AT2G28670

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003115
Physical Location & Seq
Reverse (-)
12300265 .. 12302358
2094 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28670.2

Sequence Viewer

Length: 1311 bp
ATGGCTGGTCAAAAGATACTTTCTTTACTGGTTATAGCTCTTGTTGTTACTTTTGCAGCTGCGGCTCGGCTTCTTGATGAAGAAAATGCATTCTCAGCTACCACAACCACCCTTGGCTCAGGTTCAGGTTCCACCGGAATTGGTTTTGGTGCAGGAACAGGGTCAAGTGGTTCAGGTTCCACGGGATTCGGTTTTGGTGCAGGATCAGGGTCATCTGGTTCAGGTTCTACTGGAAGCGGTCTCGGTGCAGGTACAGGGTCAATACCATCAAGTGGTTCAGGTCCTGGTCTTCTACCAACTGCCAGTTCTGTTCCTGGTTCTTTAGCGGGTGGTGGTTCAGGTTCCTTGCCTACCACTGGTTCAGCCACTGGTGCAGGTGCAGGTACCGGTTCAGCTCTTGGAGGTGGTCCAGGTGCTGGCTCAGCTCTTGGTGGTGGTGCGGGTGCCGGTCCAGCTCTTGGTGGTGGTGCGGGTGCCGGTCCAGCTCTTGGTGGTGGTGCAGGTGCTGGTTCAGCTCTTGGTGGTGGTGGTGCAGGTGCTGGTCCAGCTCTTGGTGGTGGTGTTGCAGGTTCTGGTTCAGCTCTTGGTGGTGGTGCAAGTGCTGGTCCTGACAATACGTTGGTATTTTTTATGCATGATATACTCGGTGGTTCAAATCCCACTGCCAGAGCCGTAACTGGAGTCGTCGCAAATCCGGCTTTAAGCGGGCAACTTCCATTTGCTAAACCCAACGGCGCAAACCTTCCTGTAAGCAACGGAGTTCCGTCAAACAACAACAACAATGGGATTGTCAACAACAACAATGTCCCTTTCCTCGTTGGACTCGGCGGAACCACAGCTAACATTCTCCAGAACAACAATAACGGAAACAACATTTTAAATGGTTTCCCTGTTGCTAGTGGCGGTCAGCTCCCGAGCGGTTCTGCTCTTCAGATGCTAATGTTCGGGACAATGACAGTAATCGATGATGAACTAACTGAAGGCCATGAGCTCGGATCTGGCTTACTCGGGAAAGCGCAGGGATATTATGTGGCGAGTGCAATTGACGGAACTAGCCAGACAATGGCTTTCACCGCGATGTTTGAAAGCGGCGGTTATGAAGATAGTATAAGCTTCTTTGGGGTTCTCAGAACAGCGGTATCAGAATCGCATATAGGAGTAATGGGAGGTACCGGAAAGTATGTGAATGCGAGGGGATTCGCGATTCTGAAGACGTTTACAGGATCTAGCGGGACGCAGCAGAATCAGCCGCATCAGTTCACTGATGGGCTTGAGACTGTTGTTGAGTGTACCGTTTATCTTTCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

436

Amino Acids

40.74

Weight (kDa)

4.69

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 97 - 198 5.6e-24 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 365, 491, 524
Acc36I ACCTGC 6 cut(s) 239, 365, 371, 491, 524, 557
Acc65I GGTACC 2 cut(s) 383, 1169
AccB1I GGYRCC 4 cut(s) 383, 443, 473, 1169
AccB7I CCANNNNNTGG 6 cut(s) 272, 416, 458, 488, 551, 1063
AccBSI CCGCTC 1 cut(s) 918
AccII CGCG 2 cut(s) 1076, 1202
AclWI GGATC 3 cut(s) 211, 1003, 1231
AcuI CTGAAG 3 cut(s) 914, 999, 1229
AfaI GTAC 4 cut(s) 253, 385, 1171, 1291
AfiI CCNNNNNNNGG 7 cut(s) 272, 356, 416, 458, 488, 551, 1063
AgeI ACCGGT 1 cut(s) 386
AgsI TTSAA 2 cut(s) 654, 1085
AjnI CCWGG 3 cut(s) 283, 313, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 289, 321
Alw21I GWGCWC 1 cut(s) 993
Alw26I GTCTC 2 cut(s) 245, 1268
AlwI GGATC 3 cut(s) 211, 1003, 1231
AlwNI CAGNNNCTG 6 cut(s) 284, 368, 416, 506, 539, 572
Ama87I CYCGRG 2 cut(s) 913, 1007
AoxI GGCC 1 cut(s) 982
ApeKI GCWGC 3 cut(s) 56, 59, 1237
AsiGI ACCGGT 1 cut(s) 386
Asp718I GGTACC 2 cut(s) 383, 1169
AspLEI GCGC 2 cut(s) 737, 1018
AspS9I GGNCC 6 cut(s) 281, 407, 449, 479, 542, 605
AsuHPI GGTGA 1 cut(s) 1063
AvaI CYCGRG 2 cut(s) 913, 1007
AvaII GGWCC 6 cut(s) 281, 407, 449, 479, 542, 605
BaeI ACNNNNGTAYC 2 cut(s) 1122, 1155
BanI GGYRCC 4 cut(s) 383, 443, 473, 1169
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 2 cut(s) 281, 1217
Bbv12I GWGCWC 1 cut(s) 993
BbvI GCAGC 3 cut(s) 46, 68, 1249
BccI CCATC 2 cut(s) 274, 1259
BceAI ACGGC 2 cut(s) 656, 748
BciT130I CCWGG 3 cut(s) 285, 315, 411
BcoDI GTCTC 2 cut(s) 245, 1268
BfaI CTAG 4 cut(s) 897, 1053, 1227, 1309
BfuAI ACCTGC 6 cut(s) 239, 365, 371, 491, 524, 557
BisI GCNGC 6 cut(s) 57, 60, 63, 1090, 1238, 1250
BlpI GCTNAGC 1 cut(s) 421
BlsI GCNGC 6 cut(s) 58, 61, 64, 1091, 1239, 1251
Bme1390I CCNGG 3 cut(s) 285, 315, 411
Bme18I GGWCC 6 cut(s) 281, 407, 449, 479, 542, 605
BmeT110I CYCGRG 2 cut(s) 913, 1007
BmgT120I GGNCC 6 cut(s) 281, 407, 449, 479, 542, 605
BmiI GGNNCC 8 cut(s) 130, 178, 343, 385, 445, 475, 832, 1171
BmrFI CCNGG 3 cut(s) 285, 315, 411
BmsI GCATC 2 cut(s) 924, 1261
BpiI GAAGAC 2 cut(s) 281, 1217
BpmI CTGGAG 2 cut(s) 699, 833
Bpu10I CCTNAGC 1 cut(s) 118
Bpu1102I GCTNAGC 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 1292
Bsa29I ATCGAT 1 cut(s) 963
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 112, 180
BsaWI WCCGGW 3 cut(s) 134, 386, 1172
Bsc4I CCNNNNNNNGG 7 cut(s) 272, 356, 416, 458, 488, 551, 1063
Bse118I RCCGGY 3 cut(s) 386, 446, 476
Bse1I ACTGG 6 cut(s) 33, 235, 303, 361, 373, 682
BseBI CCWGG 3 cut(s) 285, 315, 411
BseCI ATCGAT 1 cut(s) 963
BseDI CCNNGG 2 cut(s) 112, 180
BseLI CCNNNNNNNGG 7 cut(s) 272, 356, 416, 458, 488, 551, 1063
BseMII CTCAG 4 cut(s) 108, 132, 435, 1141
BseNI ACTGG 6 cut(s) 33, 235, 303, 361, 373, 682
BseXI GCAGC 3 cut(s) 46, 68, 1249
BsgI GTGCAG 7 cut(s) 171, 219, 267, 393, 399, 519, 552
Bsh1236I CGCG 2 cut(s) 1076, 1202
BshFI GGCC 1 cut(s) 984
BshNI GGYRCC 4 cut(s) 383, 443, 473, 1169
BshTI ACCGGT 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 963
BsiHKAI GWGCWC 1 cut(s) 993
BsiHKCI CYCGRG 2 cut(s) 913, 1007
BsiSI CCGG 6 cut(s) 135, 387, 447, 477, 695, 1173
BslFI GGGAC 3 cut(s) 791, 961, 1246
BslI CCNNNNNNNGG 7 cut(s) 272, 356, 416, 458, 488, 551, 1063
BsmAI GTCTC 2 cut(s) 245, 1268
BsmFI GGGAC 3 cut(s) 791, 961, 1246
BsmI GAATGC 2 cut(s) 89, 1192
BsnI GGCC 1 cut(s) 984
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 2 cut(s) 913, 1007
Bsp1286I GDGCHC 1 cut(s) 993
Bsp143I GATC 3 cut(s) 203, 995, 1223
Bsp1720I GCTNAGC 1 cut(s) 421
Bsp68I TCGCGA 1 cut(s) 1202
BspANI GGCC 1 cut(s) 984
BspCNI CTCAG 4 cut(s) 107, 131, 434, 1140
BspDI ATCGAT 1 cut(s) 963
BspFNI CGCG 2 cut(s) 1076, 1202
BspLI GGNNCC 8 cut(s) 130, 178, 343, 385, 445, 475, 832, 1171
BspMI ACCTGC 6 cut(s) 239, 365, 371, 491, 524, 557
BspPI GGATC 3 cut(s) 211, 1003, 1231
BspQI GCTCTTC 1 cut(s) 933
BspT107I GGYRCC 4 cut(s) 383, 443, 473, 1169
BspTNI GGTCTC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 918
BsrFI RCCGGY 3 cut(s) 386, 446, 476
BsrI ACTGG 6 cut(s) 33, 235, 303, 361, 373, 682
BssAI RCCGGY 3 cut(s) 386, 446, 476
BssECI CCNNGG 2 cut(s) 112, 180
BssMI GATC 3 cut(s) 203, 995, 1223
BssT1I CCWWGG 1 cut(s) 112
Bst2UI CCWGG 3 cut(s) 285, 315, 411
Bst4CI ACNGT 3 cut(s) 958, 1279, 1294
Bst6I CTCTTC 1 cut(s) 933
BstC8I GCNNGC 2 cut(s) 418, 707
BstDEI CTNAG 4 cut(s) 94, 118, 421, 1127
BstDSI CCRYGG 1 cut(s) 180
BstFNI CGCG 2 cut(s) 1076, 1202
BstHHI GCGC 2 cut(s) 737, 1018
BstKTI GATC 3 cut(s) 206, 998, 1226
BstMAI GTCTC 2 cut(s) 245, 1268
BstMBI GATC 3 cut(s) 203, 995, 1223
BstNI CCWGG 3 cut(s) 285, 315, 411
BstSCI CCNGG 3 cut(s) 283, 313, 409
BstUI CGCG 2 cut(s) 1076, 1202
BstV1I GCAGC 3 cut(s) 46, 68, 1249
BstV2I GAAGAC 2 cut(s) 281, 1217
BstX2I RGATCY 2 cut(s) 995, 1223
BstYI RGATCY 2 cut(s) 995, 1223
Bsu15I ATCGAT 1 cut(s) 963
BsuRI GGCC 1 cut(s) 984
BsuTUI ATCGAT 1 cut(s) 963
BtgI CCRYGG 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 1091
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 4 cut(s) 354, 366, 660, 1260
BtuMI TCGCGA 1 cut(s) 1202
BveI ACCTGC 6 cut(s) 239, 365, 371, 491, 524, 557
Cac8I GCNNGC 2 cut(s) 418, 707
CaiI CAGNNNCTG 6 cut(s) 284, 368, 416, 506, 539, 572
CfoI GCGC 2 cut(s) 737, 1018
Cfr10I RCCGGY 3 cut(s) 386, 446, 476
Cfr13I GGNCC 6 cut(s) 281, 407, 449, 479, 542, 605
ClaI ATCGAT 1 cut(s) 963
CseI GACGC 1 cut(s) 1243
Csp6I GTAC 4 cut(s) 252, 384, 1170, 1290
CspAI ACCGGT 1 cut(s) 386
CspCI CAANNNNNGTGG 2 cut(s) 121, 156
CviAII CATG 2 cut(s) 635, 986
CviQI GTAC 4 cut(s) 252, 384, 1170, 1290
DdeI CTNAG 4 cut(s) 94, 118, 421, 1127
DpnI GATC 3 cut(s) 205, 997, 1225
DpnII GATC 3 cut(s) 203, 995, 1223
DraI TTTAAA 1 cut(s) 879
Eam1104I CTCTTC 1 cut(s) 933
EarI CTCTTC 1 cut(s) 933
EciI GGCGGA 1 cut(s) 843
Ecl136II GAGCTC 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 993
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 6 cut(s) 281, 407, 449, 479, 542, 605
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 3 cut(s) 914, 999, 1229
Eco88I CYCGRG 2 cut(s) 913, 1007
EcoICRI GAGCTC 1 cut(s) 991
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 3 cut(s) 283, 313, 409
EcoT14I CCWWGG 1 cut(s) 112
EcoT22I ATGCAT 2 cut(s) 91, 636
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 2 cut(s) 638, 989
FaqI GGGAC 3 cut(s) 791, 961, 1246
FatI CATG 2 cut(s) 634, 985
FauI CCCGC 5 cut(s) 319, 433, 463, 698, 1223
Fnu4HI GCNGC 6 cut(s) 57, 60, 63, 1090, 1238, 1250
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 6 cut(s) 57, 60, 63, 1090, 1238, 1250
FspBI CTAG 4 cut(s) 897, 1053, 1227, 1309
GlaI GCGC 2 cut(s) 736, 1017
GluI GCNGC 6 cut(s) 57, 60, 63, 1090, 1238, 1250
GsuI CTGGAG 2 cut(s) 699, 833
HaeIII GGCC 1 cut(s) 984
HapII CCGG 6 cut(s) 135, 387, 447, 477, 695, 1173
HgaI GACGC 1 cut(s) 1243
HhaI GCGC 2 cut(s) 737, 1018
Hin1II CATG 2 cut(s) 638, 989
Hin6I GCGC 2 cut(s) 735, 1016
HinP1I GCGC 2 cut(s) 735, 1016
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 1 cut(s) 1111
HinfI GANTC 7 cut(s) 186, 681, 822, 1145, 1197, 1204, 1243
HpaII CCGG 6 cut(s) 135, 387, 447, 477, 695, 1173
HphI GGTGA 1 cut(s) 1063
Hpy166II GTNNAC 4 cut(s) 793, 1218, 1260, 1290
Hpy188I TCNGA 5 cut(s) 933, 995, 1130, 1144, 1209
Hpy188III TCNNGA 7 cut(s) 74, 608, 850, 913, 946, 1009, 1201
Hpy8I GTNNAC 4 cut(s) 793, 1218, 1260, 1290
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 2 cut(s) 752, 974
HpyCH4III ACNGT 3 cut(s) 958, 1279, 1294
HpyCH4IV ACGT 2 cut(s) 617, 1214
HpyF3I CTNAG 4 cut(s) 94, 118, 421, 1127
HpySE526I ACGT 2 cut(s) 617, 1214
Hsp92II CATG 2 cut(s) 638, 989
HspAI GCGC 2 cut(s) 735, 1016
KpnI GGTACC 2 cut(s) 387, 1173
Kzo9I GATC 3 cut(s) 203, 995, 1223
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 1 cut(s) 915
Lsp1109I GCAGC 3 cut(s) 46, 68, 1249
LweI GCATC 2 cut(s) 924, 1261
MaeI CTAG 4 cut(s) 897, 1053, 1227, 1309
MaeII ACGT 2 cut(s) 617, 1214
MaeIII GTNAC 2 cut(s) 46, 673
MalI GATC 3 cut(s) 205, 997, 1225
MbiI CCGCTC 1 cut(s) 918
MboI GATC 3 cut(s) 203, 995, 1223
MboII GAAGA 5 cut(s) 92, 281, 920, 1112, 1222
MfeI CAATTG 1 cut(s) 1041
MflI RGATCY 2 cut(s) 995, 1223
MhlI GDGCHC 1 cut(s) 993
MluCI AATT 2 cut(s) 138, 1041
MlyI GAGTC 2 cut(s) 690, 816
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 4 cut(s) 395, 824, 1160, 1185
Mph1103I ATGCAT 2 cut(s) 91, 636
MseI TTAA 2 cut(s) 701, 878
MslI CAYNNNNRTG 1 cut(s) 1076
MspA1I CMGCKG 2 cut(s) 59, 1136
MspI CCGG 6 cut(s) 135, 387, 447, 477, 695, 1173
MspR9I CCNGG 3 cut(s) 285, 315, 411
MunI CAATTG 1 cut(s) 1041
Mva1269I GAATGC 2 cut(s) 89, 1192
MvaI CCWGG 3 cut(s) 285, 315, 411
MvnI CGCG 2 cut(s) 1076, 1202
NdeII GATC 3 cut(s) 203, 995, 1223
NlaIII CATG 2 cut(s) 638, 989
NlaIV GGNNCC 8 cut(s) 130, 178, 343, 385, 445, 475, 832, 1171
NmeAIII GCCGAG 2 cut(s) 46, 804
NruI TCGCGA 1 cut(s) 1202
NsiI ATGCAT 2 cut(s) 91, 636
PaqCI CACCTGC 3 cut(s) 365, 491, 524
PciSI GCTCTTC 1 cut(s) 933
PctI GAATGC 2 cut(s) 89, 1192
PfeI GAWTC 5 cut(s) 186, 1145, 1197, 1204, 1243
PflMI CCANNNNNTGG 6 cut(s) 272, 416, 458, 488, 551, 1063
PinAI ACCGGT 1 cut(s) 386
PkrI GCNGC 6 cut(s) 58, 61, 64, 1091, 1239, 1251
PleI GAGTC 2 cut(s) 689, 816
PpsI GAGTC 2 cut(s) 689, 816
PpuMI RGGWCCY 1 cut(s) 281
Psp124BI GAGCTC 1 cut(s) 993
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 3 cut(s) 283, 313, 409
PspGI CCWGG 3 cut(s) 283, 313, 409
PspN4I GGNNCC 8 cut(s) 130, 178, 343, 385, 445, 475, 832, 1171
PspPI GGNCC 6 cut(s) 281, 407, 449, 479, 542, 605
PspPPI RGGWCCY 1 cut(s) 281
PstNI CAGNNNCTG 6 cut(s) 284, 368, 416, 506, 539, 572
PsuI RGATCY 2 cut(s) 995, 1223
PvuII CAGCTG 1 cut(s) 59
RruI TCGCGA 1 cut(s) 1202
RsaI GTAC 4 cut(s) 253, 385, 1171, 1291
RsaNI GTAC 4 cut(s) 252, 384, 1170, 1290
RseI CAYNNNNRTG 1 cut(s) 1076
SacI GAGCTC 1 cut(s) 993
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 2 cut(s) 701, 878
SatI GCNGC 6 cut(s) 57, 60, 63, 1090, 1238, 1250
Sau3AI GATC 3 cut(s) 203, 995, 1223
Sau96I GGNCC 6 cut(s) 281, 407, 449, 479, 542, 605
SchI GAGTC 2 cut(s) 690, 816
ScrFI CCNGG 3 cut(s) 285, 315, 411
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 2 cut(s) 924, 1261
SinI GGWCC 6 cut(s) 281, 407, 449, 479, 542, 605
SmiMI CAYNNNNRTG 1 cut(s) 1076
SmlI CTYRAG 1 cut(s) 1271
SmoI CTYRAG 1 cut(s) 1271
Sse9I AATT 2 cut(s) 138, 1041
SspMI CTAG 4 cut(s) 897, 1053, 1227, 1309
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 3 cut(s) 283, 313, 409
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 3 cut(s) 958, 1279, 1294
TaiI ACGT 2 cut(s) 620, 1217
TaqI TCGA 1 cut(s) 963
TasI AATT 2 cut(s) 138, 1041
TauI GCSGC 3 cut(s) 65, 1092, 1252
TfiI GAWTC 5 cut(s) 186, 1145, 1197, 1204, 1243
Tru1I TTAA 2 cut(s) 701, 878
Tru9I TTAA 2 cut(s) 701, 878
TscAI CASTG 4 cut(s) 361, 373, 667, 1267
TseI GCWGC 3 cut(s) 56, 59, 1237
TspDTI ATGAA 3 cut(s) 93, 984, 1113
TspGWI ACGGA 4 cut(s) 753, 771, 879, 1062
TspRI CASTG 4 cut(s) 361, 373, 667, 1267
Van91I CCANNNNNTGG 6 cut(s) 272, 416, 458, 488, 551, 1063
VpaK11BI GGWCC 6 cut(s) 281, 407, 449, 479, 542, 605
XspI CTAG 4 cut(s) 897, 1053, 1227, 1309
Zsp2I ATGCAT 2 cut(s) 91, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.