MD03G1102000.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003115
Physical Location & Seq
Forward (+)
8739165 .. 8740111
947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1102000.v1.1.491

Sequence Viewer

Length: 885 bp
ATGGCAACCCGGAAATCGACTCCCTTGGCCCTCAAGCTCACAATCTGGCTGCTGCTTCTAGCTCTTGCCATGCCATGCGCCACCTCTGCCCGAATCCTCGATGAGGAAAACCCTGCGGCCCCCGAGGAGACTGATACAACTCAAACACAAACACCTGTTTCCAATGTGCCACCTCCTCAGGTAGCTACAAATCCAGCAGGCCCTGCTGCAGCTACAAGTGCCACAGTTGGCAATGCAGCATCCTATCATCCACTAACCTTCTTTTTGCATGACATTATCGGTGGATCAAACCCCTCGGCTAGAGCTGTCACTGGAATTGTGGCCAATCCGGCAGTCAGCGGTCAAGTCCCTTCTGCGAACAACAACAACAATGGAATCATCAACAACAACAACGTCCCCTTCTTCACCGGCCTTGGTGGAAACGCCGGCAACAACTTGATCCAAAACAATGGCAACAACAACATCATTGGTGGGAATGGGCTTCCGTACCTGAACGGGGCTCAGCTTCCCCCTGGAATCACCCTTCAGAAGCTTATGTTTGGGACCCTGACAGTTTTCGACGATGAGCTGACTGAAGGGCATGAGCTTGGATCTGGCTTGCTCGGCAGGGCACAAGGGTTTTACGTGGCGAGTTCTGAAGATGGGAGGAGCCAGACCATTGCCTTCACAGCCATGTTTCAGAGTGGAGGTTATGCTGATAGCCTCACCTTCTTCGGTGTGCATCGGGTGGCAGCGTCGGAGTCTCATCTTGCAGTCATGGGAGGAACTGGCAAGTATCTCAATGCAAAGGGTTATGCTCTTGTTAAGACTATTCCTGCAACAAATCAGCAAAACACTGATGGAGTTGACGCTGTGCTGCAGTTTACCACGTATCTTACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

295

Amino Acids

30.49

Weight (kDa)

5.25

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 172 - 291 1.3e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 116, 339
AclWI GGATC 3 cut(s) 292, 433, 598
AcoI YGGCCR 1 cut(s) 321
AcuI CTGAAG 3 cut(s) 509, 594, 657
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 2 cut(s) 103, 496
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 9 cut(s) 37, 62, 185, 212, 305, 505, 532, 568, 586
AluI AGCT 9 cut(s) 37, 62, 185, 212, 305, 505, 532, 568, 586
Alw26I GTCTC 2 cut(s) 122, 747
AlwI GGATC 3 cut(s) 292, 433, 598
AlwNI CAGNNNCTG 1 cut(s) 203
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 5 cut(s) 27, 117, 199, 321, 409
ApeKI GCWGC 7 cut(s) 49, 52, 206, 209, 236, 731, 856
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 4 cut(s) 28, 118, 200, 543
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 397, 511, 697
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 543
AxyI CCTNAGG 1 cut(s) 177
BaeGI GKGCMC 1 cut(s) 613
BalI TGGCCA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 502
BbvI GCAGC 7 cut(s) 36, 39, 193, 221, 248, 743, 843
BccI CCATC 2 cut(s) 635, 833
BciT130I CCWGG 1 cut(s) 513
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 2 cut(s) 122, 747
BfaI CTAG 3 cut(s) 59, 300, 883
BfmI CTRYAG 2 cut(s) 207, 857
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 8 cut(s) 50, 53, 117, 207, 210, 237, 732, 857
BlpI GCTNAGC 1 cut(s) 501
BlsI GCNGC 8 cut(s) 51, 54, 118, 208, 211, 238, 733, 858
Bme1390I CCNGG 2 cut(s) 10, 513
Bme18I GGWCC 1 cut(s) 543
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 4 cut(s) 28, 118, 200, 543
BmiI GGNNCC 4 cut(s) 120, 544, 545, 650
BmrFI CCNGG 2 cut(s) 10, 513
BmsI GCATC 2 cut(s) 248, 730
Bpu1102I GCTNAGC 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 10
BsaAI YACGTR 2 cut(s) 625, 870
BsaJI CCNNGG 5 cut(s) 24, 123, 294, 412, 511
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 496
Bse118I RCCGGY 2 cut(s) 407, 425
Bse1I ACTGG 2 cut(s) 316, 772
Bse21I CCTNAGG 1 cut(s) 177
Bse3DI GCAATG 2 cut(s) 238, 657
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 5 cut(s) 24, 123, 294, 412, 511
BseGI GGATG 2 cut(s) 239, 247
BseLI CCNNNNNNNGG 2 cut(s) 103, 496
BseMI GCAATG 2 cut(s) 238, 657
BseMII CTCAG 2 cut(s) 191, 515
BseNI ACTGG 2 cut(s) 316, 772
BseRI GAGGAG 3 cut(s) 140, 165, 661
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 7 cut(s) 36, 39, 193, 221, 248, 743, 843
BshFI GGCC 5 cut(s) 29, 119, 201, 323, 411
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 4 cut(s) 10, 329, 408, 426
BslFI GGGAC 3 cut(s) 332, 380, 556
BslI CCNNNNNNNGG 2 cut(s) 103, 496
BsmAI GTCTC 2 cut(s) 122, 747
BsmFI GGGAC 3 cut(s) 332, 380, 556
BsnI GGCC 5 cut(s) 29, 119, 201, 323, 411
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 502, 613
Bsp143I GATC 3 cut(s) 284, 438, 590
Bsp1720I GCTNAGC 1 cut(s) 501
BspACI CCGC 2 cut(s) 116, 339
BspANI GGCC 5 cut(s) 29, 119, 201, 323, 411
BspCNI CTCAG 2 cut(s) 190, 514
BspLI GGNNCC 4 cut(s) 120, 544, 545, 650
BspMAI CTGCAG 2 cut(s) 211, 861
BspPI GGATC 3 cut(s) 292, 433, 598
BsrDI GCAATG 2 cut(s) 238, 657
BsrFI RCCGGY 2 cut(s) 407, 425
BsrI ACTGG 2 cut(s) 316, 772
BssAI RCCGGY 2 cut(s) 407, 425
BssECI CCNNGG 5 cut(s) 24, 123, 294, 412, 511
BssMI GATC 3 cut(s) 284, 438, 590
BssT1I CCWWGG 2 cut(s) 24, 412
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 226, 553
BstAPI GCANNNNNTGC 1 cut(s) 203
BstBAI YACGTR 2 cut(s) 625, 870
BstC8I GCNNGC 3 cut(s) 199, 427, 599
BstDEI CTNAG 2 cut(s) 177, 501
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 2 cut(s) 239, 247
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 3 cut(s) 287, 441, 593
BstMAI GTCTC 2 cut(s) 122, 747
BstMBI GATC 3 cut(s) 284, 438, 590
BstMWI GCNNNNNNNGC 6 cut(s) 86, 203, 218, 329, 603, 668
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 2 cut(s) 8, 511
BstSFI CTRYAG 2 cut(s) 207, 857
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 7 cut(s) 36, 39, 193, 221, 248, 743, 843
BstX2I RGATCY 1 cut(s) 590
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 590
Bsu36I CCTNAGG 1 cut(s) 177
BsuRI GGCC 5 cut(s) 29, 119, 201, 323, 411
BtsCI GGATG 2 cut(s) 239, 247
BtsIMutI CAGTG 2 cut(s) 309, 834
Cac8I GCNNGC 3 cut(s) 199, 427, 599
CaiI CAGNNNCTG 1 cut(s) 203
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 2 cut(s) 407, 425
Cfr13I GGNCC 4 cut(s) 28, 118, 200, 543
CseI GACGC 2 cut(s) 723, 857
Csp6I GTAC 1 cut(s) 487
CviAII CATG 6 cut(s) 70, 75, 269, 581, 673, 757
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 2 cut(s) 177, 501
DpnI GATC 3 cut(s) 286, 440, 592
DpnII GATC 3 cut(s) 284, 438, 590
EaeI YGGCCR 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 24, 412
Eco24I GRGCYC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 543
Eco57I CTGAAG 3 cut(s) 509, 594, 657
Eco81I CCTNAGG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 122
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 2 cut(s) 200, 543
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 24, 412
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 2 cut(s) 24, 412
FaeI CATG 6 cut(s) 73, 78, 272, 584, 676, 760
FaiI YATR 9 cut(s) 71, 76, 270, 536, 582, 674, 693, 758, 795
FaqI GGGAC 3 cut(s) 332, 380, 556
FatI CATG 6 cut(s) 69, 74, 268, 580, 672, 756
Fnu4HI GCNGC 8 cut(s) 50, 53, 117, 207, 210, 237, 732, 857
FokI GGATG 2 cut(s) 226, 234
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 8 cut(s) 50, 53, 117, 207, 210, 237, 732, 857
FspBI CTAG 3 cut(s) 59, 300, 883
GlaI GCGC 1 cut(s) 79
GluI GCNGC 8 cut(s) 50, 53, 117, 207, 210, 237, 732, 857
HaeIII GGCC 5 cut(s) 29, 119, 201, 323, 411
HapII CCGG 4 cut(s) 10, 329, 408, 426
HgaI GACGC 2 cut(s) 723, 857
HhaI GCGC 1 cut(s) 80
Hin1II CATG 6 cut(s) 73, 78, 272, 584, 676, 760
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 5 cut(s) 19, 93, 375, 516, 740
HpaII CCGG 4 cut(s) 10, 329, 408, 426
HphI GGTGA 3 cut(s) 397, 511, 697
Hpy166II GTNNAC 2 cut(s) 847, 864
Hpy188I TCNGA 4 cut(s) 528, 637, 681, 739
Hpy8I GTNNAC 2 cut(s) 847, 864
Hpy99I CGWCG 2 cut(s) 563, 739
HpyAV CCTTC 7 cut(s) 268, 360, 409, 533, 569, 673, 718
HpyCH4III ACNGT 2 cut(s) 226, 553
HpyCH4IV ACGT 3 cut(s) 393, 624, 869
HpyCH4V TGCA 8 cut(s) 209, 236, 268, 721, 752, 785, 818, 859
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 203, 218, 329, 603, 668
HpyF3I CTNAG 2 cut(s) 177, 501
HpySE526I ACGT 3 cut(s) 393, 624, 869
Hsp92II CATG 6 cut(s) 73, 78, 272, 584, 676, 760
HspAI GCGC 1 cut(s) 78
KflI GGGWCCC 1 cut(s) 543
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Kzo9I GATC 3 cut(s) 284, 438, 590
LmnI GCTCC 1 cut(s) 648
Lsp1109I GCAGC 7 cut(s) 36, 39, 193, 221, 248, 743, 843
LweI GCATC 2 cut(s) 248, 730
MaeI CTAG 3 cut(s) 59, 300, 883
MaeII ACGT 3 cut(s) 393, 624, 869
MaeIII GTNAC 1 cut(s) 307
MalI GATC 3 cut(s) 286, 440, 592
MboI GATC 3 cut(s) 284, 438, 590
MboII GAAGA 3 cut(s) 394, 650, 703
MflI RGATCY 1 cut(s) 590
MhlI GDGCHC 2 cut(s) 502, 613
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 1 cut(s) 315
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 2 cut(s) 13, 749
MmeI TCCRAC 1 cut(s) 717
Mox20I TGGCCA 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 425
MscI TGGCCA 1 cut(s) 323
MseI TTAA 1 cut(s) 804
MslI CAYNNNNRTG 1 cut(s) 671
Msp20I TGGCCA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 4 cut(s) 10, 329, 408, 426
MspR9I CCNGG 2 cut(s) 10, 513
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 6 cut(s) 86, 203, 218, 329, 603, 668
NaeI GCCGGC 1 cut(s) 427
NciI CCSGG 1 cut(s) 10
NdeII GATC 3 cut(s) 284, 438, 590
NgoMIV GCCGGC 1 cut(s) 425
NlaIII CATG 6 cut(s) 73, 78, 272, 584, 676, 760
NlaIV GGNNCC 4 cut(s) 120, 544, 545, 650
NmeAIII GCCGAG 2 cut(s) 275, 582
NmuCI GTSAC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 427
PfeI GAWTC 3 cut(s) 93, 375, 516
PkrI GCNGC 8 cut(s) 51, 54, 118, 208, 211, 238, 733, 858
PleI GAGTC 2 cut(s) 13, 748
PpsI GAGTC 2 cut(s) 13, 748
Ppu21I YACGTR 2 cut(s) 625, 870
PpuMI RGGWCCY 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 4 cut(s) 120, 544, 545, 650
PspPI GGNCC 4 cut(s) 28, 118, 200, 543
PspPPI RGGWCCY 1 cut(s) 543
PstI CTGCAG 2 cut(s) 211, 861
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 590
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 1 cut(s) 804
SatI GCNGC 8 cut(s) 50, 53, 117, 207, 210, 237, 732, 857
Sau3AI GATC 3 cut(s) 284, 438, 590
Sau96I GGNCC 4 cut(s) 28, 118, 200, 543
SchI GAGTC 2 cut(s) 13, 749
ScrFI CCNGG 2 cut(s) 10, 513
SduI GDGCHC 2 cut(s) 502, 613
SfaNI GCATC 2 cut(s) 248, 730
SfcI CTRYAG 2 cut(s) 207, 857
SinI GGWCC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 1 cut(s) 315
SsiI CCGC 2 cut(s) 116, 339
SspMI CTAG 3 cut(s) 59, 300, 883
StyD4I CCNGG 2 cut(s) 8, 511
StyI CCWWGG 2 cut(s) 24, 412
TaaI ACNGT 2 cut(s) 226, 553
TaiI ACGT 3 cut(s) 396, 627, 872
TaqI TCGA 3 cut(s) 17, 99, 558
TasI AATT 1 cut(s) 315
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 3 cut(s) 93, 375, 516
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TscAI CASTG 2 cut(s) 316, 841
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 7 cut(s) 49, 52, 206, 209, 236, 731, 856
Tsp45I GTSAC 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 2 cut(s) 316, 841
VpaK11BI GGWCC 1 cut(s) 543
XagI CCTNNNNNAGG 1 cut(s) 101
XspI CTAG 3 cut(s) 59, 300, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.