AT1G07730

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003115
Physical Location & Seq
Reverse (-)
2397527 .. 2398696
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G07730.2

Sequence Viewer

Length: 1170 bp
ATGGCAGGCTGCAAAGTACTCTTCTTCCTGATTTTAGCTCTCGCCATTACTTTTGTCTCAGCTGCTCGGCTTCTTGATGAAGAAGAAGATATTGGGTTGGTCCCTTTGCCTACCACGAGTCCTGGTCCTTTACCAACAGTTGGTTTAGGCCCCTTTCCCACTGCTAATTCAGGCCCTGCAACTGGTATCGCTTCAGGTACAGGTTCAGCCTCAGGGGGTTTAGGTTCCTTGGGTACCAATACTGGCCCCGGGCCTCTATCGACCACTGGTTCTAGTCTTTTACCAGTTGCTAGTTCGGGTACCTTGCCTGTTACTGGCCCCGGTCCTCTACCAACCAGTTCTGGTCTCTTACCGGGTGCTAGTTCGGGTAACTTGCCTGGTTCAGGTTCAGGGCCTTTGCCTACTGTTGGTTCAGGTGCTGCAGCTACGGGTCTGGGCGCAGGTGCAGGGTCAGTGATTGGTGGTTCAGTTCCTGACAACACTTTGGTTTTCTTCATGCACGATATACTCGGTGGCTCAAACCCGACAGCGAGGGCTGTGACTGGAGTCGTCGCAAACGCAGCCCTCAGTGGCCAAATTCCATTCGCTAAGCCCAATGGTGCAAACCTCCCTGTTAGTAACGGCGTTCCATCAGACAATAACAACAATGGAATCCTGAACAATAACAACGTCCCACTTCTCGTCGGGCTTGGCGGAACCACTTCCAACATTCTACAGAACAATGGAAACAACATGTTGAACGGTCTTCCCGTGGCTAACGGCGGCCAGCTCCCATCGGGTTCGTCTCTTCAGATGCTCATGTTTGGGACATTGACGGTGATGGACAACGAACTAACCGAAGGACACGAACTCGGGTCTGGTCTGCTGGGTAAAGCCCAAGGCTTCTACGTGGCAAGTGCCCTTGATGGGACCAGCCAGACTATGGCTTTCACGGCTATGTTTGAGAGCGGTGGATATGAAGATAGCATCAGTTTCTTCGGTGTGCATAGAACAGCTGCATCGGAATCTCATCTTGGGGTAATGGGCGGTACTGGTAAGTATGTGAATGCGAGAGGCTTCGCAATTGTCAAGACTTTTACGGGTTCCTCCGGGACCCAGCAGCAGCAGCCGCACCAGTTCACAGATGGGCTCGAGACTGTTCTGGAGTGCACCGTTTATCTTTCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

389

Amino Acids

38.34

Weight (kDa)

4.64

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 259 - 360 6.7e-25 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 431
Acc65I GGTACC 2 cut(s) 233, 299
AccB1I GGYRCC 2 cut(s) 233, 299
AccB7I CCANNNNNTGG 2 cut(s) 140, 922
AccBSI CCGCTC 1 cut(s) 948
AciI CCGC 5 cut(s) 693, 762, 948, 1026, 1109
AcoI YGGCCR 2 cut(s) 571, 763
AcsI RAATTY 1 cut(s) 576
AcuI CTGAAG 2 cut(s) 177, 773
AfaI GTAC 5 cut(s) 18, 199, 235, 301, 1030
AfiI CCNNNNNNNGG 7 cut(s) 140, 182, 314, 383, 407, 906, 922
AflIII ACRYGT 1 cut(s) 732
AgsI TTSAA 1 cut(s) 739
AjnI CCWGG 2 cut(s) 121, 376
AjuI GAANNNNNNNTTGG 4 cut(s) 75, 107, 996, 1028
AluBI AGCT 5 cut(s) 38, 62, 425, 769, 995
AluI AGCT 5 cut(s) 38, 62, 425, 769, 995
Alw21I GWGCWC 1 cut(s) 1151
Alw26I GTCTC 4 cut(s) 61, 350, 789, 1127
Alw44I GTGCAC 1 cut(s) 1147
AlwNI CAGNNNCTG 3 cut(s) 176, 419, 473
Ama87I CYCGRG 3 cut(s) 248, 851, 1130
AoxI GGCC 8 cut(s) 148, 172, 244, 251, 316, 392, 571, 763
ApaLI GTGCAC 1 cut(s) 1147
ApeKI GCWGC 9 cut(s) 9, 62, 419, 422, 560, 995, 1099, 1102, 1105
ApoI RAATTY 1 cut(s) 576
Asp700I GAANNNNTTC 1 cut(s) 700
Asp718I GGTACC 2 cut(s) 233, 299
AspLEI GCGC 1 cut(s) 440
AsuC2I CCSGG 5 cut(s) 249, 250, 321, 354, 1090
AsuHPI GGTGA 1 cut(s) 829
AvaI CYCGRG 3 cut(s) 248, 851, 1130
AvaII GGWCC 5 cut(s) 100, 125, 323, 909, 1092
AxyI CCTNAGG 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 901, 1151
BalI TGGCCA 1 cut(s) 573
BanI GGYRCC 2 cut(s) 233, 299
BanII GRGCYC 1 cut(s) 1131
BauI CACGAG 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 1151
BbvI GCAGC 8 cut(s) 49, 406, 434, 572, 982, 1111, 1114, 1117
BccI CCATC 5 cut(s) 637, 781, 814, 899, 1118
BceAI ACGGC 3 cut(s) 637, 775, 948
BciT130I CCWGG 2 cut(s) 123, 378
BcnI CCSGG 5 cut(s) 249, 250, 321, 354, 1090
BcoDI GTCTC 4 cut(s) 61, 350, 789, 1127
BfaI CTAG 4 cut(s) 273, 291, 360, 1168
BfmI CTRYAG 2 cut(s) 420, 713
BfuAI ACCTGC 1 cut(s) 431
BlpI GCTNAGC 1 cut(s) 588
BmcAI AGTACT 1 cut(s) 18
Bme1390I CCNGG 7 cut(s) 123, 249, 250, 321, 354, 378, 1090
Bme18I GGWCC 5 cut(s) 100, 125, 323, 909, 1092
BmeT110I CYCGRG 3 cut(s) 248, 851, 1130
BmrFI CCNGG 7 cut(s) 123, 249, 250, 321, 354, 378, 1090
BmsI GCATC 3 cut(s) 783, 975, 1007
BoxI GACNNNNGTC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 737
BpmI CTGGAG 2 cut(s) 564, 1163
Bpu1102I GCTNAGC 1 cut(s) 588
BpuMI CCSGG 5 cut(s) 249, 250, 321, 354, 1090
BsaAI YACGTR 1 cut(s) 889
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 6 cut(s) 228, 247, 248, 319, 750, 877
Bsc4I CCNNNNNNNGG 7 cut(s) 140, 182, 314, 383, 407, 906, 922
Bse1I ACTGG 9 cut(s) 187, 247, 271, 284, 319, 336, 547, 1036, 1114
Bse21I CCTNAGG 1 cut(s) 211
BseBI CCWGG 2 cut(s) 123, 378
BseDI CCNNGG 6 cut(s) 228, 247, 248, 319, 750, 877
BseLI CCNNNNNNNGG 7 cut(s) 140, 182, 314, 383, 407, 906, 922
BseMII CTCAG 3 cut(s) 72, 225, 580
BseNI ACTGG 9 cut(s) 187, 247, 271, 284, 319, 336, 547, 1036, 1114
BseSI GKGCMC 2 cut(s) 901, 1151
BseXI GCAGC 8 cut(s) 49, 406, 434, 572, 982, 1111, 1114, 1117
BseYI CCCAGC 2 cut(s) 865, 1095
BsgI GTGCAG 1 cut(s) 465
BshFI GGCC 8 cut(s) 150, 174, 246, 253, 318, 394, 573, 765
BshNI GGYRCC 2 cut(s) 233, 299
BsiHKAI GWGCWC 1 cut(s) 1151
BsiHKCI CYCGRG 3 cut(s) 248, 851, 1130
BsiSI CCGG 4 cut(s) 249, 321, 353, 1089
BslFI GGGAC 5 cut(s) 86, 656, 820, 922, 1105
BslI CCNNNNNNNGG 7 cut(s) 140, 182, 314, 383, 407, 906, 922
BsmAI GTCTC 4 cut(s) 61, 350, 789, 1127
BsmBI CGTCTC 1 cut(s) 789
BsmFI GGGAC 5 cut(s) 86, 656, 820, 922, 1105
BsmI GAATGC 1 cut(s) 1051
BsnI GGCC 8 cut(s) 150, 174, 246, 253, 318, 394, 573, 765
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 3 cut(s) 248, 851, 1130
Bsp1286I GDGCHC 3 cut(s) 901, 1131, 1151
Bsp1720I GCTNAGC 1 cut(s) 588
BspACI CCGC 5 cut(s) 693, 762, 948, 1026, 1109
BspANI GGCC 8 cut(s) 150, 174, 246, 253, 318, 394, 573, 765
BspCNI CTCAG 3 cut(s) 71, 224, 579
BspMAI CTGCAG 1 cut(s) 424
BspMI ACCTGC 1 cut(s) 431
BspT107I GGYRCC 2 cut(s) 233, 299
BspTNI GGTCTC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 948
BsrI ACTGG 9 cut(s) 187, 247, 271, 284, 319, 336, 547, 1036, 1114
BssECI CCNNGG 6 cut(s) 228, 247, 248, 319, 750, 877
BssSI CACGAG 1 cut(s) 115
BssT1I CCWWGG 2 cut(s) 228, 877
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 2 cut(s) 123, 378
Bst4CI ACNGT 6 cut(s) 139, 406, 743, 817, 1138, 1153
Bst6I CTCTTC 2 cut(s) 26, 792
BstBAI YACGTR 1 cut(s) 889
BstC8I GCNNGC 2 cut(s) 7, 767
BstDEI CTNAG 4 cut(s) 58, 211, 566, 588
BstDSI CCRYGG 1 cut(s) 750
BstENI CCTNNNNNAGG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 440
BstMAI GTCTC 4 cut(s) 61, 350, 789, 1127
BstMWI GCNNNNNNNGC 4 cut(s) 560, 932, 1105, 1108
BstNI CCWGG 2 cut(s) 123, 378
BstNSI RCATGY 1 cut(s) 736
BstPAI GACNNNNGTC 1 cut(s) 545
BstSCI CCNGG 7 cut(s) 121, 247, 248, 319, 352, 376, 1088
BstSFI CTRYAG 2 cut(s) 420, 713
BstSLI GKGCMC 2 cut(s) 901, 1151
BstV1I GCAGC 8 cut(s) 49, 406, 434, 572, 982, 1111, 1114, 1117
BstV2I GAAGAC 1 cut(s) 737
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 8 cut(s) 150, 174, 246, 253, 318, 394, 573, 765
BtgI CCRYGG 1 cut(s) 750
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 4 cut(s) 159, 264, 459, 574
BveI ACCTGC 1 cut(s) 431
Cac8I GCNNGC 2 cut(s) 7, 767
CaiI CAGNNNCTG 3 cut(s) 176, 419, 473
CfoI GCGC 1 cut(s) 440
Cfr9I CCCGGG 1 cut(s) 248
Csp6I GTAC 5 cut(s) 17, 198, 234, 300, 1029
CviAII CATG 3 cut(s) 496, 733, 799
CviQI GTAC 5 cut(s) 17, 198, 234, 300, 1029
DdeI CTNAG 4 cut(s) 58, 211, 566, 588
EaeI YGGCCR 2 cut(s) 571, 763
Eam1104I CTCTTC 2 cut(s) 26, 792
EarI CTCTTC 2 cut(s) 26, 792
EciI GGCGGA 1 cut(s) 708
Eco130I CCWWGG 2 cut(s) 228, 877
Eco24I GRGCYC 1 cut(s) 1131
Eco31I GGTCTC 1 cut(s) 350
Eco47I GGWCC 5 cut(s) 100, 125, 323, 909, 1092
Eco57I CTGAAG 2 cut(s) 177, 773
Eco81I CCTNAGG 1 cut(s) 211
Eco88I CYCGRG 3 cut(s) 248, 851, 1130
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoO109I RGGNCCY 4 cut(s) 149, 173, 392, 1092
EcoRII CCWGG 2 cut(s) 121, 376
EcoT14I CCWWGG 2 cut(s) 228, 877
EcoT38I GRGCYC 1 cut(s) 1131
ErhI CCWWGG 2 cut(s) 228, 877
Esp3I CGTCTC 1 cut(s) 789
FaeI CATG 3 cut(s) 499, 736, 802
FaiI YATR 9 cut(s) 497, 506, 734, 800, 923, 938, 957, 987, 1041
FaqI GGGAC 5 cut(s) 86, 656, 820, 922, 1105
FatI CATG 3 cut(s) 495, 732, 798
FriOI GRGCYC 1 cut(s) 1131
FspBI CTAG 4 cut(s) 273, 291, 360, 1168
GlaI GCGC 1 cut(s) 439
GsaI CCCAGC 2 cut(s) 869, 1099
GsuI CTGGAG 2 cut(s) 564, 1163
HaeIII GGCC 8 cut(s) 150, 174, 246, 253, 318, 394, 573, 765
HapII CCGG 4 cut(s) 249, 321, 353, 1089
HhaI GCGC 1 cut(s) 440
Hin1II CATG 3 cut(s) 499, 736, 802
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HinfI GANTC 4 cut(s) 118, 546, 651, 1004
HpaII CCGG 4 cut(s) 249, 321, 353, 1089
HphI GGTGA 1 cut(s) 829
Hpy166II GTNNAC 2 cut(s) 1119, 1149
Hpy188I TCNGA 3 cut(s) 634, 792, 1003
Hpy188III TCNNGA 7 cut(s) 28, 74, 473, 655, 1069, 1132, 1142
Hpy8I GTNNAC 2 cut(s) 1119, 1149
Hpy99I CGWCG 2 cut(s) 554, 686
HpyAV CCTTC 1 cut(s) 833
HpyCH4III ACNGT 6 cut(s) 139, 406, 743, 817, 1138, 1153
HpyCH4IV ACGT 2 cut(s) 669, 888
HpyCH4V TGCA 9 cut(s) 12, 179, 422, 446, 499, 602, 985, 998, 1149
HpyF10VI GCNNNNNNNGC 4 cut(s) 560, 932, 1105, 1108
HpyF3I CTNAG 4 cut(s) 58, 211, 566, 588
HpySE526I ACGT 2 cut(s) 669, 888
Hsp92II CATG 3 cut(s) 499, 736, 802
HspAI GCGC 1 cut(s) 438
KflI GGGWCCC 1 cut(s) 1092
KpnI GGTACC 2 cut(s) 237, 303
LmnI GCTCC 1 cut(s) 774
Lsp1109I GCAGC 8 cut(s) 49, 406, 434, 572, 982, 1111, 1114, 1117
LweI GCATC 3 cut(s) 783, 975, 1007
MaeI CTAG 4 cut(s) 273, 291, 360, 1168
MaeII ACGT 2 cut(s) 669, 888
MaeIII GTNAC 4 cut(s) 310, 368, 538, 617
MbiI CCGCTC 1 cut(s) 948
MfeI CAATTG 1 cut(s) 1062
MhlI GDGCHC 3 cut(s) 901, 1131, 1151
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 3 cut(s) 166, 576, 1062
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 2 cut(s) 127, 555
MmeI TCCRAC 1 cut(s) 729
MnlI CCTC 8 cut(s) 220, 264, 336, 525, 575, 617, 1046, 1096
Mox20I TGGCCA 1 cut(s) 573
MroXI GAANNNNTTC 1 cut(s) 700
MscI TGGCCA 1 cut(s) 573
MslI CAYNNNNRTG 1 cut(s) 935
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 2 cut(s) 62, 995
MspI CCGG 4 cut(s) 249, 321, 353, 1089
MspR9I CCNGG 7 cut(s) 123, 249, 250, 321, 354, 378, 1090
MunI CAATTG 1 cut(s) 1062
Mva1269I GAATGC 1 cut(s) 1051
MvaI CCWGG 2 cut(s) 123, 378
MwoI GCNNNNNNNGC 4 cut(s) 560, 932, 1105, 1108
NciI CCSGG 5 cut(s) 249, 250, 321, 354, 1090
NlaIII CATG 3 cut(s) 499, 736, 802
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 1 cut(s) 736
PaeR7I CTCGAG 1 cut(s) 1130
PaqCI CACCTGC 1 cut(s) 431
PciI ACATGT 1 cut(s) 732
PcsI WCGNNNNNNNCGW 2 cut(s) 747, 834
PctI GAATGC 1 cut(s) 1051
PdmI GAANNNNTTC 1 cut(s) 700
PfeI GAWTC 2 cut(s) 651, 1004
PflMI CCANNNNNTGG 2 cut(s) 140, 922
PfoI TCCNGGA 1 cut(s) 1088
PleI GAGTC 2 cut(s) 126, 554
PpsI GAGTC 2 cut(s) 126, 554
Ppu21I YACGTR 1 cut(s) 889
PpuMI RGGWCCY 1 cut(s) 1092
PscI ACATGT 1 cut(s) 732
PshAI GACNNNNGTC 1 cut(s) 545
Psp5II RGGWCCY 1 cut(s) 1092
Psp6I CCWGG 2 cut(s) 121, 376
PspFI CCCAGC 2 cut(s) 865, 1095
PspGI CCWGG 2 cut(s) 121, 376
PspPPI RGGWCCY 1 cut(s) 1092
PstI CTGCAG 1 cut(s) 424
PstNI CAGNNNCTG 3 cut(s) 176, 419, 473
PvuII CAGCTG 2 cut(s) 62, 995
RsaI GTAC 5 cut(s) 18, 199, 235, 301, 1030
RsaNI GTAC 5 cut(s) 17, 198, 234, 300, 1029
RseI CAYNNNNRTG 1 cut(s) 935
ScaI AGTACT 1 cut(s) 18
SchI GAGTC 2 cut(s) 127, 555
ScrFI CCNGG 7 cut(s) 123, 249, 250, 321, 354, 378, 1090
SduI GDGCHC 3 cut(s) 901, 1131, 1151
SfaNI GCATC 3 cut(s) 783, 975, 1007
SfcI CTRYAG 2 cut(s) 420, 713
Sfr274I CTCGAG 1 cut(s) 1130
SinI GGWCC 5 cut(s) 100, 125, 323, 909, 1092
SlaI CTCGAG 1 cut(s) 1130
SmaI CCCGGG 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 1130
SmoI CTYRAG 1 cut(s) 1130
Sse9I AATT 3 cut(s) 166, 576, 1062
SsiI CCGC 5 cut(s) 693, 762, 948, 1026, 1109
SspMI CTAG 4 cut(s) 273, 291, 360, 1168
StyD4I CCNGG 7 cut(s) 121, 247, 248, 319, 352, 376, 1088
StyI CCWWGG 2 cut(s) 228, 877
TaaI ACNGT 6 cut(s) 139, 406, 743, 817, 1138, 1153
TaiI ACGT 2 cut(s) 672, 891
TaqI TCGA 2 cut(s) 260, 1131
TasI AATT 3 cut(s) 166, 576, 1062
TatI WGTACW 1 cut(s) 16
TauI GCSGC 2 cut(s) 765, 1111
TfiI GAWTC 2 cut(s) 651, 1004
TscAI CASTG 4 cut(s) 166, 271, 459, 574
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 9 cut(s) 9, 62, 419, 422, 560, 995, 1099, 1102, 1105
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 3 cut(s) 93, 484, 972
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 4 cut(s) 166, 271, 459, 574
Van91I CCANNNNNTGG 2 cut(s) 140, 922
VneI GTGCAC 1 cut(s) 1147
VpaK11BI GGWCC 5 cut(s) 100, 125, 323, 909, 1092
XagI CCTNNNNNAGG 1 cut(s) 381
XapI RAATTY 1 cut(s) 576
XceI RCATGY 1 cut(s) 736
XcmI CCANNNNNNNNNTGG 2 cut(s) 919, 1121
XhoI CTCGAG 1 cut(s) 1130
XmaI CCCGGG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 700
XspI CTAG 4 cut(s) 273, 291, 360, 1168
ZrmI AGTACT 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.