RLG00000018854

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003115
Physical Location & Seq
Forward (+)
37934548 .. 37935474
927 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018854

Sequence Viewer

Length: 927 bp
ATGGCAACCCAAAACTCAACTCCGCTGCCCCTCAAGGCCATAGCATACCTGTTCCTTCTAGCTCTTGCAATAACACACACCACCTCAGCCAGAGTCCTTGATGAGGAAACCGATACGCCTCAAACCCAAATACCTGTTTCCACCGTACCTCCTTCTGTGGTCGGTGCCACGTCAGCGGGCCCTCCTCCTGTTGTCAGTGCCACGTCAGCAGGCCCGCGCGCCACAGTGGCCAATGGAGAGTCCTACCATCCGCTTTCATTCTTTCTGCATGATATAATTGGTGGCACCAATCCCTCAGCGAGAGCTGTGACTGGGATTGTGACCAACCCAGCTGTCAATGGCCAAGTCCCCTTTGCCAAACCCAATGGGGCAGTTGTCCAAGGTGTGAATAATGGCATTCCCCAAAACAACAACAACAATGGTATCATCAACAATAACAATGTCCCCTTCTTCACTGGCCTCGGAGGAAACATCAACAACAACCTCAGACAAAACAACAACTTTATTGGCGGGAACAATGGGTTCCCCGTTGTGAATGGGGCCCAGCTCCCGCCAGGACAGTCCCTTCAGAAGCTCATGTTCGGCACACTCACGGTCTTTGACGATGAGCTCACCGAAGGACATGAGCTTGGCTCTGGCTTGTTGGGCAAGGCACAAGGATTTTACATCGCTAGTTCCGAAGATGGAAGCAGCCAGACCATTGCTTTCACTGCAATGTTTGAGAGTGGTGGGTATGCTGATAGCCTCACATTCTTTGGGGTTCACAGAGTAGCTGCTTCAGAGTCTCACCTTGCAGTTATGGGAGGGACCGGGAAGTACTTGAATGCAAAAGGGTATGCTTTGGTTAAGACCATTAATGGAGTGAATGAGCAAAATACTGATGGAATTGACACTGTTCTTCAGTTTACTGTGTATCTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

309

Amino Acids

32.01

Weight (kDa)

5.24

Isoelectric Point (pI)

26.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 175 - 306 1.3e-28 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 164, 284
AccII CGCG 2 cut(s) 217, 219
AciI CCGC 6 cut(s) 23, 176, 215, 251, 510, 551
AcoI YGGCCR 2 cut(s) 228, 340
AcuI CTGAAG 3 cut(s) 551, 762, 884
AfaI GTAC 2 cut(s) 147, 818
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 1 cut(s) 823
AjiI CACGTC 2 cut(s) 171, 204
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 8 cut(s) 62, 305, 332, 547, 574, 610, 628, 773
AluI AGCT 8 cut(s) 62, 305, 332, 547, 574, 610, 628, 773
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 1 cut(s) 789
AoxI GGCC 7 cut(s) 36, 178, 211, 228, 340, 457, 540
ApaI GGGCCC 2 cut(s) 182, 544
ApeKI GCWGC 3 cut(s) 25, 690, 773
AseI ATTAAT 1 cut(s) 855
AspLEI GCGC 2 cut(s) 219, 221
AspS9I GGNCC 6 cut(s) 178, 179, 212, 540, 541, 807
AsuC2I CCSGG 1 cut(s) 811
AsuHPI GGTGA 2 cut(s) 604, 779
AvaII GGWCC 1 cut(s) 807
BaeGI GKGCMC 2 cut(s) 182, 544
BaeI ACNNNNGTAYC 2 cut(s) 406, 439
BalI TGGCCA 2 cut(s) 230, 342
BanI GGYRCC 2 cut(s) 164, 284
BanII GRGCYC 3 cut(s) 182, 544, 612
Bbv12I GWGCWC 1 cut(s) 612
BbvCI CCTCAGC 2 cut(s) 85, 295
BbvI GCAGC 3 cut(s) 12, 702, 760
BccI CCATC 3 cut(s) 255, 677, 875
BciT130I CCWGG 1 cut(s) 555
BcnI CCSGG 1 cut(s) 811
BcoDI GTCTC 1 cut(s) 789
BfaI CTAG 3 cut(s) 59, 672, 925
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 3 cut(s) 26, 691, 774
BlsI GCNGC 3 cut(s) 27, 692, 775
BmcAI AGTACT 1 cut(s) 818
Bme1390I CCNGG 2 cut(s) 555, 811
Bme18I GGWCC 1 cut(s) 807
BmgBI CACGTC 2 cut(s) 171, 204
BmgT120I GGNCC 6 cut(s) 178, 179, 212, 540, 541, 807
BmiI GGNNCC 7 cut(s) 166, 180, 286, 524, 541, 542, 808
BmrFI CCNGG 2 cut(s) 555, 811
BmrI ACTGGG 1 cut(s) 321
BmuI ACTGGG 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 617, 649
Bpu10I CCTNAGC 2 cut(s) 85, 295
BpuEI CTTGAG 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 811
BsaJI CCNNGG 2 cut(s) 379, 460
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 2 cut(s) 316, 460
Bse3DI GCAATG 2 cut(s) 699, 720
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 379, 460
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMI GCAATG 2 cut(s) 699, 720
BseMII CTCAG 3 cut(s) 99, 309, 499
BseNI ACTGG 2 cut(s) 316, 460
BsePI GCGCGC 1 cut(s) 217
BseRI GAGGAG 1 cut(s) 174
BseSI GKGCMC 2 cut(s) 182, 544
BseXI GCAGC 3 cut(s) 12, 702, 760
BseYI CCCAGC 2 cut(s) 328, 543
Bsh1236I CGCG 2 cut(s) 217, 219
BshFI GGCC 7 cut(s) 38, 180, 213, 230, 342, 459, 542
BshNI GGYRCC 2 cut(s) 164, 284
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 1 cut(s) 810
BslFI GGGAC 4 cut(s) 332, 428, 547, 820
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 789
BsmFI GGGAC 4 cut(s) 332, 428, 547, 820
BsmI GAATGC 2 cut(s) 396, 829
BsnI GGCC 7 cut(s) 38, 180, 213, 230, 342, 459, 542
Bsp120I GGGCCC 2 cut(s) 178, 540
Bsp1286I GDGCHC 3 cut(s) 182, 544, 612
BspACI CCGC 6 cut(s) 23, 176, 215, 251, 510, 551
BspANI GGCC 7 cut(s) 38, 180, 213, 230, 342, 459, 542
BspCNI CTCAG 3 cut(s) 98, 308, 498
BspFNI CGCG 2 cut(s) 217, 219
BspLI GGNNCC 7 cut(s) 166, 180, 286, 524, 541, 542, 808
BspT107I GGYRCC 2 cut(s) 164, 284
BsrDI GCAATG 2 cut(s) 699, 720
BsrI ACTGG 2 cut(s) 316, 460
BssECI CCNNGG 2 cut(s) 379, 460
BssHII GCGCGC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 6 cut(s) 145, 226, 561, 595, 895, 910
BstC8I GCNNGC 4 cut(s) 178, 211, 215, 219
BstDEI CTNAG 4 cut(s) 85, 295, 485, 917
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 2 cut(s) 217, 219
BstHHI GCGC 2 cut(s) 219, 221
BstMAI GTCTC 1 cut(s) 789
BstMWI GCNNNNNNNGC 5 cut(s) 173, 206, 227, 645, 710
BstNI CCWGG 1 cut(s) 555
BstSCI CCNGG 2 cut(s) 553, 809
BstSLI GKGCMC 2 cut(s) 182, 544
BstUI CGCG 2 cut(s) 217, 219
BstV1I GCAGC 3 cut(s) 12, 702, 760
BsuRI GGCC 7 cut(s) 38, 180, 213, 230, 342, 459, 542
BtgZI GCGATG 1 cut(s) 652
BtrI CACGTC 2 cut(s) 171, 204
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 5 cut(s) 202, 231, 453, 708, 891
Cac8I GCNNGC 4 cut(s) 178, 211, 215, 219
CfoI GCGC 2 cut(s) 219, 221
Cfr13I GGNCC 6 cut(s) 178, 179, 212, 540, 541, 807
Csp6I GTAC 2 cut(s) 146, 817
CviAII CATG 3 cut(s) 269, 577, 623
CviQI GTAC 2 cut(s) 146, 817
DdeI CTNAG 4 cut(s) 85, 295, 485, 917
EaeI YGGCCR 2 cut(s) 228, 340
Ecl136II GAGCTC 1 cut(s) 610
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 3 cut(s) 182, 544, 612
Eco47I GGWCC 1 cut(s) 807
Eco53kI GAGCTC 1 cut(s) 610
Eco57I CTGAAG 3 cut(s) 551, 762, 884
EcoICRI GAGCTC 1 cut(s) 610
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 2 cut(s) 179, 540
EcoRII CCWGG 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 379
EcoT38I GRGCYC 3 cut(s) 182, 544, 612
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 3 cut(s) 272, 580, 626
FaiI YATR 9 cut(s) 41, 46, 270, 275, 578, 624, 735, 800, 837
FaqI GGGAC 4 cut(s) 332, 428, 547, 820
FatI CATG 3 cut(s) 268, 576, 622
FauI CCCGC 4 cut(s) 169, 222, 503, 558
Fnu4HI GCNGC 3 cut(s) 26, 691, 774
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 3 cut(s) 182, 544, 612
Fsp4HI GCNGC 3 cut(s) 26, 691, 774
FspBI CTAG 3 cut(s) 59, 672, 925
GlaI GCGC 2 cut(s) 218, 220
GluI GCNGC 3 cut(s) 26, 691, 774
GsaI CCCAGC 2 cut(s) 332, 547
HaeIII GGCC 7 cut(s) 38, 180, 213, 230, 342, 459, 542
HapII CCGG 1 cut(s) 810
HhaI GCGC 2 cut(s) 219, 221
Hin1II CATG 3 cut(s) 272, 580, 626
Hin6I GCGC 2 cut(s) 217, 219
HinP1I GCGC 2 cut(s) 217, 219
HinfI GANTC 3 cut(s) 93, 239, 782
HpaII CCGG 1 cut(s) 810
HphI GGTGA 2 cut(s) 604, 779
Hpy166II GTNNAC 2 cut(s) 763, 906
Hpy188I TCNGA 5 cut(s) 464, 488, 570, 679, 781
Hpy8I GTNNAC 2 cut(s) 763, 906
HpyAV CCTTC 5 cut(s) 65, 162, 457, 575, 611
HpyCH4III ACNGT 6 cut(s) 145, 226, 561, 595, 895, 910
HpyCH4IV ACGT 2 cut(s) 170, 203
HpyCH4V TGCA 5 cut(s) 68, 268, 713, 794, 827
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 206, 227, 645, 710
HpyF3I CTNAG 4 cut(s) 85, 295, 485, 917
HpySE526I ACGT 2 cut(s) 170, 203
Hsp92II CATG 3 cut(s) 272, 580, 626
HspAI GCGC 2 cut(s) 217, 219
LmnI GCTCC 1 cut(s) 552
Lsp1109I GCAGC 3 cut(s) 12, 702, 760
MaeI CTAG 3 cut(s) 59, 672, 925
MaeII ACGT 2 cut(s) 170, 203
MaeIII GTNAC 3 cut(s) 307, 319, 920
MboII GAAGA 3 cut(s) 442, 692, 890
MhlI GDGCHC 3 cut(s) 182, 544, 612
MlsI TGGCCA 2 cut(s) 230, 342
MluCI AATT 2 cut(s) 276, 885
MluNI TGGCCA 2 cut(s) 230, 342
MlyI GAGTC 3 cut(s) 102, 248, 791
Mox20I TGGCCA 2 cut(s) 230, 342
MscI TGGCCA 2 cut(s) 230, 342
MseI TTAA 2 cut(s) 846, 855
MslI CAYNNNNRTG 1 cut(s) 713
Msp20I TGGCCA 2 cut(s) 230, 342
MspA1I CMGCKG 3 cut(s) 25, 176, 332
MspI CCGG 1 cut(s) 810
MspR9I CCNGG 2 cut(s) 555, 811
Mva1269I GAATGC 2 cut(s) 396, 829
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 2 cut(s) 217, 219
MwoI GCNNNNNNNGC 5 cut(s) 173, 206, 227, 645, 710
NciI CCSGG 1 cut(s) 811
NlaIII CATG 3 cut(s) 272, 580, 626
NlaIV GGNNCC 7 cut(s) 166, 180, 286, 524, 541, 542, 808
NmuCI GTSAC 2 cut(s) 307, 319
PauI GCGCGC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PctI GAATGC 2 cut(s) 396, 829
PkrI GCNGC 3 cut(s) 27, 692, 775
PleI GAGTC 3 cut(s) 101, 247, 790
PpsI GAGTC 3 cut(s) 101, 247, 790
PshBI ATTAAT 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 553
PspFI CCCAGC 2 cut(s) 328, 543
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 7 cut(s) 166, 180, 286, 524, 541, 542, 808
PspOMI GGGCCC 2 cut(s) 178, 540
PspPI GGNCC 6 cut(s) 178, 179, 212, 540, 541, 807
PteI GCGCGC 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 147, 818
RsaNI GTAC 2 cut(s) 146, 817
RseI CAYNNNNRTG 1 cut(s) 713
SacI GAGCTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 846, 855
SatI GCNGC 3 cut(s) 26, 691, 774
Sau96I GGNCC 6 cut(s) 178, 179, 212, 540, 541, 807
ScaI AGTACT 1 cut(s) 818
SchI GAGTC 3 cut(s) 102, 248, 791
ScrFI CCNGG 2 cut(s) 555, 811
SduI GDGCHC 3 cut(s) 182, 544, 612
SinI GGWCC 1 cut(s) 807
SmiMI CAYNNNNRTG 1 cut(s) 713
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 2 cut(s) 276, 885
SsiI CCGC 6 cut(s) 23, 176, 215, 251, 510, 551
SspMI CTAG 3 cut(s) 59, 672, 925
SstI GAGCTC 1 cut(s) 612
StyD4I CCNGG 2 cut(s) 553, 809
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 6 cut(s) 145, 226, 561, 595, 895, 910
TaiI ACGT 2 cut(s) 173, 206
TasI AATT 2 cut(s) 276, 885
TatI WGTACW 1 cut(s) 816
Tru1I TTAA 2 cut(s) 846, 855
Tru9I TTAA 2 cut(s) 846, 855
TscAI CASTG 5 cut(s) 202, 231, 460, 715, 898
TseFI GTSAC 2 cut(s) 307, 319
TseI GCWGC 3 cut(s) 25, 690, 773
Tsp45I GTSAC 2 cut(s) 307, 319
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 5 cut(s) 202, 231, 460, 715, 898
VpaK11BI GGWCC 1 cut(s) 807
VspI ATTAAT 1 cut(s) 855
XagI CCTNNNNNAGG 1 cut(s) 101
XspI CTAG 3 cut(s) 59, 672, 925
ZrmI AGTACT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.