Rh1CG308600

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003115
Physical Location & Seq
Reverse (-)
58267031 .. 58268069
1039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG308600.1

Sequence Viewer

Length: 864 bp
ATGTTCTCCGTCACCCACCTACTATTCCTCACCCTCACCATTTTATCTGCCACCTCAGCGCGAACTCTCGAGGAGCAGCCACTGGTTCCTGTTGTGCCTCCGGTGGTGTCGAATACAATTGGCATACCTTCCATCAATGCTGCACCAGTGGTCAGACCTTCAGCTTCACAGCCTGCCACGGTGGCTGATGCTGATGACCACCATTCCTTAGTCTTCTTCCTGCACGACATTCTAGGTGGCACGAACCCCTCAGCTAGAGCCATGACAGGGATAGTCAACAGCCCTGCAGTCAATGGACAAGTACCATTTGCCAAACCAAATGGAGCAGTCCTGCCATTTAACAATGGAATACCCCAGAACAACAACAACAACGGTATTCTAAGCAACAACAACATCCCGTTTCTCACTGGACTTAGTGGAGCAACGCAAAATGTGGTACAAAACAATAACAACAACAACAATTTTATCAATGGTGGGCAGCTCTTTCCAGGAGGCTCCACACTCCAAAACCTCATGTTCGGCACAATGAGTGTGTTCGATGATGAGCTAACTGAAGGGCATGAGCTCGGGTCTGGTTTGGTTGGCAAGGCACAAGGGTTCTATGTGGTGAGTTCTGAAGATGGTACGAGTCAGACTATGGCTTTCACTGCTATGTTTCAAAGTGGGGGTTATGCTGATAGCCTTAGCTTCTTTGGGGTCCATCGTGTGGCTGCGTCAGAGTCTCACTTGGCTATCATGGGTGGCACCGGAAAGTATGTTGATGCGAAAGGGTTTGCCATTGTTAAGACATTCCCGGCTACCAATGAGCATCAAACTGATGGAGCAGAGACCTTGTTGCAGTTCACTGTTTATATTACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

287

Amino Acids

30.15

Weight (kDa)

5.04

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 148 - 285 3.1e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 743
AccB7I CCANNNNNTGG 1 cut(s) 706
AccII CGCG 1 cut(s) 61
AcuI CTGAAG 3 cut(s) 144, 573, 636
AfaI GTAC 3 cut(s) 303, 438, 625
AfiI CCNNNNNNNGG 2 cut(s) 267, 706
AgsI TTSAA 1 cut(s) 659
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 6 cut(s) 164, 254, 481, 547, 565, 687
AluI AGCT 6 cut(s) 164, 254, 481, 547, 565, 687
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 2 cut(s) 726, 821
AlwNI CAGNNNCTG 1 cut(s) 82
Ama87I CYCGRG 2 cut(s) 68, 566
ApeKI GCWGC 4 cut(s) 76, 140, 478, 710
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 697
AsuC2I CCSGG 1 cut(s) 794
AsuHPI GGTGA 4 cut(s) 4, 22, 28, 619
AvaI CYCGRG 2 cut(s) 68, 566
AvaII GGWCC 1 cut(s) 697
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 1 cut(s) 567
BbsI GAAGAC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 567
BbvCI CCTCAGC 2 cut(s) 55, 250
BbvI GCAGC 4 cut(s) 88, 127, 490, 697
BccI CCATC 4 cut(s) 140, 614, 708, 812
BciT130I CCWGG 1 cut(s) 489
BcnI CCSGG 1 cut(s) 794
BcoDI GTCTC 2 cut(s) 726, 821
BfaI CTAG 3 cut(s) 233, 255, 862
BfmI CTRYAG 1 cut(s) 285
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 4 cut(s) 77, 141, 479, 711
BlsI GCNGC 4 cut(s) 78, 142, 480, 712
Bme1390I CCNGG 2 cut(s) 489, 794
Bme18I GGWCC 1 cut(s) 697
BmeT110I CYCGRG 2 cut(s) 68, 566
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 4 cut(s) 87, 496, 698, 745
BmrFI CCNGG 2 cut(s) 489, 794
BmsI GCATC 3 cut(s) 178, 751, 817
BpiI GAAGAC 1 cut(s) 205
Bpu10I CCTNAGC 3 cut(s) 55, 250, 683
BpuMI CCSGG 1 cut(s) 794
BsaI GGTCTC 1 cut(s) 821
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 2 cut(s) 100, 746
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 706
Bse1I ACTGG 3 cut(s) 87, 146, 412
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 267, 706
BseMII CTCAG 2 cut(s) 69, 264
BseNI ACTGG 3 cut(s) 87, 146, 412
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 4 cut(s) 88, 127, 490, 697
BsgI GTGCAG 2 cut(s) 126, 206
Bsh1236I CGCG 1 cut(s) 61
BshNI GGYRCC 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 567
BsiHKCI CYCGRG 2 cut(s) 68, 566
BsiSI CCGG 3 cut(s) 101, 747, 794
BslI CCNNNNNNNGG 2 cut(s) 267, 706
BsmAI GTCTC 2 cut(s) 726, 821
Bso31I GGTCTC 1 cut(s) 821
BsoBI CYCGRG 2 cut(s) 68, 566
Bsp1286I GDGCHC 1 cut(s) 567
BspCNI CTCAG 2 cut(s) 68, 263
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 4 cut(s) 87, 496, 698, 745
BspMAI CTGCAG 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 743
BspTNI GGTCTC 1 cut(s) 821
BsrI ACTGG 3 cut(s) 87, 146, 412
BssECI CCNNGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 3 cut(s) 181, 374, 847
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 6 cut(s) 55, 208, 250, 380, 413, 683
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 1 cut(s) 61
BstMAI GTCTC 2 cut(s) 726, 821
BstMWI GCNNNNNNNGC 3 cut(s) 56, 182, 647
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 2 cut(s) 487, 792
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 4 cut(s) 88, 127, 490, 697
BstV2I GAAGAC 1 cut(s) 205
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 393
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 5 cut(s) 80, 153, 405, 645, 843
Cac8I GCNNGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 697
CseI GACGC 1 cut(s) 702
Csp6I GTAC 3 cut(s) 302, 437, 624
CviAII CATG 4 cut(s) 262, 514, 560, 736
CviQI GTAC 3 cut(s) 302, 437, 624
DdeI CTNAG 6 cut(s) 55, 208, 250, 380, 413, 683
Ecl136II GAGCTC 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 821
Eco47I GGWCC 1 cut(s) 697
Eco53kI GAGCTC 1 cut(s) 565
Eco57I CTGAAG 3 cut(s) 144, 573, 636
Eco88I CYCGRG 2 cut(s) 68, 566
EcoICRI GAGCTC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 567
FaeI CATG 4 cut(s) 265, 517, 563, 739
FatI CATG 4 cut(s) 261, 513, 559, 735
Fnu4HI GCNGC 4 cut(s) 77, 141, 479, 711
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 4 cut(s) 77, 141, 479, 711
FspBI CTAG 3 cut(s) 233, 255, 862
GlaI GCGC 1 cut(s) 60
GluI GCNGC 4 cut(s) 77, 141, 479, 711
HapII CCGG 3 cut(s) 101, 747, 794
HgaI GACGC 1 cut(s) 702
HhaI GCGC 1 cut(s) 61
Hin1II CATG 4 cut(s) 265, 517, 563, 739
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 2 cut(s) 628, 719
HpaII CCGG 3 cut(s) 101, 747, 794
HphI GGTGA 4 cut(s) 4, 22, 28, 619
Hpy166II GTNNAC 2 cut(s) 277, 843
Hpy188I TCNGA 4 cut(s) 155, 616, 633, 718
Hpy188III TCNNGA 1 cut(s) 68
Hpy8I GTNNAC 2 cut(s) 277, 843
HpyAV CCTTC 3 cut(s) 138, 168, 548
HpyCH4III ACNGT 3 cut(s) 181, 374, 847
HpyCH4V TGCA 4 cut(s) 143, 223, 287, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 182, 647
HpyF3I CTNAG 6 cut(s) 55, 208, 250, 380, 413, 683
Hsp92II CATG 4 cut(s) 265, 517, 563, 739
HspAI GCGC 1 cut(s) 59
LmnI GCTCC 5 cut(s) 73, 323, 419, 500, 821
Lsp1109I GCAGC 4 cut(s) 88, 127, 490, 697
LweI GCATC 3 cut(s) 178, 751, 817
MaeI CTAG 3 cut(s) 233, 255, 862
MaeIII GTNAC 1 cut(s) 10
MboII GAAGA 3 cut(s) 205, 208, 629
MfeI CAATTG 1 cut(s) 117
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 2 cut(s) 117, 460
MlyI GAGTC 2 cut(s) 637, 728
MnlI CCTC 8 cut(s) 38, 44, 64, 64, 108, 259, 485, 521
MseI TTAA 2 cut(s) 339, 783
MslI CAYNNNNRTG 1 cut(s) 650
MspI CCGG 3 cut(s) 101, 747, 794
MspR9I CCNGG 2 cut(s) 489, 794
MunI CAATTG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 489
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 3 cut(s) 56, 182, 647
NciI CCSGG 1 cut(s) 794
NlaIII CATG 4 cut(s) 265, 517, 563, 739
NlaIV GGNNCC 4 cut(s) 87, 496, 698, 745
NmuCI GTSAC 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 706
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 4 cut(s) 78, 142, 480, 712
PleI GAGTC 2 cut(s) 636, 727
PpsI GAGTC 2 cut(s) 636, 727
Psp124BI GAGCTC 1 cut(s) 567
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 4 cut(s) 87, 496, 698, 745
PspPI GGNCC 1 cut(s) 697
PstI CTGCAG 1 cut(s) 289
PstNI CAGNNNCTG 1 cut(s) 82
RsaI GTAC 3 cut(s) 303, 438, 625
RsaNI GTAC 3 cut(s) 302, 437, 624
RseI CAYNNNNRTG 1 cut(s) 650
SacI GAGCTC 1 cut(s) 567
SaqAI TTAA 2 cut(s) 339, 783
SatI GCNGC 4 cut(s) 77, 141, 479, 711
Sau96I GGNCC 1 cut(s) 697
SchI GAGTC 2 cut(s) 637, 728
ScrFI CCNGG 2 cut(s) 489, 794
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 3 cut(s) 178, 751, 817
SfcI CTRYAG 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 68
SinI GGWCC 1 cut(s) 697
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 2 cut(s) 117, 460
SspMI CTAG 3 cut(s) 233, 255, 862
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 487, 792
TaaI ACNGT 3 cut(s) 181, 374, 847
TaqI TCGA 3 cut(s) 69, 110, 537
TasI AATT 2 cut(s) 117, 460
Tru1I TTAA 2 cut(s) 339, 783
Tru9I TTAA 2 cut(s) 339, 783
TscAI CASTG 5 cut(s) 87, 153, 412, 652, 850
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 4 cut(s) 76, 140, 478, 710
Tsp45I GTSAC 1 cut(s) 10
TspRI CASTG 5 cut(s) 87, 153, 412, 652, 850
Van91I CCANNNNNTGG 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 68
XspI CTAG 3 cut(s) 233, 255, 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.