Rh2CG305000

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003115
Physical Location & Seq
Forward (+)
36069907 .. 36099754
29848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG305000.1

Sequence Viewer

Length: 954 bp
ATGGCAACCCAAAACTCAACTCCGCTGCCCCTCAAGGCCATAACATACCTATTTCTTCTAGCTCTTGCAATAACACACACCACCTCAGCCAGAGTCCTTGATGAGGAAACCGATACGCCTCAAACCCAAATACCTGTTTCCACCGTACCTCCTTCTGTGGTCGGTGCCACGTCAGCGGGCCCTCCTCCTGTTGTCAGTGCCACGTCAGCAGGCCCGAGCGCCACAGTGGCGAATGGAGAGTCCTACCATCCGCTTTCATTCTTTCTGCATGATATAATTGGTGGCACCAATCCCTCAGCGAGAGCTGTGACTGGGATTGTGACCAACCCAGCTGTCAATGGCCAAGTCCCCTTTGCCAAACCCAATGGGGCAGTTGTCCAAGGCGGGAATAATGGCATTCCCCAAAACAACAACAACAATGGTATCATCAACAATAATGTCCCCTTCTTCACTGGCCTCGGAGGAAACATCAACAACAACCTCAGACAAAACAACAACAACTTTATTGGCGGGAACAATGGGTTCCCCGTTGTGAATGGGGCGCAGCTCCCGCCAGGACAGTCCCTTCAGAAGCTCATGTTCGGCACACTCACGGTCTTTGACGATGAGCTCACTGAAGGGCATGAGCTTGGCTCTGGCTTGTTGGGCAAGGCACAAGGATTTTACGTTTCTAGTTCCGAAGATGGGAGCAGCCAGACCATTGCTTTCACTGCTATGTTTGAGAGTGGTGGGTATGCTGATAGCCTCACATTCTTTGGGGTTCACAGAATAGCTGCTTCAGAGTCTCACCTTGCAGTTATGGGAGGAACAGGGAAGTACTTGAATGCAAAAGGGTATGCTTTGGTTAAGACCATTAATGGAGTGAATGAGCAAAATACTGATGGAATTGACACTACACAATTTCTATTGAAGAGACCATTAGATTTTCTTTACACGAATGCTCAAGTGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

317

Amino Acids

32.98

Weight (kDa)

5.25

Isoelectric Point (pI)

26.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 169 - 291 8.4e-28 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 164, 284
AciI CCGC 6 cut(s) 23, 176, 251, 384, 510, 551
AcoI YGGCCR 1 cut(s) 340
AcuI CTGAAG 3 cut(s) 551, 636, 762
AfaI GTAC 2 cut(s) 147, 818
AfiI CCNNNNNNNGG 2 cut(s) 103, 684
AgsI TTSAA 2 cut(s) 823, 910
AjiI CACGTC 2 cut(s) 171, 204
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 8 cut(s) 62, 305, 332, 547, 574, 610, 628, 773
AluI AGCT 8 cut(s) 62, 305, 332, 547, 574, 610, 628, 773
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 2 cut(s) 789, 907
Ama87I CYCGRG 1 cut(s) 214
AoxI GGCC 5 cut(s) 36, 178, 211, 340, 454
ApaI GGGCCC 1 cut(s) 182
ApeKI GCWGC 4 cut(s) 25, 544, 690, 773
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AseI ATTAAT 1 cut(s) 855
AspLEI GCGC 2 cut(s) 221, 544
AspS9I GGNCC 3 cut(s) 178, 179, 212
AsuHPI GGTGA 1 cut(s) 779
AvaI CYCGRG 1 cut(s) 214
BaeGI GKGCMC 1 cut(s) 182
BaeI ACNNNNGTAYC 2 cut(s) 406, 439
BalI TGGCCA 1 cut(s) 342
BanI GGYRCC 2 cut(s) 164, 284
BanII GRGCYC 2 cut(s) 182, 612
Bbv12I GWGCWC 1 cut(s) 612
BbvCI CCTCAGC 2 cut(s) 85, 295
BbvI GCAGC 4 cut(s) 12, 556, 702, 760
BccI CCATC 3 cut(s) 255, 677, 875
BciT130I CCWGG 1 cut(s) 555
BcoDI GTCTC 2 cut(s) 789, 907
BfaI CTAG 2 cut(s) 59, 672
BfoI RGCGCY 1 cut(s) 222
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 4 cut(s) 26, 545, 691, 774
BlsI GCNGC 4 cut(s) 27, 546, 692, 775
BmcAI AGTACT 1 cut(s) 818
Bme1390I CCNGG 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 214
BmgBI CACGTC 2 cut(s) 171, 204
BmgT120I GGNCC 3 cut(s) 178, 179, 212
BmiI GGNNCC 4 cut(s) 166, 180, 286, 524
BmrFI CCNGG 1 cut(s) 555
BmrI ACTGGG 1 cut(s) 321
BmuI ACTGGG 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 617, 649
Bpu10I CCTNAGC 2 cut(s) 85, 295
BpuEI CTTGAG 2 cut(s) 17, 927
BsaI GGTCTC 1 cut(s) 907
BsaJI CCNNGG 2 cut(s) 379, 457
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 684
Bse1I ACTGG 2 cut(s) 316, 457
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 379, 457
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 103, 684
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 3 cut(s) 99, 309, 496
BseNI ACTGG 2 cut(s) 316, 457
BseRI GAGGAG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 182
BseXI GCAGC 4 cut(s) 12, 556, 702, 760
BseYI CCCAGC 1 cut(s) 328
BshFI GGCC 5 cut(s) 38, 180, 213, 342, 456
BshNI GGYRCC 2 cut(s) 164, 284
BsiHKAI GWGCWC 1 cut(s) 612
BsiHKCI CYCGRG 1 cut(s) 214
BslFI GGGAC 3 cut(s) 332, 425, 547
BslI CCNNNNNNNGG 2 cut(s) 103, 684
BsmAI GTCTC 2 cut(s) 789, 907
BsmFI GGGAC 3 cut(s) 332, 425, 547
BsmI GAATGC 3 cut(s) 396, 829, 943
BsnI GGCC 5 cut(s) 38, 180, 213, 342, 456
Bso31I GGTCTC 1 cut(s) 907
BsoBI CYCGRG 1 cut(s) 214
Bsp120I GGGCCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 182, 612
BspACI CCGC 6 cut(s) 23, 176, 251, 384, 510, 551
BspANI GGCC 5 cut(s) 38, 180, 213, 342, 456
BspCNI CTCAG 3 cut(s) 98, 308, 495
BspLI GGNNCC 4 cut(s) 166, 180, 286, 524
BspT107I GGYRCC 2 cut(s) 164, 284
BspTNI GGTCTC 1 cut(s) 907
BsrDI GCAATG 1 cut(s) 699
BsrI ACTGG 2 cut(s) 316, 457
BssECI CCNNGG 2 cut(s) 379, 457
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 4 cut(s) 145, 226, 561, 595
Bst6I CTCTTC 1 cut(s) 905
BstC8I GCNNGC 2 cut(s) 178, 211
BstDEI CTNAG 3 cut(s) 85, 295, 482
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 544
BstMAI GTCTC 2 cut(s) 789, 907
BstMWI GCNNNNNNNGC 6 cut(s) 173, 206, 227, 550, 645, 710
BstNI CCWGG 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 553
BstSLI GKGCMC 1 cut(s) 182
BstV1I GCAGC 4 cut(s) 12, 556, 702, 760
BsuRI GGCC 5 cut(s) 38, 180, 213, 342, 456
BtrI CACGTC 2 cut(s) 171, 204
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 5 cut(s) 202, 231, 450, 612, 708
Cac8I GCNNGC 2 cut(s) 178, 211
CfoI GCGC 2 cut(s) 221, 544
Cfr13I GGNCC 3 cut(s) 178, 179, 212
Csp6I GTAC 2 cut(s) 146, 817
CviAII CATG 4 cut(s) 269, 577, 623, 951
CviQI GTAC 2 cut(s) 146, 817
DdeI CTNAG 3 cut(s) 85, 295, 482
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 905
EarI CTCTTC 1 cut(s) 905
Ecl136II GAGCTC 1 cut(s) 610
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 2 cut(s) 182, 612
Eco31I GGTCTC 1 cut(s) 907
Eco53kI GAGCTC 1 cut(s) 610
Eco57I CTGAAG 3 cut(s) 551, 636, 762
Eco88I CYCGRG 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 610
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 379
EcoT38I GRGCYC 2 cut(s) 182, 612
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 4 cut(s) 272, 580, 626, 954
FaqI GGGAC 3 cut(s) 332, 425, 547
FatI CATG 4 cut(s) 268, 576, 622, 950
FauI CCCGC 4 cut(s) 169, 377, 503, 558
Fnu4HI GCNGC 4 cut(s) 26, 545, 691, 774
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 2 cut(s) 182, 612
Fsp4HI GCNGC 4 cut(s) 26, 545, 691, 774
FspBI CTAG 2 cut(s) 59, 672
GlaI GCGC 2 cut(s) 220, 543
GluI GCNGC 4 cut(s) 26, 545, 691, 774
GsaI CCCAGC 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 5 cut(s) 38, 180, 213, 342, 456
HhaI GCGC 2 cut(s) 221, 544
Hin1II CATG 4 cut(s) 272, 580, 626, 954
Hin6I GCGC 2 cut(s) 219, 542
HinP1I GCGC 2 cut(s) 219, 542
HinfI GANTC 3 cut(s) 93, 239, 782
HphI GGTGA 1 cut(s) 779
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 5 cut(s) 461, 485, 570, 679, 781
Hpy8I GTNNAC 1 cut(s) 763
HpyAV CCTTC 4 cut(s) 162, 454, 575, 611
HpyCH4III ACNGT 4 cut(s) 145, 226, 561, 595
HpyCH4IV ACGT 3 cut(s) 170, 203, 666
HpyCH4V TGCA 4 cut(s) 68, 268, 794, 827
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 206, 227, 550, 645, 710
HpyF3I CTNAG 3 cut(s) 85, 295, 482
HpySE526I ACGT 3 cut(s) 170, 203, 666
Hsp92II CATG 4 cut(s) 272, 580, 626, 954
HspAI GCGC 2 cut(s) 219, 542
LmnI GCTCC 2 cut(s) 552, 687
Lsp1109I GCAGC 4 cut(s) 12, 556, 702, 760
MaeI CTAG 2 cut(s) 59, 672
MaeII ACGT 3 cut(s) 170, 203, 666
MaeIII GTNAC 3 cut(s) 307, 319, 946
MboII GAAGA 4 cut(s) 47, 439, 692, 922
MhlI GDGCHC 2 cut(s) 182, 612
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 3 cut(s) 276, 885, 899
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 3 cut(s) 102, 248, 791
Mox20I TGGCCA 1 cut(s) 342
MscI TGGCCA 1 cut(s) 342
MseI TTAA 2 cut(s) 846, 855
MslI CAYNNNNRTG 1 cut(s) 713
Msp20I TGGCCA 1 cut(s) 342
MspA1I CMGCKG 3 cut(s) 25, 176, 332
MspR9I CCNGG 1 cut(s) 555
Mva1269I GAATGC 3 cut(s) 396, 829, 943
MvaI CCWGG 1 cut(s) 555
MwoI GCNNNNNNNGC 6 cut(s) 173, 206, 227, 550, 645, 710
NlaIII CATG 4 cut(s) 272, 580, 626, 954
NlaIV GGNNCC 4 cut(s) 166, 180, 286, 524
NmuCI GTSAC 3 cut(s) 307, 319, 946
PctI GAATGC 3 cut(s) 396, 829, 943
PkrI GCNGC 4 cut(s) 27, 546, 692, 775
PleI GAGTC 3 cut(s) 101, 247, 790
PpsI GAGTC 3 cut(s) 101, 247, 790
PshBI ATTAAT 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 553
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 4 cut(s) 166, 180, 286, 524
PspOMI GGGCCC 1 cut(s) 178
PspPI GGNCC 3 cut(s) 178, 179, 212
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 147, 818
RsaNI GTAC 2 cut(s) 146, 817
RseI CAYNNNNRTG 1 cut(s) 713
SacI GAGCTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 846, 855
SatI GCNGC 4 cut(s) 26, 545, 691, 774
Sau96I GGNCC 3 cut(s) 178, 179, 212
ScaI AGTACT 1 cut(s) 818
SchI GAGTC 3 cut(s) 102, 248, 791
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 2 cut(s) 182, 612
SmiMI CAYNNNNRTG 1 cut(s) 713
SmlI CTYRAG 2 cut(s) 32, 942
SmoI CTYRAG 2 cut(s) 32, 942
Sse9I AATT 3 cut(s) 276, 885, 899
SsiI CCGC 6 cut(s) 23, 176, 251, 384, 510, 551
SspMI CTAG 2 cut(s) 59, 672
SstI GAGCTC 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 553
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 4 cut(s) 145, 226, 561, 595
TaiI ACGT 3 cut(s) 173, 206, 669
TasI AATT 3 cut(s) 276, 885, 899
TatI WGTACW 1 cut(s) 816
Tru1I TTAA 2 cut(s) 846, 855
Tru9I TTAA 2 cut(s) 846, 855
TscAI CASTG 5 cut(s) 202, 231, 457, 619, 715
TseFI GTSAC 3 cut(s) 307, 319, 946
TseI GCWGC 4 cut(s) 25, 544, 690, 773
Tsp45I GTSAC 3 cut(s) 307, 319, 946
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 5 cut(s) 202, 231, 457, 619, 715
VspI ATTAAT 1 cut(s) 855
XagI CCTNNNNNAGG 1 cut(s) 101
XspI CTAG 2 cut(s) 59, 672
ZrmI AGTACT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.