Rw5G020350

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003115
Physical Location & Seq
Reverse (-)
26814440 .. 26822957
8518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020350.1

Sequence Viewer

Length: 726 bp
ATGGCAACCCAAAACTCAACTTCGCTGCCCCTCAAGGCCATAACATATATAAAAATTATCAAATTGGTTGCTGTCACCATTAACCAAAACGTGGGGGTAAAATGGTATCCAAAGCCCACTTTTGTGGTGTGCACAGTGGCCAATGGAGAGGCCTACTATCTGCTTTCAATCTTTCTGCATGATATATATATAATTGCTGTGACTGGGATTGAGACCGACCCAGCTGCCAATGGCCAAGTGCCCTTTGCCAAACCCAATGAGGCAGTTGTCCAAGGCTGGAATAATGGCATTCCCCAAAACAACAACAACAATGGTATCATCAACAATAATAATGTCCCATTCTTCACTGGATTGTTCCCCGTTGTGAACGGGGCCCAGCTCTTGCCAGGAGAGTCCTTTCAGAAGCTCATTTCACTGATCGAAGGTCATCAACTTGGCTCTGGCTTGTTGGGCAAGGCACAAGGATTTTACATCTCTAGTTCCGAAGATGGGAGTAGCCAGACCATTTCTTTCACTGCAATGTTTGAGAGTGGTGGGTATGCTGATAGCCTCACATTCTTTGGGGTTCACAAATCGAGTAAGTCTCACCTTGCAGTTATGGGAGGGACCAGGAAGTACTTGAATGCAAAAGGGTATGCTGTGGTTAAGACCATTAATGGAGTGAATGGGCAAAATACTGATGGAATTGACACTGTTGTTCAATTTACTGTGTATCTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

241

Amino Acids

25.87

Weight (kDa)

7.79

Isoelectric Point (pI)

23.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 139 - 239 4.2e-16 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 91
AcoI YGGCCR 2 cut(s) 138, 232
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 2 cut(s) 91, 489
AgsI TTSAA 3 cut(s) 168, 622, 701
AjnI CCWGG 2 cut(s) 385, 608
AleI CACNNNNGTG 1 cut(s) 122
AluBI AGCT 3 cut(s) 224, 379, 406
AluI AGCT 3 cut(s) 224, 379, 406
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 2 cut(s) 206, 588
Alw44I GTGCAC 1 cut(s) 130
AoxI GGCC 5 cut(s) 36, 138, 150, 232, 372
ApaI GGGCCC 1 cut(s) 376
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 25, 224
AseI ATTAAT 1 cut(s) 654
AspS9I GGNCC 3 cut(s) 372, 373, 606
AsuHPI GGTGA 2 cut(s) 67, 578
AvaII GGWCC 1 cut(s) 606
BaeGI GKGCMC 3 cut(s) 134, 243, 376
BaeI ACNNNNGTAYC 2 cut(s) 298, 331
BalI TGGCCA 2 cut(s) 140, 234
BanII GRGCYC 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 12, 211
BccI CCATC 2 cut(s) 482, 674
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BciT130I CCWGG 2 cut(s) 387, 610
BciVI GTATCC 1 cut(s) 117
BcoDI GTCTC 2 cut(s) 206, 588
BfaI CTAG 2 cut(s) 477, 724
BfuI GTATCC 1 cut(s) 117
BisI GCNGC 2 cut(s) 26, 225
BlsI GCNGC 2 cut(s) 27, 226
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 2 cut(s) 387, 610
Bme18I GGWCC 1 cut(s) 606
BmgT120I GGNCC 3 cut(s) 372, 373, 606
BmiI GGNNCC 3 cut(s) 373, 374, 607
BmrFI CCNGG 2 cut(s) 387, 610
BmrI ACTGGG 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 213
BplI GAGNNNNNCTC 2 cut(s) 568, 600
BpuEI CTTGAG 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 271
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 489
Bse1I ACTGG 2 cut(s) 208, 352
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 2 cut(s) 387, 610
BseDI CCNNGG 1 cut(s) 271
BseLI CCNNNNNNNGG 2 cut(s) 91, 489
BseMI GCAATG 1 cut(s) 525
BseNI ACTGG 2 cut(s) 208, 352
BseSI GKGCMC 3 cut(s) 134, 243, 376
BseXI GCAGC 2 cut(s) 12, 211
BseYI CCCAGC 2 cut(s) 220, 375
BshFI GGCC 5 cut(s) 38, 140, 152, 234, 374
BsiHKAI GWGCWC 1 cut(s) 134
BslFI GGGAC 2 cut(s) 320, 619
BslI CCNNNNNNNGG 2 cut(s) 91, 489
BsmAI GTCTC 2 cut(s) 206, 588
BsmFI GGGAC 2 cut(s) 320, 619
BsmI GAATGC 2 cut(s) 288, 628
BsnI GGCC 5 cut(s) 38, 140, 152, 234, 374
Bso31I GGTCTC 1 cut(s) 206
Bsp120I GGGCCC 1 cut(s) 372
Bsp1286I GDGCHC 3 cut(s) 134, 243, 376
Bsp143I GATC 1 cut(s) 417
BspANI GGCC 5 cut(s) 38, 140, 152, 234, 374
BspLI GGNNCC 3 cut(s) 373, 374, 607
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 525
BsrI ACTGG 2 cut(s) 208, 352
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 2 cut(s) 387, 610
Bst4CI ACNGT 3 cut(s) 136, 694, 709
BstDEI CTNAG 1 cut(s) 716
BstKTI GATC 1 cut(s) 420
BstMAI GTCTC 2 cut(s) 206, 588
BstMBI GATC 1 cut(s) 417
BstMWI GCNNNNNNNGC 1 cut(s) 450
BstNI CCWGG 2 cut(s) 387, 610
BstSCI CCNGG 2 cut(s) 385, 608
BstSLI GKGCMC 3 cut(s) 134, 243, 376
BstV1I GCAGC 2 cut(s) 12, 211
BstXI CCANNNNNNTGG 1 cut(s) 124
BsuI GTATCC 1 cut(s) 117
BsuRI GGCC 5 cut(s) 38, 140, 152, 234, 374
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 5 cut(s) 141, 345, 413, 513, 690
Cfr13I GGNCC 3 cut(s) 372, 373, 606
Csp6I GTAC 1 cut(s) 616
CviAII CATG 1 cut(s) 179
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 1 cut(s) 716
DpnI GATC 1 cut(s) 419
DpnII GATC 1 cut(s) 417
EaeI YGGCCR 2 cut(s) 138, 232
Eco130I CCWWGG 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 385, 608
EcoT14I CCWWGG 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 1 cut(s) 182
FaqI GGGAC 2 cut(s) 320, 619
FatI CATG 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 26, 225
FriOI GRGCYC 1 cut(s) 376
Fsp4HI GCNGC 2 cut(s) 26, 225
FspBI CTAG 2 cut(s) 477, 724
GluI GCNGC 2 cut(s) 26, 225
GsaI CCCAGC 2 cut(s) 224, 379
HaeIII GGCC 5 cut(s) 38, 140, 152, 234, 374
Hin1II CATG 1 cut(s) 182
HinfI GANTC 1 cut(s) 392
HphI GGTGA 2 cut(s) 67, 578
Hpy166II GTNNAC 3 cut(s) 132, 367, 568
Hpy188I TCNGA 2 cut(s) 402, 484
Hpy8I GTNNAC 3 cut(s) 132, 367, 568
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 3 cut(s) 136, 694, 709
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 5 cut(s) 132, 178, 518, 593, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 450
HpyF3I CTNAG 1 cut(s) 716
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 1 cut(s) 182
Kzo9I GATC 1 cut(s) 417
Lsp1109I GCAGC 2 cut(s) 12, 211
MaeI CTAG 2 cut(s) 477, 724
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 73, 199, 719
MalI GATC 1 cut(s) 419
MboI GATC 1 cut(s) 417
MboII GAAGA 2 cut(s) 334, 497
MhlI GDGCHC 3 cut(s) 134, 243, 376
MlsI TGGCCA 2 cut(s) 140, 234
MluCI AATT 5 cut(s) 54, 62, 192, 684, 701
MluNI TGGCCA 2 cut(s) 140, 234
MlyI GAGTC 1 cut(s) 401
MnlI CCTC 5 cut(s) 41, 142, 253, 560, 596
Mox20I TGGCCA 2 cut(s) 140, 234
MscI TGGCCA 2 cut(s) 140, 234
MseI TTAA 3 cut(s) 81, 645, 654
MslI CAYNNNNRTG 2 cut(s) 122, 518
Msp20I TGGCCA 2 cut(s) 140, 234
MspA1I CMGCKG 1 cut(s) 224
MspR9I CCNGG 2 cut(s) 387, 610
Mva1269I GAATGC 2 cut(s) 288, 628
MvaI CCWGG 2 cut(s) 387, 610
MwoI GCNNNNNNNGC 1 cut(s) 450
NdeII GATC 1 cut(s) 417
NlaIII CATG 1 cut(s) 182
NlaIV GGNNCC 3 cut(s) 373, 374, 607
NmuCI GTSAC 2 cut(s) 73, 199
OliI CACNNNNGTG 1 cut(s) 122
PceI AGGCCT 1 cut(s) 152
PctI GAATGC 2 cut(s) 288, 628
PflMI CCANNNNNTGG 1 cut(s) 91
PkrI GCNGC 2 cut(s) 27, 226
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PshBI ATTAAT 1 cut(s) 654
Psp6I CCWGG 2 cut(s) 385, 608
PspFI CCCAGC 2 cut(s) 220, 375
PspGI CCWGG 2 cut(s) 385, 608
PspN4I GGNNCC 3 cut(s) 373, 374, 607
PspOMI GGGCCC 1 cut(s) 372
PspPI GGNCC 3 cut(s) 372, 373, 606
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 2 cut(s) 122, 518
SaqAI TTAA 3 cut(s) 81, 645, 654
SatI GCNGC 2 cut(s) 26, 225
Sau3AI GATC 1 cut(s) 417
Sau96I GGNCC 3 cut(s) 372, 373, 606
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 387, 610
SduI GDGCHC 3 cut(s) 134, 243, 376
SetI ASST 6 cut(s) 93, 226, 381, 408, 427, 591
SinI GGWCC 1 cut(s) 606
SmiMI CAYNNNNRTG 2 cut(s) 122, 518
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 5 cut(s) 54, 62, 192, 684, 701
SseBI AGGCCT 1 cut(s) 152
SspMI CTAG 2 cut(s) 477, 724
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 385, 608
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 3 cut(s) 136, 694, 709
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 420, 575
TaqII GACCGA 1 cut(s) 230
TasI AATT 5 cut(s) 54, 62, 192, 684, 701
TatI WGTACW 1 cut(s) 615
Tru1I TTAA 3 cut(s) 81, 645, 654
Tru9I TTAA 3 cut(s) 81, 645, 654
TscAI CASTG 5 cut(s) 141, 352, 420, 520, 697
TseFI GTSAC 2 cut(s) 73, 199
TseI GCWGC 2 cut(s) 25, 224
Tsp45I GTSAC 2 cut(s) 73, 199
TspRI CASTG 5 cut(s) 141, 352, 420, 520, 697
Van91I CCANNNNNTGG 1 cut(s) 91
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 606
VspI ATTAAT 1 cut(s) 654
XspI CTAG 2 cut(s) 477, 724
ZrmI AGTACT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.