWGCWC 3 cut(s) 762, 1125, 1480
BsiSI CCGG 2 cut(s) 2109, 2373
BslI CCNNNNNNNGG 6 cut(s) 348, 381, 1446, 1597, 1977, 2051
BsmAI GTCTC 2 cut(s) 1261, 1390
BsmI GAATGC 2 cut(s) 330, 1071
BsnI GGCC 3 cut(s) 442, 2007, 2103
Bso31I GGTCTC 1 cut(s) 1261
Bsp119I TTCGAA 1 cut(s) 118
Bsp1286I GDGCHC 4 cut(s) 511, 762, 1125, 1480
Bsp1407I TGTACA 1 cut(s) 2195
Bsp19I CCATGG 1 cut(s) 1426
Bsp68I TCGCGA 1 cut(s) 1515
BspACI CCGC 6 cut(s) 647, 777, 807, 897, 1581, 2104
BspANI GGCC 3 cut(s) 442, 2007, 2103
BspCNI CTCAG 7 cut(s) 229, 322, 825, 1400, 1640, 1697, 2199
BspFNI CGCG 2 cut(s) 1515, 2106
BspLI GGNNCC 1 cut(s) 508
BspMAI CTGCAG 1 cut(s) 2206
BspMI ACCTGC 1 cut(s) 2195
BspPI GGATC 4 cut(s) 1757, 2231, 2249, 2278
BspQI GCTCTTC 3 cut(s) 380, 774, 1923
BspT104I TTCGAA 1 cut(s) 118
BspT107I GGYRCC 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 1616
BspTNI GGTCTC 1 cut(s) 1261
BsrDI GCAATG 1 cut(s) 1275
BsrFI RCCGGY 1 cut(s) 2108
BsrGI TGTACA 1 cut(s) 2195
BsrI ACTGG 3 cut(s) 273, 541, 739
BssAI RCCGGY 1 cut(s) 2108
BssECI CCNNGG 4 cut(s) 1404, 1426, 2045, 2063
BssNAI GTATAC 2 cut(s) 372, 453
BssT1I CCWWGG 3 cut(s) 1404, 1426, 2045
Bst1107I GTATAC 2 cut(s) 372, 453
Bst2UI CCWGG 2 cut(s) 908, 2064
Bst4CI ACNGT 1 cut(s) 1725
Bst6I CTCTTC 6 cut(s) 380, 600, 774, 1216, 1602, 1923
BstAFI CTTAAG 1 cut(s) 1616
BstAPI GCANNNNNTGC 1 cut(s) 677
BstAUI TGTACA 1 cut(s) 2195
BstBI TTCGAA 1 cut(s) 118
BstC8I GCNNGC 8 cut(s) 147, 812, 1125, 1418, 1471, 1693, 1825, 2005
BstDEI CTNAG 9 cut(s) 23, 216, 330, 833, 1387, 1627, 1684, 2127, 2186
BstDSI CCRYGG 1 cut(s) 1426
BstF5I GGATG 4 cut(s) 83, 859, 2300, 2323
BstFNI CGCG 2 cut(s) 1515, 2106
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 400, 2108
BstMAI GTCTC 2 cut(s) 1261, 1390
BstMWI GCNNNNNNNGC 5 cut(s) 677, 1475, 1913, 2004, 2240
BstNI CCWGG 2 cut(s) 908, 2064
BstNSI RCATGY 2 cut(s) 783, 985
BstPAI GACNNNNGTC 1 cut(s) 1367
BstSCI CCNGG 2 cut(s) 906, 2062
BstSFI CTRYAG 2 cut(s) 660, 2202
BstSLI GKGCMC 2 cut(s) 511, 1125
BstUI CGCG 2 cut(s) 1515, 2106
BstV1I GCAGC 6 cut(s) 67, 688, 1378, 1700, 2188, 2243
BstV2I GAAGAC 3 cut(s) 107, 345, 1367
BstX2I RGATCY 3 cut(s) 1315, 1737, 2223
BstYI RGATCY 3 cut(s) 1315, 1737, 2223
BstZ17I GTATAC 2 cut(s) 372, 453
BsuI GTATCC 3 cut(s) 1274, 1577, 1718
BsuRI GGCC 3 cut(s) 442, 2007, 2103
BtgI CCRYGG 1 cut(s) 1426
BtsCI GGATG 4 cut(s) 83, 859, 2300, 2323
BtsI GCAGTG 1 cut(s) 1480
BtsIMutI CAGTG 2 cut(s) 732, 1480
BtuMI TCGCGA 1 cut(s) 1515
BveI ACCTGC 1 cut(s) 2195
Cac8I GCNNGC 8 cut(s) 147, 812, 1125, 1418, 1471, 1693, 1825, 2005
CfoI GCGC 2 cut(s) 400, 2108
Cfr10I RCCGGY 1 cut(s) 2108
Cfr13I GGNCC 4 cut(s) 946, 960, 1345, 2101
Csp6I GTAC 8 cut(s) 277, 480, 564, 872, 979, 1444, 1624, 2196
CviQI GTAC 8 cut(s) 277, 480, 564, 872, 979, 1444, 1624, 2196
DdeI CTNAG 9 cut(s) 23, 216, 330, 833, 1387, 1627, 1684, 2127, 2186
DraI TTTAAA 2 cut(s) 633, 1744
DraIII CACNNNGTG 1 cut(s) 894
Eam1104I CTCTTC 6 cut(s) 380, 600, 774, 1216, 1602, 1923
EarI CTCTTC 6 cut(s) 380, 600, 774, 1216, 1602, 1923
Eco130I CCWWGG 3 cut(s) 1404, 1426, 2045
Eco147I AGGCCT 1 cut(s) 2007
Eco31I GGTCTC 1 cut(s) 1261
Eco47I GGWCC 3 cut(s) 946, 960, 1345
Eco47III AGCGCT 1 cut(s) 399
Eco57I CTGAAG 3 cut(s) 422, 1326, 2142
EcoO109I RGGNCCY 1 cut(s) 946
EcoRI GAATTC 2 cut(s) 1567, 1870
EcoRII CCWGG 2 cut(s) 906, 2062
EcoT14I CCWWGG 3 cut(s) 1404, 1426, 2045
EcoT22I ATGCAT 1 cut(s) 2302
ErhI CCWWGG 3 cut(s) 1404, 1426, 2045
FalI AAGNNNNNCTT 6 cut(s) 1509, 1541, 1599, 1631, 2007, 2039
FauNDI CATATG 1 cut(s) 723
FbaI TGATCA 2 cut(s) 499, 1243
FblI GTMKAC 2 cut(s) 371, 452
Fnu4HI GCNGC 8 cut(s) 56, 702, 808, 1392, 1689, 2104, 2202, 2232
FokI GGATG 4 cut(s) 70, 866, 2287, 2330
Fsp4HI GCNGC 8 cut(s) 56, 702, 808, 1392, 1689, 2104, 2202, 2232
FspBI CTAG 5 cut(s) 170, 462, 1313, 1353, 1700
GlaI GCGC 2 cut(s) 399, 2107
GluI GCNGC 8 cut(s) 56, 702, 808, 1392, 1689, 2104, 2202, 2232
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 3 cut(s) 442, 2007, 2103
HapII CCGG 2 cut(s) 2109, 2373
HhaI GCGC 2 cut(s) 400, 2108
Hin6I GCGC 2 cut(s) 398, 2106
HinP1I GCGC 2 cut(s) 398, 2106
HincII GTYRAC 1 cut(s) 1721
HindII GTYRAC 1 cut(s) 1721
HpaII CCGG 2 cut(s) 2109, 2373
HphI GGTGA 3 cut(s) 11, 1960, 2344
Hpy166II GTNNAC 7 cut(s) 372, 453, 1091, 1123, 1665, 1721, 2183
Hpy8I GTNNAC 7 cut(s) 372, 453, 1091, 1123, 1665, 1721, 2183
HpyAV CCTTC 4 cut(s) 397, 831, 1271, 2300
HpyCH4III ACNGT 1 cut(s) 1725
HpyCH4IV ACGT 2 cut(s) 1886, 2377
HpyF10VI GCNNNNNNNGC 5 cut(s) 677, 1475, 1913, 2004, 2240
HpyF3I CTNAG 9 cut(s) 23, 216, 330, 833, 1387, 1627, 1684, 2127, 2186
HpySE526I ACGT 2 cut(s) 1886, 2377
HspAI GCGC 2 cut(s) 398, 2106
Ksp22I TGATCA 2 cut(s) 499, 1243
LguI GCTCTTC 3 cut(s) 380, 774, 1923
Lsp1109I GCAGC 6 cut(s) 67, 688, 1378, 1700, 2188, 2243
LweI GCATC 5 cut(s) 236, 798, 984, 1204, 2309
MaeI CTAG 5 cut(s) 170, 462, 1313, 1353, 1700
MaeII ACGT 2 cut(s) 1886, 2377
MaeIII GTNAC 4 cut(s) 343, 649, 1771, 2027
MflI RGATCY 3 cut(s) 1315, 1737, 2223
MhlI GDGCHC 4 cut(s) 511, 762, 1125, 1480
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 3 cut(s) 1022, 1735, 1847
Mph1103I ATGCAT 1 cut(s) 2302
MslI CAYNNNNRTG 3 cut(s) 825, 1559, 1973
MspA1I CMGCKG 3 cut(s) 1391, 1597, 2201
MspCI CTTAAG 1 cut(s) 1616
MspI CCGG 2 cut(s) 2109, 2373
MspR9I CCNGG 2 cut(s) 908, 2064
Mva1269I GAATGC 2 cut(s) 330, 1071
MvaI CCWGG 2 cut(s) 908, 2064
MvnI CGCG 2 cut(s) 1515, 2106
MwoI GCNNNNNNNGC 5 cut(s) 677, 1475, 1913, 2004, 2240
NcoI CCATGG 1 cut(s) 1426
NdeI CATATG 1 cut(s) 723
NlaIV GGNNCC 1 cut(s) 508
NmuCI GTSAC 2 cut(s) 1771, 2027
NruI TCGCGA 1 cut(s) 1515
NsiI ATGCAT 1 cut(s) 2302
NspI RCATGY 2 cut(s) 783, 985
NspV TTCGAA 1 cut(s) 118
OliI CACNNNNGTG 1 cut(s) 1973
PceI AGGCCT 1 cut(s) 2007
PciSI GCTCTTC 3 cut(s) 380, 774, 1923
PctI GAATGC 2 cut(s) 330, 1071
PfeI GAWTC 9 cut(s) 98, 120, 207, 220, 1202, 1232, 1838, 2036, 2055
PflFI GACNNNGTC 1 cut(s) 2025
PflMI CCANNNNNTGG 1 cut(s) 1597
PkrI GCNGC 8 cut(s) 57, 703, 809, 1393, 1690, 2105, 2203, 2233
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 946
PshAI GACNNNNGTC 1 cut(s) 1367
Psp5II RGGWCCY 1 cut(s) 946
Psp6I CCWGG 2 cut(s) 906, 2062
PspGI CCWGG 2 cut(s) 906, 2062
PspN4I GGNNCC 1 cut(s) 508
PspPI GGNCC 4 cut(s) 946, 960, 1345, 2101
PspPPI RGGWCCY 1 cut(s) 946
PstI CTGCAG 1 cut(s) 2206
PsuI RGATCY 3 cut(s) 1315, 1737, 2223
PsyI GACNNNGTC 1 cut(s) 2025
PvuII CAGCTG 3 cut(s) 1391, 1597, 2201
RruI TCGCGA 1 cut(s) 1515
RsaI GTAC 8 cut(s) 278, 481, 565, 873, 980, 1445, 1625, 2197
RsaNI GTAC 8 cut(s) 277, 480, 564, 872, 979, 1444, 1624, 2196
RseI CAYNNNNRTG 3 cut(s) 825, 1559, 1973
SapI GCTCTTC 3 cut(s) 380, 774, 1923
SatI GCNGC 8 cut(s) 56, 702, 808, 1392, 1689, 2104, 2202, 2232
Sau96I GGNCC 4 cut(s) 946, 960, 1345, 2101
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 2 cut(s) 908, 2064
SduI GDGCHC 4 cut(s) 511, 762, 1125, 1480
SfaNI GCATC 5 cut(s) 236, 798, 984, 1204, 2309
SfcI CTRYAG 2 cut(s) 660, 2202
SfuI TTCGAA 1 cut(s) 118
SinI GGWCC 3 cut(s) 946, 960, 1345
SmiMI CAYNNNNRTG 3 cut(s) 825, 1559, 1973
SmlI CTYRAG 4 cut(s) 1094, 1285, 1616, 1754
SmoI CTYRAG 4 cut(s) 1094, 1285, 1616, 1754
SseBI AGGCCT 1 cut(s) 2007
SsiI CCGC 6 cut(s) 647, 777, 807, 897, 1581, 2104
SspI AATATT 2 cut(s) 1160, 1833
SspMI CTAG 5 cut(s) 170, 462, 1313, 1353, 1700
StuI AGGCCT 1 cut(s) 2007
StyD4I CCNGG 2 cut(s) 906, 2062
StyI CCWWGG 3 cut(s) 1404, 1426, 2045
TaaI ACNGT 1 cut(s) 1725
TaiI ACGT 2 cut(s) 1889, 2380
TaqI TCGA 6 cut(s) 96, 118, 356, 992, 1533, 2058
TatI WGTACW 3 cut(s) 871, 1623, 2195
TauI GCSGC 2 cut(s) 810, 2106
TfiI GAWTC 9 cut(s) 98, 120, 207, 220, 1202, 1232, 1838, 2036, 2055
TscAI CASTG 2 cut(s) 739, 1480
TseFI GTSAC 2 cut(s) 1771, 2027
TseI GCWGC 6 cut(s) 55, 701, 1391, 1688, 2201, 2231
Tsp45I GTSAC 2 cut(s) 1771, 2027
TspGWI ACGGA 3 cut(s) 322, 331, 1068
TspRI CASTG 2 cut(s) 739, 1480
Tth111I GACNNNGTC 1 cut(s) 2025
Van91I CCANNNNNTGG 1 cut(s) 1597
Vha464I CTTAAG 1 cut(s) 1616
VneI GTGCAC 1 cut(s) 1121
VpaK11BI GGWCC 3 cut(s) 946, 960, 1345
XapI RAATTY 4 cut(s) 816, 1567, 1870, 2132
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 2 cut(s) 783, 985
XcmI CCANNNNNNNNNTGG 2 cut(s) 265, 2169
XmiI GTMKAC 2 cut(s) 371, 452
XspI CTAG 5 cut(s) 170, 462, 1313, 1353, 1700
Zsp2I ATGCAT 1 cut(s) 2302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.