Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004041

Physical Location & Seq

Genomic Coordinates Forward (+)

20363002 .. 20368375

Gene Structure 5374 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1128600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

ATGAGTGTTAATAGTGATGAGGACAAACAACTGTTGGAACTGGACCACCGCTCATGGGAGGGTGAGGGGAAGAGGGGTAACAATGGAGTGGAGGATGAGGAAAGGAGGCTAGTGGAGGAAGATGAGTCGAAAAGTAATGGAAGAGCTGGAGATGTAAAAGGTCGGCAAGGAGGGGCTGTTCAAGCCCAGACTGTTACGCAACAGCCTCAAGGGTCAACGGTATGCTGGGAGAGGTTTCTACCACTAAGATCTCTTAAGGTTTTACTGGTGGAATATGATGATTGCACTCGCCATGTTGTCACCGCACTGCTTCGCAATTGCAGTTATGAAGTGATTCCGGTAGCAAATGGAGTGCAAGCATGGAAGATTCTTGAAGATTTGACTAATCATGTTGATCTAGTATTAACTGAGGTAGTCATGCCGTGCGTATCTGGCGTTGGTCTTTTAAGCAAGATTATGAACCATAAGACCCGGAAAAATGTTCCTGTGATTATGATGTCGTCTCATGATTCAATGGGTCTCGTCTTTAAGTGTTTGTCAAAGGGTGCTGTTGACTTTTTGGTGAAGCCTATTCGAAAGAATGAGCTTAAGAACCTCTGGCAGCACATTTGGAGGAGATGCCATAGTTCTAGTGGCAGTGGTACTGGAAGTGAAAGTGGCACACAAACTCAAAGGTCCATAAGATCAAAAAGTGTTGAAAAGTCCGGGAACAATAGCGGAAGCAATGATGAGGAAGATAATGTAAGCACTGATTTGAATATTGGAGATGGAAGTGACAATGGGAGTGGCACTCAGAGTTCGTGGACAAAAAAAGCTGTAGAAGTTGACAGTCCTCGACATGCATATTCATGGCCCCATCCCGATAGTACTTGTTGCCAGCAACAGAAGGAACAAAATGACAACTTTGCAATGGGAAAAGATCTGGAGATATGCCTGACCGGTACTAAAGATTTACAGCACGGGCAACGTAATGATTTTTCAACAAAACTAGCAGGAACAAGACAGAATAATCTGCTTGAGACAGGTTCCTGTAAACGTGACGAGAAAATTGATAAGGGCAAGCTGAATTTGAATCATGAGAGTCCTTCGAGCATGCTAAAGTATGATGGTGCTACTGTAACAGGTGTTGTCACTAATCCCACTGATTCCCAGATGGACAACACACAATTTGAAGCTTCTAACAGACATTATAACGCCTTAGATGTTAACAATAAGGTGAATAGTGATACTCATGAACTACCATCCGTGGAGCTCAGTTTGAAAAGGCTTAGAGGAGTCGAAGGCACCGAGACAACAGTACAAGATGACCGCAATGTCTTGAGACGTTCAGACTATTCAGCCTTTTCAAGGTATAATGCAGCCTCAAATGCTAACAAGGGTCCTTCTGGGCATGTCGGAAGCAACTCTCCACATGATAATAGTGGAGAAGTTACAAAAAAGGAATTCTCTTGTGACATTCGATCTCATTCAAGAGATAACCCTCCCAATCAATGCTCGATTGGAGGCAGAAACATCAACGATATGGGGTCTACTACTAATAACGATGTTCCGAAATCTCAAGTTGCAAACAAGTCAGCAGCAGCAGCAGCATCCACAGTCCAACTTTTGCATCCATCATCTGCTTTCCATCCTGTGAAGGACCTTAAGAGTGCTACCGAACATGTTATAGTAGATAATGCTAATGATGTGGAAACAGCAATACGAATTCAATCAAGAGACACCCAGAAGCAACTGCAAATTCAGAACCTCCATCATGATTATGATCAGTATCGGGTCCATCACCATGTTGTCTACAATAAGCAAGAGCAACATCTTCGTGACAATAATGATTTATCTTTAAATAAATTGGCTGCAGCTGCCCCACATTGCGGATCATCCAATGGGACTGGGCCTGTTGAAGGCAATCCTGGAAATTATAGCATCAATGGGAGTGGTTCAGGCAGCAACCATGGGAGCAATGGGCAAAACGGAGGCAGCGGTGGACATAATGTTGGAGGTACAAACCTAGAAAGTGATTATGGAGTTGCTGGGAAAAGTGGAAGTGGTGATGGTAGCGGAAACCAGAGTGGAAATAGAGTAGATGAAAACAAGAAGCAGAGAGAAGCTGCCTTGACCAAGTTCCGTCAGAAAAGAACAGAGAGATGCTTCCGGAAAAAGGTTCGGTATCAGAACAGAAAGAAACTGGCAGACCAACGGCCTCGCATTCGAGGACAGTTTGTGCGGCATACAGTGAGTGAGAACACGAGCAGGACAACAGATATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006355 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010017 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0048511 GO:0048519 GO:0048523 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0080090 GO:0097159 GO:0104004 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

CCT Response_reg

Protein Analysis

755

Amino Acids

82.81

Weight (kDa)

7.89

Isoelectric Point (pI)

38.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	87 - 198	1.3e-19	Response regulator receiver domain
CCT	PF06203	700 - 741	6e-16	CCT motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004041)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02810 AT5G02810
fragaria_vesca	FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060 FvH4_6g15060
malus_domestica	MD04G1113200.v1.1 MD12G1128600.v1.1
prunus_persica	Prupe.6G244200_v2.0.a1 Prupe.6G244200_v2.0.a1 Prupe.6G244200_v2.0.a1 Prupe.6G244200_v2.0.a1
pyrus_communis	pycom04g10210 pycom12g12520
rosa_chinensis	RchiOBHm_Chr3g0468241
rosa_laevigata	RLG00000024439
rosa_multiflora	Rmu_sc0004524.1_g000003 Rmu_sc0005436.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412880
rosa_rugosa	Rorug03G0094600 Rorug03G0094600 Rorug03G0094700
rosa_samantha	Rh3AG142100 Rh3BG163600 Rh3DG164300
rosa_wichuraiana	Rw3G013450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1191
AasI	GACNNNNNNGTC	1 cut(s)	1313
AccB1I	GGYRCC	1 cut(s)	1283
AccBSI	CCGCTC	1 cut(s)	51
AccI	GTMKAC	2 cut(s)	1529, 1791
AccIII	TCCGGA	1 cut(s)	2148
AciI	CCGC	8 cut(s)	49, 303, 717, 1309, 1869, 1977, 2055, 2221
AclWI	GGATC	1 cut(s)	1879
AcsI	RAATTY	4 cut(s)	1066, 1442, 1704, 1737
AfaI	GTAC	5 cut(s)	643, 868, 943, 1299, 1999
AfiI	CCNNNNNNNGG	5 cut(s)	55, 1347, 1868, 1898, 2155
AflII	CTTAAG	3 cut(s)	254, 587, 1643
AflIII	ACRYGT	1 cut(s)	1660
AgeI	ACCGGT	1 cut(s)	938
AjnI	CCWGG	1 cut(s)	1906
AloI	GAACNNNNNNTCC	2 cut(s)	162, 194
AluBI	AGCT	8 cut(s)	146, 586, 815, 1063, 1175, 1252, 1856, 2105
AluI	AGCT	8 cut(s)	146, 586, 815, 1063, 1175, 1252, 1856, 2105
Alw21I	GWGCWC	1 cut(s)	1254
Alw26I	GTCTC	6 cut(s)	507, 524, 1013, 1283, 1315, 1710
AlwI	GGATC	1 cut(s)	1879
Aor13HI	TCCGGA	1 cut(s)	2148
AoxI	GGCC	3 cut(s)	851, 1889, 2195
ApoI	RAATTY	4 cut(s)	1066, 1442, 1704, 1737
AsiGI	ACCGGT	1 cut(s)	938
Asp700I	GAANNNNTTC	1 cut(s)	333
AspS9I	GGNCC	7 cut(s)	43, 675, 852, 1379, 1639, 1774, 1889
AsuC2I	CCSGG	2 cut(s)	472, 706
AsuHPI	GGTGA	6 cut(s)	74, 292, 574, 1228, 1772, 2057
AsuII	TTCGAA	1 cut(s)	574
AvaII	GGWCC	5 cut(s)	43, 675, 1379, 1639, 1774
BanI	GGYRCC	1 cut(s)	1283
BanII	GRGCYC	1 cut(s)	1254
BauI	CACGAG	1 cut(s)	2242
Bbv12I	GWGCWC	1 cut(s)	1254
BceAI	ACGGC	2 cut(s)	406, 2210
BcgI	CGANNNNNNTGC	2 cut(s)	1796, 1830
BciT130I	CCWGG	1 cut(s)	1908
BclI	TGATCA	1 cut(s)	1762
BcnI	CCSGG	2 cut(s)	472, 706
BcoDI	GTCTC	6 cut(s)	507, 524, 1013, 1283, 1315, 1710
BfaI	CTAG	5 cut(s)	110, 398, 630, 989, 2006
BfmI	CTRYAG	2 cut(s)	816, 1851
BfrI	CTTAAG	3 cut(s)	254, 587, 1643
BglII	AGATCT	2 cut(s)	248, 919
BmcAI	AGTACT	1 cut(s)	868
Bme1390I	CCNGG	3 cut(s)	472, 706, 1908
Bme18I	GGWCC	5 cut(s)	43, 675, 1379, 1639, 1774
BmgT120I	GGNCC	7 cut(s)	43, 675, 852, 1379, 1639, 1774, 1889
BmiI	GGNNCC	5 cut(s)	854, 1027, 1285, 1380, 1775
BmrFI	CCNGG	3 cut(s)	472, 706, 1908
BmrI	ACTGGG	1 cut(s)	1896
BmsI	GCATC	5 cut(s)	608, 1598, 1618, 1929, 2132
BmuI	ACTGGG	1 cut(s)	1896
BplI	GAGNNNNNCTC	2 cut(s)	775, 807
BpmI	CTGGAG	2 cut(s)	168, 944
Bpu14I	TTCGAA	1 cut(s)	574
BpuEI	CTTGAG	4 cut(s)	192, 1037, 1339, 1542
BpuMI	CCSGG	2 cut(s)	472, 706
BsaBI	GATNNNNATC	2 cut(s)	1761, 1767
BsaI	GGTCTC	1 cut(s)	524
BsaJI	CCNNGG	2 cut(s)	1245, 1948
BsaWI	WCCGGW	3 cut(s)	337, 938, 2148
BsaXI	ACNNNNNCTCC	2 cut(s)	162, 192
Bsc4I	CCNNNNNNNGG	5 cut(s)	55, 1347, 1868, 1898, 2155
Bse118I	RCCGGY	1 cut(s)	938
Bse1I	ACTGG	5 cut(s)	45, 270, 649, 1891, 2187
Bse3DI	GCAATG	5 cut(s)	730, 915, 1318, 1864, 1963
Bse8I	GATNNNNATC	2 cut(s)	1761, 1767
BseAI	TCCGGA	1 cut(s)	2148
BseBI	CCWGG	1 cut(s)	1908
BseDI	CCNNGG	2 cut(s)	1245, 1948
BseGI	GGATG	7 cut(s)	100, 856, 1241, 1589, 1609, 1627, 1874
BseJI	GATNNNNATC	2 cut(s)	1761, 1767
BseLI	CCNNNNNNNGG	5 cut(s)	55, 1347, 1868, 1898, 2155
BseMI	GCAATG	5 cut(s)	730, 915, 1318, 1864, 1963
BseMII	CTCAG	3 cut(s)	399, 806, 1267
BseNI	ACTGG	5 cut(s)	45, 270, 649, 1891, 2187
BseRI	GAGGAG	2 cut(s)	628, 1287
BseYI	CCCAGC	2 cut(s)	225, 2027
BshFI	GGCC	3 cut(s)	853, 1891, 2197
BshNI	GGYRCC	1 cut(s)	1283
BshTI	ACCGGT	1 cut(s)	938
BsiHKAI	GWGCWC	1 cut(s)	1254
BsiSI	CCGG	5 cut(s)	338, 472, 705, 939, 2149
BslFI	GGGAC	1 cut(s)	1897
BslI	CCNNNNNNNGG	5 cut(s)	55, 1347, 1868, 1898, 2155
BsmAI	GTCTC	6 cut(s)	507, 524, 1013, 1283, 1315, 1710
BsmBI	CGTCTC	2 cut(s)	507, 1315
BsmFI	GGGAC	1 cut(s)	1897
BsmI	GAATGC	1 cut(s)	2202
BsnI	GGCC	3 cut(s)	853, 1891, 2197
Bso31I	GGTCTC	1 cut(s)	524
Bsp119I	TTCGAA	1 cut(s)	574
Bsp1286I	GDGCHC	1 cut(s)	1254
Bsp13I	TCCGGA	1 cut(s)	2148
Bsp143I	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
Bsp19I	CCATGG	1 cut(s)	1948
BspACI	CCGC	8 cut(s)	49, 303, 717, 1309, 1869, 1977, 2055, 2221
BspANI	GGCC	3 cut(s)	853, 1891, 2197
BspCNI	CTCAG	3 cut(s)	400, 805, 1266
BspEI	TCCGGA	1 cut(s)	2148
BspHI	TCATGA	4 cut(s)	505, 1075, 1231, 1753
BspLI	GGNNCC	5 cut(s)	854, 1027, 1285, 1380, 1775
BspMAI	CTGCAG	1 cut(s)	1855
BspPI	GGATC	1 cut(s)	1879
BspQI	GCTCTTC	1 cut(s)	136
BspT104I	TTCGAA	1 cut(s)	574
BspT107I	GGYRCC	1 cut(s)	1283
BspTI	CTTAAG	3 cut(s)	254, 587, 1643
BspTNI	GGTCTC	1 cut(s)	524
BsrBI	CCGCTC	1 cut(s)	51
BsrDI	GCAATG	5 cut(s)	730, 915, 1318, 1864, 1963
BsrFI	RCCGGY	1 cut(s)	938
BsrI	ACTGG	5 cut(s)	45, 270, 649, 1891, 2187
BssAI	RCCGGY	1 cut(s)	938
BssECI	CCNNGG	2 cut(s)	1245, 1948
BssMI	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
BssSI	CACGAG	1 cut(s)	2242
BssT1I	CCWWGG	1 cut(s)	1948
Bst2BI	CACGAG	1 cut(s)	2242
Bst2UI	CCWGG	1 cut(s)	1908
Bst4CI	ACNGT	9 cut(s)	33, 193, 220, 830, 1117, 1297, 1597, 2214, 2230
Bst6I	CTCTTC	2 cut(s)	65, 136
BstAFI	CTTAAG	3 cut(s)	254, 587, 1643
BstBI	TTCGAA	1 cut(s)	574
BstC8I	GCNNGC	4 cut(s)	357, 878, 1061, 1094
BstDEI	CTNAG	6 cut(s)	245, 408, 792, 1198, 1253, 1268
BstDSI	CCRYGG	2 cut(s)	1245, 1948
BstENI	CCTNNNNNAGG	2 cut(s)	1345, 1896
BstF5I	GGATG	7 cut(s)	100, 856, 1241, 1589, 1609, 1627, 1874
BstKTI	GATC	7 cut(s)	251, 397, 686, 922, 1463, 1765, 1874
BstMAI	GTCTC	6 cut(s)	507, 524, 1013, 1283, 1315, 1710
BstMBI	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
BstMWI	GCNNNNNNNGC	6 cut(s)	182, 432, 1367, 1583, 1586, 1856
BstNI	CCWGG	1 cut(s)	1908
BstNSI	RCATGY	4 cut(s)	842, 1096, 1394, 1664
BstSCI	CCNGG	3 cut(s)	470, 704, 1906
BstSFI	CTRYAG	2 cut(s)	816, 1851
BstX2I	RGATCY	2 cut(s)	248, 919
BstYI	RGATCY	2 cut(s)	248, 919
BsuRI	GGCC	3 cut(s)	853, 1891, 2197
BtgI	CCRYGG	2 cut(s)	1245, 1948
BtsCI	GGATG	7 cut(s)	100, 856, 1241, 1589, 1609, 1627, 1874
BtsI	GCAGTG	2 cut(s)	305, 643
BtsIMutI	CAGTG	5 cut(s)	305, 643, 747, 1140, 2235
Cac8I	GCNNGC	4 cut(s)	357, 878, 1061, 1094
CciI	TCATGA	4 cut(s)	505, 1075, 1231, 1753
Cfr10I	RCCGGY	1 cut(s)	938
Cfr13I	GGNCC	7 cut(s)	43, 675, 852, 1379, 1639, 1774, 1889
Csp6I	GTAC	5 cut(s)	642, 867, 942, 1298, 1998
CspAI	ACCGGT	1 cut(s)	938
CspCI	CAANNNNNGTGG	4 cut(s)	766, 801, 1912, 1947
CviQI	GTAC	5 cut(s)	642, 867, 942, 1298, 1998
DdeI	CTNAG	6 cut(s)	245, 408, 792, 1198, 1253, 1268
DpnI	GATC	7 cut(s)	250, 396, 685, 921, 1462, 1764, 1873
DpnII	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
DraI	TTTAAA	1 cut(s)	1839
DrdI	GACNNNNNNGTC	1 cut(s)	1313
DseDI	GACNNNNNNGTC	1 cut(s)	1313
Eam1104I	CTCTTC	2 cut(s)	65, 136
EarI	CTCTTC	2 cut(s)	65, 136
Ecl136II	GAGCTC	1 cut(s)	1252
Eco130I	CCWWGG	1 cut(s)	1948
Eco24I	GRGCYC	1 cut(s)	1254
Eco31I	GGTCTC	1 cut(s)	524
Eco32I	GATATC	1 cut(s)	2260
Eco47I	GGWCC	5 cut(s)	43, 675, 1379, 1639, 1774
Eco53kI	GAGCTC	1 cut(s)	1252
EcoICRI	GAGCTC	1 cut(s)	1252
EcoNI	CCTNNNNNAGG	2 cut(s)	1345, 1896
EcoO109I	RGGNCCY	2 cut(s)	1379, 1639
EcoRI	GAATTC	2 cut(s)	1442, 1704
EcoRII	CCWGG	1 cut(s)	1906
EcoRV	GATATC	1 cut(s)	2260
EcoT14I	CCWWGG	1 cut(s)	1948
EcoT22I	ATGCAT	1 cut(s)	844
EcoT38I	GRGCYC	1 cut(s)	1254
ErhI	CCWWGG	1 cut(s)	1948
Esp3I	CGTCTC	2 cut(s)	507, 1315
FaqI	GGGAC	1 cut(s)	1897
FbaI	TGATCA	1 cut(s)	1762
FblI	GTMKAC	2 cut(s)	1529, 1791
FokI	GGATG	7 cut(s)	107, 843, 1228, 1576, 1596, 1614, 1861
FriOI	GRGCYC	1 cut(s)	1254
FspBI	CTAG	5 cut(s)	110, 398, 630, 989, 2006
GsaI	CCCAGC	2 cut(s)	229, 2031
GsuI	CTGGAG	2 cut(s)	168, 944
HaeIII	GGCC	3 cut(s)	853, 1891, 2197
HapII	CCGG	5 cut(s)	338, 472, 705, 939, 2149
HincII	GTYRAC	4 cut(s)	216, 553, 826, 1207
HindII	GTYRAC	4 cut(s)	216, 553, 826, 1207
HindIII	AAGCTT	1 cut(s)	1173
HinfI	GANTC	8 cut(s)	125, 334, 367, 509, 1072, 1081, 1145, 1275
HpaI	GTTAAC	1 cut(s)	1207
HpaII	CCGG	5 cut(s)	338, 472, 705, 939, 2149
HphI	GGTGA	6 cut(s)	74, 292, 574, 1228, 1772, 2057
Hpy166II	GTNNAC	9 cut(s)	216, 553, 804, 826, 1034, 1207, 1530, 1792, 1982
Hpy188I	TCNGA	7 cut(s)	795, 1330, 1397, 1551, 1743, 2127, 2169
Hpy8I	GTNNAC	9 cut(s)	216, 553, 804, 826, 1034, 1207, 1530, 1792, 1982
HpyAV	CCTTC	6 cut(s)	880, 1095, 1274, 1392, 1630, 1892
HpyCH4III	ACNGT	9 cut(s)	33, 193, 220, 830, 1117, 1297, 1597, 2214, 2230
HpyCH4IV	ACGT	3 cut(s)	967, 1036, 1324
HpyF10VI	GCNNNNNNNGC	6 cut(s)	182, 432, 1367, 1583, 1586, 1856
HpyF3I	CTNAG	6 cut(s)	245, 408, 792, 1198, 1253, 1268
HpySE526I	ACGT	3 cut(s)	967, 1036, 1324
Kpn2I	TCCGGA	1 cut(s)	2148
Ksp22I	TGATCA	1 cut(s)	1762
KspAI	GTTAAC	1 cut(s)	1207
Kzo9I	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
LguI	GCTCTTC	1 cut(s)	136
LmnI	GCTCC	2 cut(s)	1249, 1953
LweI	GCATC	5 cut(s)	608, 1598, 1618, 1929, 2132
MaeI	CTAG	5 cut(s)	110, 398, 630, 989, 2006
MaeII	ACGT	3 cut(s)	967, 1036, 1324
MalI	GATC	7 cut(s)	250, 396, 685, 921, 1462, 1764, 1873
MbiI	CCGCTC	1 cut(s)	51
MboI	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
MboII	GAAGA	7 cut(s)	82, 131, 153, 376, 386, 746, 1805
MfeI	CAATTG	1 cut(s)	316
MflI	RGATCY	2 cut(s)	248, 919
MhlI	GDGCHC	1 cut(s)	1254
MluCI	AATT	9 cut(s)	316, 1047, 1066, 1166, 1442, 1704, 1737, 1844, 1912
MlyI	GAGTC	3 cut(s)	134, 1090, 1284
MmeI	TCCRAC	4 cut(s)	15, 1375, 1624, 1972
Mph1103I	ATGCAT	1 cut(s)	844
MroI	TCCGGA	1 cut(s)	2148
MroXI	GAANNNNTTC	1 cut(s)	333
MseI	TTAA	9 cut(s)	9, 255, 404, 446, 528, 588, 1206, 1644, 1838
MslI	CAYNNNNRTG	4 cut(s)	847, 1758, 1782, 1815
MspA1I	CMGCKG	2 cut(s)	1856, 1977
MspCI	CTTAAG	3 cut(s)	254, 587, 1643
MspI	CCGG	5 cut(s)	338, 472, 705, 939, 2149
MspR9I	CCNGG	3 cut(s)	472, 706, 1908
MunI	CAATTG	1 cut(s)	316
Mva1269I	GAATGC	1 cut(s)	2202
MvaI	CCWGG	1 cut(s)	1908
MwoI	GCNNNNNNNGC	6 cut(s)	182, 432, 1367, 1583, 1586, 1856
NciI	CCSGG	2 cut(s)	472, 706
NcoI	CCATGG	1 cut(s)	1948
NdeII	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
NlaIV	GGNNCC	5 cut(s)	854, 1027, 1285, 1380, 1775
NmuCI	GTSAC	6 cut(s)	298, 773, 1037, 1129, 1451, 1817
NsiI	ATGCAT	1 cut(s)	844
NspI	RCATGY	4 cut(s)	842, 1096, 1394, 1664
NspV	TTCGAA	1 cut(s)	574
PaeI	GCATGC	1 cut(s)	1096
PagI	TCATGA	4 cut(s)	505, 1075, 1231, 1753
PciI	ACATGT	1 cut(s)	1660
PciSI	GCTCTTC	1 cut(s)	136
PcsI	WCGNNNNNNNCGW	1 cut(s)	1284
PctI	GAATGC	1 cut(s)	2202
PdmI	GAANNNNTTC	1 cut(s)	333
PfeI	GAWTC	5 cut(s)	334, 367, 509, 1072, 1145
PfoI	TCCNGGA	2 cut(s)	704, 1906
PinAI	ACCGGT	1 cut(s)	938
PleI	GAGTC	3 cut(s)	133, 1089, 1283
PpsI	GAGTC	3 cut(s)	133, 1089, 1283
PpuMI	RGGWCCY	2 cut(s)	1379, 1639
PscI	ACATGT	1 cut(s)	1660
PsiI	TTATAA	1 cut(s)	1191
Psp124BI	GAGCTC	1 cut(s)	1254
Psp5II	RGGWCCY	2 cut(s)	1379, 1639
Psp6I	CCWGG	1 cut(s)	1906
PspFI	CCCAGC	2 cut(s)	225, 2027
PspGI	CCWGG	1 cut(s)	1906
PspN4I	GGNNCC	5 cut(s)	854, 1027, 1285, 1380, 1775
PspPI	GGNCC	7 cut(s)	43, 675, 852, 1379, 1639, 1774, 1889
PspPPI	RGGWCCY	2 cut(s)	1379, 1639
PstI	CTGCAG	1 cut(s)	1855
PsuI	RGATCY	2 cut(s)	248, 919
PvuII	CAGCTG	1 cut(s)	1856
RsaI	GTAC	5 cut(s)	643, 868, 943, 1299, 1999
RsaNI	GTAC	5 cut(s)	642, 867, 942, 1298, 1998
RseI	CAYNNNNRTG	4 cut(s)	847, 1758, 1782, 1815
SacI	GAGCTC	1 cut(s)	1254
SapI	GCTCTTC	1 cut(s)	136
SaqAI	TTAA	9 cut(s)	9, 255, 404, 446, 528, 588, 1206, 1644, 1838
Sau3AI	GATC	7 cut(s)	248, 394, 683, 919, 1460, 1762, 1871
Sau96I	GGNCC	7 cut(s)	43, 675, 852, 1379, 1639, 1774, 1889
ScaI	AGTACT	1 cut(s)	868
SchI	GAGTC	3 cut(s)	134, 1090, 1284
ScrFI	CCNGG	3 cut(s)	472, 706, 1908
SduI	GDGCHC	1 cut(s)	1254
SfaNI	GCATC	5 cut(s)	608, 1598, 1618, 1929, 2132
SfcI	CTRYAG	2 cut(s)	816, 1851
SfuI	TTCGAA	1 cut(s)	574
SinI	GGWCC	5 cut(s)	43, 675, 1379, 1639, 1774
SmiMI	CAYNNNNRTG	4 cut(s)	847, 1758, 1782, 1815
SmlI	CTYRAG	7 cut(s)	207, 254, 587, 1016, 1318, 1557, 1643
SmoI	CTYRAG	7 cut(s)	207, 254, 587, 1016, 1318, 1557, 1643
SphI	GCATGC	1 cut(s)	1096
Sse9I	AATT	9 cut(s)	316, 1047, 1066, 1166, 1442, 1704, 1737, 1844, 1912
SsiI	CCGC	8 cut(s)	49, 303, 717, 1309, 1869, 1977, 2055, 2221
SspI	AATATT	1 cut(s)	760
SspMI	CTAG	5 cut(s)	110, 398, 630, 989, 2006
SstI	GAGCTC	1 cut(s)	1254
StyD4I	CCNGG	3 cut(s)	470, 704, 1906
StyI	CCWWGG	1 cut(s)	1948
TaaI	ACNGT	9 cut(s)	33, 193, 220, 830, 1117, 1297, 1597, 2214, 2230
TaiI	ACGT	3 cut(s)	970, 1039, 1327
TaqI	TCGA	8 cut(s)	128, 574, 835, 1088, 1278, 1459, 1496, 2206
TasI	AATT	9 cut(s)	316, 1047, 1066, 1166, 1442, 1704, 1737, 1844, 1912
TatI	WGTACW	2 cut(s)	866, 1297
TauI	GCSGC	1 cut(s)	2224
TfiI	GAWTC	5 cut(s)	334, 367, 509, 1072, 1145
Tru1I	TTAA	9 cut(s)	9, 255, 404, 446, 528, 588, 1206, 1644, 1838
Tru9I	TTAA	9 cut(s)	9, 255, 404, 446, 528, 588, 1206, 1644, 1838
TscAI	CASTG	5 cut(s)	312, 643, 754, 1147, 2235
TseFI	GTSAC	6 cut(s)	298, 773, 1037, 1129, 1451, 1817
Tsp45I	GTSAC	6 cut(s)	298, 773, 1037, 1129, 1451, 1817
TspDTI	ATGAA	5 cut(s)	342, 473, 837, 1248, 2097
TspGWI	ACGGA	3 cut(s)	1234, 1983, 2111
TspRI	CASTG	5 cut(s)	312, 643, 754, 1147, 2235
Vha464I	CTTAAG	3 cut(s)	254, 587, 1643
VpaK11BI	GGWCC	5 cut(s)	43, 675, 1379, 1639, 1774
XagI	CCTNNNNNAGG	2 cut(s)	1345, 1896
XapI	RAATTY	4 cut(s)	1066, 1442, 1704, 1737
XceI	RCATGY	4 cut(s)	842, 1096, 1394, 1664
XcmI	CCANNNNNNNNNTGG	2 cut(s)	629, 1955
XmiI	GTMKAC	2 cut(s)	1529, 1791
XmnI	GAANNNNTTC	1 cut(s)	333
XspI	CTAG	5 cut(s)	110, 398, 630, 989, 2006
ZrmI	AGTACT	1 cut(s)	868
Zsp2I	ATGCAT	1 cut(s)	844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.