MD12G1125800.v1.1

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014760
Physical Location & Seq
Forward (+)
20109685 .. 20115277
5593 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1125800.v1.1.491

Sequence Viewer

Length: 2379 bp
ATGGATTTGACGGATCAGCAGCTGAAGCCGTGCAAATGCGGTTATGAGGTATGTGTTTGGTGTTGGCATCACATTATGGAAATGGCAGCGAAGGATGGAAAGGAGGGTCGCTGTCCAGTATGCCGTACACCTTATGACAAAGAAAAAATTGTTGGAGTGGCGGCAAATTGTCAAAAGTTGGTGGCTGAAATTAATGTGAAGCGGAAACAGAAAAAACCCAAGGGATCTGAAGTAAAGAAGCAGCATCTTACTGATGTTAGAGTTCTCCAACGGACCCTAGTATATGTGATTGGAATACCTCTTAACCTAGCGAGTGAAGACCTTCTCAAGCGAAGGGAGTATTTTGGCCAGTACGGAAAGGTTTTGAAGGTTTCTATCTCTCGTACAGCAAATGGGGCTATCCAACAGGCTCCAAACAACAGTTGTTGTGTGTATATATCATACTCAAAAGAATATGAAGCAGCTCGGTGCATTCAATCAGTACACAGTTTTGTGTTAGATGGTCGATGCTTGAGGGCATCTTTTGGAACCACAAAGTACTGCCATGCATGGTTGAAAAGTGTGCCTTGCAACAATCCAGATTGTTGGTATTTGCATGATTTTGGTTCTCAGGAAGATAGTTTTACCAAGGATGAATTGCTTTCAGCCTTTGAAAGGAGTAAAGATCACCAAAATATCGGTGTTACAAATAATTTGAATCGGCATTCAGTGAAAGGCTTACCTCCCCCAACTGCTGAGAATAGCAAAGCTGAAAGATCATCAGCTACTAAACATCCATCAAATATTACTGGGAATGAGGTTAAGAGTTATCGTGCAGATGGTGACTCCAGAACATCTATTCCTGCAATAGCACCTTGGGTCATGCAAGTCACCCCTAGTGTGCCCCCAGCAACAAGTTCTCCAGGTTCTGAAAGGGCTCTTTATCAAAATCCTGAGAAATCAAGTGATCTCAATCCCCTTTCATCAGAAGTGGTACGCAATGAAAGTTCTACCCACAATGTAAGATCTGAAGAAAGCTGTGAGTGGCTAATGACCGAACAGGTTATCACCAAAAATGGTCAAGCAGCTATATCAAATACAACTTCTGGTGCTCTAGCAGAAAGAGCTCCGGTCAGTGTAACTTCCAACAAAAACTTATGTTGTCCATCACCAAACAATGGCATTGAGCGGGGTAGCAGAGTAACATCCACCAACTTGATCAGCAACAACAACAGTTATTGTTCATCATCAAACTTATCAGATAAATCCATTGATATCCAAGGCAGTAGTGTTGCTGAATTGTATAGGACATATGAACCTACCAGTGTGTTACCTGAGATGGGATTAGGGAAGTACTCGAGAGGGTGTGAAATCAATAAGCCCAGCATTCACCGCAGTCTTTCTCCAGATATAGGAGAGAGTGGCATAATCTCAAGTATTTTGACTATGGACTTGGATGCACCTGACGGTTCTTTATCTCCTCTTCACAATCTTGTAAAGTTGTTGGGTGAAACTGATAAAGAGTGCAGTCTCCTCAGAGTACAAAATTCACGAAAACTGCTTGATAAAAAGCAGTCCAGGTTCTCATTTGCTCAACAAGAGGATTCTTGTGATAATAATATATGGAATGCATCCAAGGACTACGCCTTATCTGATTTGATGGGCAACAAGGAGTACTTAGTAGATGTAGAGTCTAATAAGTTTCTTGGGGTTCCTTCTTTTACATCTTCTAAAGTATCAGAAGTTCCTCGTTCAATTGCGCCAGGATTTTCAATGCCAAGTAGAGCACCACCTCCAGGGTTTTCTTCTCCTCCAGGGTTTTCTTCTCATTTGAGGCCTAGGAGGGTGGATCAGGCTTTTGACAGTTCAGTGAATCACTTGTTGCAACCATCATGTATACCAACTGGTTATTCTGGTATTAATGGGAATGTCGAGATCAATGATCCAGCAATTTTGGAAGTCAGTGAGGAACTATTGGCAAGAGGGTTGATGAATAAGAGAGCAAATTTATCTCAGTTTAACTCTTCTGAGCCAGATGCTAGGCTCCATATGCTGAGACAGCAGACAACTTCTGCCCCGCAGAACTTAGGATGTCAAGACCACTTCATGAATAGTTACTCTTACCTAAATGATGCACAAAGAATTACTCCACAGCAGCCAGATCAATTCCAGCCCAATCCATCATTTGTGCAACCCACCAGTCAACACTTAAGTGACATGCATTTATCAAATAATGATTGGGTGCAATGGAATCGGGTCAATAGTGTTGCCGATCTTGGTATATCAGATCTACTGAGAAACGATCGACTGGGATTTAACAAGCTCATTCCCTGTTATGAGAATATGCAGTCTCCAACAAGTTACTTCAGTCATCTAAACAACAGATCATTTGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
RRM_1 zf-RING_4

Protein Analysis

793

Amino Acids

87.88

Weight (kDa)

7.48

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 1 - 46 2.7e-15 RING/Ubox like zinc-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g15190
malus_domestica MD12G1125800.v1.1
prunus_persica Prupe.6G242300_v2.0.a1 Prupe.6G242300_v2.0.a1
pyrus_communis pycom12g12400
rosa_chinensis RchiOBHm_Chr3g0468541
rosa_laevigata RLG00000024424
rosa_multiflora Rmu_sc0008735.1_g000001 Rmu_sc0009516.1_g000009
rosa_roxburghii Rroxscaffold_6G00412710
rosa_rugosa Rorug03G0095700 Rorug03G0095800 Rorug03G0095800
rosa_samantha Rh3AG144200 Rh3BG166400 Rh3DG165800
rosa_wichuraiana Rw3G013610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1157
AccBSI CCGCTC 1 cut(s) 1168
AccI GTMKAC 1 cut(s) 1876
AciI CCGC 6 cut(s) 39, 161, 202, 1168, 1372, 2057
AclWI GGATC 4 cut(s) 21, 232, 1836, 1916
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 2 cut(s) 1525, 1984
AcuI CTGAAG 4 cut(s) 44, 249, 1029, 2329
AfaI GTAC 9 cut(s) 127, 353, 385, 483, 539, 975, 1334, 1521, 1655
AfiI CCNNNNNNNGG 5 cut(s) 654, 1157, 1319, 1391, 1775
AflII CTTAAG 1 cut(s) 2188
AgsI TTSAA 8 cut(s) 367, 476, 556, 653, 697, 1734, 1752, 2372
AjnI CCWGG 5 cut(s) 901, 1556, 1741, 1774, 1792
AjuI GAANNNNNNNTTGG 4 cut(s) 135, 167, 1873, 1905
AleI CACNNNNGTG 1 cut(s) 2190
AloI GAACNNNNNNTCC 2 cut(s) 823, 855
AluBI AGCT 8 cut(s) 22, 464, 749, 764, 1017, 1067, 1106, 2302
AluI AGCT 8 cut(s) 22, 464, 749, 764, 1017, 1067, 1106, 2302
Alw21I GWGCWC 3 cut(s) 1093, 1108, 1768
Alw26I GTCTC 3 cut(s) 1514, 2029, 2334
AlwI GGATC 4 cut(s) 21, 232, 1836, 1916
AlwNI CAGNNNCTG 2 cut(s) 22, 908
Ama87I CYCGRG 1 cut(s) 1336
AoxI GGCC 2 cut(s) 346, 1814
ApeKI GCWGC 6 cut(s) 19, 86, 241, 461, 1064, 2134
ApoI RAATTY 2 cut(s) 1525, 1984
AseI ATTAAT 2 cut(s) 192, 1899
Asp700I GAANNNNTTC 1 cut(s) 321
AspA2I CCTAGG 1 cut(s) 1817
AspLEI GCGC 1 cut(s) 1741
AspS9I GGNCC 1 cut(s) 273
AsuHPI GGTGA 7 cut(s) 659, 833, 862, 1039, 1140, 1361, 1499
AvaI CYCGRG 1 cut(s) 1336
AvaII GGWCC 1 cut(s) 273
AvrII CCTAGG 1 cut(s) 1817
BaeGI GKGCMC 1 cut(s) 885
BalI TGGCCA 1 cut(s) 348
BanII GRGCYC 2 cut(s) 919, 1108
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 3 cut(s) 1093, 1108, 1768
BbvI GCAGC 6 cut(s) 31, 98, 253, 473, 1076, 2146
BccI CCATC 9 cut(s) 89, 494, 784, 812, 1153, 1312, 1633, 1876, 2167
BceAI ACGGC 2 cut(s) 13, 108
BcgI CGANNNNNNTGC 2 cut(s) 2213, 2247
BciT130I CCWGG 5 cut(s) 903, 1558, 1743, 1776, 1794
BclI TGATCA 1 cut(s) 1197
BcoDI GTCTC 3 cut(s) 1514, 2029, 2334
BfaI CTAG 6 cut(s) 278, 308, 876, 1094, 1818, 2019
BfrI CTTAAG 1 cut(s) 2188
BglII AGATCT 2 cut(s) 1004, 2266
BisI GCNGC 7 cut(s) 20, 87, 162, 242, 462, 1065, 2135
BlnI CCTAGG 1 cut(s) 1817
BlsI GCNGC 7 cut(s) 21, 88, 163, 243, 463, 1066, 2136
BmcAI AGTACT 3 cut(s) 539, 1334, 1655
Bme1390I CCNGG 5 cut(s) 903, 1558, 1743, 1776, 1794
Bme18I GGWCC 1 cut(s) 273
BmeT110I CYCGRG 1 cut(s) 1336
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 5 cut(s) 275, 411, 529, 1692, 2024
BmrFI CCNGG 5 cut(s) 903, 1558, 1743, 1776, 1794
BmrI ACTGGG 2 cut(s) 798, 2297
BmsI GCATC 8 cut(s) 76, 253, 497, 527, 1426, 1619, 2005, 2101
BmuI ACTGGG 2 cut(s) 798, 2297
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 5 cut(s) 811, 885, 1368, 1758, 1776
BpuEI CTTGAG 3 cut(s) 311, 532, 1396
BsaBI GATNNNNATC 1 cut(s) 951
BsaJI CCNNGG 8 cut(s) 219, 627, 854, 1258, 1614, 1775, 1793, 1817
BsaWI WCCGGW 1 cut(s) 1108
BsaXI ACNNNNNCTCC 2 cut(s) 883, 913
Bsc4I CCNNNNNNNGG 5 cut(s) 654, 1157, 1319, 1391, 1775
Bse1I ACTGG 7 cut(s) 116, 349, 793, 1302, 1888, 2178, 2292
Bse3DI GCAATG 2 cut(s) 985, 2231
Bse8I GATNNNNATC 1 cut(s) 951
BseBI CCWGG 5 cut(s) 903, 1558, 1743, 1776, 1794
BseDI CCNNGG 8 cut(s) 219, 627, 854, 1258, 1614, 1775, 1793, 1817
BseGI GGATG 7 cut(s) 100, 637, 772, 1184, 1441, 1610, 2075
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 5 cut(s) 654, 1157, 1319, 1391, 1775
BseMI GCAATG 2 cut(s) 985, 2231
BseMII CTCAG 9 cut(s) 623, 726, 924, 1305, 1528, 1998, 2006, 2024, 2264
BseNI ACTGG 7 cut(s) 116, 349, 793, 1302, 1888, 2178, 2292
BseRI GAGGAG 3 cut(s) 1449, 1502, 1779
BseSI GKGCMC 1 cut(s) 885
BseXI GCAGC 6 cut(s) 31, 98, 253, 473, 1076, 2146
BseYI CCCAGC 2 cut(s) 886, 1361
BsgI GTGCAG 2 cut(s) 834, 1525
Bsh1285I CGRYCG 1 cut(s) 2284
BshFI GGCC 2 cut(s) 348, 1816
BsiEI CGRYCG 1 cut(s) 2284
BsiHKAI GWGCWC 3 cut(s) 1093, 1108, 1768
BsiHKCI CYCGRG 1 cut(s) 1336
BsiSI CCGG 1 cut(s) 1109
BslI CCNNNNNNNGG 5 cut(s) 654, 1157, 1319, 1391, 1775
BsmAI GTCTC 3 cut(s) 1514, 2029, 2334
BsmI GAATGC 4 cut(s) 471, 703, 1365, 1612
BsnI GGCC 2 cut(s) 348, 1816
BsoBI CYCGRG 1 cut(s) 1336
Bsp1286I GDGCHC 5 cut(s) 885, 919, 1093, 1108, 1768
BspACI CCGC 6 cut(s) 39, 161, 202, 1168, 1372, 2057
BspANI GGCC 2 cut(s) 348, 1816
BspCNI CTCAG 9 cut(s) 622, 727, 925, 1306, 1527, 1999, 2005, 2025, 2265
BspHI TCATGA 1 cut(s) 2085
BspLI GGNNCC 5 cut(s) 275, 411, 529, 1692, 2024
BspPI GGATC 4 cut(s) 21, 232, 1836, 1916
BspTI CTTAAG 1 cut(s) 2188
BsrBI CCGCTC 1 cut(s) 1168
BsrDI GCAATG 2 cut(s) 985, 2231
BsrI ACTGG 7 cut(s) 116, 349, 793, 1302, 1888, 2178, 2292
BssECI CCNNGG 8 cut(s) 219, 627, 854, 1258, 1614, 1775, 1793, 1817
BssNAI GTATAC 1 cut(s) 1877
BssT1I CCWWGG 6 cut(s) 219, 627, 854, 1258, 1614, 1817
Bst1107I GTATAC 1 cut(s) 1877
Bst2UI CCWGG 5 cut(s) 903, 1558, 1743, 1776, 1794
Bst4CI ACNGT 5 cut(s) 422, 488, 1214, 1448, 1844
Bst6I CTCTTC 2 cut(s) 1467, 2008
BstAFI CTTAAG 1 cut(s) 2188
BstENI CCTNNNNNAGG 1 cut(s) 652
BstF5I GGATG 7 cut(s) 100, 637, 772, 1184, 1441, 1610, 2075
BstHHI GCGC 1 cut(s) 1741
BstMAI GTCTC 3 cut(s) 1514, 2029, 2334
BstMCI CGRYCG 1 cut(s) 2284
BstMWI GCNNNNNNNGC 6 cut(s) 25, 395, 1103, 1371, 2029, 2038
BstNI CCWGG 5 cut(s) 903, 1558, 1743, 1776, 1794
BstNSI RCATGY 1 cut(s) 2200
BstSCI CCNGG 5 cut(s) 901, 1556, 1741, 1774, 1792
BstSLI GKGCMC 1 cut(s) 885
BstV1I GCAGC 6 cut(s) 31, 98, 253, 473, 1076, 2146
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 3 cut(s) 224, 1004, 2266
BstXI CCANNNNNNTGG 1 cut(s) 585
BstYI RGATCY 3 cut(s) 224, 1004, 2266
BstZ17I GTATAC 1 cut(s) 1877
BsuRI GGCC 2 cut(s) 348, 1816
BtsCI GGATG 7 cut(s) 100, 637, 772, 1184, 1441, 1610, 2075
BtsIMutI CAGTG 5 cut(s) 714, 1120, 1309, 1854, 1948
CaiI CAGNNNCTG 2 cut(s) 22, 908
CciI TCATGA 1 cut(s) 2085
CfoI GCGC 1 cut(s) 1741
Cfr13I GGNCC 1 cut(s) 273
Csp6I GTAC 9 cut(s) 126, 352, 384, 482, 538, 974, 1333, 1520, 1654
CviAII CATG 7 cut(s) 545, 549, 596, 862, 1872, 2086, 2197
CviQI GTAC 9 cut(s) 126, 352, 384, 482, 538, 974, 1333, 1520, 1654
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 2 cut(s) 1467, 2008
EarI CTCTTC 2 cut(s) 1467, 2008
Ecl136II GAGCTC 1 cut(s) 1106
Eco130I CCWWGG 6 cut(s) 219, 627, 854, 1258, 1614, 1817
Eco147I AGGCCT 1 cut(s) 1816
Eco24I GRGCYC 2 cut(s) 919, 1108
Eco32I GATATC 1 cut(s) 1255
Eco47I GGWCC 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 1106
Eco57I CTGAAG 4 cut(s) 44, 249, 1029, 2329
Eco88I CYCGRG 1 cut(s) 1336
EcoICRI GAGCTC 1 cut(s) 1106
EcoNI CCTNNNNNAGG 1 cut(s) 652
EcoRII CCWGG 5 cut(s) 901, 1556, 1741, 1774, 1792
EcoRV GATATC 1 cut(s) 1255
EcoT14I CCWWGG 6 cut(s) 219, 627, 854, 1258, 1614, 1817
EcoT22I ATGCAT 3 cut(s) 550, 1612, 2202
EcoT38I GRGCYC 2 cut(s) 919, 1108
ErhI CCWWGG 6 cut(s) 219, 627, 854, 1258, 1614, 1817
FaeI CATG 7 cut(s) 548, 552, 599, 865, 1875, 2089, 2200
FalI AAGNNNNNCTT 4 cut(s) 550, 582, 1640, 1672
FatI CATG 7 cut(s) 544, 548, 595, 861, 1871, 2085, 2196
FauI CCCGC 2 cut(s) 1161, 2064
FauNDI CATATG 2 cut(s) 1291, 2028
FbaI TGATCA 1 cut(s) 1197
FblI GTMKAC 1 cut(s) 1876
Fnu4HI GCNGC 7 cut(s) 20, 87, 162, 242, 462, 1065, 2135
FokI GGATG 7 cut(s) 107, 644, 759, 1171, 1448, 1597, 2082
FriOI GRGCYC 2 cut(s) 919, 1108
Fsp4HI GCNGC 7 cut(s) 20, 87, 162, 242, 462, 1065, 2135
FspBI CTAG 6 cut(s) 278, 308, 876, 1094, 1818, 2019
GlaI GCGC 1 cut(s) 1740
GluI GCNGC 7 cut(s) 20, 87, 162, 242, 462, 1065, 2135
GsaI CCCAGC 2 cut(s) 890, 1365
GsuI CTGGAG 5 cut(s) 811, 885, 1368, 1758, 1776
HaeIII GGCC 2 cut(s) 348, 1816
HapII CCGG 1 cut(s) 1109
HhaI GCGC 1 cut(s) 1741
Hin1II CATG 7 cut(s) 548, 552, 599, 865, 1875, 2089, 2200
Hin6I GCGC 1 cut(s) 1739
HinP1I GCGC 1 cut(s) 1739
HincII GTYRAC 1 cut(s) 2183
HindII GTYRAC 1 cut(s) 2183
HinfI GANTC 6 cut(s) 697, 824, 1583, 1670, 1852, 2230
HpaII CCGG 1 cut(s) 1109
HphI GGTGA 7 cut(s) 659, 833, 862, 1039, 1140, 1361, 1499
Hpy166II GTNNAC 4 cut(s) 128, 484, 1877, 2183
Hpy8I GTNNAC 4 cut(s) 128, 484, 1877, 2183
HpyAV CCTTC 5 cut(s) 85, 327, 332, 361, 1704
HpyCH4III ACNGT 5 cut(s) 422, 488, 1214, 1448, 1844
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 395, 1103, 1371, 2029, 2038
Hsp92II CATG 7 cut(s) 548, 552, 599, 865, 1875, 2089, 2200
HspAI GCGC 1 cut(s) 1739
Ksp22I TGATCA 1 cut(s) 1197
LmnI GCTCC 3 cut(s) 415, 1111, 2028
Lsp1109I GCAGC 6 cut(s) 31, 98, 253, 473, 1076, 2146
LweI GCATC 8 cut(s) 76, 253, 497, 527, 1426, 1619, 2005, 2101
MaeI CTAG 6 cut(s) 278, 308, 876, 1094, 1818, 2019
MaeIII GTNAC 9 cut(s) 682, 821, 868, 1117, 1180, 1308, 2093, 2192, 2339
MbiI CCGCTC 1 cut(s) 1168
MboII GAAGA 8 cut(s) 329, 626, 1022, 1454, 1698, 1776, 1794, 1995
MfeI CAATTG 1 cut(s) 1734
MflI RGATCY 3 cut(s) 224, 1004, 2266
MhlI GDGCHC 5 cut(s) 885, 919, 1093, 1108, 1768
MlsI TGGCCA 1 cut(s) 348
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 2 cut(s) 818, 1679
MmeI TCCRAC 5 cut(s) 133, 292, 427, 1149, 2357
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 3 cut(s) 550, 1612, 2202
MroXI GAANNNNTTC 1 cut(s) 321
MscI TGGCCA 1 cut(s) 348
MseI TTAA 7 cut(s) 192, 303, 801, 1899, 1998, 2189, 2295
MslI CAYNNNNRTG 1 cut(s) 2190
Msp20I TGGCCA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 22
MspCI CTTAAG 1 cut(s) 2188
MspI CCGG 1 cut(s) 1109
MspR9I CCNGG 5 cut(s) 903, 1558, 1743, 1776, 1794
MunI CAATTG 1 cut(s) 1734
Mva1269I GAATGC 4 cut(s) 471, 703, 1365, 1612
MvaI CCWGG 5 cut(s) 903, 1558, 1743, 1776, 1794
MwoI GCNNNNNNNGC 6 cut(s) 25, 395, 1103, 1371, 2029, 2038
NdeI CATATG 2 cut(s) 1291, 2028
NlaIII CATG 7 cut(s) 548, 552, 599, 865, 1875, 2089, 2200
NlaIV GGNNCC 5 cut(s) 275, 411, 529, 1692, 2024
NmuCI GTSAC 3 cut(s) 821, 868, 2192
NsiI ATGCAT 3 cut(s) 550, 1612, 2202
NspI RCATGY 1 cut(s) 2200
OliI CACNNNNGTG 1 cut(s) 2190
PaeR7I CTCGAG 1 cut(s) 1336
PagI TCATGA 1 cut(s) 2085
PceI AGGCCT 1 cut(s) 1816
PctI GAATGC 4 cut(s) 471, 703, 1365, 1612
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 4 cut(s) 697, 1583, 1852, 2230
PflMI CCANNNNNTGG 1 cut(s) 1157
PkrI GCNGC 7 cut(s) 21, 88, 163, 243, 463, 1066, 2136
Ple19I CGATCG 1 cut(s) 2284
PleI GAGTC 2 cut(s) 818, 1678
PpsI GAGTC 2 cut(s) 818, 1678
PshBI ATTAAT 2 cut(s) 192, 1899
Psp124BI GAGCTC 1 cut(s) 1108
Psp6I CCWGG 5 cut(s) 901, 1556, 1741, 1774, 1792
PspFI CCCAGC 2 cut(s) 886, 1361
PspGI CCWGG 5 cut(s) 901, 1556, 1741, 1774, 1792
PspN4I GGNNCC 5 cut(s) 275, 411, 529, 1692, 2024
PspPI GGNCC 1 cut(s) 273
PsrI GAACNNNNNNTAC 2 cut(s) 1707, 1739
PstNI CAGNNNCTG 2 cut(s) 22, 908
PsuI RGATCY 3 cut(s) 224, 1004, 2266
PvuI CGATCG 1 cut(s) 2284
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 9 cut(s) 127, 353, 385, 483, 539, 975, 1334, 1521, 1655
RsaNI GTAC 9 cut(s) 126, 352, 384, 482, 538, 974, 1333, 1520, 1654
RseI CAYNNNNRTG 1 cut(s) 2190
SacI GAGCTC 1 cut(s) 1108
SaqAI TTAA 7 cut(s) 192, 303, 801, 1899, 1998, 2189, 2295
SatI GCNGC 7 cut(s) 20, 87, 162, 242, 462, 1065, 2135
Sau96I GGNCC 1 cut(s) 273
ScaI AGTACT 3 cut(s) 539, 1334, 1655
SchI GAGTC 2 cut(s) 818, 1679
ScrFI CCNGG 5 cut(s) 903, 1558, 1743, 1776, 1794
SduI GDGCHC 5 cut(s) 885, 919, 1093, 1108, 1768
SfaNI GCATC 8 cut(s) 76, 253, 497, 527, 1426, 1619, 2005, 2101
Sfr274I CTCGAG 1 cut(s) 1336
SinI GGWCC 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 1336
SmiMI CAYNNNNRTG 1 cut(s) 2190
SmlI CTYRAG 5 cut(s) 326, 511, 1336, 1411, 2188
SmoI CTYRAG 5 cut(s) 326, 511, 1336, 1411, 2188
SseBI AGGCCT 1 cut(s) 1816
SsiI CCGC 6 cut(s) 39, 161, 202, 1168, 1372, 2057
SspI AATATT 1 cut(s) 784
SspMI CTAG 6 cut(s) 278, 308, 876, 1094, 1818, 2019
SstI GAGCTC 1 cut(s) 1108
StuI AGGCCT 1 cut(s) 1816
StyD4I CCNGG 5 cut(s) 901, 1556, 1741, 1774, 1792
StyI CCWWGG 6 cut(s) 219, 627, 854, 1258, 1614, 1817
TaaI ACNGT 5 cut(s) 422, 488, 1214, 1448, 1844
TaqI TCGA 4 cut(s) 505, 1337, 1911, 2284
TaqII GACCGA 1 cut(s) 1049
TatI WGTACW 5 cut(s) 481, 537, 1332, 1519, 1653
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 4 cut(s) 697, 1583, 1852, 2230
Tru1I TTAA 7 cut(s) 192, 303, 801, 1899, 1998, 2189, 2295
Tru9I TTAA 7 cut(s) 192, 303, 801, 1899, 1998, 2189, 2295
TscAI CASTG 5 cut(s) 714, 1120, 1309, 1854, 1948
TseFI GTSAC 3 cut(s) 821, 868, 2192
TseI GCWGC 6 cut(s) 19, 86, 241, 461, 1064, 2134
Tsp45I GTSAC 3 cut(s) 821, 868, 2192
TspDTI ATGAA 9 cut(s) 471, 648, 951, 996, 1212, 1308, 1985, 2074, 2102
TspGWI ACGGA 3 cut(s) 26, 286, 369
TspRI CASTG 5 cut(s) 714, 1120, 1309, 1854, 1948
Van91I CCANNNNNTGG 1 cut(s) 1157
Vha464I CTTAAG 1 cut(s) 2188
VpaK11BI GGWCC 1 cut(s) 273
VspI ATTAAT 2 cut(s) 192, 1899
XagI CCTNNNNNAGG 1 cut(s) 652
XapI RAATTY 2 cut(s) 1525, 1984
XceI RCATGY 1 cut(s) 2200
XhoI CTCGAG 1 cut(s) 1336
XmaJI CCTAGG 1 cut(s) 1817
XmiI GTMKAC 1 cut(s) 1876
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 6 cut(s) 278, 308, 876, 1094, 1818, 2019
ZrmI AGTACT 3 cut(s) 539, 1334, 1655
Zsp2I ATGCAT 3 cut(s) 550, 1612, 2202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.