MD11G1115800.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003115
Physical Location & Seq
Forward (+)
10459301 .. 10460230
930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1115800.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCAACCCGGAAATCGGTTCCCTTGCCCTTCAAGCTCACAATCTGGCTACTTTTTCTAGCCCTTGCCATTCCTTGCGCCACTTGCGCTCGAATCCTCGATGAAGAAAGCCCTGCAGCCCCTGAAGAGCCTGATACAACTCAAACACAAATACCTGTTTCCAATGTGCCACCTCCTCTGGTGGCTACAAATCCAGCAGGCCCTGCTGCAGCTACAAGTGCCACAGTTGGCAACACAGCATCCTACCATCCGCTAACCTTCTTCTTGCATGACATTATCGGAGGATCAAACCCCTCAGCGAGAGCTGTCACTGGAATTGTGGCCAACCCGGCAGTCAGCGGTCAAATCCCTTTTGCCAAACCCAATGGAGCAAACATCCCAATTGGGAATGGCATTCCTCAAAACAGCAACAACAATGGAATCATCAACAACAACAACGTCCCCTTCTTCACCGGCCTTGGTGGAAACACTGGCAACAACTTGGTCCAAAACAATGGTAACAACAACATCATTGGTGGGAATGGGTTTCCGTACATTAACGGGGCTCAGCTCCCTCCTGGAATCACCCTTCAGAAGCTTATGTTTGGGACACTAACAGTTTTCGACGATGAGCTGACTGAAGGGCATGAGCTTGGTTCTGGCTTGCTCGGCAAGGCACAAGGGTTTTACGTGGCGAGTTCTGAAGATGGGAGCAGCCAGACCATTGCATTCACAGCCATGTTTCAGAGTGGTGGTTATGCTGATAGCCTCACCTTCTTCGGTGTGCATCGGGTGGCGGTGTCAGAGTCTCATCTTGCAGTCATGGGAGGAACTGGGAGGTATCTCAATGCAAAGGGTTATGCTCTTGTTAAGACTATTCCTGCAACAAATCAGCCCAACACTGATGGAGTTGACACTGTGCTGGAGTTTACTACGTATCTCACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

310

Amino Acids

32.11

Weight (kDa)

5.1

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 187 - 306 1.4e-26 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 251, 339, 776
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 1 cut(s) 321
AcuI CTGAAG 4 cut(s) 144, 554, 639, 702
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 1 cut(s) 16
AgsI TTSAA 1 cut(s) 34
AjnI CCWGG 1 cut(s) 556
AluBI AGCT 7 cut(s) 37, 212, 305, 550, 577, 613, 631
AluI AGCT 7 cut(s) 37, 212, 305, 550, 577, 613, 631
Alw26I GTCTC 1 cut(s) 792
AlwI GGATC 1 cut(s) 292
AlwNI CAGNNNCTG 2 cut(s) 122, 203
AoxI GGCC 3 cut(s) 199, 321, 454
ApeKI GCWGC 4 cut(s) 116, 206, 209, 693
AspLEI GCGC 2 cut(s) 80, 89
AspS9I GGNCC 2 cut(s) 200, 484
AsuC2I CCSGG 2 cut(s) 10, 329
AsuHPI GGTGA 3 cut(s) 442, 556, 742
AvaII GGWCC 1 cut(s) 484
BalI TGGCCA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 547
BbvCI CCTCAGC 1 cut(s) 295
BbvI GCAGC 4 cut(s) 128, 193, 221, 705
BccI CCATC 3 cut(s) 255, 680, 878
BciT130I CCWGG 1 cut(s) 558
BcnI CCSGG 2 cut(s) 10, 329
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 2 cut(s) 59, 928
BfmI CTRYAG 2 cut(s) 114, 207
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 4 cut(s) 117, 207, 210, 694
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 4 cut(s) 118, 208, 211, 695
Bme1390I CCNGG 3 cut(s) 10, 329, 558
Bme18I GGWCC 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 200, 484
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 3 cut(s) 10, 329, 558
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 2 cut(s) 248, 775
BmuI ACTGGG 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 923
Bpu10I CCTNAGC 1 cut(s) 295
Bpu1102I GCTNAGC 1 cut(s) 546
BpuMI CCSGG 2 cut(s) 10, 329
BsaAI YACGTR 2 cut(s) 670, 915
BsaJI CCNNGG 1 cut(s) 457
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 3 cut(s) 316, 475, 817
Bse3DI GCAATG 1 cut(s) 702
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 3 cut(s) 239, 247, 375
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 2 cut(s) 309, 560
BseNI ACTGG 3 cut(s) 316, 475, 817
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 4 cut(s) 128, 193, 221, 705
BshFI GGCC 3 cut(s) 201, 323, 456
BsiSI CCGG 3 cut(s) 10, 329, 453
BslFI GGGAC 2 cut(s) 425, 601
BslI CCNNNNNNNGG 1 cut(s) 16
BsmAI GTCTC 1 cut(s) 792
BsmFI GGGAC 2 cut(s) 425, 601
BsmI GAATGC 2 cut(s) 393, 707
BsnI GGCC 3 cut(s) 201, 323, 456
Bsp1286I GDGCHC 1 cut(s) 547
Bsp143I GATC 1 cut(s) 284
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 3 cut(s) 251, 339, 776
BspANI GGCC 3 cut(s) 201, 323, 456
BspCNI CTCAG 2 cut(s) 308, 559
BspLI GGNNCC 1 cut(s) 21
BspMAI CTGCAG 2 cut(s) 118, 211
BspPI GGATC 1 cut(s) 292
BspQI GCTCTTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 702
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 3 cut(s) 316, 475, 817
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 1 cut(s) 284
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 3 cut(s) 226, 598, 898
Bst6I CTCTTC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 203
BstBAI YACGTR 2 cut(s) 670, 915
BstC8I GCNNGC 2 cut(s) 199, 644
BstDEI CTNAG 2 cut(s) 295, 546
BstF5I GGATG 3 cut(s) 239, 247, 375
BstHHI GCGC 2 cut(s) 80, 89
BstKTI GATC 1 cut(s) 287
BstMAI GTCTC 1 cut(s) 792
BstMBI GATC 1 cut(s) 284
BstMWI GCNNNNNNNGC 8 cut(s) 34, 84, 86, 203, 218, 329, 648, 713
BstNI CCWGG 1 cut(s) 558
BstSCI CCNGG 3 cut(s) 8, 327, 556
BstSFI CTRYAG 2 cut(s) 114, 207
BstSNI TACGTA 1 cut(s) 915
BstV1I GCAGC 4 cut(s) 128, 193, 221, 705
BstXI CCANNNNNNTGG 1 cut(s) 494
BsuRI GGCC 3 cut(s) 201, 323, 456
BtsCI GGATG 3 cut(s) 239, 247, 375
BtsIMutI CAGTG 4 cut(s) 309, 468, 879, 894
Cac8I GCNNGC 2 cut(s) 199, 644
CaiI CAGNNNCTG 2 cut(s) 122, 203
CfoI GCGC 2 cut(s) 80, 89
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 200, 484
Csp6I GTAC 1 cut(s) 532
CviAII CATG 4 cut(s) 269, 626, 718, 802
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 2 cut(s) 295, 546
DpnI GATC 1 cut(s) 286
DpnII GATC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco105I TACGTA 1 cut(s) 915
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 547
Eco47I GGWCC 1 cut(s) 484
Eco57I CTGAAG 4 cut(s) 144, 554, 639, 702
EcoO109I RGGNCCY 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 4 cut(s) 272, 629, 721, 805
FaiI YATR 7 cut(s) 270, 581, 627, 719, 738, 803, 840
FaqI GGGAC 2 cut(s) 425, 601
FatI CATG 4 cut(s) 268, 625, 717, 801
Fnu4HI GCNGC 4 cut(s) 117, 207, 210, 694
FokI GGATG 3 cut(s) 226, 234, 362
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 4 cut(s) 117, 207, 210, 694
FspBI CTAG 2 cut(s) 59, 928
GlaI GCGC 2 cut(s) 79, 88
GluI GCNGC 4 cut(s) 117, 207, 210, 694
GsuI CTGGAG 1 cut(s) 923
HaeIII GGCC 3 cut(s) 201, 323, 456
HapII CCGG 3 cut(s) 10, 329, 453
HhaI GCGC 2 cut(s) 80, 89
Hin1II CATG 4 cut(s) 272, 629, 721, 805
Hin6I GCGC 2 cut(s) 78, 87
HinP1I GCGC 2 cut(s) 78, 87
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 4 cut(s) 93, 420, 561, 785
HpaII CCGG 3 cut(s) 10, 329, 453
HphI GGTGA 3 cut(s) 442, 556, 742
Hpy166II GTNNAC 2 cut(s) 892, 909
Hpy188I TCNGA 5 cut(s) 281, 573, 682, 726, 784
Hpy8I GTNNAC 2 cut(s) 892, 909
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 6 cut(s) 40, 268, 454, 578, 614, 763
HpyCH4III ACNGT 3 cut(s) 226, 598, 898
HpyCH4IV ACGT 3 cut(s) 438, 669, 914
HpyCH4V TGCA 8 cut(s) 116, 209, 268, 707, 766, 797, 830, 863
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 84, 86, 203, 218, 329, 648, 713
HpyF3I CTNAG 2 cut(s) 295, 546
HpySE526I ACGT 3 cut(s) 438, 669, 914
Hsp92II CATG 4 cut(s) 272, 629, 721, 805
HspAI GCGC 2 cut(s) 78, 87
Kzo9I GATC 1 cut(s) 284
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 368, 555, 690
Lsp1109I GCAGC 4 cut(s) 128, 193, 221, 705
LweI GCATC 2 cut(s) 248, 775
MaeI CTAG 2 cut(s) 59, 928
MaeII ACGT 3 cut(s) 438, 669, 914
MaeIII GTNAC 2 cut(s) 307, 497
MalI GATC 1 cut(s) 286
MboI GATC 1 cut(s) 284
MboII GAAGA 6 cut(s) 116, 137, 253, 439, 695, 748
MfeI CAATTG 1 cut(s) 381
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 2 cut(s) 315, 381
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 1 cut(s) 794
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MseI TTAA 2 cut(s) 537, 849
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 3 cut(s) 10, 329, 453
MspR9I CCNGG 3 cut(s) 10, 329, 558
MunI CAATTG 1 cut(s) 381
Mva1269I GAATGC 2 cut(s) 393, 707
MvaI CCWGG 1 cut(s) 558
MwoI GCNNNNNNNGC 8 cut(s) 34, 84, 86, 203, 218, 329, 648, 713
NciI CCSGG 2 cut(s) 10, 329
NdeII GATC 1 cut(s) 284
NlaIII CATG 4 cut(s) 272, 629, 721, 805
NlaIV GGNNCC 1 cut(s) 21
NmeAIII GCCGAG 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 120
PctI GAATGC 2 cut(s) 393, 707
PfeI GAWTC 3 cut(s) 93, 420, 561
PfoI TCCNGGA 1 cut(s) 556
PkrI GCNGC 4 cut(s) 118, 208, 211, 695
PleI GAGTC 1 cut(s) 793
PpsI GAGTC 1 cut(s) 793
Ppu21I YACGTR 2 cut(s) 670, 915
Psp6I CCWGG 1 cut(s) 556
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 2 cut(s) 200, 484
PstI CTGCAG 2 cut(s) 118, 211
PstNI CAGNNNCTG 2 cut(s) 122, 203
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
RseI CAYNNNNRTG 1 cut(s) 716
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 537, 849
SatI GCNGC 4 cut(s) 117, 207, 210, 694
Sau3AI GATC 1 cut(s) 284
Sau96I GGNCC 2 cut(s) 200, 484
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 3 cut(s) 10, 329, 558
SduI GDGCHC 1 cut(s) 547
SfaNI GCATC 2 cut(s) 248, 775
SfcI CTRYAG 2 cut(s) 114, 207
SinI GGWCC 1 cut(s) 484
SmiMI CAYNNNNRTG 1 cut(s) 716
SnaBI TACGTA 1 cut(s) 915
Sse9I AATT 2 cut(s) 315, 381
SsiI CCGC 3 cut(s) 251, 339, 776
SspMI CTAG 2 cut(s) 59, 928
StyD4I CCNGG 3 cut(s) 8, 327, 556
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 3 cut(s) 226, 598, 898
TaiI ACGT 3 cut(s) 441, 672, 917
TaqI TCGA 3 cut(s) 91, 99, 603
TasI AATT 2 cut(s) 315, 381
TfiI GAWTC 3 cut(s) 93, 420, 561
Tru1I TTAA 2 cut(s) 537, 849
Tru9I TTAA 2 cut(s) 537, 849
TscAI CASTG 4 cut(s) 316, 475, 886, 901
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 4 cut(s) 116, 206, 209, 693
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 117
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 4 cut(s) 316, 475, 886, 901
VpaK11BI GGWCC 1 cut(s) 484
XspI CTAG 2 cut(s) 59, 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.