FvH4_3g34760

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003115
Physical Location & Seq
Reverse (-)
30079143 .. 30080042
900 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34760.t1

Sequence Viewer

Length: 900 bp
ATGGCAATCCAAAACTCAATTCCTCTTCCCCTCAAGGCCATAACATGCCTCTTCCTTCTAGCTCTTACAATAACACACACCACCTCAGCCAGAGTCCTTGATGAAGAAACCGACACAACTCAAACCCAAATACCTGTTTCCACCGTACCTCCTCCTGTCGTCGGTGCCACAACAGCCGGCCCGACCGCTACGGTGGCCAACGGGGAGTCCTACCATCCGCTTACATTCTTTCTGCATGATATAATCGGTGGGACTAACCCTTCAGCCAGAGCCGTGACTGGCATTGTGACCAACCCTGCTGTCAATGGCCAAGTCCCCTTTGCTAAACCTAATGGAGCAGTCATCGGCGGGAACAATGGCATTCCCCAAAACAACAACAACAATGGAATCATCAACAATAACAACGTCCCCTTCTTCACCGGCCTCGGAGGAAACATCAACAACAACCTCAGACAAAGAAACAACAACAACAACTTCATCGGCGGGAACAATGGGTTCCCCGTCGTGAACGGCGCCCAACTCCCACCAGGACAGTCCCTTCAGAAGCTCATGTTCGGCACACTCACGGTCTTTGACGATGAGCTCACTGAAGGCCACGAGCTTGGCTCTGGCTTGTTGGGCAAGGCACAAGGATTTTACGTCGCTAGTTCTGAAGATGGGAGCAGCCAGACCATTGCTTTCACTGCAATGTTTGAGAGTGGTGGGTATGCTGATAGCCTCACATTCTTTGGGGTTCACACAATGGCTTCGTCTGAGTCTCATCTTGCAGTTATGGGAGGGACCGGAAAGTACTTGAACGCAAAAGGGTATGCTTTGGTTAAGACCATCAATGGAGTGAATGAGCAAAATACTGATGGAGTTGACACCGTTCTTGAATTTACTGTGTATCTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

300

Amino Acids

31.25

Weight (kDa)

4.97

Isoelectric Point (pI)

26.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 165 - 297 9.4e-29 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 164, 512
AciI CCGC 4 cut(s) 186, 218, 348, 483
AcoI YGGCCR 2 cut(s) 195, 307
AcsI RAATTY 1 cut(s) 875
AcuI CTGAAG 4 cut(s) 246, 524, 609, 672
AcyI GRCGYC 1 cut(s) 513
AfaI GTAC 2 cut(s) 147, 791
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 2 cut(s) 796, 875
AjnI CCWGG 1 cut(s) 526
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AluBI AGCT 4 cut(s) 62, 547, 583, 601
AluI AGCT 4 cut(s) 62, 547, 583, 601
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 1 cut(s) 762
AoxI GGCC 6 cut(s) 36, 178, 195, 307, 421, 592
ApeKI GCWGC 1 cut(s) 663
ApoI RAATTY 1 cut(s) 875
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 2 cut(s) 179, 780
AsuHPI GGTGA 1 cut(s) 409
AvaII GGWCC 1 cut(s) 780
BalI TGGCCA 2 cut(s) 197, 309
BanI GGYRCC 2 cut(s) 164, 512
BanII GRGCYC 1 cut(s) 585
BauI CACGAG 1 cut(s) 596
Bbv12I GWGCWC 1 cut(s) 585
BbvCI CCTCAGC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 675
BccI CCATC 4 cut(s) 222, 650, 833, 848
BceAI ACGGC 2 cut(s) 257, 526
BciT130I CCWGG 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 762
BfaI CTAG 3 cut(s) 59, 645, 898
BfoI RGCGCY 1 cut(s) 516
BisI GCNGC 1 cut(s) 664
BlsI GCNGC 1 cut(s) 665
BmcAI AGTACT 1 cut(s) 791
Bme1390I CCNGG 1 cut(s) 528
Bme18I GGWCC 1 cut(s) 780
BmgT120I GGNCC 2 cut(s) 179, 780
BmiI GGNNCC 4 cut(s) 166, 497, 514, 781
BmrFI CCNGG 1 cut(s) 528
BplI GAGNNNNNCTC 2 cut(s) 590, 622
Bpu10I CCTNAGC 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 17
BsaHI GRCGYC 1 cut(s) 513
BsaJI CCNNGG 1 cut(s) 424
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse118I RCCGGY 2 cut(s) 176, 419
Bse1I ACTGG 1 cut(s) 283
Bse3DI GCAATG 2 cut(s) 672, 693
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMI GCAATG 2 cut(s) 672, 693
BseMII CTCAG 3 cut(s) 99, 463, 744
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 675
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 6 cut(s) 38, 180, 197, 309, 423, 594
BshNI GGYRCC 2 cut(s) 164, 512
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 585
BsiSI CCGG 3 cut(s) 177, 420, 783
BslFI GGGAC 5 cut(s) 265, 299, 392, 520, 793
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 762
BsmFI GGGAC 5 cut(s) 265, 299, 392, 520, 793
BsmI GAATGC 1 cut(s) 360
BsnI GGCC 6 cut(s) 38, 180, 197, 309, 423, 594
Bsp1286I GDGCHC 1 cut(s) 585
BspACI CCGC 4 cut(s) 186, 218, 348, 483
BspANI GGCC 6 cut(s) 38, 180, 197, 309, 423, 594
BspCNI CTCAG 3 cut(s) 98, 462, 745
BspLI GGNNCC 4 cut(s) 166, 497, 514, 781
BspT107I GGYRCC 2 cut(s) 164, 512
BsrDI GCAATG 2 cut(s) 672, 693
BsrFI RCCGGY 2 cut(s) 176, 419
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 2 cut(s) 176, 419
BssECI CCNNGG 1 cut(s) 424
BssNI GRCGYC 1 cut(s) 513
BssSI CACGAG 1 cut(s) 596
Bst2BI CACGAG 1 cut(s) 596
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 6 cut(s) 145, 193, 534, 568, 868, 883
Bst6I CTCTTC 2 cut(s) 30, 56
BstACI GRCGYC 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 4 cut(s) 85, 449, 753, 890
BstF5I GGATG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstMAI GTCTC 1 cut(s) 762
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 4 cut(s) 173, 194, 618, 683
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 526
BstV1I GCAGC 1 cut(s) 675
BstXI CCANNNNNNTGG 1 cut(s) 602
BsuRI GGCC 6 cut(s) 38, 180, 197, 309, 423, 594
BtsCI GGATG 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 2 cut(s) 585, 681
Cac8I GCNNGC 1 cut(s) 178
CfoI GCGC 1 cut(s) 515
Cfr10I RCCGGY 2 cut(s) 176, 419
Cfr13I GGNCC 2 cut(s) 179, 780
Csp6I GTAC 2 cut(s) 146, 790
CviAII CATG 3 cut(s) 45, 236, 550
CviQI GTAC 2 cut(s) 146, 790
DdeI CTNAG 4 cut(s) 85, 449, 753, 890
DinI GGCGCC 1 cut(s) 514
EaeI YGGCCR 2 cut(s) 195, 307
Eam1104I CTCTTC 2 cut(s) 30, 56
EarI CTCTTC 2 cut(s) 30, 56
Ecl136II GAGCTC 1 cut(s) 583
Eco24I GRGCYC 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 780
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 4 cut(s) 246, 524, 609, 672
EcoICRI GAGCTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 585
EgeI GGCGCC 1 cut(s) 514
EheI GGCGCC 1 cut(s) 514
FaeI CATG 3 cut(s) 48, 239, 553
FaiI YATR 8 cut(s) 41, 46, 237, 242, 551, 708, 773, 810
FaqI GGGAC 5 cut(s) 265, 299, 392, 520, 793
FatI CATG 3 cut(s) 44, 235, 549
FauI CCCGC 2 cut(s) 341, 476
Fnu4HI GCNGC 1 cut(s) 664
FokI GGATG 1 cut(s) 201
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 1 cut(s) 664
FspBI CTAG 3 cut(s) 59, 645, 898
GlaI GCGC 1 cut(s) 514
GluI GCNGC 1 cut(s) 664
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 6 cut(s) 38, 180, 197, 309, 423, 594
HapII CCGG 3 cut(s) 177, 420, 783
HhaI GCGC 1 cut(s) 515
Hin1I GRCGYC 1 cut(s) 513
Hin1II CATG 3 cut(s) 48, 239, 553
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HinfI GANTC 4 cut(s) 93, 206, 387, 755
HpaII CCGG 3 cut(s) 177, 420, 783
HphI GGTGA 1 cut(s) 409
Hpy166II GTNNAC 3 cut(s) 508, 736, 862
Hpy188I TCNGA 5 cut(s) 428, 452, 543, 652, 754
Hpy188III TCNNGA 2 cut(s) 505, 872
Hpy8I GTNNAC 3 cut(s) 508, 736, 862
Hpy99I CGWCG 3 cut(s) 164, 506, 644
HpyAV CCTTC 5 cut(s) 65, 270, 421, 548, 584
HpyCH4III ACNGT 6 cut(s) 145, 193, 534, 568, 868, 883
HpyCH4IV ACGT 2 cut(s) 405, 639
HpyCH4V TGCA 3 cut(s) 235, 686, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 194, 618, 683
HpyF3I CTNAG 4 cut(s) 85, 449, 753, 890
HpySE526I ACGT 2 cut(s) 405, 639
Hsp92I GRCGYC 1 cut(s) 513
Hsp92II CATG 3 cut(s) 48, 239, 553
HspAI GCGC 1 cut(s) 513
KasI GGCGCC 1 cut(s) 512
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
LmnI GCTCC 2 cut(s) 335, 660
Lsp1109I GCAGC 1 cut(s) 675
MaeI CTAG 3 cut(s) 59, 645, 898
MaeII ACGT 2 cut(s) 405, 639
MaeIII GTNAC 3 cut(s) 274, 286, 893
MboII GAAGA 5 cut(s) 17, 43, 116, 406, 665
MhlI GDGCHC 1 cut(s) 585
MlsI TGGCCA 2 cut(s) 197, 309
MluCI AATT 2 cut(s) 18, 875
MluNI TGGCCA 2 cut(s) 197, 309
Mly113I GGCGCC 1 cut(s) 513
MlyI GAGTC 3 cut(s) 102, 215, 764
Mox20I TGGCCA 2 cut(s) 197, 309
MroNI GCCGGC 1 cut(s) 176
MscI TGGCCA 2 cut(s) 197, 309
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 686
Msp20I TGGCCA 2 cut(s) 197, 309
MspI CCGG 3 cut(s) 177, 420, 783
MspR9I CCNGG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 360
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 4 cut(s) 173, 194, 618, 683
NaeI GCCGGC 1 cut(s) 178
NarI GGCGCC 1 cut(s) 513
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 3 cut(s) 48, 239, 553
NlaIV GGNNCC 4 cut(s) 166, 497, 514, 781
NmuCI GTSAC 2 cut(s) 274, 286
NspI RCATGY 1 cut(s) 48
PctI GAATGC 1 cut(s) 360
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 387
PkrI GCNGC 1 cut(s) 665
PleI GAGTC 3 cut(s) 101, 214, 763
PluTI GGCGCC 1 cut(s) 516
PpsI GAGTC 3 cut(s) 101, 214, 763
Psp124BI GAGCTC 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 4 cut(s) 166, 497, 514, 781
PspPI GGNCC 2 cut(s) 179, 780
RsaI GTAC 2 cut(s) 147, 791
RsaNI GTAC 2 cut(s) 146, 790
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 1 cut(s) 664
Sau96I GGNCC 2 cut(s) 179, 780
ScaI AGTACT 1 cut(s) 791
SchI GAGTC 3 cut(s) 102, 215, 764
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 1 cut(s) 585
SfoI GGCGCC 1 cut(s) 514
SinI GGWCC 1 cut(s) 780
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 2 cut(s) 18, 875
SsiI CCGC 4 cut(s) 186, 218, 348, 483
SspDI GGCGCC 1 cut(s) 512
SspMI CTAG 3 cut(s) 59, 645, 898
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 1 cut(s) 526
TaaI ACNGT 6 cut(s) 145, 193, 534, 568, 868, 883
TaiI ACGT 2 cut(s) 408, 642
TasI AATT 2 cut(s) 18, 875
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 1 cut(s) 387
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TscAI CASTG 2 cut(s) 592, 688
TseFI GTSAC 2 cut(s) 274, 286
TseI GCWGC 1 cut(s) 663
Tsp45I GTSAC 2 cut(s) 274, 286
TspDTI ATGAA 2 cut(s) 117, 466
TspRI CASTG 2 cut(s) 592, 688
VpaK11BI GGWCC 1 cut(s) 780
XapI RAATTY 1 cut(s) 875
XceI RCATGY 1 cut(s) 48
XspI CTAG 3 cut(s) 59, 645, 898
ZrmI AGTACT 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.