Rw2G025450

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003115
Physical Location & Seq
Forward (+)
38007935 .. 38008864
930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025450.1

Sequence Viewer

Length: 930 bp
ATGGCAACCCAAAACTCAACTCCGCTGCCCCTCAAGGCCATAACATACCTATTCCTTCTAGCTCTTGCAATAACACACACCACCTCAGCCAGAGTCCTTGATGAGGAAACCGATACGCCTCAAACCCAAATACCTGTTTCCACCGTACCTCCTTCTGTGGTCGGTGCCACGTCAGCGGGCCCTCCTCCTGTTGTCAGTGCCACGTCAGCAGGCCCGAGCGCCACAGTGGCCAATGGAGAGTCCTACCATCCGCTTTCATTCTTTCTGCATGATATAATTGGTGGCACCAATCCCTCAGCGAGAGCTGTGACTGGGATTGTGACCAACCCAGCTGTCAATGGCCAAGTCCCCTTTGCCAAACCCAATGGGGCAGTTGTCCAAGGCGGGAATAATGGCATTCCCCAAAACAACAACAACAATGGTATCATCAACAATAACAATGTCCCCTTCTTCACTGGCCTCGGAGGAAACATCAACAACAACCTCAGACAAAACAACAACAACTTTATTGGCGGGAACAATGGGTTCCCCGTTGTGAATGGGGCGCAGCTCCCGCCAGGACAGTCCCTTCAGAAGCTCATGTTCGGCACACTCACGGTCTTTGACGATGAGCTCACTGAAGGGCATGAGCTTGGCTCTGGCTTGTTGGGCAAGGCACAAGGATTTTACGTTGCTAGTTCCGAAGATGGGAGCAGCCAGACCATTGCTTTCACTGCTATGTTTGAGAGTGGTGGGTATGCTGATAGCCTCACATTCTTTGGGGTTCACAGAATAGCTGCTTCAGAGTCTCACCTTGCAGTTATGGGAGGGACAGGGAAGTACTTGAATGCAAAAGGGTATGCTTTGGTTAAGACCATTAATGGAGTGAATGAGCAAAATACTGATGGAATTGACACTGTTCTTCAGTTTACTGTATATCTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

309

Amino Acids

32.04

Weight (kDa)

5.08

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 170 - 307 8.4e-29 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 164, 284
AciI CCGC 6 cut(s) 23, 176, 251, 384, 513, 554
AcoI YGGCCR 2 cut(s) 228, 340
AcuI CTGAAG 4 cut(s) 554, 639, 765, 887
AfaI GTAC 2 cut(s) 147, 821
AfiI CCNNNNNNNGG 2 cut(s) 103, 687
AgsI TTSAA 1 cut(s) 826
AjiI CACGTC 2 cut(s) 171, 204
AjnI CCWGG 1 cut(s) 556
AluBI AGCT 8 cut(s) 62, 305, 332, 550, 577, 613, 631, 776
AluI AGCT 8 cut(s) 62, 305, 332, 550, 577, 613, 631, 776
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 1 cut(s) 792
Ama87I CYCGRG 1 cut(s) 214
AoxI GGCC 6 cut(s) 36, 178, 211, 228, 340, 457
ApaI GGGCCC 1 cut(s) 182
ApeKI GCWGC 4 cut(s) 25, 547, 693, 776
AseI ATTAAT 1 cut(s) 858
AspLEI GCGC 2 cut(s) 221, 547
AspS9I GGNCC 3 cut(s) 178, 179, 212
AsuHPI GGTGA 1 cut(s) 782
AvaI CYCGRG 1 cut(s) 214
BaeGI GKGCMC 1 cut(s) 182
BaeI ACNNNNGTAYC 2 cut(s) 406, 439
BalI TGGCCA 2 cut(s) 230, 342
BanI GGYRCC 2 cut(s) 164, 284
BanII GRGCYC 2 cut(s) 182, 615
Bbv12I GWGCWC 1 cut(s) 615
BbvCI CCTCAGC 2 cut(s) 85, 295
BbvI GCAGC 4 cut(s) 12, 559, 705, 763
BccI CCATC 3 cut(s) 255, 680, 878
BciT130I CCWGG 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 3 cut(s) 59, 675, 928
BfoI RGCGCY 1 cut(s) 222
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 4 cut(s) 26, 548, 694, 777
BlsI GCNGC 4 cut(s) 27, 549, 695, 778
BmcAI AGTACT 1 cut(s) 821
Bme1390I CCNGG 1 cut(s) 558
BmeT110I CYCGRG 1 cut(s) 214
BmgBI CACGTC 2 cut(s) 171, 204
BmgT120I GGNCC 3 cut(s) 178, 179, 212
BmiI GGNNCC 4 cut(s) 166, 180, 286, 527
BmrFI CCNGG 1 cut(s) 558
BmrI ACTGGG 1 cut(s) 321
BmuI ACTGGG 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 620, 652
Bpu10I CCTNAGC 2 cut(s) 85, 295
BpuEI CTTGAG 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 379, 460
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 687
Bse1I ACTGG 2 cut(s) 316, 460
Bse3DI GCAATG 1 cut(s) 702
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 2 cut(s) 379, 460
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 103, 687
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 3 cut(s) 99, 309, 499
BseNI ACTGG 2 cut(s) 316, 460
BseRI GAGGAG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 182
BseXI GCAGC 4 cut(s) 12, 559, 705, 763
BseYI CCCAGC 1 cut(s) 328
BshFI GGCC 6 cut(s) 38, 180, 213, 230, 342, 459
BshNI GGYRCC 2 cut(s) 164, 284
BsiHKAI GWGCWC 1 cut(s) 615
BsiHKCI CYCGRG 1 cut(s) 214
BslFI GGGAC 4 cut(s) 332, 428, 550, 823
BslI CCNNNNNNNGG 2 cut(s) 103, 687
BsmAI GTCTC 1 cut(s) 792
BsmFI GGGAC 4 cut(s) 332, 428, 550, 823
BsmI GAATGC 2 cut(s) 396, 832
BsnI GGCC 6 cut(s) 38, 180, 213, 230, 342, 459
BsoBI CYCGRG 1 cut(s) 214
Bsp120I GGGCCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 182, 615
BspACI CCGC 6 cut(s) 23, 176, 251, 384, 513, 554
BspANI GGCC 6 cut(s) 38, 180, 213, 230, 342, 459
BspCNI CTCAG 3 cut(s) 98, 308, 498
BspLI GGNNCC 4 cut(s) 166, 180, 286, 527
BspT107I GGYRCC 2 cut(s) 164, 284
BsrDI GCAATG 1 cut(s) 702
BsrI ACTGG 2 cut(s) 316, 460
BssECI CCNNGG 2 cut(s) 379, 460
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 6 cut(s) 145, 226, 564, 598, 898, 913
BstC8I GCNNGC 2 cut(s) 178, 211
BstDEI CTNAG 4 cut(s) 85, 295, 485, 920
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 547
BstMAI GTCTC 1 cut(s) 792
BstMWI GCNNNNNNNGC 6 cut(s) 173, 206, 227, 553, 648, 713
BstNI CCWGG 1 cut(s) 558
BstSCI CCNGG 1 cut(s) 556
BstSLI GKGCMC 1 cut(s) 182
BstV1I GCAGC 4 cut(s) 12, 559, 705, 763
BsuRI GGCC 6 cut(s) 38, 180, 213, 230, 342, 459
BtrI CACGTC 2 cut(s) 171, 204
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 6 cut(s) 202, 231, 453, 615, 711, 894
Cac8I GCNNGC 2 cut(s) 178, 211
CfoI GCGC 2 cut(s) 221, 547
Cfr13I GGNCC 3 cut(s) 178, 179, 212
Csp6I GTAC 2 cut(s) 146, 820
CviAII CATG 3 cut(s) 269, 580, 626
CviQI GTAC 2 cut(s) 146, 820
DdeI CTNAG 4 cut(s) 85, 295, 485, 920
EaeI YGGCCR 2 cut(s) 228, 340
Ecl136II GAGCTC 1 cut(s) 613
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 2 cut(s) 182, 615
Eco53kI GAGCTC 1 cut(s) 613
Eco57I CTGAAG 4 cut(s) 554, 639, 765, 887
Eco88I CYCGRG 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 613
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 379
EcoT38I GRGCYC 2 cut(s) 182, 615
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 3 cut(s) 272, 583, 629
FaqI GGGAC 4 cut(s) 332, 428, 550, 823
FatI CATG 3 cut(s) 268, 579, 625
FauI CCCGC 4 cut(s) 169, 377, 506, 561
Fnu4HI GCNGC 4 cut(s) 26, 548, 694, 777
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 2 cut(s) 182, 615
Fsp4HI GCNGC 4 cut(s) 26, 548, 694, 777
FspBI CTAG 3 cut(s) 59, 675, 928
GlaI GCGC 2 cut(s) 220, 546
GluI GCNGC 4 cut(s) 26, 548, 694, 777
GsaI CCCAGC 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 6 cut(s) 38, 180, 213, 230, 342, 459
HhaI GCGC 2 cut(s) 221, 547
Hin1II CATG 3 cut(s) 272, 583, 629
Hin6I GCGC 2 cut(s) 219, 545
HinP1I GCGC 2 cut(s) 219, 545
HinfI GANTC 3 cut(s) 93, 239, 785
HphI GGTGA 1 cut(s) 782
Hpy166II GTNNAC 2 cut(s) 766, 909
Hpy188I TCNGA 5 cut(s) 464, 488, 573, 682, 784
Hpy8I GTNNAC 2 cut(s) 766, 909
HpyAV CCTTC 5 cut(s) 65, 162, 457, 578, 614
HpyCH4III ACNGT 6 cut(s) 145, 226, 564, 598, 898, 913
HpyCH4IV ACGT 3 cut(s) 170, 203, 669
HpyCH4V TGCA 4 cut(s) 68, 268, 797, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 206, 227, 553, 648, 713
HpyF3I CTNAG 4 cut(s) 85, 295, 485, 920
HpySE526I ACGT 3 cut(s) 170, 203, 669
Hsp92II CATG 3 cut(s) 272, 583, 629
HspAI GCGC 2 cut(s) 219, 545
LmnI GCTCC 2 cut(s) 555, 690
Lsp1109I GCAGC 4 cut(s) 12, 559, 705, 763
MaeI CTAG 3 cut(s) 59, 675, 928
MaeII ACGT 3 cut(s) 170, 203, 669
MaeIII GTNAC 3 cut(s) 307, 319, 923
MboII GAAGA 3 cut(s) 442, 695, 893
MhlI GDGCHC 2 cut(s) 182, 615
MlsI TGGCCA 2 cut(s) 230, 342
MluCI AATT 2 cut(s) 276, 888
MluNI TGGCCA 2 cut(s) 230, 342
MlyI GAGTC 3 cut(s) 102, 248, 794
Mox20I TGGCCA 2 cut(s) 230, 342
MscI TGGCCA 2 cut(s) 230, 342
MseI TTAA 2 cut(s) 849, 858
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 2 cut(s) 230, 342
MspA1I CMGCKG 3 cut(s) 25, 176, 332
MspR9I CCNGG 1 cut(s) 558
Mva1269I GAATGC 2 cut(s) 396, 832
MvaI CCWGG 1 cut(s) 558
MwoI GCNNNNNNNGC 6 cut(s) 173, 206, 227, 553, 648, 713
NlaIII CATG 3 cut(s) 272, 583, 629
NlaIV GGNNCC 4 cut(s) 166, 180, 286, 527
NmuCI GTSAC 2 cut(s) 307, 319
PctI GAATGC 2 cut(s) 396, 832
PkrI GCNGC 4 cut(s) 27, 549, 695, 778
PleI GAGTC 3 cut(s) 101, 247, 793
PpsI GAGTC 3 cut(s) 101, 247, 793
PshBI ATTAAT 1 cut(s) 858
Psp124BI GAGCTC 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 556
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 4 cut(s) 166, 180, 286, 527
PspOMI GGGCCC 1 cut(s) 178
PspPI GGNCC 3 cut(s) 178, 179, 212
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 147, 821
RsaNI GTAC 2 cut(s) 146, 820
RseI CAYNNNNRTG 1 cut(s) 716
SacI GAGCTC 1 cut(s) 615
SaqAI TTAA 2 cut(s) 849, 858
SatI GCNGC 4 cut(s) 26, 548, 694, 777
Sau96I GGNCC 3 cut(s) 178, 179, 212
ScaI AGTACT 1 cut(s) 821
SchI GAGTC 3 cut(s) 102, 248, 794
ScrFI CCNGG 1 cut(s) 558
SduI GDGCHC 2 cut(s) 182, 615
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 2 cut(s) 276, 888
SsiI CCGC 6 cut(s) 23, 176, 251, 384, 513, 554
SspMI CTAG 3 cut(s) 59, 675, 928
SstI GAGCTC 1 cut(s) 615
StyD4I CCNGG 1 cut(s) 556
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 6 cut(s) 145, 226, 564, 598, 898, 913
TaiI ACGT 3 cut(s) 173, 206, 672
TasI AATT 2 cut(s) 276, 888
TatI WGTACW 1 cut(s) 819
Tru1I TTAA 2 cut(s) 849, 858
Tru9I TTAA 2 cut(s) 849, 858
TscAI CASTG 6 cut(s) 202, 231, 460, 622, 718, 901
TseFI GTSAC 2 cut(s) 307, 319
TseI GCWGC 4 cut(s) 25, 547, 693, 776
Tsp45I GTSAC 2 cut(s) 307, 319
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 6 cut(s) 202, 231, 460, 622, 718, 901
VspI ATTAAT 1 cut(s) 858
XagI CCTNNNNNAGG 1 cut(s) 101
XspI CTAG 3 cut(s) 59, 675, 928
ZrmI AGTACT 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.