Rroxscaffold_2G00118620

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003115
Physical Location & Seq
Reverse (-)
50161532 .. 50165182
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118620.1

Sequence Viewer

Length: 1062 bp
ATGGTGACGTTCAACACCGGGAGCTGCAGGCTTGGAGATACTACCCAACAGTTAAGCTTGCCGCCGCCGCTACGGCCGCGCCGCCGTGTGCAAAGGCTGCCACGACAGGTGCTCAAGGACCATACGAAGCTATGGAAGAAGTTTGGTGAGGATTGGTGGCCGAAGGAGGAAGATATGGCTTGGAGAAGATTTCGGGCGATTTCCGGCGGGGAGCCGAGCTGCAAGTCCCTGACGAGGGAAACCGATACGCCTCAAACCCAAATACCTGTTTCCACCGTACCTCCTTCTGTGGTCGGTGCCACGTCAGCGGGCCCTCCTCCTGTGGTTGGTGCCACATCAGCAGGGCCGAGCGCCATAGTGGCCAATGGAGAGTCCTACCATCCGCTTTCATTCTTTCTGCATGATATAATTGGTGGCACCAATCCCTCAGCGAGAGCTGTGACTGGGATTGTAACCAACCCAGCTGTCAATGGCCAAGTCCCCTTTGCCAAACCCAATGGGGCAGTTGTCCAAGGTGTGAATAATGGCATTCCCCAAAACAACAACAACAATGGTATCATCAACAATAACAATGTCCCCTTCTTCACTGGCCTCGGAGGAAACATCAACAACAACCTCAGACAAAACAACAACTTTATTGGCGGGAACAATGGGTTCCCCGTTGTGAATGGGGCCCAGCTCCCGCCAGGACAGTCCCTTCAGAAGCTCATGTTCGGTACACTCACGGTCTTTGACGATGAGCTCACTGAAGGGCATGAGCTTGGCTCTGGCTTGTTGGGCAAGGCACAAGGATTTTACGTTGCTAGTTCCGAAGATGGGAGCAGCCAGACCATCGCTTTCACTGCAATGTTTGAGAGTGGTGGGTATGCTGATAGCCTCACATTCTTTGGGGTTCACAGAGTAGCTGCTTCAGAGTCTCACCTTGCAGTTATGGGAGGGACCGGGAAGTACTTGAATGCAAAAGGGTATGCTTTGGTTAAGACCATTAATGGAGTGAATGAGCAAAATACTGATGGAATTGACACTGTTCTTCTTCAGTTTACTGTGTATCTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

353

Amino Acids

37.6

Weight (kDa)

7.8

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 219 - 350 3.3e-28 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 296, 329, 416
AccII CGCG 1 cut(s) 79
AcoI YGGCCR 4 cut(s) 74, 158, 360, 472
AcuI CTGAAG 4 cut(s) 683, 768, 894, 1019
AfaI GTAC 3 cut(s) 279, 718, 950
AfiI CCNNNNNNNGG 4 cut(s) 234, 235, 326, 816
AgsI TTSAA 2 cut(s) 13, 955
AjiI CACGTC 1 cut(s) 303
AjnI CCWGG 1 cut(s) 685
Alw21I GWGCWC 2 cut(s) 114, 744
Alw26I GTCTC 1 cut(s) 921
AoxI GGCC 8 cut(s) 74, 158, 310, 344, 360, 472, 589, 672
ApaI GGGCCC 2 cut(s) 314, 676
ApeKI GCWGC 5 cut(s) 24, 97, 219, 822, 905
AseI ATTAAT 1 cut(s) 987
AspLEI GCGC 2 cut(s) 81, 353
AspS9I GGNCC 7 cut(s) 118, 310, 311, 344, 672, 673, 939
AsuC2I CCSGG 2 cut(s) 19, 943
AsuHPI GGTGA 3 cut(s) 16, 158, 911
AvaII GGWCC 2 cut(s) 118, 939
BaeGI GKGCMC 2 cut(s) 314, 676
BaeI ACNNNNGTAYC 2 cut(s) 538, 571
BalI TGGCCA 2 cut(s) 362, 474
BanI GGYRCC 3 cut(s) 296, 329, 416
BanII GRGCYC 3 cut(s) 314, 676, 744
Bbv12I GWGCWC 2 cut(s) 114, 744
BbvCI CCTCAGC 1 cut(s) 427
BbvI GCAGC 5 cut(s) 11, 84, 206, 834, 892
BccI CCATC 4 cut(s) 387, 809, 839, 1007
BceAI ACGGC 2 cut(s) 69, 89
BciT130I CCWGG 1 cut(s) 687
BcnI CCSGG 2 cut(s) 19, 943
BcoDI GTCTC 1 cut(s) 921
BfaI CTAG 2 cut(s) 804, 1060
BfmI CTRYAG 1 cut(s) 25
BfoI RGCGCY 1 cut(s) 354
BglI GCCNNNNNGGC 2 cut(s) 73, 359
BmcAI AGTACT 1 cut(s) 950
Bme1390I CCNGG 3 cut(s) 19, 687, 943
Bme18I GGWCC 2 cut(s) 118, 939
BmgBI CACGTC 1 cut(s) 303
BmgT120I GGNCC 7 cut(s) 118, 310, 311, 344, 672, 673, 939
BmiI GGNNCC 9 cut(s) 213, 298, 312, 331, 418, 656, 673, 674, 940
BmrFI CCNGG 3 cut(s) 19, 687, 943
BmrI ACTGGG 1 cut(s) 453
BmuI ACTGGG 1 cut(s) 453
BplI GAGNNNNNCTC 2 cut(s) 749, 781
Bpu10I CCTNAGC 1 cut(s) 427
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 2 cut(s) 19, 943
BsaJI CCNNGG 2 cut(s) 511, 592
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 235, 326, 816
Bse1I ACTGG 2 cut(s) 448, 592
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 1 cut(s) 687
BseDI CCNNGG 2 cut(s) 511, 592
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 4 cut(s) 234, 235, 326, 816
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 2 cut(s) 441, 631
BseNI ACTGG 2 cut(s) 448, 592
BseRI GAGGAG 1 cut(s) 306
BseSI GKGCMC 2 cut(s) 314, 676
BseX3I CGGCCG 1 cut(s) 74
BseXI GCAGC 5 cut(s) 11, 84, 206, 834, 892
BseYI CCCAGC 2 cut(s) 460, 675
Bsh1236I CGCG 1 cut(s) 79
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 8 cut(s) 76, 160, 312, 346, 362, 474, 591, 674
BshNI GGYRCC 3 cut(s) 296, 329, 416
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 2 cut(s) 114, 744
BsiSI CCGG 3 cut(s) 18, 204, 942
BslFI GGGAC 5 cut(s) 211, 464, 560, 679, 952
BslI CCNNNNNNNGG 4 cut(s) 234, 235, 326, 816
BsmAI GTCTC 1 cut(s) 921
BsmFI GGGAC 5 cut(s) 211, 464, 560, 679, 952
BsmI GAATGC 2 cut(s) 528, 961
BsnI GGCC 8 cut(s) 76, 160, 312, 346, 362, 474, 591, 674
Bsp120I GGGCCC 2 cut(s) 310, 672
Bsp1286I GDGCHC 4 cut(s) 114, 314, 676, 744
BspANI GGCC 8 cut(s) 76, 160, 312, 346, 362, 474, 591, 674
BspCNI CTCAG 2 cut(s) 440, 630
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 9 cut(s) 213, 298, 312, 331, 418, 656, 673, 674, 940
BspMAI CTGCAG 1 cut(s) 29
BspT107I GGYRCC 3 cut(s) 296, 329, 416
BsrDI GCAATG 1 cut(s) 852
BsrI ACTGG 2 cut(s) 448, 592
BssECI CCNNGG 2 cut(s) 511, 592
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 6 cut(s) 51, 277, 693, 727, 1027, 1045
BstAPI GCANNNNNTGC 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 29, 59, 310
BstDEI CTNAG 3 cut(s) 427, 617, 1052
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 79
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 2 cut(s) 81, 353
BstMAI GTCTC 1 cut(s) 921
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 9 cut(s) 67, 73, 76, 97, 305, 338, 359, 777, 842
BstNI CCWGG 1 cut(s) 687
BstSCI CCNGG 3 cut(s) 17, 685, 941
BstSFI CTRYAG 1 cut(s) 25
BstSLI GKGCMC 2 cut(s) 314, 676
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 5 cut(s) 11, 84, 206, 834, 892
BstZI CGGCCG 1 cut(s) 74
BsuRI GGCC 8 cut(s) 76, 160, 312, 346, 362, 474, 591, 674
BtgZI GCGATG 1 cut(s) 817
BtrI CACGTC 1 cut(s) 303
BtsCI GGATG 1 cut(s) 379
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 4 cut(s) 585, 744, 840, 1023
Cac8I GCNNGC 3 cut(s) 29, 59, 310
CfoI GCGC 2 cut(s) 81, 353
Cfr13I GGNCC 7 cut(s) 118, 310, 311, 344, 672, 673, 939
Csp6I GTAC 3 cut(s) 278, 717, 949
CviAII CATG 3 cut(s) 401, 709, 755
CviQI GTAC 3 cut(s) 278, 717, 949
DdeI CTNAG 3 cut(s) 427, 617, 1052
EaeI YGGCCR 4 cut(s) 74, 158, 360, 472
EagI CGGCCG 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 742
EclXI CGGCCG 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 3 cut(s) 314, 676, 744
Eco47I GGWCC 2 cut(s) 118, 939
Eco52I CGGCCG 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 4 cut(s) 683, 768, 894, 1019
EcoICRI GAGCTC 1 cut(s) 742
EcoO109I RGGNCCY 2 cut(s) 311, 672
EcoRII CCWGG 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 3 cut(s) 314, 676, 744
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 3 cut(s) 404, 712, 758
FaqI GGGAC 5 cut(s) 211, 464, 560, 679, 952
FatI CATG 3 cut(s) 400, 708, 754
FauI CCCGC 4 cut(s) 200, 301, 635, 690
FokI GGATG 1 cut(s) 366
FriOI GRGCYC 3 cut(s) 314, 676, 744
FspBI CTAG 2 cut(s) 804, 1060
GlaI GCGC 2 cut(s) 80, 352
GsaI CCCAGC 2 cut(s) 464, 679
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 8 cut(s) 76, 160, 312, 346, 362, 474, 591, 674
HapII CCGG 3 cut(s) 18, 204, 942
HhaI GCGC 2 cut(s) 81, 353
Hin1II CATG 3 cut(s) 404, 712, 758
Hin6I GCGC 2 cut(s) 79, 351
HinP1I GCGC 2 cut(s) 79, 351
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 2 cut(s) 371, 914
HpaII CCGG 3 cut(s) 18, 204, 942
HphI GGTGA 3 cut(s) 16, 158, 911
Hpy166II GTNNAC 3 cut(s) 719, 895, 1041
Hpy188I TCNGA 5 cut(s) 596, 620, 702, 811, 913
Hpy8I GTNNAC 3 cut(s) 719, 895, 1041
HpyAV CCTTC 5 cut(s) 157, 294, 589, 707, 743
HpyCH4III ACNGT 6 cut(s) 51, 277, 693, 727, 1027, 1045
HpyCH4IV ACGT 3 cut(s) 8, 302, 798
HpyCH4V TGCA 7 cut(s) 27, 91, 222, 400, 845, 926, 959
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 73, 76, 97, 305, 338, 359, 777, 842
HpyF3I CTNAG 3 cut(s) 427, 617, 1052
HpySE526I ACGT 3 cut(s) 8, 302, 798
Hsp92II CATG 3 cut(s) 404, 712, 758
HspAI GCGC 2 cut(s) 79, 351
LmnI GCTCC 4 cut(s) 21, 211, 684, 819
Lsp1109I GCAGC 5 cut(s) 11, 84, 206, 834, 892
MaeI CTAG 2 cut(s) 804, 1060
MaeII ACGT 3 cut(s) 8, 302, 798
MaeIII GTNAC 4 cut(s) 4, 439, 451, 1055
MboII GAAGA 7 cut(s) 148, 182, 198, 574, 824, 1022, 1025
MhlI GDGCHC 4 cut(s) 114, 314, 676, 744
MlsI TGGCCA 2 cut(s) 362, 474
MluCI AATT 2 cut(s) 408, 1017
MluNI TGGCCA 2 cut(s) 362, 474
MlyI GAGTC 2 cut(s) 380, 923
Mox20I TGGCCA 2 cut(s) 362, 474
MscI TGGCCA 2 cut(s) 362, 474
MseI TTAA 3 cut(s) 53, 978, 987
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 2 cut(s) 362, 474
MspA1I CMGCKG 2 cut(s) 308, 464
MspI CCGG 3 cut(s) 18, 204, 942
MspR9I CCNGG 3 cut(s) 19, 687, 943
Mva1269I GAATGC 2 cut(s) 528, 961
MvaI CCWGG 1 cut(s) 687
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 9 cut(s) 67, 73, 76, 97, 305, 338, 359, 777, 842
NciI CCSGG 2 cut(s) 19, 943
NlaIII CATG 3 cut(s) 404, 712, 758
NlaIV GGNNCC 9 cut(s) 213, 298, 312, 331, 418, 656, 673, 674, 940
NmeAIII GCCGAG 2 cut(s) 240, 372
NmuCI GTSAC 2 cut(s) 4, 439
PctI GAATGC 2 cut(s) 528, 961
PleI GAGTC 2 cut(s) 379, 922
PpsI GAGTC 2 cut(s) 379, 922
PshBI ATTAAT 1 cut(s) 987
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 1 cut(s) 685
PspFI CCCAGC 2 cut(s) 460, 675
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 9 cut(s) 213, 298, 312, 331, 418, 656, 673, 674, 940
PspOMI GGGCCC 2 cut(s) 310, 672
PspPI GGNCC 7 cut(s) 118, 310, 311, 344, 672, 673, 939
PstI CTGCAG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 3 cut(s) 279, 718, 950
RsaNI GTAC 3 cut(s) 278, 717, 949
RseI CAYNNNNRTG 1 cut(s) 845
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 3 cut(s) 53, 978, 987
Sau96I GGNCC 7 cut(s) 118, 310, 311, 344, 672, 673, 939
ScaI AGTACT 1 cut(s) 950
SchI GAGTC 2 cut(s) 380, 923
ScrFI CCNGG 3 cut(s) 19, 687, 943
SduI GDGCHC 4 cut(s) 114, 314, 676, 744
SfcI CTRYAG 1 cut(s) 25
SinI GGWCC 2 cut(s) 118, 939
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 2 cut(s) 408, 1017
SspMI CTAG 2 cut(s) 804, 1060
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 17, 685, 941
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 6 cut(s) 51, 277, 693, 727, 1027, 1045
TaiI ACGT 3 cut(s) 11, 305, 801
TasI AATT 2 cut(s) 408, 1017
TatI WGTACW 1 cut(s) 948
TauI GCSGC 5 cut(s) 64, 67, 70, 79, 84
Tru1I TTAA 3 cut(s) 53, 978, 987
Tru9I TTAA 3 cut(s) 53, 978, 987
TscAI CASTG 4 cut(s) 592, 751, 847, 1030
TseFI GTSAC 2 cut(s) 4, 439
TseI GCWGC 5 cut(s) 24, 97, 219, 822, 905
Tsp45I GTSAC 2 cut(s) 4, 439
TspDTI ATGAA 1 cut(s) 378
TspRI CASTG 4 cut(s) 592, 751, 847, 1030
VpaK11BI GGWCC 2 cut(s) 118, 939
VspI ATTAAT 1 cut(s) 987
XspI CTAG 2 cut(s) 804, 1060
ZrmI AGTACT 1 cut(s) 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.