Rw3G027740

phytochrome kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0014149
Physical Location & Seq
Forward (+)
40045780 .. 40047570
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027740.1

Sequence Viewer

Length: 1791 bp
ATGGAGAGATCAAAAGGTGTCAGCAGTGGCGGCAGCAGCAACAGCAACAGCATATCTCAGAAAGAGTACATCCTTGGAGACAGAAATGTATCATTCTCTACTTATCGCAGACCAGATCACGAAAAGGATGGCCTTGCGGCAGGTGATGACTCCACTGAAATAAGCATTTTTGAGGCCCAGAAGTACTTCAACGAAAGCATCATTGATGCCCACAAGGTGAGCAACAACAACAATAATGGTGCTATTAATAACATTAATAACAGAGCAATATCTTCTCGTGGAACAGCCAACAATGTCAGCATCGACAACATGGAACAGCCTTCGAGATTCTCGTGGGCGGCTTCCTCATCCGTTCATGATGAATACTCCAGTATTAACATGGCAAGGAATAACTACAGAGCTCGGTCTTTTCATGCGGCTGCAACGCCAACGGCTTCATCTGAAGCCAGTTGGAACAGCCAGACTGGATTGTTATCCCATCCTCCTGGTAAAGTTGCAGTTTCACTGCGGAGTCCTACTCATCATCATCATCAACATCATCATGATCACAAGAAAAGTAGAGGAATGGCCGCATCCCCATCGTCTTCGTCTTCTCCGAGAGTGAGATGGTTTAGTTTTCCAAGAAGATGTCCATGCTCTGGCAAGAAATCAGTTCGAGTTGAGGAAAAAATGTCATCACAGCAACCGGCTAAGACCTCATCATCACATTACACAGCAGCAGCAGCAGCCGCCCCGAGGACTGTATCCTTGGATCTCAGAAATCAAACACCCAAGTCAAAGTCAATGGTGGCAGCGAGTGGCGACCAGCATTTGATGTGGGATGAAGAACATCGTCGTCGTTGGGAGGGGCAGATGACTGTTAAGATACCATCAGCTGGAAACCCCCATAATCGATTATTCAGACCTCTTCCTGCTGCTGCGGAGATGCAAGCACAACATACTAACGCCTTTAGCTTTCCCATATTGAAACTGAATGGGAGGGGCACATCGTGGTCCTCGTCGTCACCAATGTTGAAGTTGCAGCAGCAGCCTGCTACTGTAAAACAAGAACCAACTCTGGGACGGGAAGGTCTGGAACCCTTGACTACTAGTACTGATGAACAATCATCCACAACTATTTCAATCCACAAGCCTATTACTACTAGTACTGATCACTCGAGTACTGGTTTCACATTCCCTGCAAGCCCCAAATCCCGAGTAATTGTAAGCAATGATGACGATTTGGGAAGCGATGCAAGCTCTGATTTATTCGAGATTGAAAGCTTCTCTACTAGTACTCAAACAACAGCTACTGCCAACAACTACCCATCAGTGTTGTACCAATACAACAACTACCGCAGGGATTCAATGGAAGATGATGCCTCCAGCTTTAATGCTATTAGAGGTTTGTATCGCCGGCGAAGCAGCTTAGATAATGAGCCAATGACGACTTCATCAGCCGAGTACTGCTACGAGCCTAGTGAAGCAAGCATAGACTGGAGTGTAACCACCGCAGAGGGGTTGGACCGGCCCAGCACATCATCGGAAGCAGATTACCATCAAGATGAGCTGGAAAAGATGAGCAGACGACGCTCGTCATCCGCAGGGAGTTATGGGTTGTTGAGCTGTCGATGGGAGAAGGCAGTGAGCGTGGGGCCGAACCCAGTGAGACTAGTGAGTACTATGCCTGCTGGTGCTAGTGCTGGTGCTGTTGGTGAGGTGATGAAGCATGTGGGGAGTAGGCCACCCTTACCCTATAAACCACGCTGCTCTCACTTGTCTATTCCTTCTGCAACATCATTCCATTCATAG

Protein Analysis

596

Amino Acids

65.24

Weight (kDa)

8.88

Isoelectric Point (pI)

61.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04190
fragaria_vesca FvH4_4g10470
malus_domestica MD12G1110900.v1.1
prunus_persica Prupe.7G023100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0495011
rosa_laevigata RLG00000022656
rosa_multiflora Rmu_co8188214.1_g000001 Rmu_ssc0000058.1_g000001
rosa_roxburghii Rroxscaffold_4G00319360 Rroxscaffold_6G00390850
rosa_rugosa Rorug03G0269300
rosa_samantha Rh3BG353200 Rh3CG313700 Rh3CG350100 Rh3CG350300 Rh3DG352600
rosa_wichuraiana Rw3G027740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 131
AasI GACNNNNNNGTC 1 cut(s) 1068
Acc36I ACCTGC 1 cut(s) 131
AccB7I CCANNNNNTGG 2 cut(s) 638, 875
AclWI GGATC 1 cut(s) 759
AcoI YGGCCR 1 cut(s) 567
AcuI CTGAAG 1 cut(s) 462
AdeI CACNNNGTG 2 cut(s) 217, 990
AfaI GTAC 9 cut(s) 68, 185, 1093, 1147, 1162, 1276, 1319, 1445, 1660
AfiI CCNNNNNNNGG 5 cut(s) 638, 735, 875, 1058, 1497
AgsI TTSAA 6 cut(s) 190, 967, 1015, 1122, 1259, 1347
AhlI ACTAGT 4 cut(s) 1088, 1142, 1271, 1651
AjnI CCWGG 1 cut(s) 484
AjuI GAANNNNNNNTTGG 2 cut(s) 1414, 1446
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 2 cut(s) 72, 1642
AlwI GGATC 1 cut(s) 759
AlwNI CAGNNNCTG 1 cut(s) 1292
Ama87I CYCGRG 3 cut(s) 733, 1156, 1194
AoxI GGCC 6 cut(s) 130, 174, 567, 1508, 1634, 1721
AseI ATTAAT 2 cut(s) 246, 255
Asp700I GAANNNNTTC 1 cut(s) 185
AspS9I GGNCC 5 cut(s) 175, 993, 1504, 1509, 1634
AsuHPI GGTGA 5 cut(s) 155, 229, 996, 1706, 1711
AvaI CYCGRG 3 cut(s) 733, 1156, 1194
AvaII GGWCC 2 cut(s) 993, 1504
BaeGI GKGCMC 1 cut(s) 986
BanII GRGCYC 1 cut(s) 403
BauI CACGAG 2 cut(s) 276, 331
BbsI GAAGAC 2 cut(s) 576, 582
Bbv12I GWGCWC 1 cut(s) 403
BccI CCATC 8 cut(s) 122, 486, 586, 600, 877, 1315, 1545, 1605
BceAI ACGGC 1 cut(s) 447
BcgI CGANNNNNNTGC 2 cut(s) 561, 595
BciT130I CCWGG 1 cut(s) 486
BciVI GTATCC 1 cut(s) 754
BclI TGATCA 2 cut(s) 544, 1150
BcoDI GTCTC 2 cut(s) 72, 1642
BcuI ACTAGT 4 cut(s) 1088, 1142, 1271, 1651
BfaI CTAG 6 cut(s) 1089, 1143, 1272, 1458, 1652, 1677
BfmI CTRYAG 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 131
BfuI GTATCC 1 cut(s) 754
BmcAI AGTACT 7 cut(s) 185, 1093, 1147, 1162, 1276, 1445, 1660
Bme1390I CCNGG 1 cut(s) 486
Bme18I GGWCC 2 cut(s) 993, 1504
BmeT110I CYCGRG 3 cut(s) 733, 1156, 1194
BmgT120I GGNCC 5 cut(s) 175, 993, 1504, 1509, 1634
BmiI GGNNCC 2 cut(s) 1077, 1635
BmrFI CCNGG 1 cut(s) 486
BmrI ACTGGG 1 cut(s) 1637
BmsI GCATC 7 cut(s) 196, 207, 309, 581, 915, 1222, 1348
BmuI ACTGGG 1 cut(s) 1637
BoxI GACNNNNGTC 1 cut(s) 1573
BpiI GAAGAC 2 cut(s) 576, 582
BplI GAGNNNNNCTC 2 cut(s) 502, 534
BpmI CTGGAG 3 cut(s) 352, 1348, 1498
Bsa29I ATCGAT 1 cut(s) 892
BsaBI GATNNNNATC 2 cut(s) 472, 1536
BsaJI CCNNGG 3 cut(s) 73, 734, 747
Bsc4I CCNNNNNNNGG 5 cut(s) 638, 735, 875, 1058, 1497
Bse118I RCCGGY 3 cut(s) 685, 1395, 1506
Bse1I ACTGG 6 cut(s) 369, 447, 469, 1168, 1481, 1643
Bse3DI GCAATG 1 cut(s) 1216
Bse8I GATNNNNATC 2 cut(s) 472, 1536
BseBI CCWGG 1 cut(s) 486
BseCI ATCGAT 1 cut(s) 892
BseDI CCNNGG 3 cut(s) 73, 734, 747
BseGI GGATG 8 cut(s) 69, 133, 347, 478, 572, 826, 1106, 1577
BseJI GATNNNNATC 2 cut(s) 472, 1536
BseLI CCNNNNNNNGG 5 cut(s) 638, 735, 875, 1058, 1497
BseMI GCAATG 1 cut(s) 1216
BseMII CTCAG 2 cut(s) 71, 769
BseNI ACTGG 6 cut(s) 369, 447, 469, 1168, 1481, 1643
BseSI GKGCMC 1 cut(s) 986
BseYI CCCAGC 1 cut(s) 1511
BshFI GGCC 6 cut(s) 132, 176, 569, 1510, 1636, 1723
BshVI ATCGAT 1 cut(s) 892
BsiHKAI GWGCWC 1 cut(s) 403
BsiHKCI CYCGRG 3 cut(s) 733, 1156, 1194
BsiSI CCGG 3 cut(s) 686, 1396, 1507
BslFI GGGAC 1 cut(s) 1074
BslI CCNNNNNNNGG 5 cut(s) 638, 735, 875, 1058, 1497
BsmAI GTCTC 2 cut(s) 72, 1642
BsmFI GGGAC 1 cut(s) 1074
BsnI GGCC 6 cut(s) 132, 176, 569, 1510, 1636, 1723
BsoBI CYCGRG 3 cut(s) 733, 1156, 1194
Bsp1286I GDGCHC 2 cut(s) 403, 986
Bsp143I GATC 5 cut(s) 8, 115, 544, 751, 1150
BspANI GGCC 6 cut(s) 132, 176, 569, 1510, 1636, 1723
BspCNI CTCAG 2 cut(s) 70, 768
BspDI ATCGAT 1 cut(s) 892
BspHI TCATGA 2 cut(s) 355, 541
BspLI GGNNCC 2 cut(s) 1077, 1635
BspMI ACCTGC 1 cut(s) 131
BspPI GGATC 1 cut(s) 759
BsrDI GCAATG 1 cut(s) 1216
BsrFI RCCGGY 3 cut(s) 685, 1395, 1506
BsrI ACTGG 6 cut(s) 369, 447, 469, 1168, 1481, 1643
BssAI RCCGGY 3 cut(s) 685, 1395, 1506
BssECI CCNNGG 3 cut(s) 73, 734, 747
BssMI GATC 5 cut(s) 8, 115, 544, 751, 1150
BssSI CACGAG 2 cut(s) 276, 331
BssT1I CCWWGG 2 cut(s) 73, 747
Bst2BI CACGAG 2 cut(s) 276, 331
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 3 cut(s) 742, 859, 1039
Bst6I CTCTTC 1 cut(s) 912
BstC8I GCNNGC 7 cut(s) 930, 1032, 1183, 1237, 1397, 1468, 1668
BstDEI CTNAG 4 cut(s) 57, 690, 755, 1408
BstF5I GGATG 8 cut(s) 69, 133, 347, 478, 572, 826, 1106, 1577
BstKTI GATC 5 cut(s) 11, 118, 547, 754, 1153
BstMAI GTCTC 2 cut(s) 72, 1642
BstMBI GATC 5 cut(s) 8, 115, 544, 751, 1150
BstNI CCWGG 1 cut(s) 486
BstNSI RCATGY 1 cut(s) 1712
BstPAI GACNNNNGTC 1 cut(s) 1573
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 1 cut(s) 986
BstV2I GAAGAC 2 cut(s) 576, 582
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 485
BstYI RGATCY 1 cut(s) 751
Bsu15I ATCGAT 1 cut(s) 892
BsuI GTATCC 1 cut(s) 754
BsuRI GGCC 6 cut(s) 132, 176, 569, 1510, 1636, 1723
BsuTUI ATCGAT 1 cut(s) 892
BtgZI GCGATG 1 cut(s) 1245
BtsCI GGATG 8 cut(s) 69, 133, 347, 478, 572, 826, 1106, 1577
BtsI GCAGTG 3 cut(s) 31, 503, 1629
BtsIMutI CAGTG 6 cut(s) 31, 153, 503, 1317, 1629, 1650
BveI ACCTGC 1 cut(s) 131
Cac8I GCNNGC 7 cut(s) 930, 1032, 1183, 1237, 1397, 1468, 1668
CaiI CAGNNNCTG 1 cut(s) 1292
CciI TCATGA 2 cut(s) 355, 541
Cfr10I RCCGGY 3 cut(s) 685, 1395, 1506
Cfr13I GGNCC 5 cut(s) 175, 993, 1504, 1509, 1634
ClaI ATCGAT 1 cut(s) 892
CseI GACGC 1 cut(s) 1578
Csp6I GTAC 9 cut(s) 67, 184, 1092, 1146, 1161, 1275, 1318, 1444, 1659
CviAII CATG 7 cut(s) 310, 356, 379, 413, 542, 633, 1709
CviQI GTAC 9 cut(s) 67, 184, 1092, 1146, 1161, 1275, 1318, 1444, 1659
DdeI CTNAG 4 cut(s) 57, 690, 755, 1408
DpnI GATC 5 cut(s) 10, 117, 546, 753, 1152
DpnII GATC 5 cut(s) 8, 115, 544, 751, 1150
DraIII CACNNNGTG 2 cut(s) 217, 990
DrdI GACNNNNNNGTC 1 cut(s) 1068
DseDI GACNNNNNNGTC 1 cut(s) 1068
EaeI YGGCCR 1 cut(s) 567
Eam1104I CTCTTC 1 cut(s) 912
EarI CTCTTC 1 cut(s) 912
Ecl136II GAGCTC 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 73, 747
Eco24I GRGCYC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 993, 1504
Eco53kI GAGCTC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 3 cut(s) 733, 1156, 1194
EcoICRI GAGCTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 2 cut(s) 73, 747
EcoT38I GRGCYC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 73, 747
FaeI CATG 7 cut(s) 313, 359, 382, 416, 545, 636, 1712
FaqI GGGAC 1 cut(s) 1074
FatI CATG 7 cut(s) 309, 355, 378, 412, 541, 632, 1708
FbaI TGATCA 2 cut(s) 544, 1150
FokI GGATG 8 cut(s) 56, 140, 334, 465, 559, 833, 1093, 1564
FriOI GRGCYC 1 cut(s) 403
FspBI CTAG 6 cut(s) 1089, 1143, 1272, 1458, 1652, 1677
GsaI CCCAGC 1 cut(s) 1515
GsuI CTGGAG 3 cut(s) 352, 1348, 1498
HaeIII GGCC 6 cut(s) 132, 176, 569, 1510, 1636, 1723
HapII CCGG 3 cut(s) 686, 1396, 1507
HgaI GACGC 1 cut(s) 1578
Hin1II CATG 7 cut(s) 313, 359, 382, 416, 545, 636, 1712
HindIII AAGCTT 1 cut(s) 1261
HinfI GANTC 4 cut(s) 149, 327, 511, 1343
HpaII CCGG 3 cut(s) 686, 1396, 1507
HphI GGTGA 5 cut(s) 155, 229, 996, 1706, 1711
Hpy188I TCNGA 7 cut(s) 60, 442, 597, 758, 902, 1243, 1525
Hpy188III TCNNGA 8 cut(s) 119, 324, 356, 542, 1073, 1194, 1252, 1541
Hpy99I CGWCG 4 cut(s) 837, 840, 1003, 1572
HpyAV CCTTC 4 cut(s) 330, 1061, 1612, 1776
HpyCH4III ACNGT 3 cut(s) 742, 859, 1039
HpyCH4V TGCA 7 cut(s) 422, 497, 928, 1021, 1181, 1235, 1772
HpyF3I CTNAG 4 cut(s) 57, 690, 755, 1408
Hsp92II CATG 7 cut(s) 313, 359, 382, 416, 545, 636, 1712
KroI GCCGGC 1 cut(s) 1395
KroNI GCCGGC 1 cut(s) 1397
Ksp22I TGATCA 2 cut(s) 544, 1150
Kzo9I GATC 5 cut(s) 8, 115, 544, 751, 1150
LweI GCATC 7 cut(s) 196, 207, 309, 581, 915, 1222, 1348
MaeI CTAG 6 cut(s) 1089, 1143, 1272, 1458, 1652, 1677
MaeIII GTNAC 2 cut(s) 1002, 1483
MalI GATC 5 cut(s) 10, 117, 546, 753, 1152
MboI GATC 5 cut(s) 8, 115, 544, 751, 1150
MboII GAAGA 7 cut(s) 264, 576, 582, 636, 836, 899, 1364
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 2 cut(s) 403, 986
MluCI AATT 1 cut(s) 1200
MlyI GAGTC 2 cut(s) 143, 520
MmeI TCCRAC 2 cut(s) 431, 1482
MreI CGCCGGCG 1 cut(s) 1395
MroNI GCCGGC 1 cut(s) 1395
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 5 cut(s) 246, 255, 375, 861, 1371
MslI CAYNNNNRTG 2 cut(s) 540, 1542
MspA1I CMGCKG 1 cut(s) 875
MspI CCGG 3 cut(s) 686, 1396, 1507
MspR9I CCNGG 1 cut(s) 486
MvaI CCWGG 1 cut(s) 486
NaeI GCCGGC 1 cut(s) 1397
NdeII GATC 5 cut(s) 8, 115, 544, 751, 1150
NgoMIV GCCGGC 1 cut(s) 1395
NlaIII CATG 7 cut(s) 313, 359, 382, 416, 545, 636, 1712
NlaIV GGNNCC 2 cut(s) 1077, 1635
NmeAIII GCCGAG 1 cut(s) 1465
NmuCI GTSAC 1 cut(s) 1002
NspI RCATGY 1 cut(s) 1712
PaeR7I CTCGAG 1 cut(s) 1156
PagI TCATGA 2 cut(s) 355, 541
PaqCI CACCTGC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 3 cut(s) 329, 593, 995
PdiI GCCGGC 1 cut(s) 1397
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 2 cut(s) 327, 1343
PflMI CCANNNNNTGG 2 cut(s) 638, 875
PleI GAGTC 2 cut(s) 143, 519
PpsI GAGTC 2 cut(s) 143, 519
PshAI GACNNNNGTC 1 cut(s) 1573
PshBI ATTAAT 2 cut(s) 246, 255
Psp124BI GAGCTC 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 484
PspFI CCCAGC 1 cut(s) 1511
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 2 cut(s) 1077, 1635
PspPI GGNCC 5 cut(s) 175, 993, 1504, 1509, 1634
PspXI VCTCGAGB 1 cut(s) 1156
PstNI CAGNNNCTG 1 cut(s) 1292
PsuI RGATCY 1 cut(s) 751
PvuII CAGCTG 1 cut(s) 875
RsaI GTAC 9 cut(s) 68, 185, 1093, 1147, 1162, 1276, 1319, 1445, 1660
RsaNI GTAC 9 cut(s) 67, 184, 1092, 1146, 1161, 1275, 1318, 1444, 1659
RseI CAYNNNNRTG 2 cut(s) 540, 1542
SacI GAGCTC 1 cut(s) 403
SaqAI TTAA 5 cut(s) 246, 255, 375, 861, 1371
Sau3AI GATC 5 cut(s) 8, 115, 544, 751, 1150
Sau96I GGNCC 5 cut(s) 175, 993, 1504, 1509, 1634
ScaI AGTACT 7 cut(s) 185, 1093, 1147, 1162, 1276, 1445, 1660
SchI GAGTC 2 cut(s) 143, 520
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 2 cut(s) 403, 986
SfaNI GCATC 7 cut(s) 196, 207, 309, 581, 915, 1222, 1348
SfcI CTRYAG 1 cut(s) 394
Sfr274I CTCGAG 1 cut(s) 1156
SgrAI CRCCGGYG 1 cut(s) 1395
SinI GGWCC 2 cut(s) 993, 1504
SlaI CTCGAG 1 cut(s) 1156
SmiMI CAYNNNNRTG 2 cut(s) 540, 1542
SmlI CTYRAG 1 cut(s) 1156
SmoI CTYRAG 1 cut(s) 1156
SpeI ACTAGT 4 cut(s) 1088, 1142, 1271, 1651
Sse9I AATT 1 cut(s) 1200
SspMI CTAG 6 cut(s) 1089, 1143, 1272, 1458, 1652, 1677
SstI GAGCTC 1 cut(s) 403
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 2 cut(s) 73, 747
TaaI ACNGT 3 cut(s) 742, 859, 1039
TaqI TCGA 7 cut(s) 303, 323, 655, 892, 1157, 1251, 1609
TaqII GACCGA 1 cut(s) 393
TasI AATT 1 cut(s) 1200
TatI WGTACW 8 cut(s) 66, 183, 1091, 1145, 1160, 1274, 1443, 1658
TauI GCSGC 6 cut(s) 33, 140, 341, 419, 572, 731
TfiI GAWTC 2 cut(s) 327, 1343
Tru1I TTAA 5 cut(s) 246, 255, 375, 861, 1371
Tru9I TTAA 5 cut(s) 246, 255, 375, 861, 1371
TscAI CASTG 6 cut(s) 31, 160, 510, 1317, 1629, 1650
TseFI GTSAC 1 cut(s) 1002
Tsp45I GTSAC 1 cut(s) 1002
TspDTI ATGAA 9 cut(s) 344, 375, 401, 426, 837, 1113, 1422, 1718, 1776
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 6 cut(s) 31, 160, 510, 1317, 1629, 1650
Van91I CCANNNNNTGG 2 cut(s) 638, 875
VpaK11BI GGWCC 2 cut(s) 993, 1504
VspI ATTAAT 2 cut(s) 246, 255
XceI RCATGY 1 cut(s) 1712
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XhoI CTCGAG 1 cut(s) 1156
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 6 cut(s) 1089, 1143, 1272, 1458, 1652, 1677
ZrmI AGTACT 7 cut(s) 185, 1093, 1147, 1162, 1276, 1445, 1660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.