MD12G1111100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011346
Physical Location & Seq
Forward (+)
17595668 .. 17598064
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1111100.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCAAACCCTCGAAGGAACACCCAGAGCTCAGAGAACCCCTTCCACGCCCAAACCCTCACATCCTCTTCAGTCACTCACTTTCTCAAGAAACCCCACGCCTTCCCCTTCCTCCTCTCAATCTTCCTTTTCCTGACGTGGGTCTCTCTCCGGCTCCAGCACTCCTCTTCTCCTTCTCGTTTTTCCATCAACAGAGACGACGCCCATAAAAAATGGAGCCAATCGAGCGATTCCAGAGCCAATCTCGTGAGGTTCGGTTCTGGGTTTCCTTCCCCGATTGCCAAAGACAAGAGGGGATGGTTCCTCGACCCCATCTCGCTCGCCAAAGATTCTCGCATTTCAGGTGGAGCTCTGAGCTGTGTATCGCTTCATCTCGGAGAAATCCGGCCTGGCGGTTTAAGGGGGAATCACAGGCACCATTCTTGCAATGAAACATTTGTCATATGGGGGGCTGAAACAAGGTTTAGGCTGGAGAATGACCAGGTGGATGAAGGCTATGCTGAAGTGACTATTGGTGCAGATGAGGTTGCTGTAGCAGCTAGCCCAAGTAGCACTGCCCATGCTCTAATAAATATAGATCCGGTACGGACTACATACTTTATAGGATGCCAAGACAGAATCATAAACTATAACAGCTCAAGTACAGATTTTAATGTGTGGAAAAATCTGAGTATAAGTTTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

25.42

Weight (kDa)

9.12

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56820 AT3G56820 AT3G56820
fragaria_vesca FvH4_1g29600 FvH4_1g29600 FvH4_1g29600 FvH4_1g29600
malus_domestica MD12G1111100.v1.1
prunus_persica Prupe.7G023500_v2.0.a1 Prupe.7G023500_v2.0.a1
pyrus_communis pycom12g09330
rosa_chinensis RchiOBHm_Chr7g0183121
rosa_laevigata RLG00000005077
rosa_multiflora Rmu_ssc0000111.1_g000009
rosa_roxburghii Rroxscaffold_3G00270740
rosa_rugosa Rorug06G0453300
rosa_samantha Rh7AG056300 Rh7BG055600 Rh7DG055800
rosa_wichuraiana Rw7G004590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 414
AciI CCGC 1 cut(s) 393
AclWI GGATC 1 cut(s) 572
AcuI CTGAAG 2 cut(s) 54, 522
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 2 cut(s) 585, 643
AfiI CCNNNNNNNGG 1 cut(s) 139
AjiI CACGTC 1 cut(s) 138
AjnI CCWGG 2 cut(s) 388, 480
AjuI GAANNNNNNNTTGG 2 cut(s) 495, 527
AluBI AGCT 5 cut(s) 30, 350, 357, 539, 636
AluI AGCT 5 cut(s) 30, 350, 357, 539, 636
Alw21I GWGCWC 2 cut(s) 32, 352
Alw26I GTCTC 2 cut(s) 148, 189
AlwI GGATC 1 cut(s) 572
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 41
AsuNHI GCTAGC 1 cut(s) 539
BanI GGYRCC 1 cut(s) 414
BanII GRGCYC 2 cut(s) 32, 352
BauI CACGAG 1 cut(s) 245
Bbv12I GWGCWC 2 cut(s) 32, 352
BbvI GCAGC 1 cut(s) 548
BccI CCATC 3 cut(s) 194, 291, 320
BciT130I CCWGG 2 cut(s) 390, 482
BcoDI GTCTC 2 cut(s) 148, 189
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 531
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
Bme1390I CCNGG 2 cut(s) 390, 482
BmgBI CACGTC 1 cut(s) 138
BmiI GGNNCC 4 cut(s) 155, 218, 302, 416
BmrFI CCNGG 2 cut(s) 390, 482
BmsI GCATC 1 cut(s) 596
BmtI GCTAGC 1 cut(s) 543
BoxI GACNNNNGTC 1 cut(s) 140
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BpmI CTGGAG 2 cut(s) 140, 491
BpuEI CTTGAG 2 cut(s) 71, 622
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 148
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 433
BseBI CCWGG 2 cut(s) 390, 482
BseGI GGATG 4 cut(s) 62, 302, 493, 611
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 3 cut(s) 45, 344, 659
BseRI GAGGAG 2 cut(s) 104, 154
BseXI GCAGC 1 cut(s) 548
BsgI GTGCAG 1 cut(s) 537
BshFI GGCC 1 cut(s) 388
BshNI GGYRCC 1 cut(s) 414
BsiHKAI GWGCWC 2 cut(s) 32, 352
BsiSI CCGG 3 cut(s) 151, 385, 581
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 2 cut(s) 148, 189
BsmBI CGTCTC 1 cut(s) 189
BsnI GGCC 1 cut(s) 388
Bso31I GGTCTC 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 32, 352
Bsp143I GATC 1 cut(s) 577
BspACI CCGC 1 cut(s) 393
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 3 cut(s) 44, 345, 660
BspLI GGNNCC 4 cut(s) 155, 218, 302, 416
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 1 cut(s) 572
BspT107I GGYRCC 1 cut(s) 414
BspTNI GGTCTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 433
BssMI GATC 1 cut(s) 577
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 245
Bst2UI CCWGG 2 cut(s) 390, 482
Bst6I CTCTTC 2 cut(s) 73, 172
BstACI GRCGYC 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 321, 541
BstDEI CTNAG 3 cut(s) 31, 353, 668
BstF5I GGATG 4 cut(s) 62, 302, 493, 611
BstKTI GATC 1 cut(s) 580
BstMAI GTCTC 2 cut(s) 148, 189
BstMBI GATC 1 cut(s) 577
BstMWI GCNNNNNNNGC 3 cut(s) 225, 536, 549
BstNI CCWGG 2 cut(s) 390, 482
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 388, 480
BstSFI CTRYAG 1 cut(s) 531
BstV1I GCAGC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuRI GGCC 1 cut(s) 388
BtrI CACGTC 1 cut(s) 138
BtsCI GGATG 4 cut(s) 62, 302, 493, 611
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 2 cut(s) 321, 541
CseI GACGC 1 cut(s) 209
CsiI ACCWGGT 1 cut(s) 480
Csp6I GTAC 2 cut(s) 584, 642
CviAII CATG 1 cut(s) 560
CviQI GTAC 2 cut(s) 584, 642
DdeI CTNAG 3 cut(s) 31, 353, 668
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
Eam1104I CTCTTC 2 cut(s) 73, 172
EarI CTCTTC 2 cut(s) 73, 172
Ecl136II GAGCTC 2 cut(s) 30, 350
Eco24I GRGCYC 2 cut(s) 32, 352
Eco31I GGTCTC 1 cut(s) 148
Eco53kI GAGCTC 2 cut(s) 30, 350
Eco57I CTGAAG 2 cut(s) 54, 522
EcoICRI GAGCTC 2 cut(s) 30, 350
EcoRII CCWGG 2 cut(s) 388, 480
EcoT38I GRGCYC 2 cut(s) 32, 352
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 1 cut(s) 563
FatI CATG 1 cut(s) 559
FauNDI CATATG 1 cut(s) 443
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 4 cut(s) 49, 309, 500, 618
FriOI GRGCYC 2 cut(s) 32, 352
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 1 cut(s) 540
GluI GCNGC 1 cut(s) 537
GsuI CTGGAG 2 cut(s) 140, 491
HaeIII GGCC 1 cut(s) 388
HapII CCGG 3 cut(s) 151, 385, 581
HgaI GACGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 1 cut(s) 563
HinfI GANTC 4 cut(s) 230, 329, 406, 618
HpaII CCGG 3 cut(s) 151, 385, 581
Hpy188I TCNGA 4 cut(s) 34, 354, 377, 669
Hpy188III TCNNGA 4 cut(s) 88, 133, 234, 247
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 7 cut(s) 9, 52, 112, 118, 183, 279, 485
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 2 cut(s) 426, 518
HpyF10VI GCNNNNNNNGC 3 cut(s) 225, 536, 549
HpyF3I CTNAG 3 cut(s) 31, 353, 668
HpySE526I ACGT 1 cut(s) 137
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 1 cut(s) 563
Kzo9I GATC 1 cut(s) 577
LmnI GCTCC 3 cut(s) 159, 216, 347
Lsp1109I GCAGC 1 cut(s) 548
LweI GCATC 1 cut(s) 596
MabI ACCWGGT 1 cut(s) 480
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 2 cut(s) 73, 505
MalI GATC 1 cut(s) 579
MboI GATC 1 cut(s) 577
MboII GAAGA 3 cut(s) 60, 115, 159
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 2 cut(s) 32, 352
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 2 cut(s) 398, 651
MspI CCGG 3 cut(s) 151, 385, 581
MspR9I CCNGG 2 cut(s) 390, 482
MvaI CCWGG 2 cut(s) 390, 482
MwoI GCNNNNNNNGC 3 cut(s) 225, 536, 549
NdeI CATATG 1 cut(s) 443
NdeII GATC 1 cut(s) 577
NheI GCTAGC 1 cut(s) 539
NlaIII CATG 1 cut(s) 563
NlaIV GGNNCC 4 cut(s) 155, 218, 302, 416
NmuCI GTSAC 2 cut(s) 73, 505
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 4 cut(s) 230, 329, 406, 618
PkrI GCNGC 1 cut(s) 538
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 2 cut(s) 32, 352
Psp6I CCWGG 2 cut(s) 388, 480
PspGI CCWGG 2 cut(s) 388, 480
PspN4I GGNNCC 4 cut(s) 155, 218, 302, 416
PsuI RGATCY 1 cut(s) 577
RsaI GTAC 2 cut(s) 585, 643
RsaNI GTAC 2 cut(s) 584, 642
SacI GAGCTC 2 cut(s) 32, 352
SaqAI TTAA 2 cut(s) 398, 651
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 1 cut(s) 577
ScrFI CCNGG 2 cut(s) 390, 482
SduI GDGCHC 2 cut(s) 32, 352
SexAI ACCWGGT 1 cut(s) 480
SfaNI GCATC 1 cut(s) 596
SfcI CTRYAG 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 86, 637
SmoI CTYRAG 2 cut(s) 86, 637
SsiI CCGC 1 cut(s) 393
SspMI CTAG 1 cut(s) 540
SstI GAGCTC 2 cut(s) 32, 352
StyD4I CCNGG 2 cut(s) 388, 480
TaiI ACGT 1 cut(s) 140
TaqI TCGA 3 cut(s) 13, 224, 306
TatI WGTACW 1 cut(s) 641
TfiI GAWTC 4 cut(s) 230, 329, 406, 618
Tru1I TTAA 2 cut(s) 398, 651
Tru9I TTAA 2 cut(s) 398, 651
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 2 cut(s) 73, 505
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 2 cut(s) 73, 505
TspDTI ATGAA 3 cut(s) 359, 444, 504
TspGWI ACGGA 1 cut(s) 601
TspRI CASTG 1 cut(s) 559
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.