MD01G1167500.v1.1

NPR1/NIM1 like defence protein C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003115
Physical Location & Seq
Forward (+)
27206925 .. 27207530
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1167500.v1.1.491

Sequence Viewer

Length: 606 bp
ATGAAAGAGAACCGACATAGCTTTTCGTGTCGTTTCCCACAAACGGCGCCAAATGTTGATGCAGAAAATCGGAGGGGTCTTCCCGTCCACAGCTGCGTTCTGTCTGCGAGGAGCGAGTTTTTCAAGAACGTGTTCTCGACGAATGACAGAGGATCATCAACAGCGACGCTGTCGTCGTCGGTTGCAGCTCCTCGGTTTGAATTGAAGGAGCTCGCCAAGGAATATGACATCGGACTGGATGCGCTCCTCATCGTTTTGGGGTATTTGTACAGCAGGAAGCTCAGATCTCTGCCTAAAGGCGTTTGCGTTTGCGTGGACAATGCTTGCACATATGTCGCTTGCCGGCCGTTGGTTAATTTCATGGTGGAGGTTCTTTTGAACTATATTCATCAATCCATTGTTATCAATAGCTCCTTCATCGACCCCGACGGCGGGGCCCTGGTCGATCTCGTGGTGCTGGAAAGCCAAAGAGCTGCTAAGGCTCTAGAGGCGGAAGCGTTGCCCAAGGTGCGGCTGACAAAGATTGATCTCAAGTGGGTCTGCATCATCAGCAAGGGATGGGCCAGCCCGCTGCGGGGATTTATGAGAGAAGACCAGTACTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001666 GO:0002376 GO:0002682 GO:0002831 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009625 GO:0009627 GO:0009628 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010033 GO:0010112 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0014070 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031348 GO:0032101 GO:0032870 GO:0035690 GO:0036293 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048585 GO:0050776 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0080027 GO:0080090 GO:0080134 GO:0098542 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000022 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 BTB DUF3420 NPR1_like_C

Protein Analysis

202

Amino Acids

22.4

Weight (kDa)

8.68

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_2 PF14306 140 - 200 1.5e-14 PUA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 46
AciI CCGC 5 cut(s) 432, 491, 511, 569, 574
AclWI GGATC 1 cut(s) 160
AcoI YGGCCR 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 2 cut(s) 269, 599
AfiI CCNNNNNNNGG 7 cut(s) 43, 349, 431, 432, 510, 574, 575
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 4 cut(s) 124, 200, 205, 379
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 7 cut(s) 21, 93, 188, 211, 280, 411, 473
AluI AGCT 7 cut(s) 21, 93, 188, 211, 280, 411, 473
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 1 cut(s) 160
AoxI GGCC 3 cut(s) 344, 435, 561
ApaI GGGCCC 1 cut(s) 439
ApeKI GCWGC 4 cut(s) 93, 185, 473, 571
Asp700I GAANNNNTTC 1 cut(s) 131
AspLEI GCGC 2 cut(s) 49, 244
AspS9I GGNCC 3 cut(s) 435, 436, 561
BaeGI GKGCMC 1 cut(s) 439
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 2 cut(s) 213, 439
BauI CACGAG 1 cut(s) 449
BbsI GAAGAC 2 cut(s) 71, 597
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 80, 197, 460, 558
BccI CCATC 1 cut(s) 552
BceAI ACGGC 3 cut(s) 60, 331, 445
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciT130I CCWGG 1 cut(s) 440
BfaI CTAG 1 cut(s) 485
BfoI RGCGCY 1 cut(s) 50
BglII AGATCT 1 cut(s) 284
BisI GCNGC 5 cut(s) 94, 186, 474, 512, 572
BlsI GCNGC 5 cut(s) 95, 187, 475, 513, 573
BmcAI AGTACT 1 cut(s) 599
Bme1390I CCNGG 1 cut(s) 440
BmgT120I GGNCC 3 cut(s) 435, 436, 561
BmiI GGNNCC 3 cut(s) 48, 436, 437
BmrFI CCNGG 1 cut(s) 440
BmsI GCATC 3 cut(s) 49, 229, 552
BpiI GAAGAC 2 cut(s) 71, 597
Bpu10I CCTNAGC 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 515
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 191, 216, 438, 504
Bsc4I CCNNNNNNNGG 7 cut(s) 43, 349, 431, 432, 510, 574, 575
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 2 cut(s) 240, 595
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 4 cut(s) 191, 216, 438, 504
BseGI GGATG 2 cut(s) 244, 563
BseLI CCNNNNNNNGG 7 cut(s) 43, 349, 431, 432, 510, 574, 575
BseMII CTCAG 1 cut(s) 295
BseNI ACTGG 2 cut(s) 240, 595
BseRI GAGGAG 3 cut(s) 124, 180, 236
BseSI GKGCMC 1 cut(s) 439
BseX3I CGGCCG 1 cut(s) 344
BseXI GCAGC 4 cut(s) 80, 197, 460, 558
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 3 cut(s) 346, 437, 563
BshNI GGYRCC 1 cut(s) 46
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 1 cut(s) 343
BslI CCNNNNNNNGG 7 cut(s) 43, 349, 431, 432, 510, 574, 575
BsnI GGCC 3 cut(s) 346, 437, 563
Bsp120I GGGCCC 1 cut(s) 435
Bsp1286I GDGCHC 2 cut(s) 213, 439
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 4 cut(s) 152, 284, 445, 526
BspACI CCGC 5 cut(s) 432, 491, 511, 569, 574
BspANI GGCC 3 cut(s) 346, 437, 563
BspCNI CTCAG 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 48, 436, 437
BspPI GGATC 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 240, 595
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 4 cut(s) 191, 216, 438, 504
BssMI GATC 4 cut(s) 152, 284, 445, 526
BssNI GRCGYC 1 cut(s) 47
BssSI CACGAG 1 cut(s) 449
BssT1I CCWWGG 2 cut(s) 216, 504
Bst2BI CACGAG 1 cut(s) 449
Bst2UI CCWGG 1 cut(s) 440
BstACI GRCGYC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 267
BstC8I GCNNGC 6 cut(s) 213, 325, 340, 344, 565, 569
BstDEI CTNAG 2 cut(s) 281, 477
BstF5I GGATG 2 cut(s) 244, 563
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 2 cut(s) 49, 244
BstKTI GATC 4 cut(s) 155, 287, 448, 529
BstMBI GATC 4 cut(s) 152, 284, 445, 526
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 4 cut(s) 479, 488, 508, 549
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 439
BstV1I GCAGC 4 cut(s) 80, 197, 460, 558
BstV2I GAAGAC 2 cut(s) 71, 597
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 3 cut(s) 346, 437, 563
BtsCI GGATG 2 cut(s) 244, 563
Cac8I GCNNGC 6 cut(s) 213, 325, 340, 344, 565, 569
CfoI GCGC 2 cut(s) 49, 244
Cfr10I RCCGGY 1 cut(s) 342
Cfr13I GGNCC 3 cut(s) 435, 436, 561
CseI GACGC 1 cut(s) 175
Csp6I GTAC 2 cut(s) 268, 598
CviAII CATG 1 cut(s) 361
CviQI GTAC 2 cut(s) 268, 598
DdeI CTNAG 2 cut(s) 281, 477
DinI GGCGCC 1 cut(s) 48
DpnI GATC 4 cut(s) 154, 286, 447, 528
DpnII GATC 4 cut(s) 152, 284, 445, 526
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 344
EagI CGGCCG 1 cut(s) 344
EciI GGCGGA 1 cut(s) 506
Ecl136II GAGCTC 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 216, 504
Eco24I GRGCYC 2 cut(s) 213, 439
Eco52I CGGCCG 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 2 cut(s) 435, 436
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 216, 504
EcoT38I GRGCYC 2 cut(s) 213, 439
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 216, 504
FaeI CATG 1 cut(s) 364
FaiI YATR 8 cut(s) 18, 225, 331, 333, 362, 384, 584, 604
FatI CATG 1 cut(s) 360
FauI CCCGC 3 cut(s) 425, 567, 576
FauNDI CATATG 1 cut(s) 331
Fnu4HI GCNGC 5 cut(s) 94, 186, 474, 512, 572
FokI GGATG 2 cut(s) 251, 570
FriOI GRGCYC 2 cut(s) 213, 439
Fsp4HI GCNGC 5 cut(s) 94, 186, 474, 512, 572
FspBI CTAG 1 cut(s) 485
GlaI GCGC 2 cut(s) 48, 243
GluI GCNGC 5 cut(s) 94, 186, 474, 512, 572
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 3 cut(s) 346, 437, 563
HapII CCGG 1 cut(s) 343
HgaI GACGC 1 cut(s) 175
HhaI GCGC 2 cut(s) 49, 244
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 1 cut(s) 364
Hin6I GCGC 2 cut(s) 47, 242
HinP1I GCGC 2 cut(s) 47, 242
HpaII CCGG 1 cut(s) 343
Hpy166II GTNNAC 2 cut(s) 88, 316
Hpy188I TCNGA 3 cut(s) 72, 233, 284
Hpy188III TCNNGA 3 cut(s) 124, 136, 485
Hpy8I GTNNAC 2 cut(s) 88, 316
Hpy99I CGWCG 5 cut(s) 142, 169, 178, 181, 431
HpyAV CCTTC 2 cut(s) 199, 424
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 4 cut(s) 62, 185, 327, 543
HpyF10VI GCNNNNNNNGC 4 cut(s) 479, 488, 508, 549
HpyF3I CTNAG 2 cut(s) 281, 477
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 1 cut(s) 364
HspAI GCGC 2 cut(s) 47, 242
KasI GGCGCC 1 cut(s) 46
KroI GCCGGC 1 cut(s) 342
KroNI GCCGGC 1 cut(s) 344
Kzo9I GATC 4 cut(s) 152, 284, 445, 526
LmnI GCTCC 5 cut(s) 111, 193, 208, 249, 416
LpnPI CCDG 7 cut(s) 221, 259, 356, 425, 443, 452, 577
Lsp1109I GCAGC 4 cut(s) 80, 197, 460, 558
LweI GCATC 3 cut(s) 49, 229, 552
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 129
MalI GATC 4 cut(s) 154, 286, 447, 528
MboI GATC 4 cut(s) 152, 284, 445, 526
MboII GAAGA 2 cut(s) 71, 602
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 2 cut(s) 213, 439
MluCI AATT 2 cut(s) 200, 355
Mly113I GGCGCC 1 cut(s) 47
MnlI CCTC 7 cut(s) 66, 102, 143, 201, 257, 361, 481
MroNI GCCGGC 1 cut(s) 342
MroXI GAANNNNTTC 1 cut(s) 131
MseI TTAA 1 cut(s) 354
MspA1I CMGCKG 2 cut(s) 93, 571
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 4 cut(s) 479, 488, 508, 549
NaeI GCCGGC 1 cut(s) 344
NarI GGCGCC 1 cut(s) 47
NdeI CATATG 1 cut(s) 331
NdeII GATC 4 cut(s) 152, 284, 445, 526
NgoMIV GCCGGC 1 cut(s) 342
NlaIII CATG 1 cut(s) 364
NlaIV GGNNCC 3 cut(s) 48, 436, 437
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PdiI GCCGGC 1 cut(s) 344
PdmI GAANNNNTTC 1 cut(s) 131
PflFI GACNNNGTC 1 cut(s) 169
PkrI GCNGC 5 cut(s) 95, 187, 475, 513, 573
PluTI GGCGCC 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 3 cut(s) 48, 436, 437
PspOMI GGGCCC 1 cut(s) 435
PspPI GGNCC 3 cut(s) 435, 436, 561
PsuI RGATCY 1 cut(s) 284
PsyI GACNNNGTC 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 93
RsaI GTAC 2 cut(s) 269, 599
RsaNI GTAC 2 cut(s) 268, 598
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 5 cut(s) 94, 186, 474, 512, 572
Sau3AI GATC 4 cut(s) 152, 284, 445, 526
Sau96I GGNCC 3 cut(s) 435, 436, 561
ScaI AGTACT 1 cut(s) 599
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 2 cut(s) 213, 439
SfaNI GCATC 3 cut(s) 49, 229, 552
SfoI GGCGCC 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 2 cut(s) 200, 355
SsiI CCGC 5 cut(s) 432, 491, 511, 569, 574
SspDI GGCGCC 1 cut(s) 46
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 438
StyI CCWWGG 2 cut(s) 216, 504
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 137, 420, 444
TasI AATT 2 cut(s) 200, 355
TatI WGTACW 2 cut(s) 267, 597
TauI GCSGC 1 cut(s) 514
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TseI GCWGC 4 cut(s) 93, 185, 473, 571
TspDTI ATGAA 4 cut(s) 17, 349, 377, 406
Tth111I GACNNNGTC 1 cut(s) 169
XbaI TCTAGA 1 cut(s) 484
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 485
ZrmI AGTACT 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.