MD07G1189300.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003115
Physical Location & Seq
Reverse (-)
26961760 .. 26962665
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1189300.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGAAGCTCACAAAATTTTCTCCATCCCTGCCAAGGCCACTGCCTACCTCCTATTTCTTACTCTCACCATTTTCTCTGCTGCTTCAGCACGAAATCTCGAGGAGCAGCCACAAGTTCCGGTAGTTCCCCCAGTGGTGTCTGACATGTTTGATACACCTGTCACAAATGCCGCACTAGCTACACCAGCAGCAGCTACTAGTACTGCAACAGTCGCAAATGCTGCTGACCACCCTGCCTTGATCTTCTTTATGCACGACATCCTCGGAGGATCAAACCCCACTGCCCGAGCCATGACAGGGATAGTCAACAACCCTGCAGCCAACGGTCAAGTCCCATTTGCCAAGCCCAAAGGGGCTGTTCTACCATTCAACAACGGAGTACCCCAGAACAACAACAACAACGGTCTCATAAACAACAATAACATCCCATTTCTCACAGGGCTTAGTGGAACAACACCAAACGTTGGACAAAACAACAACAGAAATGGTGGCGGGTTTGGAAACTTTCCGATAAATGGTGGGCAGCTCCTGCCAGGCTCCTCCGCGCTCCAAAATCTCATGTTTGGAACAATGAGCGTGTTTGATGATGAGTTCACTGAAGGGCATGAGCTTAGCTCTGGCTTGGTTGGCAAGGCACAAGGTTTTTATGTAGTGAGCTCTGAGGACGGTACTAGTCAGACTATGGCTTTCACTGCTATGTTCCAAAGTGGGAGTTACACCGACAGTCTTAGCTTCTTTGGGGTTCACCGTACAGCTGTGTCAGAGTCTCATTTGGCCATTATGGGTGGCACCGGAAAGTATGTCAATGCTAAGGGTTTCGCGTTGGTTAAGACCTTCCTGGCTTCCAATCAGCAAACTGATGGGGCGGAGACACTGTTGGAGTTCACCGTTTATGTCACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

302

Amino Acids

31.5

Weight (kDa)

5.24

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 182 - 299 1.6e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 788
AccB7I CCANNNNNTGG 1 cut(s) 464
AccII CGCG 2 cut(s) 545, 821
AciI CCGC 4 cut(s) 171, 492, 543, 866
AclI AACGTT 1 cut(s) 462
AclWI GGATC 1 cut(s) 277
AcoI YGGCCR 1 cut(s) 774
AcsI RAATTY 1 cut(s) 15
AcuI CTGAAG 2 cut(s) 69, 618
AfaI GTAC 4 cut(s) 202, 381, 670, 751
AfiI CCNNNNNNNGG 4 cut(s) 34, 297, 464, 515
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 1 cut(s) 370
AhlI ACTAGT 2 cut(s) 197, 671
AjnI CCWGG 2 cut(s) 532, 837
AluBI AGCT 9 cut(s) 8, 179, 194, 526, 610, 615, 657, 732, 755
AluI AGCT 9 cut(s) 8, 179, 194, 526, 610, 615, 657, 732, 755
Alw21I GWGCWC 1 cut(s) 659
Alw26I GTCTC 3 cut(s) 410, 771, 863
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 529
Ama87I CYCGRG 2 cut(s) 98, 285
AoxI GGCC 2 cut(s) 36, 774
ApeKI GCWGC 7 cut(s) 80, 106, 188, 191, 221, 317, 523
ApoI RAATTY 1 cut(s) 15
AspLEI GCGC 1 cut(s) 547
AsuHPI GGTGA 3 cut(s) 58, 737, 877
AvaI CYCGRG 2 cut(s) 98, 285
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BalI TGGCCA 1 cut(s) 776
BanI GGYRCC 1 cut(s) 788
BanII GRGCYC 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 659
BbvI GCAGC 7 cut(s) 67, 118, 200, 203, 208, 329, 535
BccI CCATC 2 cut(s) 32, 854
BciT130I CCWGG 2 cut(s) 534, 839
BcoDI GTCTC 3 cut(s) 410, 771, 863
BcuI ACTAGT 2 cut(s) 197, 671
BfaI CTAG 3 cut(s) 176, 198, 672
BfmI CTRYAG 1 cut(s) 315
BisI GCNGC 8 cut(s) 81, 107, 171, 189, 192, 222, 318, 524
BlpI GCTNAGC 1 cut(s) 611
BlsI GCNGC 8 cut(s) 82, 108, 172, 190, 193, 223, 319, 525
BmcAI AGTACT 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 534, 839
BmeT110I CYCGRG 2 cut(s) 98, 285
BmiI GGNNCC 2 cut(s) 538, 790
BmrFI CCNGG 2 cut(s) 534, 839
BmrI ACTGGG 1 cut(s) 125
BmuI ACTGGG 1 cut(s) 125
BplI GAGNNNNNCTC 2 cut(s) 599, 631
Bpu10I CCTNAGC 1 cut(s) 810
Bpu1102I GCTNAGC 1 cut(s) 611
BsaI GGTCTC 1 cut(s) 410
BsaJI CCNNGG 2 cut(s) 33, 262
BsaWI WCCGGW 2 cut(s) 118, 791
BsaXI ACNNNNNCTCC 2 cut(s) 860, 890
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 297, 464, 515
Bse1I ACTGG 1 cut(s) 131
BseBI CCWGG 2 cut(s) 534, 839
BseDI CCNNGG 2 cut(s) 33, 262
BseGI GGATG 3 cut(s) 24, 258, 423
BseLI CCNNNNNNNGG 4 cut(s) 34, 297, 464, 515
BseMII CTCAG 1 cut(s) 651
BseNI ACTGG 1 cut(s) 131
BseRI GAGGAG 2 cut(s) 116, 529
BseXI GCAGC 7 cut(s) 67, 118, 200, 203, 208, 329, 535
Bsh1236I CGCG 2 cut(s) 545, 821
BshFI GGCC 2 cut(s) 38, 776
BshNI GGYRCC 1 cut(s) 788
BsiHKAI GWGCWC 1 cut(s) 659
BsiHKCI CYCGRG 2 cut(s) 98, 285
BsiSI CCGG 2 cut(s) 119, 792
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 4 cut(s) 34, 297, 464, 515
BsmAI GTCTC 3 cut(s) 410, 771, 863
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 2 cut(s) 38, 776
Bso31I GGTCTC 1 cut(s) 410
BsoBI CYCGRG 2 cut(s) 98, 285
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 2 cut(s) 240, 269
Bsp1720I GCTNAGC 1 cut(s) 611
BspACI CCGC 4 cut(s) 171, 492, 543, 866
BspANI GGCC 2 cut(s) 38, 776
BspCNI CTCAG 1 cut(s) 652
BspFNI CGCG 2 cut(s) 545, 821
BspLI GGNNCC 2 cut(s) 538, 790
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 1 cut(s) 277
BspT107I GGYRCC 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 410
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 2 cut(s) 33, 262
BssMI GATC 2 cut(s) 240, 269
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 534, 839
Bst4CI ACNGT 8 cut(s) 211, 326, 404, 668, 725, 749, 876, 889
BstAPI GCANNNNNTGC 2 cut(s) 221, 529
BstDEI CTNAG 5 cut(s) 443, 611, 660, 728, 810
BstF5I GGATG 3 cut(s) 24, 258, 423
BstFNI CGCG 2 cut(s) 545, 821
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 2 cut(s) 243, 272
BstMAI GTCTC 3 cut(s) 410, 771, 863
BstMBI GATC 2 cut(s) 240, 269
BstMWI GCNNNNNNNGC 8 cut(s) 86, 176, 185, 212, 221, 529, 627, 692
BstNI CCWGG 2 cut(s) 534, 839
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 2 cut(s) 532, 837
BstSFI CTRYAG 1 cut(s) 315
BstUI CGCG 2 cut(s) 545, 821
BstV1I GCAGC 7 cut(s) 67, 118, 200, 203, 208, 329, 535
BsuRI GGCC 2 cut(s) 38, 776
BtsCI GGATG 3 cut(s) 24, 258, 423
BtsI GCAGTG 3 cut(s) 39, 279, 690
BtsIMutI CAGTG 6 cut(s) 39, 138, 279, 594, 690, 872
CaiI CAGNNNCTG 1 cut(s) 529
CfoI GCGC 1 cut(s) 547
Csp6I GTAC 4 cut(s) 201, 380, 669, 750
CviAII CATG 4 cut(s) 145, 292, 559, 605
CviQI GTAC 4 cut(s) 201, 380, 669, 750
DdeI CTNAG 5 cut(s) 443, 611, 660, 728, 810
DpnI GATC 2 cut(s) 242, 271
DpnII GATC 2 cut(s) 240, 269
EaeI YGGCCR 1 cut(s) 774
EciI GGCGGA 1 cut(s) 881
Ecl136II GAGCTC 1 cut(s) 657
Eco130I CCWWGG 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 657
Eco57I CTGAAG 2 cut(s) 69, 618
Eco88I CYCGRG 2 cut(s) 98, 285
EcoICRI GAGCTC 1 cut(s) 657
EcoRII CCWGG 2 cut(s) 532, 837
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 4 cut(s) 148, 295, 562, 608
FaqI GGGAC 1 cut(s) 317
FatI CATG 4 cut(s) 144, 291, 558, 604
FauI CCCGC 1 cut(s) 485
Fnu4HI GCNGC 8 cut(s) 81, 107, 171, 189, 192, 222, 318, 524
FokI GGATG 3 cut(s) 11, 245, 410
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 8 cut(s) 81, 107, 171, 189, 192, 222, 318, 524
FspBI CTAG 3 cut(s) 176, 198, 672
GlaI GCGC 1 cut(s) 546
GluI GCNGC 8 cut(s) 81, 107, 171, 189, 192, 222, 318, 524
HaeIII GGCC 2 cut(s) 38, 776
HapII CCGG 2 cut(s) 119, 792
HhaI GCGC 1 cut(s) 547
Hin1II CATG 4 cut(s) 148, 295, 562, 608
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 1 cut(s) 764
HpaII CCGG 2 cut(s) 119, 792
HphI GGTGA 3 cut(s) 58, 737, 877
Hpy166II GTNNAC 4 cut(s) 307, 594, 745, 885
Hpy188I TCNGA 6 cut(s) 142, 266, 510, 661, 678, 763
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 4 cut(s) 307, 594, 745, 885
HpyAV CCTTC 2 cut(s) 593, 844
HpyCH4III ACNGT 8 cut(s) 211, 326, 404, 668, 725, 749, 876, 889
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 3 cut(s) 206, 253, 317
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 176, 185, 212, 221, 529, 627, 692
HpyF3I CTNAG 5 cut(s) 443, 611, 660, 728, 810
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 4 cut(s) 148, 295, 562, 608
HspAI GCGC 1 cut(s) 545
Kzo9I GATC 2 cut(s) 240, 269
LmnI GCTCC 4 cut(s) 103, 531, 542, 552
Lsp1109I GCAGC 7 cut(s) 67, 118, 200, 203, 208, 329, 535
MaeI CTAG 3 cut(s) 176, 198, 672
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 160, 713, 895
MalI GATC 2 cut(s) 242, 271
MboI GATC 2 cut(s) 240, 269
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 659
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 776
MlyI GAGTC 1 cut(s) 773
MmeI TCCRAC 2 cut(s) 445, 858
MnlI CCTC 6 cut(s) 59, 94, 260, 272, 550, 655
Mox20I TGGCCA 1 cut(s) 776
MscI TGGCCA 1 cut(s) 776
MseI TTAA 1 cut(s) 828
MslI CAYNNNNRTG 1 cut(s) 695
Msp20I TGGCCA 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 2 cut(s) 119, 792
MspR9I CCNGG 2 cut(s) 534, 839
MvaI CCWGG 2 cut(s) 534, 839
MvnI CGCG 2 cut(s) 545, 821
MwoI GCNNNNNNNGC 8 cut(s) 86, 176, 185, 212, 221, 529, 627, 692
NdeII GATC 2 cut(s) 240, 269
NlaIII CATG 4 cut(s) 148, 295, 562, 608
NlaIV GGNNCC 2 cut(s) 538, 790
NmuCI GTSAC 2 cut(s) 160, 895
NspI RCATGY 1 cut(s) 148
PaeR7I CTCGAG 1 cut(s) 98
PciI ACATGT 1 cut(s) 144
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 8 cut(s) 82, 108, 172, 190, 193, 223, 319, 525
PleI GAGTC 1 cut(s) 772
PpsI GAGTC 1 cut(s) 772
PscI ACATGT 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 659
Psp1406I AACGTT 1 cut(s) 462
Psp6I CCWGG 2 cut(s) 532, 837
PspGI CCWGG 2 cut(s) 532, 837
PspN4I GGNNCC 2 cut(s) 538, 790
PstI CTGCAG 1 cut(s) 319
PstNI CAGNNNCTG 1 cut(s) 529
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 4 cut(s) 202, 381, 670, 751
RsaNI GTAC 4 cut(s) 201, 380, 669, 750
RseI CAYNNNNRTG 1 cut(s) 695
SacI GAGCTC 1 cut(s) 659
SaqAI TTAA 1 cut(s) 828
SatI GCNGC 8 cut(s) 81, 107, 171, 189, 192, 222, 318, 524
Sau3AI GATC 2 cut(s) 240, 269
ScaI AGTACT 1 cut(s) 202
SchI GAGTC 1 cut(s) 773
ScrFI CCNGG 2 cut(s) 534, 839
SduI GDGCHC 1 cut(s) 659
SfcI CTRYAG 1 cut(s) 315
Sfr274I CTCGAG 1 cut(s) 98
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
SpeI ACTAGT 2 cut(s) 197, 671
Sse9I AATT 1 cut(s) 15
SsiI CCGC 4 cut(s) 171, 492, 543, 866
SspMI CTAG 3 cut(s) 176, 198, 672
SstI GAGCTC 1 cut(s) 659
StyD4I CCNGG 2 cut(s) 532, 837
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 8 cut(s) 211, 326, 404, 668, 725, 749, 876, 889
TaiI ACGT 1 cut(s) 465
TaqI TCGA 1 cut(s) 99
TasI AATT 1 cut(s) 15
TatI WGTACW 1 cut(s) 200
TauI GCSGC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TscAI CASTG 6 cut(s) 46, 138, 286, 601, 697, 879
TseFI GTSAC 2 cut(s) 160, 895
TseI GCWGC 7 cut(s) 80, 106, 188, 191, 221, 317, 523
Tsp45I GTSAC 2 cut(s) 160, 895
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 6 cut(s) 46, 138, 286, 601, 697, 879
Van91I CCANNNNNTGG 1 cut(s) 464
XapI RAATTY 1 cut(s) 15
XceI RCATGY 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 98
XspI CTAG 3 cut(s) 176, 198, 672
ZrmI AGTACT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.