RLG00000027425

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003115
Physical Location & Seq
Forward (+)
11558176 .. 11559057
882 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027425

Sequence Viewer

Length: 882 bp
ATGGAAGCTCACAAAATGTTCTCCGTCACCCACCTACTATTCCTCACCCTCACCATTTTATCTGCCACCTCAGCGCGAACTCTCGAGGAGCAGCCACTGGTTCCTGTTGTGCCTCCGGTGGTGTCGAATACAATTGGCACACCTTCCATCAATGCTGCACCAGTGGTCACACCTTCAGCTTCACAGCCTGCCACGGTGGCTGATGCTGATGACCACCATTCCTTAGTCTTCTTCCTGCACGACATTCTAGGTGGCACAAACCCCTCAGCTAGAGCCATGACAGGGATAGTCAATAGCCCTGCAGTCAATGGACAAGTACCATTTGCCAAACCAAATGGAGCAGTCCTGCCATTTAACAATGGAATACCCCAGAACAACAACAACAACGGTATTCTAAGCAACAACAACATCCCGTTTCTCACTGGACTTAGTGGAGCAACGCAAAATGTGGTACAAAACAATAACAACAACAACAACAATTTCATCAATGGTGGGCAGCTCTTTCCAGGAGGCTCCACACTCCAAAACCTCATGTTCGGCACAATGAGTGTGTTCGATGATGAGCTAACTGAAGGGCATGAGCTCGGGTCTGGTTTGGTTGGCAAGGCACAAGGGTTCTATGTGGTGAGTTCTGAAGATGGTACGAGTCAGACTATGGCTTTCACTGCTATGTTTCAAAGTGGGGGTTATGCTGATAGCCTTAGCTTCTTTGGGGTCCATCGTGTGGCTGCGTCAGAGTCTCACTTGGCTATCATGGGTGGCACCGGAAAGTATGTTGATGCGAAAGGGTTTGCCATTGTTAAGACATTCCCGGCTACCAATGAGCATCAAACTGATGGAGCAGAGACCTTGTTGCAGTTCACTGTTTATATTACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

294

Amino Acids

30.79

Weight (kDa)

5.05

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 151 - 291 3.2e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 761
AccB7I CCANNNNNTGG 1 cut(s) 724
AccII CGCG 1 cut(s) 76
AcuI CTGAAG 3 cut(s) 159, 591, 654
AfaI GTAC 3 cut(s) 318, 453, 643
AfiI CCNNNNNNNGG 2 cut(s) 282, 724
AgsI TTSAA 1 cut(s) 677
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 7 cut(s) 8, 179, 269, 499, 565, 583, 705
AluI AGCT 7 cut(s) 8, 179, 269, 499, 565, 583, 705
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 2 cut(s) 744, 839
AlwNI CAGNNNCTG 1 cut(s) 97
Ama87I CYCGRG 2 cut(s) 83, 584
ApeKI GCWGC 4 cut(s) 91, 155, 496, 728
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 715
AsuC2I CCSGG 1 cut(s) 812
AsuHPI GGTGA 4 cut(s) 19, 37, 43, 637
AvaI CYCGRG 2 cut(s) 83, 584
AvaII GGWCC 1 cut(s) 715
BanI GGYRCC 1 cut(s) 761
BanII GRGCYC 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 585
BbvCI CCTCAGC 2 cut(s) 70, 265
BbvI GCAGC 4 cut(s) 103, 142, 508, 715
BccI CCATC 4 cut(s) 155, 632, 726, 830
BciT130I CCWGG 1 cut(s) 507
BcnI CCSGG 1 cut(s) 812
BcoDI GTCTC 2 cut(s) 744, 839
BfaI CTAG 3 cut(s) 248, 270, 880
BfmI CTRYAG 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 4 cut(s) 92, 156, 497, 729
BlsI GCNGC 4 cut(s) 93, 157, 498, 730
Bme1390I CCNGG 2 cut(s) 507, 812
Bme18I GGWCC 1 cut(s) 715
BmeT110I CYCGRG 2 cut(s) 83, 584
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 4 cut(s) 102, 514, 716, 763
BmrFI CCNGG 2 cut(s) 507, 812
BmsI GCATC 3 cut(s) 193, 769, 835
BpiI GAAGAC 1 cut(s) 220
Bpu10I CCTNAGC 3 cut(s) 70, 265, 701
BpuMI CCSGG 1 cut(s) 812
BsaI GGTCTC 1 cut(s) 839
BsaJI CCNNGG 1 cut(s) 192
BsaWI WCCGGW 2 cut(s) 115, 764
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 724
Bse1I ACTGG 3 cut(s) 102, 161, 427
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 282, 724
BseMII CTCAG 2 cut(s) 84, 279
BseNI ACTGG 3 cut(s) 102, 161, 427
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 4 cut(s) 103, 142, 508, 715
BsgI GTGCAG 2 cut(s) 141, 221
Bsh1236I CGCG 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 761
BsiHKAI GWGCWC 1 cut(s) 585
BsiHKCI CYCGRG 2 cut(s) 83, 584
BsiSI CCGG 3 cut(s) 116, 765, 812
BslI CCNNNNNNNGG 2 cut(s) 282, 724
BsmAI GTCTC 2 cut(s) 744, 839
Bso31I GGTCTC 1 cut(s) 839
BsoBI CYCGRG 2 cut(s) 83, 584
Bsp1286I GDGCHC 1 cut(s) 585
BspCNI CTCAG 2 cut(s) 83, 278
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 102, 514, 716, 763
BspMAI CTGCAG 1 cut(s) 304
BspT107I GGYRCC 1 cut(s) 761
BspTNI GGTCTC 1 cut(s) 839
BsrI ACTGG 3 cut(s) 102, 161, 427
BssECI CCNNGG 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 196, 389, 865
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 6 cut(s) 70, 223, 265, 395, 428, 701
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 76
BstMAI GTCTC 2 cut(s) 744, 839
BstMWI GCNNNNNNNGC 3 cut(s) 71, 197, 665
BstNI CCWGG 1 cut(s) 507
BstSCI CCNGG 2 cut(s) 505, 810
BstSFI CTRYAG 1 cut(s) 300
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 4 cut(s) 103, 142, 508, 715
BstV2I GAAGAC 1 cut(s) 220
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 5 cut(s) 95, 168, 420, 663, 861
Cac8I GCNNGC 1 cut(s) 189
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 715
CseI GACGC 1 cut(s) 720
Csp6I GTAC 3 cut(s) 317, 452, 642
CviAII CATG 4 cut(s) 277, 532, 578, 754
CviQI GTAC 3 cut(s) 317, 452, 642
DdeI CTNAG 6 cut(s) 70, 223, 265, 395, 428, 701
Ecl136II GAGCTC 1 cut(s) 583
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 839
Eco47I GGWCC 1 cut(s) 715
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 3 cut(s) 159, 591, 654
Eco88I CYCGRG 2 cut(s) 83, 584
EcoICRI GAGCTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 585
FaeI CATG 4 cut(s) 280, 535, 581, 757
FatI CATG 4 cut(s) 276, 531, 577, 753
Fnu4HI GCNGC 4 cut(s) 92, 156, 497, 729
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 4 cut(s) 92, 156, 497, 729
FspBI CTAG 3 cut(s) 248, 270, 880
GlaI GCGC 1 cut(s) 75
GluI GCNGC 4 cut(s) 92, 156, 497, 729
HapII CCGG 3 cut(s) 116, 765, 812
HgaI GACGC 1 cut(s) 720
HhaI GCGC 1 cut(s) 76
Hin1II CATG 4 cut(s) 280, 535, 581, 757
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 2 cut(s) 646, 737
HpaII CCGG 3 cut(s) 116, 765, 812
HphI GGTGA 4 cut(s) 19, 37, 43, 637
Hpy166II GTNNAC 1 cut(s) 861
Hpy188I TCNGA 3 cut(s) 634, 651, 736
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 1 cut(s) 861
HpyAV CCTTC 3 cut(s) 153, 183, 566
HpyCH4III ACNGT 3 cut(s) 196, 389, 865
HpyCH4V TGCA 4 cut(s) 158, 238, 302, 856
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 197, 665
HpyF3I CTNAG 6 cut(s) 70, 223, 265, 395, 428, 701
Hsp92II CATG 4 cut(s) 280, 535, 581, 757
HspAI GCGC 1 cut(s) 74
LmnI GCTCC 5 cut(s) 88, 338, 434, 518, 839
Lsp1109I GCAGC 4 cut(s) 103, 142, 508, 715
LweI GCATC 3 cut(s) 193, 769, 835
MaeI CTAG 3 cut(s) 248, 270, 880
MaeIII GTNAC 2 cut(s) 25, 166
MboII GAAGA 3 cut(s) 220, 223, 647
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 2 cut(s) 132, 478
MlyI GAGTC 2 cut(s) 655, 746
MnlI CCTC 8 cut(s) 53, 59, 79, 79, 123, 274, 503, 539
MseI TTAA 2 cut(s) 354, 801
MslI CAYNNNNRTG 1 cut(s) 668
MspI CCGG 3 cut(s) 116, 765, 812
MspR9I CCNGG 2 cut(s) 507, 812
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 3 cut(s) 71, 197, 665
NciI CCSGG 1 cut(s) 812
NlaIII CATG 4 cut(s) 280, 535, 581, 757
NlaIV GGNNCC 4 cut(s) 102, 514, 716, 763
NmuCI GTSAC 2 cut(s) 25, 166
PaeR7I CTCGAG 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 724
PfoI TCCNGGA 1 cut(s) 505
PkrI GCNGC 4 cut(s) 93, 157, 498, 730
PleI GAGTC 2 cut(s) 654, 745
PpsI GAGTC 2 cut(s) 654, 745
Psp124BI GAGCTC 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 4 cut(s) 102, 514, 716, 763
PspPI GGNCC 1 cut(s) 715
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 97
RsaI GTAC 3 cut(s) 318, 453, 643
RsaNI GTAC 3 cut(s) 317, 452, 642
RseI CAYNNNNRTG 1 cut(s) 668
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 2 cut(s) 354, 801
SatI GCNGC 4 cut(s) 92, 156, 497, 729
Sau96I GGNCC 1 cut(s) 715
SchI GAGTC 2 cut(s) 655, 746
ScrFI CCNGG 2 cut(s) 507, 812
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 3 cut(s) 193, 769, 835
SfcI CTRYAG 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 715
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 2 cut(s) 132, 478
SspMI CTAG 3 cut(s) 248, 270, 880
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 505, 810
TaaI ACNGT 3 cut(s) 196, 389, 865
TaqI TCGA 3 cut(s) 84, 125, 555
TasI AATT 2 cut(s) 132, 478
Tru1I TTAA 2 cut(s) 354, 801
Tru9I TTAA 2 cut(s) 354, 801
TscAI CASTG 5 cut(s) 102, 168, 427, 670, 868
TseFI GTSAC 2 cut(s) 25, 166
TseI GCWGC 4 cut(s) 91, 155, 496, 728
Tsp45I GTSAC 2 cut(s) 25, 166
TspDTI ATGAA 1 cut(s) 472
TspGWI ACGGA 1 cut(s) 13
TspRI CASTG 5 cut(s) 102, 168, 427, 670, 868
Van91I CCANNNNNTGG 1 cut(s) 724
VpaK11BI GGWCC 1 cut(s) 715
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 3 cut(s) 248, 270, 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.