MD01G1120000.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003115
Physical Location & Seq
Reverse (-)
23361200 .. 23361814
615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1120000.v1.1.491

Sequence Viewer

Length: 615 bp
ATGACGGGGATAGTCAACAACCCTGCAGTCAACGGCCAAGTACAGTTCGCCAAGCCCAATGGGGCAGTCCTACCATTTAACAATAGAGTACCCCAGTACAACAACAACAACGGTCTCATAAACAAAAATAACATCCCATTTCTCACAGGGCTTAGTGGAACAACACCAAACGTGGGACAAAATAACAATGGAAATGGTGGTGGGTTTGGAAACTTTCCGATAAATGGTGGGCAGCTCCTGCCAGGCTCTTCCACGCTCCAAAATCTCATGTTCGGAACAATGAGCGTATTTGATGATGAGTTGACTGAAAGGCATGAGCTTGGCTCTGGCTTGGTTGGCAAAGCACACGGTTTCTATAAAGTGAGCTCTGAGGACGGTACCAGTCAGACTATGGCTTTCACTGCTATGTTCCAAAGTGGGAGTTACACCGACAGTCTTGGCTTATTTGGGGTTCACCGGACATCTGTGTCAGAGTCTCACTTGGCTGTGATGGGAGGCACCGGAAAGTATGTCAATGCCAAAGGTTTCGCGTTGGTTAAGACCTTTCCGGCTTCCAACCAGCAAACTAATGGGGCGGAGACGCTGTTGCAGTTCACTGTTTATGTCACTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

205

Amino Acids

21.54

Weight (kDa)

6.9

Isoelectric Point (pI)

26.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 84 - 202 6.9e-25 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 466
Acc65I GGTACC 1 cut(s) 377
AccB1I GGYRCC 2 cut(s) 377, 497
AccII CGCG 1 cut(s) 530
AciI CCGC 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 34
AfaI GTAC 4 cut(s) 42, 90, 98, 379
AfiI CCNNNNNNNGG 2 cut(s) 173, 224
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 3 cut(s) 235, 319, 366
AluI AGCT 3 cut(s) 235, 319, 366
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 3 cut(s) 119, 480, 572
AlwNI CAGNNNCTG 1 cut(s) 238
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 1 cut(s) 232
Asp718I GGTACC 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 446
BanI GGYRCC 2 cut(s) 377, 497
BanII GRGCYC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 484
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 243
BcoDI GTCTC 3 cut(s) 119, 480, 572
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 1 cut(s) 233
BlsI GCNGC 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 379, 499
BmrFI CCNGG 1 cut(s) 243
BmrI ACTGGG 1 cut(s) 88
BmuI ACTGGG 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BsaI GGTCTC 1 cut(s) 119
BsaWI WCCGGW 2 cut(s) 456, 500
BsaXI ACNNNNNCTCC 2 cut(s) 569, 599
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 224
Bse1I ACTGG 2 cut(s) 94, 381
BseBI CCWGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 173, 224
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 2 cut(s) 94, 381
BseXI GCAGC 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 530
BshFI GGCC 1 cut(s) 36
BshNI GGYRCC 2 cut(s) 377, 497
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 3 cut(s) 457, 501, 548
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 2 cut(s) 173, 224
BsmAI GTCTC 3 cut(s) 119, 480, 572
BsmBI CGTCTC 1 cut(s) 572
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 1 cut(s) 36
Bso31I GGTCTC 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 368
BspACI CCGC 1 cut(s) 575
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 530
BspLI GGNNCC 2 cut(s) 379, 499
BspMAI CTGCAG 1 cut(s) 28
BspQI GCTCTTC 1 cut(s) 253
BspT107I GGYRCC 2 cut(s) 377, 497
BspTNI GGTCTC 1 cut(s) 119
BsrI ACTGG 2 cut(s) 94, 381
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 6 cut(s) 45, 113, 350, 377, 434, 598
Bst6I CTCTTC 1 cut(s) 253
BstAPI GCANNNNNTGC 1 cut(s) 238
BstDEI CTNAG 2 cut(s) 152, 369
BstF5I GGATG 1 cut(s) 132
BstFNI CGCG 1 cut(s) 530
BstMAI GTCTC 3 cut(s) 119, 480, 572
BstMWI GCNNNNNNNGC 3 cut(s) 238, 336, 401
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 1 cut(s) 244
BsuRI GGCC 1 cut(s) 36
BtsCI GGATG 1 cut(s) 132
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 399, 594
CaiI CAGNNNCTG 1 cut(s) 238
CseI GACGC 1 cut(s) 589
Csp6I GTAC 4 cut(s) 41, 89, 97, 378
CviAII CATG 2 cut(s) 268, 314
CviQI GTAC 4 cut(s) 41, 89, 97, 378
DdeI CTNAG 2 cut(s) 152, 369
DrdI GACNNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
EciI GGCGGA 1 cut(s) 590
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 368
Eco31I GGTCTC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 2 cut(s) 271, 317
FaiI YATR 8 cut(s) 119, 269, 315, 357, 392, 407, 510, 603
FaqI GGGAC 1 cut(s) 189
FatI CATG 2 cut(s) 267, 313
Fnu4HI GCNGC 1 cut(s) 233
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 1 cut(s) 233
GluI GCNGC 1 cut(s) 233
HaeIII GGCC 1 cut(s) 36
HapII CCGG 3 cut(s) 457, 501, 548
HgaI GACGC 1 cut(s) 589
Hin1II CATG 2 cut(s) 271, 317
HincII GTYRAC 3 cut(s) 16, 31, 303
HindII GTYRAC 3 cut(s) 16, 31, 303
HinfI GANTC 1 cut(s) 473
HpaII CCGG 3 cut(s) 457, 501, 548
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 5 cut(s) 16, 31, 303, 454, 594
Hpy188I TCNGA 5 cut(s) 219, 275, 370, 387, 472
Hpy8I GTNNAC 5 cut(s) 16, 31, 303, 454, 594
HpyCH4III ACNGT 6 cut(s) 45, 113, 350, 377, 434, 598
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 26, 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 336, 401
HpyF3I CTNAG 2 cut(s) 152, 369
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 271, 317
KpnI GGTACC 1 cut(s) 381
LguI GCTCTTC 1 cut(s) 253
LmnI GCTCC 2 cut(s) 240, 261
Lsp1109I GCAGC 1 cut(s) 244
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 422, 604
MboII GAAGA 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 368
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 579
MnlI CCTC 2 cut(s) 364, 488
MseI TTAA 2 cut(s) 78, 537
MslI CAYNNNNRTG 1 cut(s) 404
MspI CCGG 3 cut(s) 457, 501, 548
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 3 cut(s) 238, 336, 401
NlaIII CATG 2 cut(s) 271, 317
NlaIV GGNNCC 2 cut(s) 379, 499
NmuCI GTSAC 1 cut(s) 604
PciSI GCTCTTC 1 cut(s) 253
PkrI GCNGC 1 cut(s) 234
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 379, 499
PstI CTGCAG 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 238
RsaI GTAC 4 cut(s) 42, 90, 98, 379
RsaNI GTAC 4 cut(s) 41, 89, 97, 378
RseI CAYNNNNRTG 1 cut(s) 404
SacI GAGCTC 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 253
SaqAI TTAA 2 cut(s) 78, 537
SatI GCNGC 1 cut(s) 233
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 1 cut(s) 368
SetI ASST 6 cut(s) 174, 237, 321, 368, 526, 545
SfcI CTRYAG 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 404
SsiI CCGC 1 cut(s) 575
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 241
TaaI ACNGT 6 cut(s) 45, 113, 350, 377, 434, 598
TaiI ACGT 1 cut(s) 174
TatI WGTACW 2 cut(s) 40, 96
Tru1I TTAA 2 cut(s) 78, 537
Tru9I TTAA 2 cut(s) 78, 537
TscAI CASTG 2 cut(s) 406, 601
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 1 cut(s) 604
TspRI CASTG 2 cut(s) 406, 601
XcmI CCANNNNNNNNNTGG 2 cut(s) 388, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.