MD15G1209700.v1.1

NPR1/NIM1 like defence protein C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003115
Physical Location & Seq
Reverse (-)
16815109 .. 16815744
636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1209700.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGCATTACCCCACAACCCTCCCCCACCCGAATCAGCCCTCAACCGCCCCTCCGACAACCTCGAATCGATCTTCGACTCCACTCACTTTGGCTACTTCGTCGACGCCAATATCATCGTCAACACCACCTTGGAAGTTCCCGTCCACCGCTGCGCCTTGTCGGCGAGAAACTCGTTTTTCAAGAACGTGTTCTCGGTGAAGGACAGAGGATCATCGTTGGTGGCTACTCGGTTTGAATTAAAGGAGTTCGTGAAGGAATATGATGTCGGATTGGACACGCTCCTCACTGTTTTGGGGTATTTGTACAGCGGGAAGCTTAGGTCATTGCCCAAAGGCATTTGCGTCTGCGTGGACGACGCTTGCGCTCATGTCGCTCGCTGGTCGGTGGTTGATTTCATGGTGGAGATTCTGTATGCTTCTGCAACTTTTCAAATCTCCGAATGGGTCTCACTTTATCAGGTAAAAACTTCACTAGTATCTTTTTGGGTAAAGTACAAAAAACTACCTCAACTATTAGTGTCACGACACTTTCATACCTTATCTTTTAAAATTGACAATGTCATACCTCATCTTACGAATTTGATCCAATGTTATACCTTCCGTTACTTGGCACTGAATTTTTCAGTTGAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001666 GO:0002376 GO:0002682 GO:0002831 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009625 GO:0009627 GO:0009628 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010033 GO:0010112 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0014070 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031348 GO:0032101 GO:0032870 GO:0035690 GO:0036293 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048585 GO:0050776 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0080027 GO:0080090 GO:0080134 GO:0098542 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000022 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 BTB DUF3420 NPR1_like_C

Protein Analysis

212

Amino Acids

24.02

Weight (kDa)

7.64

Isoelectric Point (pI)

29.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 31 - 107 5.6e-06 BTB/POZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 102
AciI CCGC 3 cut(s) 46, 148, 309
AclWI GGATC 2 cut(s) 217, 577
AcsI RAATTY 2 cut(s) 577, 616
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 2 cut(s) 305, 494
AfiI CCNNNNNNNGG 1 cut(s) 607
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 4 cut(s) 181, 236, 431, 629
AhlI ACTAGT 1 cut(s) 472
AluBI AGCT 1 cut(s) 316
AluI AGCT 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 2 cut(s) 217, 577
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 2 cut(s) 577, 616
Asp700I GAANNNNTTC 1 cut(s) 188
AspLEI GCGC 2 cut(s) 155, 365
AsuHPI GGTGA 1 cut(s) 208
BbvI GCAGC 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 451
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 1 cut(s) 473
BglI GCCNNNNNGGC 1 cut(s) 161
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 317
Bsa29I ATCGAT 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 129
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 395, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse3DI GCAATG 1 cut(s) 323
BseCI ATCGAT 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMI GCAATG 1 cut(s) 323
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 1 cut(s) 137
BshVI ATCGAT 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 635
Bso31I GGTCTC 1 cut(s) 451
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 3 cut(s) 69, 209, 582
BspACI CCGC 3 cut(s) 46, 148, 309
BspDI ATCGAT 1 cut(s) 68
BspPI GGATC 2 cut(s) 217, 577
BspTNI GGTCTC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 3 cut(s) 69, 209, 582
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 105
BstAUI TGTACA 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 361, 376
BstDEI CTNAG 1 cut(s) 317
BstHHI GCGC 2 cut(s) 155, 365
BstKTI GATC 3 cut(s) 72, 212, 585
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 3 cut(s) 69, 209, 582
BstMWI GCNNNNNNNGC 2 cut(s) 161, 371
BstV1I GCAGC 1 cut(s) 137
Bsu15I ATCGAT 1 cut(s) 68
BsuTUI ATCGAT 1 cut(s) 68
BtsIMutI CAGTG 2 cut(s) 285, 611
Cac8I GCNNGC 2 cut(s) 361, 376
CfoI GCGC 2 cut(s) 155, 365
ClaI ATCGAT 1 cut(s) 68
CseI GACGC 3 cut(s) 113, 331, 365
Csp6I GTAC 2 cut(s) 304, 493
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
CviAII CATG 2 cut(s) 368, 397
CviJI RGCY 4 cut(s) 38, 93, 224, 316
CviKI_1 RGCY 4 cut(s) 38, 93, 224, 316
CviQI GTAC 2 cut(s) 304, 493
DdeI CTNAG 1 cut(s) 317
DpnI GATC 3 cut(s) 71, 211, 584
DpnII GATC 3 cut(s) 69, 209, 582
DraI TTTAAA 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 2 cut(s) 371, 400
FaiI YATR 7 cut(s) 261, 369, 398, 414, 534, 563, 594
FatI CATG 2 cut(s) 367, 396
FauI CCCGC 1 cut(s) 302
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 151
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 1 cut(s) 473
GlaI GCGC 2 cut(s) 154, 364
GluI GCNGC 1 cut(s) 151
HgaI GACGC 3 cut(s) 113, 331, 365
HhaI GCGC 2 cut(s) 155, 365
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 371, 400
Hin6I GCGC 2 cut(s) 153, 363
HinP1I GCGC 2 cut(s) 153, 363
HincII GTYRAC 2 cut(s) 103, 121
HindII GTYRAC 2 cut(s) 103, 121
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 4 cut(s) 32, 65, 77, 406
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 4 cut(s) 103, 121, 145, 352
Hpy188I TCNGA 3 cut(s) 55, 269, 439
Hpy188III TCNNGA 3 cut(s) 181, 250, 522
Hpy8I GTNNAC 4 cut(s) 103, 121, 145, 352
Hpy99I CGWCG 3 cut(s) 104, 107, 359
HpyAV CCTTC 3 cut(s) 193, 247, 607
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 371
HpyF3I CTNAG 1 cut(s) 317
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 371, 400
HspAI GCGC 2 cut(s) 153, 363
Kzo9I GATC 3 cut(s) 69, 209, 582
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 2 cut(s) 364, 443
Lsp1109I GCAGC 1 cut(s) 137
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 519, 602
MalI GATC 3 cut(s) 71, 211, 584
MboI GATC 3 cut(s) 69, 209, 582
MboII GAAGA 1 cut(s) 64
MluCI AATT 4 cut(s) 236, 549, 577, 616
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 78, 247
MnlI CCTC 8 cut(s) 30, 50, 61, 71, 200, 293, 516, 576
MroXI GAANNNNTTC 1 cut(s) 188
MseI TTAA 2 cut(s) 239, 546
MspA1I CMGCKG 2 cut(s) 150, 309
Mva1269I GAATGC 1 cut(s) 635
MwoI GCNNNNNNNGC 2 cut(s) 161, 371
NdeII GATC 3 cut(s) 69, 209, 582
NlaIII CATG 2 cut(s) 371, 400
NmuCI GTSAC 1 cut(s) 519
PctI GAATGC 1 cut(s) 635
PdmI GAANNNNTTC 1 cut(s) 188
PfeI GAWTC 3 cut(s) 32, 65, 406
PflFI GACNNNGTC 1 cut(s) 557
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PsyI GACNNNGTC 1 cut(s) 557
RsaI GTAC 2 cut(s) 305, 494
RsaNI GTAC 2 cut(s) 304, 493
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 2 cut(s) 239, 546
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 3 cut(s) 69, 209, 582
SchI GAGTC 1 cut(s) 71
SgrDI CGTCGACG 1 cut(s) 101
SpeI ACTAGT 1 cut(s) 472
Sse9I AATT 4 cut(s) 236, 549, 577, 616
SsiI CCGC 3 cut(s) 46, 148, 309
SspMI CTAG 1 cut(s) 473
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 189
TaqI TCGA 4 cut(s) 63, 68, 75, 102
TasI AATT 4 cut(s) 236, 549, 577, 616
TatI WGTACW 2 cut(s) 303, 492
TfiI GAWTC 3 cut(s) 32, 65, 406
Tru1I TTAA 2 cut(s) 239, 546
Tru9I TTAA 2 cut(s) 239, 546
TscAI CASTG 2 cut(s) 292, 618
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 2 cut(s) 385, 521
TspGWI ACGGA 1 cut(s) 590
TspRI CASTG 2 cut(s) 292, 618
Tth111I GACNNNGTC 1 cut(s) 557
XapI RAATTY 2 cut(s) 577, 616
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 188
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.