FvH4_7g21010

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003115
Physical Location & Seq
Reverse (-)
16945073 .. 16945936
864 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21010.t1

Sequence Viewer

Length: 864 bp
ATGTTCTCTGTCACCTACCTACTATTCCTCACCCTCACCATTTTCTCTGCCATCTCAGCACGAACTCTCGAGGAGCAACCACCGGTTCCTGTAGTCCCTCCGGTGGTGTCTAATATAATCAGCACACCTTCCATCAATGCTGTACCAGTAGTCACTCCTTCAGCTACACAGCCTGCCACAGTGGCTGATGACCACCATTCCCTAGTCTTCTTCCTGCATGACATTCTAGGGGGCACAAACCCCACAGCTAGAGCCATGACAGGGATAGTCAATAGCCCTGCAGTCAATGGTCAAGTACCATTTGCCAAACCAAATGGAGCAGTCCTGCCATTTAACAATGGGGTACCCCAGAACAACAACAACAACGGTATTCTAAGCAACAACAACATCCCTTTTCTCACTGGACTCAGTGGAACTACCCAAAATGCGATACGAAACAATAACAATAACAACAACAACAATTTCATCAATGGTGGACAGCTCTTTCCAGGAGGCTCATCACTCCAAAACCTCATGTTTGGCACAATGAGTGTGTTTGATGATGAGCTAACCGAAGGGCATGAGCTGGGGTCTGGTTTGGTTGGCAAGGCACAAGGTTTCTATGTGGTGAGTTCTGAAGATGGTACTAGTCAGACTATGGCTTTCACTGCTATGTTTGAAAGTGGGGGTTATGCTGATAGTCTTAGCTTCTTTGGGGTCCATCGTGTGGCTGCTTCGGAGTCTCACTTGGCCATCATGGGTGGCACCGGAAAGTATGTTGATGCGAAAGGGTTTGCCATTGTTAAGACCTTCCCAGCTACCAATGAGCAACAAACTGATGGAGCTGAGACCTTGCTGCAGTTCATTGTTTATATTACTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

288

Amino Acids

30.3

Weight (kDa)

4.81

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 160 - 269 6.3e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 343
AccB1I GGYRCC 2 cut(s) 343, 743
AccB7I CCANNNNNTGG 1 cut(s) 706
AcoI YGGCCR 1 cut(s) 729
AcuI CTGAAG 2 cut(s) 144, 636
AfaI GTAC 4 cut(s) 144, 297, 345, 625
AfiI CCNNNNNNNGG 3 cut(s) 103, 261, 706
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 1 cut(s) 659
AhlI ACTAGT 1 cut(s) 626
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 8 cut(s) 164, 248, 481, 547, 565, 687, 797, 824
AluI AGCT 8 cut(s) 164, 248, 481, 547, 565, 687, 797, 824
Alw26I GTCTC 2 cut(s) 726, 821
AlwNI CAGNNNCTG 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 1 cut(s) 729
ApeKI GCWGC 2 cut(s) 710, 835
AsiGI ACCGGT 1 cut(s) 82
Asp718I GGTACC 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 697
AsuHPI GGTGA 4 cut(s) 4, 22, 28, 619
AvaI CYCGRG 1 cut(s) 68
AvaII GGWCC 1 cut(s) 697
BaeGI GKGCMC 1 cut(s) 236
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 2 cut(s) 343, 743
BbsI GAAGAC 1 cut(s) 199
BbvI GCAGC 2 cut(s) 697, 822
BccI CCATC 6 cut(s) 59, 140, 614, 708, 740, 812
BciT130I CCWGG 1 cut(s) 489
BcoDI GTCTC 2 cut(s) 726, 821
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 4 cut(s) 203, 227, 249, 627
BfmI CTRYAG 3 cut(s) 90, 279, 836
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 2 cut(s) 711, 836
BlsI GCNGC 2 cut(s) 712, 837
Bme1390I CCNGG 1 cut(s) 489
Bme18I GGWCC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 4 cut(s) 87, 345, 698, 745
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 751
BpiI GAAGAC 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 821
BsaWI WCCGGW 3 cut(s) 82, 100, 746
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 261, 706
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 146, 406
BseBI CCWGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 3 cut(s) 103, 261, 706
BseMII CTCAG 3 cut(s) 69, 421, 816
BseNI ACTGG 2 cut(s) 146, 406
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 2 cut(s) 697, 822
BseYI CCCAGC 2 cut(s) 565, 793
BshFI GGCC 1 cut(s) 731
BshNI GGYRCC 2 cut(s) 343, 743
BshTI ACCGGT 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 3 cut(s) 83, 101, 747
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 103, 261, 706
BsmAI GTCTC 2 cut(s) 726, 821
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 731
Bso31I GGTCTC 1 cut(s) 821
BsoBI CYCGRG 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 236
BspANI GGCC 1 cut(s) 731
BspCNI CTCAG 3 cut(s) 68, 420, 817
BspLI GGNNCC 4 cut(s) 87, 345, 698, 745
BspMAI CTGCAG 2 cut(s) 283, 840
BspT107I GGYRCC 2 cut(s) 343, 743
BspTNI GGTCTC 1 cut(s) 821
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 2 cut(s) 146, 406
BssAI RCCGGY 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 2 cut(s) 181, 368
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 5 cut(s) 55, 374, 407, 683, 825
BstF5I GGATG 1 cut(s) 387
BstMAI GTCTC 2 cut(s) 726, 821
BstMWI GCNNNNNNNGC 3 cut(s) 56, 182, 647
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 3 cut(s) 90, 279, 836
BstSLI GKGCMC 1 cut(s) 236
BstV1I GCAGC 2 cut(s) 697, 822
BstV2I GAAGAC 1 cut(s) 199
BsuRI GGCC 1 cut(s) 731
BtsCI GGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 4 cut(s) 186, 399, 415, 645
Cac8I GCNNGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 697
Csp6I GTAC 4 cut(s) 143, 296, 344, 624
CspAI ACCGGT 1 cut(s) 82
CviAII CATG 5 cut(s) 218, 256, 514, 560, 736
CviQI GTAC 4 cut(s) 143, 296, 344, 624
DdeI CTNAG 5 cut(s) 55, 374, 407, 683, 825
EaeI YGGCCR 1 cut(s) 729
Eco31I GGTCTC 1 cut(s) 821
Eco47I GGWCC 1 cut(s) 697
Eco57I CTGAAG 2 cut(s) 144, 636
Eco88I CYCGRG 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 487
FaeI CATG 5 cut(s) 221, 259, 517, 563, 739
FaqI GGGAC 1 cut(s) 80
FatI CATG 5 cut(s) 217, 255, 513, 559, 735
Fnu4HI GCNGC 2 cut(s) 711, 836
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 2 cut(s) 711, 836
FspBI CTAG 4 cut(s) 203, 227, 249, 627
GluI GCNGC 2 cut(s) 711, 836
GsaI CCCAGC 2 cut(s) 569, 797
HaeIII GGCC 1 cut(s) 731
HapII CCGG 3 cut(s) 83, 101, 747
Hin1II CATG 5 cut(s) 221, 259, 517, 563, 739
HinfI GANTC 2 cut(s) 405, 719
HpaII CCGG 3 cut(s) 83, 101, 747
HphI GGTGA 4 cut(s) 4, 22, 28, 619
Hpy166II GTNNAC 1 cut(s) 476
Hpy188I TCNGA 3 cut(s) 616, 633, 718
Hpy188III TCNNGA 1 cut(s) 68
Hpy8I GTNNAC 1 cut(s) 476
HpyAV CCTTC 4 cut(s) 138, 168, 548, 799
HpyCH4III ACNGT 2 cut(s) 181, 368
HpyCH4V TGCA 3 cut(s) 217, 281, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 182, 647
HpyF3I CTNAG 5 cut(s) 55, 374, 407, 683, 825
Hsp92II CATG 5 cut(s) 221, 259, 517, 563, 739
KpnI GGTACC 1 cut(s) 347
LmnI GCTCC 3 cut(s) 73, 317, 821
Lsp1109I GCAGC 2 cut(s) 697, 822
LweI GCATC 1 cut(s) 751
MaeI CTAG 4 cut(s) 203, 227, 249, 627
MaeIII GTNAC 2 cut(s) 10, 151
MboII GAAGA 3 cut(s) 199, 202, 629
MhlI GDGCHC 1 cut(s) 236
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 1 cut(s) 460
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 2 cut(s) 399, 728
MnlI CCTC 6 cut(s) 38, 44, 64, 108, 485, 521
Mox20I TGGCCA 1 cut(s) 731
MscI TGGCCA 1 cut(s) 731
MseI TTAA 2 cut(s) 333, 783
MslI CAYNNNNRTG 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 731
MspI CCGG 3 cut(s) 83, 101, 747
MspR9I CCNGG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 3 cut(s) 56, 182, 647
NlaIII CATG 5 cut(s) 221, 259, 517, 563, 739
NlaIV GGNNCC 4 cut(s) 87, 345, 698, 745
NmuCI GTSAC 2 cut(s) 10, 151
PaeR7I CTCGAG 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 706
PfoI TCCNGGA 1 cut(s) 487
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 2 cut(s) 712, 837
PleI GAGTC 2 cut(s) 399, 727
PpsI GAGTC 2 cut(s) 399, 727
Psp6I CCWGG 1 cut(s) 487
PspFI CCCAGC 2 cut(s) 565, 793
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 4 cut(s) 87, 345, 698, 745
PspPI GGNCC 1 cut(s) 697
PstI CTGCAG 2 cut(s) 283, 840
PstNI CAGNNNCTG 1 cut(s) 185
RsaI GTAC 4 cut(s) 144, 297, 345, 625
RsaNI GTAC 4 cut(s) 143, 296, 344, 624
RseI CAYNNNNRTG 1 cut(s) 650
SaqAI TTAA 2 cut(s) 333, 783
SatI GCNGC 2 cut(s) 711, 836
Sau96I GGNCC 1 cut(s) 697
SchI GAGTC 2 cut(s) 399, 728
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 1 cut(s) 236
SfaNI GCATC 1 cut(s) 751
SfcI CTRYAG 3 cut(s) 90, 279, 836
Sfr274I CTCGAG 1 cut(s) 68
SinI GGWCC 1 cut(s) 697
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SpeI ACTAGT 1 cut(s) 626
Sse9I AATT 1 cut(s) 460
SspMI CTAG 4 cut(s) 203, 227, 249, 627
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 2 cut(s) 181, 368
TaqI TCGA 1 cut(s) 69
TasI AATT 1 cut(s) 460
Tru1I TTAA 2 cut(s) 333, 783
Tru9I TTAA 2 cut(s) 333, 783
TscAI CASTG 4 cut(s) 186, 406, 415, 652
TseFI GTSAC 2 cut(s) 10, 151
TseI GCWGC 2 cut(s) 710, 835
Tsp45I GTSAC 2 cut(s) 10, 151
TspDTI ATGAA 2 cut(s) 454, 832
TspRI CASTG 4 cut(s) 186, 406, 415, 652
Van91I CCANNNNNTGG 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 68
XspI CTAG 4 cut(s) 203, 227, 249, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.