Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011657

Physical Location & Seq

Genomic Coordinates Reverse (-)

22226241 .. 22230073

Gene Structure 3833 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1143100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1176 bp

ATGGCGGGGGAGCCACCGTCGTCGGGTGCCCAAATGAGAGTGAACTCACGGCGGCACTACAACGTAGTCCACCCAGTAGACATTGAGACGCCGCCGCTAGCCTCCCCAGCTCCGGCTGTGTCTCCGCCCGTGTACAGAGAGATCAAGCATTTCAAGAAATGGGTTGTCTGGCTGATTCCTGTCTTTGTTGCTGCCAACGTCGTTATGTTCATCATCACCATGTATGTCAACAACTGCCCCAAGAACTCAGTCTCTTGCATTGCCACGTTCTTGGGGAGGTTCTCCTTCCAGCCTTTTAAGGAGAACCCGCTTCTTGGACCGTCCTCATCGACGTTGCAGAAGATGGGGGCTTTGGATGTAAATAAGGTGGTTGACAGACACCAGGGGTGGCTTCTCATCACTTGCAATTGGTTACATGGGGGTGTTTTCCATTTATTGGCTAATATGTTGAGCCTTTTAGTCATCGGATATCGGTTAGAGCAAGAGTTCGGCTTTGTCCGGATTGGGTTGCTATATGTCATCTCTGGACTTGGTGGGAGTTTGTTGTCTTCACTTTTCATCCAAACAAACATCTCTGTTGGTGCTTCTGGTGCACTTTTCGGGTTGCTGGGAGCCATGCTTTCTGAACTTATCACTAATTGGACAATTTATGCTAGTAAGTTTGGAGCACTTTTCACCCTCCTGATCATCATAGCAATCAATTTGGCAGTGGGAACACTCCCACACGTTGACAACTTTGCTCATATTGGAGGATTCCTTTCGGGATTTTTTCTTGGGTTTGTGTTTCTTATCCGCCCCCAGTTTGGATGGGTTAACCAAAGATATACTTCTCCAGGTCCAGGATATGCCTCATCTCAAACAAAATCAAAGTTCAAGACCTATCAGTGCGTTTTGTGGGTCCTCTCTGTGATCGTTTTAATTGTTGGGTTTACAGTTGGCCTCGTTATGCTTCTTCGGGGAGTTGATGCCAATAAGCATTGTTCTTGGTGTCATTATTTATCTTGTGTCCCTACTTCAAGATGGAGCTGCAACTCGGAGCCTGCATACTGCACGACAAACCAGATGGGTGATCAAATCTATTTAACTTGCTCGAGCAACGGTAAGAACGGCACGTATACGGTTCCAAATGCAAGCAGTTCTCAGATCCAGGGGTTATGCTCTCAGCTTTGCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0002791 GO:0002792 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005886 GO:0005911 GO:0006928 GO:0008150 GO:0008283 GO:0009506 GO:0009894 GO:0009966 GO:0009987 GO:0010646 GO:0012505 GO:0016020 GO:0016477 GO:0019222 GO:0023051 GO:0030054 GO:0030162 GO:0031090 GO:0031323 GO:0031329 GO:0031984 GO:0032268 GO:0032879 GO:0032880 GO:0040011 GO:0042058 GO:0042175 GO:0042176 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048870 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051171 GO:0051179 GO:0051223 GO:0051224 GO:0051246 GO:0051674 GO:0055044 GO:0060255 GO:0061136 GO:0065007 GO:0070201 GO:0071944 GO:0080090 GO:0090087 GO:0098588 GO:0098791 GO:0098827 GO:1901184 GO:1903050 GO:1903362 GO:1903530 GO:1903531 GO:1904950

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhomboid

Protein Analysis

392

Amino Acids

42.82

Weight (kDa)

9.13

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhomboid	PF01694	127 - 263	6e-40	Rhomboid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011657)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53780 AT3G53780 AT3G53780
fragaria_vesca	FvH4_6g13560
malus_domestica	MD04G1130000.v1.1 MD12G1143100.v1.1
prunus_persica	Prupe.6G256700_v2.0.a1
pyrus_communis	pycom04g11830 pycom12g13780
rosa_chinensis	RchiOBHm_Chr3g0466421
rosa_laevigata	RLG00000024575
rosa_multiflora	Rmu_co8379997.1_g000002 Rmu_sc0003332.1_g000027 Rmu_sc0003480.1_g000001
rosa_roxburghii	Rroxscaffold_6G00414480
rosa_samantha	Rh3AG128300 Rh3BG149600 Rh3DG150300
rosa_wichuraiana	Rw3G012120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	26
AccB7I	CCANNNNNTGG	1 cut(s)	436
AccI	GTMKAC	2 cut(s)	78, 1115
AccIII	TCCGGA	1 cut(s)	498
AciI	CCGC	7 cut(s)	5, 52, 92, 95, 125, 308, 793
AclWI	GGATC	1 cut(s)	1138
AcyI	GRCGYC	1 cut(s)	89
AfaI	GTAC	1 cut(s)	134
AfiI	CCNNNNNNNGG	6 cut(s)	23, 112, 314, 436, 803, 839
AflIII	ACRYGT	1 cut(s)	724
AgsI	TTSAA	3 cut(s)	154, 874, 1017
AjnI	CCWGG	4 cut(s)	381, 832, 838, 1146
AluBI	AGCT	3 cut(s)	110, 1026, 1165
AluI	AGCT	3 cut(s)	110, 1026, 1165
Alw21I	GWGCWC	2 cut(s)	595, 670
Alw26I	GTCTC	3 cut(s)	80, 126, 256
Alw44I	GTGCAC	1 cut(s)	591
AlwI	GGATC	1 cut(s)	1138
Ama87I	CYCGRG	1 cut(s)	1090
Aor13HI	TCCGGA	1 cut(s)	498
AoxI	GGCC	1 cut(s)	937
ApaLI	GTGCAC	1 cut(s)	591
ApeKI	GCWGC	2 cut(s)	191, 1026
AspS9I	GGNCC	3 cut(s)	317, 836, 898
AsuHPI	GGTGA	3 cut(s)	208, 667, 1079
AsuNHI	GCTAGC	1 cut(s)	97
AvaI	CYCGRG	1 cut(s)	1090
AvaII	GGWCC	3 cut(s)	317, 836, 898
BaeGI	GKGCMC	2 cut(s)	31, 595
BanI	GGYRCC	1 cut(s)	26
BbsI	GAAGAC	1 cut(s)	540
Bbv12I	GWGCWC	2 cut(s)	595, 670
BbvI	GCAGC	2 cut(s)	178, 1013
BccI	CCATC	4 cut(s)	337, 801, 1014, 1057
BceAI	ACGGC	2 cut(s)	65, 1123
BciT130I	CCWGG	4 cut(s)	383, 834, 840, 1148
BclI	TGATCA	2 cut(s)	684, 1069
BcoDI	GTCTC	3 cut(s)	80, 126, 256
BfaI	CTAG	2 cut(s)	98, 654
BisI	GCNGC	5 cut(s)	53, 92, 95, 192, 1027
BlsI	GCNGC	5 cut(s)	54, 93, 96, 193, 1028
Bme1390I	CCNGG	4 cut(s)	383, 834, 840, 1148
Bme18I	GGWCC	3 cut(s)	317, 836, 898
BmeT110I	CYCGRG	1 cut(s)	1090
BmgT120I	GGNCC	3 cut(s)	317, 836, 898
BmiI	GGNNCC	6 cut(s)	12, 28, 613, 899, 1038, 1122
BmrFI	CCNGG	4 cut(s)	383, 834, 840, 1148
BmrI	ACTGGG	2 cut(s)	68, 793
BmsI	GCATC	1 cut(s)	955
BmtI	GCTAGC	1 cut(s)	101
BmuI	ACTGGG	2 cut(s)	68, 793
BpiI	GAAGAC	1 cut(s)	540
BpmI	CTGGAG	1 cut(s)	816
BsaAI	YACGTR	1 cut(s)	1113
BsaHI	GRCGYC	1 cut(s)	89
BsaJI	CCNNGG	2 cut(s)	382, 1147
BsaWI	WCCGGW	1 cut(s)	498
BsaXI	ACNNNNNCTCC	1 cut(s)	32
Bsc4I	CCNNNNNNNGG	6 cut(s)	23, 112, 314, 436, 803, 839
Bse1I	ACTGG	2 cut(s)	74, 799
Bse3DI	GCAATG	1 cut(s)	258
BseAI	TCCGGA	1 cut(s)	498
BseBI	CCWGG	4 cut(s)	383, 834, 840, 1148
BseDI	CCNNGG	2 cut(s)	382, 1147
BseGI	GGATG	3 cut(s)	361, 558, 812
BseLI	CCNNNNNNNGG	6 cut(s)	23, 112, 314, 436, 803, 839
BseMI	GCAATG	1 cut(s)	258
BseMII	CTCAG	3 cut(s)	261, 1154, 1175
BseNI	ACTGG	2 cut(s)	74, 799
BseSI	GKGCMC	2 cut(s)	31, 595
BseXI	GCAGC	2 cut(s)	178, 1013
BseYI	CCCAGC	2 cut(s)	106, 607
BsgI	GTGCAG	1 cut(s)	1033
BshFI	GGCC	1 cut(s)	939
BshNI	GGYRCC	1 cut(s)	26
BsiHKAI	GWGCWC	2 cut(s)	595, 670
BsiHKCI	CYCGRG	1 cut(s)	1090
BsiSI	CCGG	2 cut(s)	113, 499
BslFI	GGGAC	1 cut(s)	992
BslI	CCNNNNNNNGG	6 cut(s)	23, 112, 314, 436, 803, 839
BsmAI	GTCTC	3 cut(s)	80, 126, 256
BsmBI	CGTCTC	1 cut(s)	80
BsmFI	GGGAC	1 cut(s)	992
BsnI	GGCC	1 cut(s)	939
BsoBI	CYCGRG	1 cut(s)	1090
Bsp1286I	GDGCHC	3 cut(s)	31, 595, 670
Bsp13I	TCCGGA	1 cut(s)	498
Bsp1407I	TGTACA	1 cut(s)	132
Bsp143I	GATC	5 cut(s)	141, 684, 909, 1069, 1143
BspACI	CCGC	7 cut(s)	5, 52, 92, 95, 125, 308, 793
BspANI	GGCC	1 cut(s)	939
BspCNI	CTCAG	3 cut(s)	260, 1153, 1174
BspEI	TCCGGA	1 cut(s)	498
BspLI	GGNNCC	6 cut(s)	12, 28, 613, 899, 1038, 1122
BspOI	GCTAGC	1 cut(s)	101
BspPI	GGATC	1 cut(s)	1138
BspT107I	GGYRCC	1 cut(s)	26
BsrDI	GCAATG	1 cut(s)	258
BsrGI	TGTACA	1 cut(s)	132
BsrI	ACTGG	2 cut(s)	74, 799
BssECI	CCNNGG	2 cut(s)	382, 1147
BssMI	GATC	5 cut(s)	141, 684, 909, 1069, 1143
BssNAI	GTATAC	1 cut(s)	1116
BssNI	GRCGYC	1 cut(s)	89
Bst1107I	GTATAC	1 cut(s)	1116
Bst2UI	CCWGG	4 cut(s)	383, 834, 840, 1148
Bst4CI	ACNGT	5 cut(s)	18, 321, 934, 1100, 1120
BstACI	GRCGYC	1 cut(s)	89
BstAUI	TGTACA	1 cut(s)	132
BstBAI	YACGTR	1 cut(s)	1113
BstC8I	GCNNGC	3 cut(s)	99, 1041, 1132
BstDEI	CTNAG	3 cut(s)	247, 1140, 1161
BstF5I	GGATG	3 cut(s)	361, 558, 812
BstKTI	GATC	5 cut(s)	144, 687, 912, 1072, 1146
BstMAI	GTCTC	3 cut(s)	80, 126, 256
BstMBI	GATC	5 cut(s)	141, 684, 909, 1069, 1143
BstMWI	GCNNNNNNNGC	2 cut(s)	107, 590
BstNI	CCWGG	4 cut(s)	383, 834, 840, 1148
BstSCI	CCNGG	4 cut(s)	381, 832, 838, 1146
BstSLI	GKGCMC	2 cut(s)	31, 595
BstV1I	GCAGC	2 cut(s)	178, 1013
BstV2I	GAAGAC	1 cut(s)	540
BstX2I	RGATCY	1 cut(s)	1143
BstXI	CCANNNNNNTGG	1 cut(s)	271
BstYI	RGATCY	1 cut(s)	1143
BstZ17I	GTATAC	1 cut(s)	1116
BsuRI	GGCC	1 cut(s)	939
BtsCI	GGATG	3 cut(s)	361, 558, 812
BtsI	GCAGTG	1 cut(s)	714
BtsIMutI	CAGTG	2 cut(s)	714, 890
Cac8I	GCNNGC	3 cut(s)	99, 1041, 1132
Cfr13I	GGNCC	3 cut(s)	317, 836, 898
CseI	GACGC	1 cut(s)	97
Csp6I	GTAC	1 cut(s)	133
CviAII	CATG	3 cut(s)	220, 416, 616
CviQI	GTAC	1 cut(s)	133
DdeI	CTNAG	3 cut(s)	247, 1140, 1161
DpnI	GATC	5 cut(s)	143, 686, 911, 1071, 1145
DpnII	GATC	5 cut(s)	141, 684, 909, 1069, 1143
EciI	GGCGGA	2 cut(s)	114, 782
Eco32I	GATATC	1 cut(s)	470
Eco47I	GGWCC	3 cut(s)	317, 836, 898
Eco88I	CYCGRG	1 cut(s)	1090
EcoO109I	RGGNCCY	1 cut(s)	898
EcoRII	CCWGG	4 cut(s)	381, 832, 838, 1146
EcoRV	GATATC	1 cut(s)	470
Esp3I	CGTCTC	1 cut(s)	80
FaeI	CATG	3 cut(s)	223, 419, 619
FalI	AAGNNNNNCTT	2 cut(s)	811, 843
FaqI	GGGAC	1 cut(s)	992
FatI	CATG	3 cut(s)	219, 415, 615
FauI	CCCGC	1 cut(s)	315
FbaI	TGATCA	2 cut(s)	684, 1069
FblI	GTMKAC	2 cut(s)	78, 1115
Fnu4HI	GCNGC	5 cut(s)	53, 92, 95, 192, 1027
FokI	GGATG	3 cut(s)	368, 545, 819
Fsp4HI	GCNGC	5 cut(s)	53, 92, 95, 192, 1027
FspBI	CTAG	2 cut(s)	98, 654
GluI	GCNGC	5 cut(s)	53, 92, 95, 192, 1027
GsaI	CCCAGC	2 cut(s)	110, 611
GsuI	CTGGAG	1 cut(s)	816
HaeIII	GGCC	1 cut(s)	939
HapII	CCGG	2 cut(s)	113, 499
HgaI	GACGC	1 cut(s)	97
Hin1I	GRCGYC	1 cut(s)	89
Hin1II	CATG	3 cut(s)	223, 419, 619
HincII	GTYRAC	4 cut(s)	229, 373, 730, 814
HindII	GTYRAC	4 cut(s)	229, 373, 730, 814
HinfI	GANTC	2 cut(s)	175, 753
HpaI	GTTAAC	1 cut(s)	814
HpaII	CCGG	2 cut(s)	113, 499
HphI	GGTGA	3 cut(s)	208, 667, 1079
Hpy188I	TCNGA	4 cut(s)	467, 625, 1036, 1143
Hpy188III	TCNNGA	7 cut(s)	154, 499, 525, 682, 762, 874, 1017
Hpy99I	CGWCG	4 cut(s)	22, 25, 203, 334
HpyAV	CCTTC	1 cut(s)	295
HpyCH4III	ACNGT	5 cut(s)	18, 321, 934, 1100, 1120
HpyCH4IV	ACGT	6 cut(s)	63, 198, 266, 332, 726, 1112
HpyCH4V	TGCA	9 cut(s)	258, 337, 405, 593, 1029, 1043, 1050, 1130, 1170
HpyF10VI	GCNNNNNNNGC	2 cut(s)	107, 590
HpyF3I	CTNAG	3 cut(s)	247, 1140, 1161
HpySE526I	ACGT	6 cut(s)	63, 198, 266, 332, 726, 1112
Hsp92I	GRCGYC	1 cut(s)	89
Hsp92II	CATG	3 cut(s)	223, 419, 619
Kpn2I	TCCGGA	1 cut(s)	498
Ksp22I	TGATCA	2 cut(s)	684, 1069
KspAI	GTTAAC	1 cut(s)	814
Kzo9I	GATC	5 cut(s)	141, 684, 909, 1069, 1143
LmnI	GCTCC	6 cut(s)	10, 115, 611, 665, 1023, 1036
Lsp1109I	GCAGC	2 cut(s)	178, 1013
LweI	GCATC	1 cut(s)	955
MaeI	CTAG	2 cut(s)	98, 654
MaeII	ACGT	6 cut(s)	63, 198, 266, 332, 726, 1112
MaeIII	GTNAC	1 cut(s)	411
MalI	GATC	5 cut(s)	143, 686, 911, 1071, 1145
MboI	GATC	5 cut(s)	141, 684, 909, 1069, 1143
MboII	GAAGA	3 cut(s)	352, 540, 944
MfeI	CAATTG	1 cut(s)	406
MflI	RGATCY	1 cut(s)	1143
MhlI	GDGCHC	3 cut(s)	31, 595, 670
MluCI	AATT	5 cut(s)	406, 637, 645, 700, 918
MnlI	CCTC	8 cut(s)	112, 270, 334, 689, 743, 859, 911, 950
MroI	TCCGGA	1 cut(s)	498
MseI	TTAA	4 cut(s)	297, 813, 917, 1082
MslI	CAYNNNNRTG	2 cut(s)	218, 420
MspI	CCGG	2 cut(s)	113, 499
MspR9I	CCNGG	4 cut(s)	383, 834, 840, 1148
MunI	CAATTG	1 cut(s)	406
MvaI	CCWGG	4 cut(s)	383, 834, 840, 1148
MwoI	GCNNNNNNNGC	2 cut(s)	107, 590
NdeII	GATC	5 cut(s)	141, 684, 909, 1069, 1143
NheI	GCTAGC	1 cut(s)	97
NlaIII	CATG	3 cut(s)	223, 419, 619
NlaIV	GGNNCC	6 cut(s)	12, 28, 613, 899, 1038, 1122
PaeR7I	CTCGAG	1 cut(s)	1090
PfeI	GAWTC	2 cut(s)	175, 753
PflMI	CCANNNNNTGG	1 cut(s)	436
PfoI	TCCNGGA	1 cut(s)	838
PkrI	GCNGC	5 cut(s)	54, 93, 96, 193, 1028
Ppu21I	YACGTR	1 cut(s)	1113
PpuMI	RGGWCCY	1 cut(s)	898
Psp5II	RGGWCCY	1 cut(s)	898
Psp6I	CCWGG	4 cut(s)	381, 832, 838, 1146
PspFI	CCCAGC	2 cut(s)	106, 607
PspGI	CCWGG	4 cut(s)	381, 832, 838, 1146
PspN4I	GGNNCC	6 cut(s)	12, 28, 613, 899, 1038, 1122
PspPI	GGNCC	3 cut(s)	317, 836, 898
PspPPI	RGGWCCY	1 cut(s)	898
PspXI	VCTCGAGB	1 cut(s)	1090
PsuI	RGATCY	1 cut(s)	1143
RsaI	GTAC	1 cut(s)	134
RsaNI	GTAC	1 cut(s)	133
RseI	CAYNNNNRTG	2 cut(s)	218, 420
SaqAI	TTAA	4 cut(s)	297, 813, 917, 1082
SatI	GCNGC	5 cut(s)	53, 92, 95, 192, 1027
Sau3AI	GATC	5 cut(s)	141, 684, 909, 1069, 1143
Sau96I	GGNCC	3 cut(s)	317, 836, 898
ScrFI	CCNGG	4 cut(s)	383, 834, 840, 1148
SduI	GDGCHC	3 cut(s)	31, 595, 670
SfaNI	GCATC	1 cut(s)	955
Sfr274I	CTCGAG	1 cut(s)	1090
SinI	GGWCC	3 cut(s)	317, 836, 898
SlaI	CTCGAG	1 cut(s)	1090
SmiMI	CAYNNNNRTG	2 cut(s)	218, 420
SmlI	CTYRAG	1 cut(s)	1090
SmoI	CTYRAG	1 cut(s)	1090
Sse9I	AATT	5 cut(s)	406, 637, 645, 700, 918
SsiI	CCGC	7 cut(s)	5, 52, 92, 95, 125, 308, 793
SspMI	CTAG	2 cut(s)	98, 654
StyD4I	CCNGG	4 cut(s)	381, 832, 838, 1146
TaaI	ACNGT	5 cut(s)	18, 321, 934, 1100, 1120
TaiI	ACGT	6 cut(s)	66, 201, 269, 335, 729, 1115
TaqI	TCGA	2 cut(s)	329, 1091
TasI	AATT	5 cut(s)	406, 637, 645, 700, 918
TatI	WGTACW	1 cut(s)	132
TauI	GCSGC	3 cut(s)	55, 94, 97
TfiI	GAWTC	2 cut(s)	175, 753
Tru1I	TTAA	4 cut(s)	297, 813, 917, 1082
Tru9I	TTAA	4 cut(s)	297, 813, 917, 1082
TscAI	CASTG	2 cut(s)	714, 890
TseI	GCWGC	2 cut(s)	191, 1026
TspDTI	ATGAA	2 cut(s)	199, 547
TspRI	CASTG	2 cut(s)	714, 890
Van91I	CCANNNNNTGG	1 cut(s)	436
VneI	GTGCAC	1 cut(s)	591
VpaK11BI	GGWCC	3 cut(s)	317, 836, 898
XhoI	CTCGAG	1 cut(s)	1090
XmiI	GTMKAC	2 cut(s)	78, 1115
XspI	CTAG	2 cut(s)	98, 654

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.