MD12G1110900.v1.1

phytochrome kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014149
Physical Location & Seq
Forward (+)
17566093 .. 17568140
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1110900.v1.1.491

Sequence Viewer

Length: 1827 bp
ATGCTCAGAGACAAAGATACAGATCACGCTGCAACTGCAACCGCAAATGTTGATCAACAACCGCGATACTACTACAACGACAACACCATTAAGCAGCAGCAGCTTCATGTAGACGTGGATCGATATTCGAGTACTACTAGTATCAGTGTTACTGCTGATCAAGAAATCAGCATTTTTGACGCCCACAAATACTTCAACCAACTAAGCATCAACAACCACATTGATGGCCAAAGCCATAAGGTGCAGGTTTCTGATGGGCTGTCTGGCGTTTCGAGATTCTCTTGGGCTGCTGGCTCTCCCTCAGTATCTTCAGTTGATGATGCTGCTATTAACTACCCTCTGCGTCTCCGAGCTCGTTCTTTCCATGTCGTTTCTGCCAGTGCCACCCCAACTGCTTCCTCCGAAGCCAGTTGGAACAGCCAGACTGGCCTCCTATCCAATCCTCGCTGCACTACCACCGCCACCAATAGTAATGTCGCATTCATGGTGCCTTTGCGCAGCCGGACTAGTACAAATCATCCTCCTCGTCCTCATCACGATGACCATCAGATTACTAATAAGAAGAAATTAAGAGGATGGTCCAAGACAATGTCATTGAGTCCAAGATGGCTTACTGCTTTTCCGAGGAGGTCAGCGAGATGCCCCTGCTCGGGCAAGAAATCAGTACAAGTTGTTGCTCAGGAAAAGAAATCAAGCATGTCATCATCATCATCAATAGTCAGAGTTGGAGGCGGAGATATTTATAACATGCCGGCCCAATTAATTCTCACCAAGGATGAGATACCACCAGCTGCTCCAACTACTAGTGTTATTACAGCTGCCAATTTGAACAACTCTTCGATTGCAAGGCCTCGTGACGACAATCGCCATGTGCTCTGGGGTGATGAACGTCGTCGGAATTGGGAACGCCAAGTCAGCAATACAACCATCAATGTGCAACCAGCCGCAAACTCTACCACTACTACGACTAGCTGTATTAGTACTGCTGCTTCTGGTGGCGGATTTAGCTTTCCGATACTAAAATTCAATGGGGACGGGGGAACTTCCTTGCCATCGGCAACAACTTCAATCCGGAACCCATCAAACAGTACTACTGATCATGTTCAATGCGGCCGACTAATTGGTGATGTTAATAATAAAGATGAGGATGTGGGAAGCGATGCAAGCTCTGACTTGTTCGAAATCGAAAGCTTCTCAACCAGTACTACTCAAACGCTGCAAGCAACTAATGCCGATATGCCCACCTACCCTCCACCATCGTCATCCATGTTTGAAAATTACTACCGCACTCGCCGGGATTCTTTTTCATTGGTTGAGGATGCAATTAGCTTCACTGCTGCTCGACGTTTGAGTGCTAATAACAATTCCCATCGAACAAGCTTAGATCGTCGTCAACATGACCCGCCTATGGCATCGTTATCGACCGATCAATACTGTTACGAACCAAGTGAGGCCAGCATCGACTGGAGCGTCACTACGGCAGAGGGCTTTGATCGATCCGACCAATACGATCACGACCACCATGATCACCATGATGATGCTGACTTGGGAACTAAGGTAATGACGAGCAGCAAACGACGGCCGCCTTCACTGGGAAGTAATAACAATAATATGGGGTTGTTGAGCTGTCGGCGGGACAAGGCCGTCAGTGTGGGGCCCAACCCAGTGAGACTAATGGCCATGCCTGCTGCTCCTGCTGATCATGACTACTGTCAGAGTCGACATAGGGGTGTAGGAGTGGAACCTACAAGTCTATCAGTGTGTTCAAACAACTCCAGAGGTACGATGCATGTGGGAAGTAGGTCCGCAGCCTTAGCTAGACAATAA

Protein Analysis

609

Amino Acids

66.46

Weight (kDa)

8.31

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04190
fragaria_vesca FvH4_4g10470
malus_domestica MD12G1110900.v1.1
prunus_persica Prupe.7G023100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0495011
rosa_laevigata RLG00000022656
rosa_multiflora Rmu_co8188214.1_g000001 Rmu_ssc0000058.1_g000001
rosa_roxburghii Rroxscaffold_4G00319360 Rroxscaffold_6G00390850
rosa_rugosa Rorug03G0269300
rosa_samantha Rh3BG353200 Rh3CG313700 Rh3CG350100 Rh3CG350300 Rh3DG352600
rosa_wichuraiana Rw3G027740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AasI GACNNNNNNGTC 2 cut(s) 1469, 1643
Acc16I TGCGCA 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 487
AccI GTMKAC 2 cut(s) 111, 1720
AccII CGCG 1 cut(s) 64
AccIII TCCGGA 1 cut(s) 1071
AclWI GGATC 2 cut(s) 126, 1491
AcoI YGGCCR 4 cut(s) 226, 1111, 1580, 1677
AcsI RAATTY 1 cut(s) 1022
AcuI CTGAAG 1 cut(s) 294
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 7 cut(s) 133, 511, 666, 982, 1090, 1204, 1783
AfiI CCNNNNNNNGG 4 cut(s) 649, 650, 1408, 1592
AgsI TTSAA 7 cut(s) 196, 829, 1027, 1068, 1106, 1274, 1767
AhlI ACTAGT 3 cut(s) 137, 506, 803
AjiI CACGTC 1 cut(s) 115
Alw21I GWGCWC 2 cut(s) 355, 876
Alw26I GTCTC 3 cut(s) 3, 350, 1663
AlwI GGATC 2 cut(s) 126, 1491
Ama87I CYCGRG 1 cut(s) 649
Aor13HI TCCGGA 1 cut(s) 1071
ApaI GGGCCC 1 cut(s) 1659
ApoI RAATTY 1 cut(s) 1022
AseI ATTAAT 1 cut(s) 761
AspLEI GCGC 1 cut(s) 498
AspS9I GGNCC 5 cut(s) 579, 754, 1655, 1656, 1803
AsuC2I CCSGG 1 cut(s) 1295
AsuHPI GGTGA 4 cut(s) 760, 893, 1136, 1520
AsuII TTCGAA 1 cut(s) 1179
AvaI CYCGRG 1 cut(s) 649
AvaII GGWCC 2 cut(s) 579, 1803
BaeGI GKGCMC 1 cut(s) 1659
BalI TGGCCA 2 cut(s) 228, 1679
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 2 cut(s) 355, 1659
BarI GAAGNNNNNNTAC 2 cut(s) 973, 1005
BauI CACGAG 1 cut(s) 852
Bbv12I GWGCWC 2 cut(s) 355, 876
BceAI ACGGC 3 cut(s) 1494, 1595, 1628
BcgI CGANNNNNNTGC 2 cut(s) 1401, 1435
BclI TGATCA 5 cut(s) 52, 157, 1096, 1525, 1699
BcnI CCSGG 1 cut(s) 1295
BcoDI GTCTC 3 cut(s) 3, 350, 1663
BcuI ACTAGT 3 cut(s) 137, 506, 803
BfaI CTAG 5 cut(s) 138, 507, 804, 969, 1818
BfuAI ACCTGC 1 cut(s) 235
BglI GCCNNNNNGGC 1 cut(s) 426
BmcAI AGTACT 4 cut(s) 133, 982, 1090, 1204
Bme1390I CCNGG 1 cut(s) 1295
Bme18I GGWCC 2 cut(s) 579, 1803
BmeT110I CYCGRG 1 cut(s) 649
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 5 cut(s) 579, 754, 1655, 1656, 1803
BmiI GGNNCC 5 cut(s) 489, 1076, 1656, 1657, 1743
BmrFI CCNGG 1 cut(s) 1295
BmrI ACTGGG 2 cut(s) 1601, 1658
BmsI GCATC 9 cut(s) 216, 310, 629, 1150, 1309, 1421, 1467, 1528, 1776
BmuI ACTGGG 2 cut(s) 1601, 1658
BoxI GACNNNNGTC 1 cut(s) 1710
BpmI CTGGAG 2 cut(s) 1486, 1759
Bpu10I CCTNAGC 2 cut(s) 678, 1813
Bpu14I TTCGAA 1 cut(s) 1179
BpuMI CCSGG 1 cut(s) 1295
Bsa29I ATCGAT 2 cut(s) 121, 1495
BsaBI GATNNNNATC 2 cut(s) 21, 543
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 623, 771
BsaWI WCCGGW 1 cut(s) 1071
BsaXI ACNNNNNCTCC 2 cut(s) 1234, 1264
Bsc4I CCNNNNNNNGG 4 cut(s) 649, 650, 1408, 1592
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 7 cut(s) 378, 408, 430, 1200, 1469, 1596, 1664
Bse8I GATNNNNATC 2 cut(s) 21, 543
BseAI TCCGGA 1 cut(s) 1071
BseCI ATCGAT 2 cut(s) 121, 1495
BseDI CCNNGG 2 cut(s) 623, 771
BseGI GGATG 6 cut(s) 517, 581, 781, 1153, 1262, 1324
BseJI GATNNNNATC 2 cut(s) 21, 543
BseLI CCNNNNNNNGG 4 cut(s) 649, 650, 1408, 1592
BseMII CTCAG 3 cut(s) 19, 315, 692
BseNI ACTGG 7 cut(s) 378, 408, 430, 1200, 1469, 1596, 1664
BseRI GAGGAG 2 cut(s) 513, 640
BseSI GKGCMC 1 cut(s) 1659
BseX3I CGGCCG 2 cut(s) 1111, 1580
BsgI GTGCAG 2 cut(s) 263, 433
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 3 cut(s) 1114, 1425, 1583
BshNI GGYRCC 1 cut(s) 487
BshVI ATCGAT 2 cut(s) 121, 1495
BsiEI CGRYCG 3 cut(s) 1114, 1425, 1583
BsiHKAI GWGCWC 2 cut(s) 355, 876
BsiHKCI CYCGRG 1 cut(s) 649
BsiSI CCGG 4 cut(s) 502, 752, 1072, 1294
BslFI GGGAC 2 cut(s) 1046, 1649
BslI CCNNNNNNNGG 4 cut(s) 649, 650, 1408, 1592
BsmAI GTCTC 3 cut(s) 3, 350, 1663
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 2 cut(s) 1046, 1649
BsmI GAATGC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 649
Bsp119I TTCGAA 1 cut(s) 1179
Bsp120I GGGCCC 1 cut(s) 1655
Bsp1286I GDGCHC 3 cut(s) 355, 876, 1659
Bsp13I TCCGGA 1 cut(s) 1071
BspCNI CTCAG 3 cut(s) 18, 314, 691
BspDI ATCGAT 2 cut(s) 121, 1495
BspEI TCCGGA 1 cut(s) 1071
BspFNI CGCG 1 cut(s) 64
BspHI TCATGA 1 cut(s) 1702
BspLI GGNNCC 5 cut(s) 489, 1076, 1656, 1657, 1743
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 2 cut(s) 126, 1491
BspT104I TTCGAA 1 cut(s) 1179
BspT107I GGYRCC 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 7 cut(s) 378, 408, 430, 1200, 1469, 1596, 1664
BssAI RCCGGY 1 cut(s) 751
BssECI CCNNGG 2 cut(s) 623, 771
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 1 cut(s) 771
Bst2BI CACGAG 1 cut(s) 852
Bst4CI ACNGT 3 cut(s) 1088, 1436, 1712
Bst6I CTCTTC 1 cut(s) 841
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 1229
BstBI TTCGAA 1 cut(s) 1179
BstC8I GCNNGC 6 cut(s) 292, 753, 1165, 1221, 1456, 1686
BstDEI CTNAG 7 cut(s) 5, 203, 301, 678, 1381, 1554, 1813
BstF5I GGATG 6 cut(s) 517, 581, 781, 1153, 1262, 1324
BstFNI CGCG 1 cut(s) 64
BstHHI GCGC 1 cut(s) 498
BstMAI GTCTC 3 cut(s) 3, 350, 1663
BstMCI CGRYCG 3 cut(s) 1114, 1425, 1583
BstNSI RCATGY 3 cut(s) 700, 751, 1793
BstPAI GACNNNNGTC 1 cut(s) 1710
BstSCI CCNGG 1 cut(s) 1293
BstSLI GKGCMC 1 cut(s) 1659
BstUI CGCG 1 cut(s) 64
BstXI CCANNNNNNTGG 1 cut(s) 224
BstZI CGGCCG 2 cut(s) 1111, 1580
Bsu15I ATCGAT 2 cut(s) 121, 1495
BsuTUI ATCGAT 2 cut(s) 121, 1495
BtgZI GCGATG 1 cut(s) 1173
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 6 cut(s) 517, 581, 781, 1153, 1262, 1324
BtsI GCAGTG 1 cut(s) 1332
BtsIMutI CAGTG 7 cut(s) 151, 385, 1332, 1589, 1654, 1671, 1764
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 6 cut(s) 292, 753, 1165, 1221, 1456, 1686
CciI TCATGA 1 cut(s) 1702
CfoI GCGC 1 cut(s) 498
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 5 cut(s) 579, 754, 1655, 1656, 1803
ClaI ATCGAT 2 cut(s) 121, 1495
CseI GACGC 3 cut(s) 188, 332, 1459
Csp6I GTAC 7 cut(s) 132, 510, 665, 981, 1089, 1203, 1782
CviQI GTAC 7 cut(s) 132, 510, 665, 981, 1089, 1203, 1782
DdeI CTNAG 7 cut(s) 5, 203, 301, 678, 1381, 1554, 1813
DrdI GACNNNNNNGTC 2 cut(s) 1469, 1643
DseDI GACNNNNNNGTC 2 cut(s) 1469, 1643
EaeI YGGCCR 4 cut(s) 226, 1111, 1580, 1677
EagI CGGCCG 2 cut(s) 1111, 1580
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
EciI GGCGGA 2 cut(s) 747, 1014
Ecl136II GAGCTC 1 cut(s) 353
EclXI CGGCCG 2 cut(s) 1111, 1580
Eco130I CCWWGG 1 cut(s) 771
Eco147I AGGCCT 1 cut(s) 850
Eco24I GRGCYC 2 cut(s) 355, 1659
Eco47I GGWCC 2 cut(s) 579, 1803
Eco52I CGGCCG 2 cut(s) 1111, 1580
Eco53kI GAGCTC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 649
EcoICRI GAGCTC 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 1655
EcoT14I CCWWGG 1 cut(s) 771
EcoT22I ATGCAT 1 cut(s) 1791
EcoT38I GRGCYC 2 cut(s) 355, 1659
ErhI CCWWGG 1 cut(s) 771
Esp3I CGTCTC 1 cut(s) 350
FaqI GGGAC 2 cut(s) 1046, 1649
FauI CCCGC 2 cut(s) 1410, 1626
FbaI TGATCA 5 cut(s) 52, 157, 1096, 1525, 1699
FblI GTMKAC 2 cut(s) 111, 1720
FokI GGATG 6 cut(s) 504, 588, 788, 1160, 1249, 1331
FriOI GRGCYC 2 cut(s) 355, 1659
FspBI CTAG 5 cut(s) 138, 507, 804, 969, 1818
FspI TGCGCA 1 cut(s) 497
GlaI GCGC 1 cut(s) 497
GsuI CTGGAG 2 cut(s) 1486, 1759
HapII CCGG 4 cut(s) 502, 752, 1072, 1294
HgaI GACGC 3 cut(s) 188, 332, 1459
HhaI GCGC 1 cut(s) 498
Hin1I GRCGYC 1 cut(s) 180
Hin6I GCGC 1 cut(s) 496
HinP1I GCGC 1 cut(s) 496
HincII GTYRAC 2 cut(s) 1394, 1721
HindII GTYRAC 2 cut(s) 1394, 1721
HindIII AAGCTT 2 cut(s) 1189, 1378
HinfI GANTC 4 cut(s) 276, 598, 1298, 1717
HpaII CCGG 4 cut(s) 502, 752, 1072, 1294
HphI GGTGA 4 cut(s) 760, 893, 1136, 1520
Hpy166II GTNNAC 3 cut(s) 112, 1394, 1721
Hpy188III TCNNGA 9 cut(s) 161, 273, 536, 680, 854, 1072, 1514, 1703, 1776
Hpy8I GTNNAC 3 cut(s) 112, 1394, 1721
Hpy99I CGWCG 5 cut(s) 894, 897, 1347, 1392, 1581
HpyAV CCTTC 1 cut(s) 1596
HpyCH4III ACNGT 3 cut(s) 1088, 1436, 1712
HpyCH4IV ACGT 3 cut(s) 114, 889, 1345
HpyF3I CTNAG 7 cut(s) 5, 203, 301, 678, 1381, 1554, 1813
HpySE526I ACGT 3 cut(s) 114, 889, 1345
Hsp92I GRCGYC 1 cut(s) 180
HspAI GCGC 1 cut(s) 496
Kpn2I TCCGGA 1 cut(s) 1071
KroI GCCGGC 1 cut(s) 751
KroNI GCCGGC 1 cut(s) 753
Ksp22I TGATCA 5 cut(s) 52, 157, 1096, 1525, 1699
LmnI GCTCC 3 cut(s) 799, 1467, 1696
LweI GCATC 9 cut(s) 216, 310, 629, 1150, 1309, 1421, 1467, 1528, 1776
MaeI CTAG 5 cut(s) 138, 507, 804, 969, 1818
MaeII ACGT 3 cut(s) 114, 889, 1345
MaeIII GTNAC 4 cut(s) 148, 854, 1436, 1471
MboII GAAGA 3 cut(s) 300, 574, 828
MhlI GDGCHC 3 cut(s) 355, 876, 1659
MlsI TGGCCA 2 cut(s) 228, 1679
MluNI TGGCCA 2 cut(s) 228, 1679
MlyI GAGTC 2 cut(s) 607, 1726
MmeI TCCRAC 5 cut(s) 392, 706, 821, 875, 1524
Mox20I TGGCCA 2 cut(s) 228, 1679
Mph1103I ATGCAT 1 cut(s) 1791
MroI TCCGGA 1 cut(s) 1071
MroNI GCCGGC 1 cut(s) 751
MscI TGGCCA 2 cut(s) 228, 1679
MseI TTAA 5 cut(s) 90, 330, 569, 761, 1131
MslI CAYNNNNRTG 6 cut(s) 222, 537, 932, 1533, 1536, 1728
Msp20I TGGCCA 2 cut(s) 228, 1679
MspA1I CMGCKG 2 cut(s) 791, 818
MspI CCGG 4 cut(s) 502, 752, 1072, 1294
MspR9I CCNGG 1 cut(s) 1295
Mva1269I GAATGC 1 cut(s) 479
MvnI CGCG 1 cut(s) 64
NaeI GCCGGC 1 cut(s) 753
NciI CCSGG 1 cut(s) 1295
NgoMIV GCCGGC 1 cut(s) 751
NlaIV GGNNCC 5 cut(s) 489, 1076, 1656, 1657, 1743
NmuCI GTSAC 2 cut(s) 854, 1471
NsbI TGCGCA 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 1791
NspI RCATGY 3 cut(s) 700, 751, 1793
NspV TTCGAA 1 cut(s) 1179
PagI TCATGA 1 cut(s) 1702
PceI AGGCCT 1 cut(s) 850
PcsI WCGNNNNNNNCGW 1 cut(s) 1467
PctI GAATGC 1 cut(s) 479
PdiI GCCGGC 1 cut(s) 753
PfeI GAWTC 2 cut(s) 276, 1298
PflFI GACNNNGTC 1 cut(s) 589
PleI GAGTC 2 cut(s) 606, 1725
PpsI GAGTC 2 cut(s) 606, 1725
PshAI GACNNNNGTC 1 cut(s) 1710
PshBI ATTAAT 1 cut(s) 761
PsiI TTATAA 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 355
PspN4I GGNNCC 5 cut(s) 489, 1076, 1656, 1657, 1743
PspOMI GGGCCC 1 cut(s) 1655
PspPI GGNCC 5 cut(s) 579, 754, 1655, 1656, 1803
PsyI GACNNNGTC 1 cut(s) 589
PvuII CAGCTG 2 cut(s) 791, 818
RsaI GTAC 7 cut(s) 133, 511, 666, 982, 1090, 1204, 1783
RsaNI GTAC 7 cut(s) 132, 510, 665, 981, 1089, 1203, 1782
RseI CAYNNNNRTG 6 cut(s) 222, 537, 932, 1533, 1536, 1728
SacI GAGCTC 1 cut(s) 355
SalI GTCGAC 1 cut(s) 1719
SaqAI TTAA 5 cut(s) 90, 330, 569, 761, 1131
Sau96I GGNCC 5 cut(s) 579, 754, 1655, 1656, 1803
ScaI AGTACT 4 cut(s) 133, 982, 1090, 1204
SchI GAGTC 2 cut(s) 607, 1726
ScrFI CCNGG 1 cut(s) 1295
SduI GDGCHC 3 cut(s) 355, 876, 1659
SfaNI GCATC 9 cut(s) 216, 310, 629, 1150, 1309, 1421, 1467, 1528, 1776
SfuI TTCGAA 1 cut(s) 1179
SinI GGWCC 2 cut(s) 579, 1803
SmiMI CAYNNNNRTG 6 cut(s) 222, 537, 932, 1533, 1536, 1728
SpeI ACTAGT 3 cut(s) 137, 506, 803
SseBI AGGCCT 1 cut(s) 850
SspMI CTAG 5 cut(s) 138, 507, 804, 969, 1818
SstI GAGCTC 1 cut(s) 355
StuI AGGCCT 1 cut(s) 850
StyD4I CCNGG 1 cut(s) 1293
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 3 cut(s) 1088, 1436, 1712
TaiI ACGT 3 cut(s) 117, 892, 1348
TaqII GACCGA 1 cut(s) 1439
TatI WGTACW 6 cut(s) 131, 509, 664, 980, 1088, 1202
TauI GCSGC 3 cut(s) 947, 1113, 1585
TfiI GAWTC 2 cut(s) 276, 1298
Tru1I TTAA 5 cut(s) 90, 330, 569, 761, 1131
Tru9I TTAA 5 cut(s) 90, 330, 569, 761, 1131
TscAI CASTG 7 cut(s) 151, 385, 1339, 1596, 1654, 1671, 1764
TseFI GTSAC 2 cut(s) 854, 1471
Tsp45I GTSAC 2 cut(s) 854, 1471
TspDTI ATGAA 4 cut(s) 95, 472, 900, 1296
TspRI CASTG 7 cut(s) 151, 385, 1339, 1596, 1654, 1671, 1764
Tth111I GACNNNGTC 1 cut(s) 589
VpaK11BI GGWCC 2 cut(s) 579, 1803
VspI ATTAAT 1 cut(s) 761
XapI RAATTY 1 cut(s) 1022
XceI RCATGY 3 cut(s) 700, 751, 1793
XmiI GTMKAC 2 cut(s) 111, 1720
XspI CTAG 5 cut(s) 138, 507, 804, 969, 1818
ZrmI AGTACT 4 cut(s) 133, 982, 1090, 1204
Zsp2I ATGCAT 1 cut(s) 1791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.