Rroxscaffold_2G00118110

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003115
Physical Location & Seq
Reverse (-)
49305809 .. 49307803
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118110.1

Sequence Viewer

Length: 1014 bp
ATGCACGCGATCGTATCGACGAGCTCTACAACTTTCATGAAGGAAGTTTTCCCAAATTCCCTTCCCTCTTGCCTCCCAAAGCCTCATGGCAACACAAAACTCAACTCCGCTGCCCCTCAAGGCCATAACATACCTATTCGTTCTAGCTCTTGCAATAACACACACCACCTCAAACCAGAGTCCCTGACGAGGAAACCGATACGCCTCAAACCCAAATACCTGTTTCCACCGTACCTCCTTCTGTGGTCGGTGCCACGTCAGCGGGCCCCTCCTCCTGTGGTTGGTGCCACATCAGCAGGGCCGAGCGCCATAGTGGCCAATGGAGAGTCCTACCATCCGCTTTCATTCTTTCTGCATGATATAATTGGTGGCACCAATCCCTCAGCGAGAGCTGTGACTGGGATTGTAACCAACCCAGCTGTCAATGGCCAAGTCCCCTTTGCCAAACCCAATGGGGCAGTTGTCCAAGGTGTGAATAATGGCATTCCCCAAAACAACAACAACAATGGTATCATCAACAATAACAATGTCCCCTTCTTCACTGGCCTCGGAGGAAACATCAACAACAACCTCAGACAAAACAACAACTTTATTGGCGGGAACAATGGGTTCCCCGTTGTGAATGGGGCCCAGCTCCCGCCAGGACAGTCCCTTCAGAAGCTCATGTTCGGTACACTCACGGTCTTTGACGATGAGCTCACTGAAGGGCATGAGCTTGGCTCTGGCTTGTTGGGCAAGGCACAAGGATTTTACGTTGCTAGTTCCGAAGATGGGAGCAGCCAGACCATCGCTTTCACTGCAATGTTTGAGAGTGGTGGGTATGCTGATAGCCTCACATTCTTTGGGGTTCACAGAGTAGCTGCTTCAGAGTCTCACCTTGCAGTTATGGGAGGGACCGGGAAGTACTTGAATGCAAAAGGGTATGCTTTGGTTAAGACCATTAATGGAGTGAATGAGCAAAATACTGATGGAATTGACACTGTTCTTCAGTTTACTGTGTATCTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

337

Amino Acids

35.72

Weight (kDa)

9.0

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 204 - 335 1.3e-28 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 250, 284, 371
AccII CGCG 1 cut(s) 8
AciI CCGC 5 cut(s) 108, 262, 338, 597, 638
AcoI YGGCCR 2 cut(s) 315, 427
AcsI RAATTY 1 cut(s) 55
AcuI CTGAAG 4 cut(s) 638, 723, 849, 971
AfaI GTAC 3 cut(s) 233, 673, 905
AfiI CCNNNNNNNGG 3 cut(s) 189, 281, 771
AgsI TTSAA 1 cut(s) 910
AjiI CACGTC 1 cut(s) 257
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 9 cut(s) 24, 147, 392, 419, 634, 661, 697, 715, 860
AluI AGCT 9 cut(s) 24, 147, 392, 419, 634, 661, 697, 715, 860
Alw21I GWGCWC 2 cut(s) 26, 699
Alw26I GTCTC 1 cut(s) 876
AoxI GGCC 7 cut(s) 121, 264, 299, 315, 427, 544, 627
ApaI GGGCCC 2 cut(s) 268, 631
ApeKI GCWGC 3 cut(s) 110, 777, 860
ApoI RAATTY 1 cut(s) 55
AseI ATTAAT 1 cut(s) 942
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 6 cut(s) 264, 265, 299, 627, 628, 894
AsuC2I CCSGG 1 cut(s) 898
AsuHPI GGTGA 1 cut(s) 866
AvaII GGWCC 1 cut(s) 894
BaeGI GKGCMC 2 cut(s) 268, 631
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BalI TGGCCA 2 cut(s) 317, 429
BanI GGYRCC 3 cut(s) 250, 284, 371
BanII GRGCYC 4 cut(s) 26, 268, 631, 699
Bbv12I GWGCWC 2 cut(s) 26, 699
BbvCI CCTCAGC 1 cut(s) 382
BbvI GCAGC 3 cut(s) 97, 789, 847
BccI CCATC 4 cut(s) 342, 764, 794, 962
BciT130I CCWGG 1 cut(s) 642
BcnI CCSGG 1 cut(s) 898
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 3 cut(s) 144, 759, 1012
BfoI RGCGCY 1 cut(s) 309
BglI GCCNNNNNGGC 1 cut(s) 314
BisI GCNGC 3 cut(s) 111, 778, 861
BlsI GCNGC 3 cut(s) 112, 779, 862
BmcAI AGTACT 1 cut(s) 905
Bme1390I CCNGG 2 cut(s) 642, 898
Bme18I GGWCC 1 cut(s) 894
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 6 cut(s) 264, 265, 299, 627, 628, 894
BmiI GGNNCC 9 cut(s) 252, 266, 267, 286, 373, 611, 628, 629, 895
BmrFI CCNGG 2 cut(s) 642, 898
BmrI ACTGGG 1 cut(s) 408
BmuI ACTGGG 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 704, 736
Bpu10I CCTNAGC 1 cut(s) 382
BpuEI CTTGAG 1 cut(s) 102
BpuMI CCSGG 1 cut(s) 898
BsaJI CCNNGG 2 cut(s) 466, 547
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 281, 771
Bse1I ACTGG 2 cut(s) 403, 547
Bse3DI GCAATG 1 cut(s) 807
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 2 cut(s) 466, 547
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 3 cut(s) 189, 281, 771
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 2 cut(s) 396, 586
BseNI ACTGG 2 cut(s) 403, 547
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 2 cut(s) 268, 631
BseXI GCAGC 3 cut(s) 97, 789, 847
BseYI CCCAGC 2 cut(s) 415, 630
Bsh1236I CGCG 1 cut(s) 8
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 7 cut(s) 123, 266, 301, 317, 429, 546, 629
BshNI GGYRCC 3 cut(s) 250, 284, 371
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 26, 699
BsiSI CCGG 1 cut(s) 897
BslFI GGGAC 5 cut(s) 166, 419, 515, 634, 907
BslI CCNNNNNNNGG 3 cut(s) 189, 281, 771
BsmAI GTCTC 1 cut(s) 876
BsmFI GGGAC 5 cut(s) 166, 419, 515, 634, 907
BsmI GAATGC 2 cut(s) 483, 916
BsnI GGCC 7 cut(s) 123, 266, 301, 317, 429, 546, 629
Bsp120I GGGCCC 2 cut(s) 264, 627
Bsp1286I GDGCHC 4 cut(s) 26, 268, 631, 699
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 5 cut(s) 108, 262, 338, 597, 638
BspANI GGCC 7 cut(s) 123, 266, 301, 317, 429, 546, 629
BspCNI CTCAG 2 cut(s) 395, 585
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 9 cut(s) 252, 266, 267, 286, 373, 611, 628, 629, 895
BspT107I GGYRCC 3 cut(s) 250, 284, 371
BsrDI GCAATG 1 cut(s) 807
BsrI ACTGG 2 cut(s) 403, 547
BssECI CCNNGG 2 cut(s) 466, 547
BssMI GATC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 5 cut(s) 231, 648, 682, 982, 997
BstC8I GCNNGC 2 cut(s) 6, 264
BstDEI CTNAG 3 cut(s) 382, 572, 1004
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 1 cut(s) 12
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 1 cut(s) 9
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 5 cut(s) 259, 293, 314, 732, 797
BstNI CCWGG 1 cut(s) 642
BstSCI CCNGG 2 cut(s) 640, 896
BstSLI GKGCMC 2 cut(s) 268, 631
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 97, 789, 847
BsuRI GGCC 7 cut(s) 123, 266, 301, 317, 429, 546, 629
BtgZI GCGATG 1 cut(s) 772
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 4 cut(s) 540, 699, 795, 978
Cac8I GCNNGC 2 cut(s) 6, 264
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 308
Cfr13I GGNCC 6 cut(s) 264, 265, 299, 627, 628, 894
Csp6I GTAC 3 cut(s) 232, 672, 904
CviAII CATG 5 cut(s) 37, 86, 356, 664, 710
CviQI GTAC 3 cut(s) 232, 672, 904
DdeI CTNAG 3 cut(s) 382, 572, 1004
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
EaeI YGGCCR 2 cut(s) 315, 427
Ecl136II GAGCTC 2 cut(s) 24, 697
Eco130I CCWWGG 1 cut(s) 466
Eco24I GRGCYC 4 cut(s) 26, 268, 631, 699
Eco47I GGWCC 1 cut(s) 894
Eco53kI GAGCTC 2 cut(s) 24, 697
Eco57I CTGAAG 4 cut(s) 638, 723, 849, 971
EcoICRI GAGCTC 2 cut(s) 24, 697
EcoO109I RGGNCCY 2 cut(s) 265, 627
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 466
EcoT38I GRGCYC 4 cut(s) 26, 268, 631, 699
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 5 cut(s) 40, 89, 359, 667, 713
FaqI GGGAC 5 cut(s) 166, 419, 515, 634, 907
FatI CATG 5 cut(s) 36, 85, 355, 663, 709
FauI CCCGC 3 cut(s) 255, 590, 645
Fnu4HI GCNGC 3 cut(s) 111, 778, 861
FokI GGATG 1 cut(s) 321
FriOI GRGCYC 4 cut(s) 26, 268, 631, 699
Fsp4HI GCNGC 3 cut(s) 111, 778, 861
FspBI CTAG 3 cut(s) 144, 759, 1012
GlaI GCGC 1 cut(s) 307
GluI GCNGC 3 cut(s) 111, 778, 861
GsaI CCCAGC 2 cut(s) 419, 634
HaeII RGCGCY 1 cut(s) 309
HaeIII GGCC 7 cut(s) 123, 266, 301, 317, 429, 546, 629
HapII CCGG 1 cut(s) 897
HhaI GCGC 1 cut(s) 308
Hin1II CATG 5 cut(s) 40, 89, 359, 667, 713
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HinfI GANTC 3 cut(s) 179, 326, 869
HpaII CCGG 1 cut(s) 897
HphI GGTGA 1 cut(s) 866
Hpy166II GTNNAC 3 cut(s) 674, 850, 993
Hpy188I TCNGA 5 cut(s) 551, 575, 657, 766, 868
Hpy188III TCNNGA 1 cut(s) 37
Hpy8I GTNNAC 3 cut(s) 674, 850, 993
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 6 cut(s) 34, 71, 248, 544, 662, 698
HpyCH4III ACNGT 5 cut(s) 231, 648, 682, 982, 997
HpyCH4IV ACGT 2 cut(s) 256, 753
HpyCH4V TGCA 6 cut(s) 4, 153, 355, 800, 881, 914
HpyF10VI GCNNNNNNNGC 5 cut(s) 259, 293, 314, 732, 797
HpyF3I CTNAG 3 cut(s) 382, 572, 1004
HpySE526I ACGT 2 cut(s) 256, 753
Hsp92II CATG 5 cut(s) 40, 89, 359, 667, 713
HspAI GCGC 1 cut(s) 306
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 2 cut(s) 639, 774
Lsp1109I GCAGC 3 cut(s) 97, 789, 847
MaeI CTAG 3 cut(s) 144, 759, 1012
MaeII ACGT 2 cut(s) 256, 753
MaeIII GTNAC 3 cut(s) 394, 406, 1007
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MboII GAAGA 3 cut(s) 529, 779, 977
MhlI GDGCHC 4 cut(s) 26, 268, 631, 699
MlsI TGGCCA 2 cut(s) 317, 429
MluCI AATT 3 cut(s) 55, 363, 972
MluNI TGGCCA 2 cut(s) 317, 429
MlyI GAGTC 3 cut(s) 188, 335, 878
Mox20I TGGCCA 2 cut(s) 317, 429
MscI TGGCCA 2 cut(s) 317, 429
MseI TTAA 2 cut(s) 933, 942
MslI CAYNNNNRTG 1 cut(s) 800
Msp20I TGGCCA 2 cut(s) 317, 429
MspA1I CMGCKG 3 cut(s) 110, 262, 419
MspI CCGG 1 cut(s) 897
MspR9I CCNGG 2 cut(s) 642, 898
Mva1269I GAATGC 2 cut(s) 483, 916
MvaI CCWGG 1 cut(s) 642
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 5 cut(s) 259, 293, 314, 732, 797
NciI CCSGG 1 cut(s) 898
NdeII GATC 1 cut(s) 9
NlaIII CATG 5 cut(s) 40, 89, 359, 667, 713
NlaIV GGNNCC 9 cut(s) 252, 266, 267, 286, 373, 611, 628, 629, 895
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 394
PagI TCATGA 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PctI GAATGC 2 cut(s) 483, 916
PkrI GCNGC 3 cut(s) 112, 779, 862
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 3 cut(s) 187, 334, 877
PpsI GAGTC 3 cut(s) 187, 334, 877
PshBI ATTAAT 1 cut(s) 942
Psp124BI GAGCTC 2 cut(s) 26, 699
Psp6I CCWGG 1 cut(s) 640
PspFI CCCAGC 2 cut(s) 415, 630
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 9 cut(s) 252, 266, 267, 286, 373, 611, 628, 629, 895
PspOMI GGGCCC 2 cut(s) 264, 627
PspPI GGNCC 6 cut(s) 264, 265, 299, 627, 628, 894
PvuI CGATCG 1 cut(s) 12
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 3 cut(s) 233, 673, 905
RsaNI GTAC 3 cut(s) 232, 672, 904
RseI CAYNNNNRTG 1 cut(s) 800
SacI GAGCTC 2 cut(s) 26, 699
SaqAI TTAA 2 cut(s) 933, 942
SatI GCNGC 3 cut(s) 111, 778, 861
Sau3AI GATC 1 cut(s) 9
Sau96I GGNCC 6 cut(s) 264, 265, 299, 627, 628, 894
ScaI AGTACT 1 cut(s) 905
SchI GAGTC 3 cut(s) 188, 335, 878
ScrFI CCNGG 2 cut(s) 642, 898
SduI GDGCHC 4 cut(s) 26, 268, 631, 699
SinI GGWCC 1 cut(s) 894
SmiMI CAYNNNNRTG 1 cut(s) 800
SmlI CTYRAG 1 cut(s) 117
SmoI CTYRAG 1 cut(s) 117
Sse9I AATT 3 cut(s) 55, 363, 972
SsiI CCGC 5 cut(s) 108, 262, 338, 597, 638
SspMI CTAG 3 cut(s) 144, 759, 1012
SstI GAGCTC 2 cut(s) 26, 699
StyD4I CCNGG 2 cut(s) 640, 896
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 5 cut(s) 231, 648, 682, 982, 997
TaiI ACGT 2 cut(s) 259, 756
TaqI TCGA 1 cut(s) 17
TasI AATT 3 cut(s) 55, 363, 972
TatI WGTACW 1 cut(s) 903
Tru1I TTAA 2 cut(s) 933, 942
Tru9I TTAA 2 cut(s) 933, 942
TscAI CASTG 4 cut(s) 547, 706, 802, 985
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 3 cut(s) 110, 777, 860
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 3 cut(s) 25, 53, 333
TspRI CASTG 4 cut(s) 547, 706, 802, 985
VpaK11BI GGWCC 1 cut(s) 894
VspI ATTAAT 1 cut(s) 942
XapI RAATTY 1 cut(s) 55
XspI CTAG 3 cut(s) 144, 759, 1012
ZrmI AGTACT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.