Rw1G029310

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003115
Physical Location & Seq
Reverse (-)
56991064 .. 56991939
876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029310.1

Sequence Viewer

Length: 876 bp
ATGGAAGCTCACAAAATGTTCTCAGTCACCCACCTACTATTCCTCACCCTCACCATTTTATCTGCCACCTCAGCGCGAACTCTCGAGGAGCAGCCACTGGTTCCTGTTGTGCCTCTGGTGGTGTCGAATACAATTGGCACACCTTCCATCAATGCTGCACCAGTGGTCACACCTTCAGCTTCACAGCCTGCCACTGTGGCTGATGCTGATGACCACCATTCCTTAGTCTTCTTCCTGCACGACATTCTAGGTGGCACGAACCCCTCAGCTAGAGCCATGACAGGGATAGTCAACAGCCCTGCAGTCAATGGACAAGTACCATTTGCCAAACCAAATGGAGCAGTCCTGCCATTTAACAATGGAATACCCCAGAACAACAACAACAACGGTATTCTAAGCAACAACAACATCCCGTTTCTCACTGGACTTAGTGGAGCAACGCAAAATGTGGTACAAAACAATAACAACAACAATTTCATCAATGGTGGGCAGCTCTTTCCAGGAGGCTCCACGCTCCAAAACCTCATGTTCGGCACAATGAGTGTGTTCGATGATGAGCTAACTGAAGGGCATGAGCTCGGGTCTGGTTTGGTTGGCAAGGCACAAGGGTTCTATGTGGTGAGTTCTGAAGATGGTACGAGTCAGACTATGGCTTTCACTGCTATGTTTCAAAATGGGGGTTATGCTGATAGCCTTAGCTTCTTTGGGGTCCATCGTGTGGCTGCGTCAGAGTCTCACCTGGCTATCATGGGTGGCACCGGAAAGTATGTTGATGCGAAAGGGTTTGCCATTGTCAAGACATTCCCGGCTACCAATGAGCATCAAACTGATGGAGCAGAGACCTTGTTGCAGTTCACTGTTTATATTACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

291

Amino Acids

30.61

Weight (kDa)

5.05

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 154 - 289 1.9e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 755
AccB7I CCANNNNNTGG 1 cut(s) 718
AccII CGCG 1 cut(s) 76
AcuI CTGAAG 3 cut(s) 159, 585, 648
AfaI GTAC 3 cut(s) 318, 453, 637
AfiI CCNNNNNNNGG 2 cut(s) 282, 718
AgsI TTSAA 1 cut(s) 671
AjnI CCWGG 2 cut(s) 499, 738
AluBI AGCT 7 cut(s) 8, 179, 269, 493, 559, 577, 699
AluI AGCT 7 cut(s) 8, 179, 269, 493, 559, 577, 699
Alw21I GWGCWC 1 cut(s) 579
Alw26I GTCTC 2 cut(s) 738, 833
AlwNI CAGNNNCTG 1 cut(s) 97
Ama87I CYCGRG 2 cut(s) 83, 578
ApeKI GCWGC 4 cut(s) 91, 155, 490, 722
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 709
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 5 cut(s) 19, 37, 43, 631, 728
AvaI CYCGRG 2 cut(s) 83, 578
AvaII GGWCC 1 cut(s) 709
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 579
BbvCI CCTCAGC 2 cut(s) 70, 265
BbvI GCAGC 4 cut(s) 103, 142, 502, 709
BccI CCATC 4 cut(s) 155, 626, 720, 824
BciT130I CCWGG 2 cut(s) 501, 740
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 2 cut(s) 738, 833
BfaI CTAG 3 cut(s) 248, 270, 874
BfmI CTRYAG 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 4 cut(s) 92, 156, 491, 723
BlsI GCNGC 4 cut(s) 93, 157, 492, 724
Bme1390I CCNGG 3 cut(s) 501, 740, 806
Bme18I GGWCC 1 cut(s) 709
BmeT110I CYCGRG 2 cut(s) 83, 578
BmgT120I GGNCC 1 cut(s) 709
BmiI GGNNCC 4 cut(s) 102, 508, 710, 757
BmrFI CCNGG 3 cut(s) 501, 740, 806
BmsI GCATC 3 cut(s) 193, 763, 829
BpiI GAAGAC 1 cut(s) 220
Bpu10I CCTNAGC 3 cut(s) 70, 265, 695
BpuMI CCSGG 1 cut(s) 806
BsaI GGTCTC 1 cut(s) 833
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 718
Bse1I ACTGG 3 cut(s) 102, 161, 427
BseBI CCWGG 2 cut(s) 501, 740
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 282, 718
BseMII CTCAG 3 cut(s) 36, 84, 279
BseNI ACTGG 3 cut(s) 102, 161, 427
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 4 cut(s) 103, 142, 502, 709
BsgI GTGCAG 2 cut(s) 141, 221
Bsh1236I CGCG 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 755
BsiHKAI GWGCWC 1 cut(s) 579
BsiHKCI CYCGRG 2 cut(s) 83, 578
BsiSI CCGG 2 cut(s) 759, 806
BslI CCNNNNNNNGG 2 cut(s) 282, 718
BsmAI GTCTC 2 cut(s) 738, 833
Bso31I GGTCTC 1 cut(s) 833
BsoBI CYCGRG 2 cut(s) 83, 578
Bsp1286I GDGCHC 1 cut(s) 579
BspCNI CTCAG 3 cut(s) 35, 83, 278
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 102, 508, 710, 757
BspMAI CTGCAG 1 cut(s) 304
BspT107I GGYRCC 1 cut(s) 755
BspTNI GGTCTC 1 cut(s) 833
BsrI ACTGG 3 cut(s) 102, 161, 427
Bst2UI CCWGG 2 cut(s) 501, 740
Bst4CI ACNGT 3 cut(s) 196, 389, 859
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 7 cut(s) 22, 70, 223, 265, 395, 428, 695
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 76
BstMAI GTCTC 2 cut(s) 738, 833
BstMWI GCNNNNNNNGC 3 cut(s) 71, 197, 659
BstNI CCWGG 2 cut(s) 501, 740
BstSCI CCNGG 3 cut(s) 499, 738, 804
BstSFI CTRYAG 1 cut(s) 300
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 4 cut(s) 103, 142, 502, 709
BstV2I GAAGAC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 6 cut(s) 95, 168, 192, 420, 657, 855
Cac8I GCNNGC 1 cut(s) 189
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 709
CseI GACGC 1 cut(s) 714
Csp6I GTAC 3 cut(s) 317, 452, 636
CviAII CATG 4 cut(s) 277, 526, 572, 748
CviQI GTAC 3 cut(s) 317, 452, 636
DdeI CTNAG 7 cut(s) 22, 70, 223, 265, 395, 428, 695
Ecl136II GAGCTC 1 cut(s) 577
Eco24I GRGCYC 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 1 cut(s) 709
Eco53kI GAGCTC 1 cut(s) 577
Eco57I CTGAAG 3 cut(s) 159, 585, 648
Eco88I CYCGRG 2 cut(s) 83, 578
EcoICRI GAGCTC 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 499, 738
EcoT38I GRGCYC 1 cut(s) 579
FaeI CATG 4 cut(s) 280, 529, 575, 751
FatI CATG 4 cut(s) 276, 525, 571, 747
Fnu4HI GCNGC 4 cut(s) 92, 156, 491, 723
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 1 cut(s) 579
Fsp4HI GCNGC 4 cut(s) 92, 156, 491, 723
FspBI CTAG 3 cut(s) 248, 270, 874
GlaI GCGC 1 cut(s) 75
GluI GCNGC 4 cut(s) 92, 156, 491, 723
HapII CCGG 2 cut(s) 759, 806
HgaI GACGC 1 cut(s) 714
HhaI GCGC 1 cut(s) 76
Hin1II CATG 4 cut(s) 280, 529, 575, 751
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 640, 731
HpaII CCGG 2 cut(s) 759, 806
HphI GGTGA 5 cut(s) 19, 37, 43, 631, 728
Hpy166II GTNNAC 2 cut(s) 292, 855
Hpy188I TCNGA 3 cut(s) 628, 645, 730
Hpy188III TCNNGA 2 cut(s) 83, 796
Hpy8I GTNNAC 2 cut(s) 292, 855
HpyAV CCTTC 3 cut(s) 153, 183, 560
HpyCH4III ACNGT 3 cut(s) 196, 389, 859
HpyCH4V TGCA 4 cut(s) 158, 238, 302, 850
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 197, 659
HpyF3I CTNAG 7 cut(s) 22, 70, 223, 265, 395, 428, 695
Hsp92II CATG 4 cut(s) 280, 529, 575, 751
HspAI GCGC 1 cut(s) 74
LmnI GCTCC 6 cut(s) 88, 338, 434, 512, 519, 833
Lsp1109I GCAGC 4 cut(s) 103, 142, 502, 709
LweI GCATC 3 cut(s) 193, 763, 829
MaeI CTAG 3 cut(s) 248, 270, 874
MaeIII GTNAC 2 cut(s) 25, 166
MboII GAAGA 3 cut(s) 220, 223, 641
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 579
MluCI AATT 2 cut(s) 132, 472
MlyI GAGTC 2 cut(s) 649, 740
MnlI CCTC 8 cut(s) 53, 59, 79, 79, 123, 274, 497, 533
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 662
MspI CCGG 2 cut(s) 759, 806
MspR9I CCNGG 3 cut(s) 501, 740, 806
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 2 cut(s) 501, 740
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 3 cut(s) 71, 197, 659
NciI CCSGG 1 cut(s) 806
NlaIII CATG 4 cut(s) 280, 529, 575, 751
NlaIV GGNNCC 4 cut(s) 102, 508, 710, 757
NmuCI GTSAC 2 cut(s) 25, 166
PaeR7I CTCGAG 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 718
PfoI TCCNGGA 1 cut(s) 499
PkrI GCNGC 4 cut(s) 93, 157, 492, 724
PleI GAGTC 2 cut(s) 648, 739
PpsI GAGTC 2 cut(s) 648, 739
Psp124BI GAGCTC 1 cut(s) 579
Psp6I CCWGG 2 cut(s) 499, 738
PspGI CCWGG 2 cut(s) 499, 738
PspN4I GGNNCC 4 cut(s) 102, 508, 710, 757
PspPI GGNCC 1 cut(s) 709
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 97
RsaI GTAC 3 cut(s) 318, 453, 637
RsaNI GTAC 3 cut(s) 317, 452, 636
RseI CAYNNNNRTG 1 cut(s) 662
SacI GAGCTC 1 cut(s) 579
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 4 cut(s) 92, 156, 491, 723
Sau96I GGNCC 1 cut(s) 709
SchI GAGTC 2 cut(s) 649, 740
ScrFI CCNGG 3 cut(s) 501, 740, 806
SduI GDGCHC 1 cut(s) 579
SfaNI GCATC 3 cut(s) 193, 763, 829
SfcI CTRYAG 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 709
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 2 cut(s) 132, 472
SspMI CTAG 3 cut(s) 248, 270, 874
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 3 cut(s) 499, 738, 804
TaaI ACNGT 3 cut(s) 196, 389, 859
TaqI TCGA 3 cut(s) 84, 125, 549
TasI AATT 2 cut(s) 132, 472
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 6 cut(s) 102, 168, 199, 427, 664, 862
TseFI GTSAC 2 cut(s) 25, 166
TseI GCWGC 4 cut(s) 91, 155, 490, 722
Tsp45I GTSAC 2 cut(s) 25, 166
TspDTI ATGAA 1 cut(s) 466
TspRI CASTG 6 cut(s) 102, 168, 199, 427, 664, 862
Van91I CCANNNNNTGG 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 709
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 3 cut(s) 248, 270, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.