Rh3AG128300

Rhomboid-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0011657
Physical Location & Seq
Forward (+)
11057988 .. 11061722
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG128300.1

Sequence Viewer

Length: 963 bp
ATGGCGGCAGATCACTCTCAATCAGGCACCACCCAGTTGCGGCTGAACTCGCGGCGGAGCTACGTGGTCCACCCCGTTGACATTGAGACGCCACCTCTAACTTCCCCGGCTCCCGCCACGCCCGCCGTGTTCCGGGAAATCAAGCATTTCAAGAAATGGGTTGTCTGGTTGATACCTGCTTTTGTTATCATCAATACCATCATGTTTATCATCACTATGTATGTCAACAACTGCCCCAAGAACTCGGTCTCTTGCATTGCCACGTTCTTGGGGAGGTTCTCGTTCCAGCCTTTTAAGGAGAACCCTCTTCTTGGACCATCATCGTCTACGTTACAGAAGATGGGTGCTTTAGATGTGAAAAAGGTGGTTGATGATCACCAGGGGTGGCTTCTCATCACTTGCAATTGGCTACATGGTGGAGTTTTCCATTTGTTGGCGAATATGCTGAGTCTTTTGGTTATTGGGTACCGGCTAGAGCAAGAATTTGGTTTTGTGCGGATTGGTTTGCTATATGTCATCTCTGGACTTGGTGGGAGTTTGATGTCTTCACTTTTCATCCAGTCAAATATCTCTGTTGGTGCTTCCGGTGCACTTTTTGGATTGCTGGGAGCCATGCTTTCTGAACTTATCACTAACTGGACGATATATGCTAGTAAGTTTGGAGCGCTTTTCACCCTCCTGATCATCATAGCAATCAATTTGGCAGTGGGAATTCTCCCACATGTGGATAACTTTGCTCACATTGGAGGATTCCTTTCGGGTTTCTTTCTGGGGTTCGTATTTCTTATCCGCCCTCAGTTCGGATGGGTTAACCAAAGATATGCTCCTCCAGAGTACAGATCATCTGAAGTTAAATCTAAGTTCAAGACCTATCAGTGCATTTTTTGGATCCTTTCGCTGGTTATTTTAATCGTTGGGTATGCCAATCTGTTTCTATGCTCTTCTAGTACTCTCCATATTTAG

Protein Analysis

320

Amino Acids

35.53

Weight (kDa)

9.32

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 126 - 262 1.2e-40 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011657)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53780 AT3G53780 AT3G53780
fragaria_vesca FvH4_6g13560
malus_domestica MD04G1130000.v1.1 MD12G1143100.v1.1
prunus_persica Prupe.6G256700_v2.0.a1
pyrus_communis pycom04g11830 pycom12g13780
rosa_chinensis RchiOBHm_Chr3g0466421
rosa_laevigata RLG00000024575
rosa_multiflora Rmu_co8379997.1_g000002 Rmu_sc0003332.1_g000027 Rmu_sc0003480.1_g000001
rosa_roxburghii Rroxscaffold_6G00414480
rosa_samantha Rh3AG128300 Rh3BG149600 Rh3DG150300
rosa_wichuraiana Rw3G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 184
Acc65I GGTACC 1 cut(s) 465
AccB1I GGYRCC 2 cut(s) 26, 465
AccB7I CCANNNNNTGG 1 cut(s) 433
AccI GTMKAC 1 cut(s) 326
AccII CGCG 1 cut(s) 52
AciI CCGC 8 cut(s) 5, 40, 52, 55, 114, 123, 496, 790
AclWI GGATC 2 cut(s) 883, 896
AcsI RAATTY 2 cut(s) 482, 711
AcuI CTGAAG 1 cut(s) 867
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 3 cut(s) 467, 836, 949
AfeI AGCGCT 1 cut(s) 666
AfiI CCNNNNNNNGG 7 cut(s) 39, 132, 311, 433, 724, 800, 898
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 2 cut(s) 151, 865
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 1 cut(s) 60
AluI AGCT 1 cut(s) 60
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 2 cut(s) 80, 253
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 2 cut(s) 883, 896
Aor51HI AGCGCT 1 cut(s) 666
ApaLI GTGCAC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 482, 711
Asp718I GGTACC 1 cut(s) 465
AspLEI GCGC 1 cut(s) 667
AspS9I GGNCC 2 cut(s) 67, 314
AsuC2I CCSGG 2 cut(s) 107, 134
AsuHPI GGTGA 2 cut(s) 368, 664
AvaII GGWCC 2 cut(s) 67, 314
BaeGI GKGCMC 1 cut(s) 592
BamHI GGATCC 1 cut(s) 888
BanI GGYRCC 2 cut(s) 26, 465
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 592
BccI CCATC 4 cut(s) 206, 325, 334, 798
BceAI ACGGC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 380
BclI TGATCA 2 cut(s) 373, 681
BcnI CCSGG 2 cut(s) 107, 134
BcoDI GTCTC 2 cut(s) 80, 253
BfaI CTAG 3 cut(s) 473, 651, 945
BfoI RGCGCY 1 cut(s) 668
BfuAI ACCTGC 1 cut(s) 184
BisI GCNGC 3 cut(s) 6, 41, 53
BlsI GCNGC 3 cut(s) 7, 42, 54
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 3 cut(s) 107, 134, 380
Bme18I GGWCC 2 cut(s) 67, 314
BmgT120I GGNCC 2 cut(s) 67, 314
BmiI GGNNCC 5 cut(s) 28, 111, 467, 610, 890
BmrFI CCNGG 3 cut(s) 107, 134, 380
BmrI ACTGGG 1 cut(s) 28
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 537
BpmI CTGGAG 1 cut(s) 813
BpuMI CCSGG 2 cut(s) 107, 134
BsaAI YACGTR 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 2 cut(s) 105, 379
BsaWI WCCGGW 1 cut(s) 584
Bsc4I CCNNNNNNNGG 7 cut(s) 39, 132, 311, 433, 724, 800, 898
Bse118I RCCGGY 1 cut(s) 468
Bse1I ACTGG 3 cut(s) 34, 559, 641
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 2 cut(s) 105, 379
BseGI GGATG 2 cut(s) 555, 809
BseLI CCNNNNNNNGG 7 cut(s) 39, 132, 311, 433, 724, 800, 898
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 437, 809
BseNI ACTGG 3 cut(s) 34, 559, 641
BseRI GAGGAG 1 cut(s) 816
BseSI GKGCMC 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 52
BshNI GGYRCC 2 cut(s) 26, 465
BsiHKAI GWGCWC 1 cut(s) 592
BsiSI CCGG 4 cut(s) 107, 133, 469, 585
BslI CCNNNNNNNGG 7 cut(s) 39, 132, 311, 433, 724, 800, 898
BsmAI GTCTC 2 cut(s) 80, 253
BsmBI CGTCTC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 5 cut(s) 10, 373, 681, 839, 888
BspACI CCGC 8 cut(s) 5, 40, 52, 55, 114, 123, 496, 790
BspCNI CTCAG 2 cut(s) 438, 808
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 5 cut(s) 28, 111, 467, 610, 890
BspMI ACCTGC 1 cut(s) 184
BspPI GGATC 2 cut(s) 883, 896
BspQI GCTCTTC 1 cut(s) 946
BspT107I GGYRCC 2 cut(s) 26, 465
BspTNI GGTCTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 468
BsrI ACTGG 3 cut(s) 34, 559, 641
BssAI RCCGGY 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 105, 379
BssMI GATC 5 cut(s) 10, 373, 681, 839, 888
BssNI GRCGYC 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 380
Bst6I CTCTTC 2 cut(s) 312, 946
BstACI GRCGYC 1 cut(s) 89
BstBAI YACGTR 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 3 cut(s) 446, 795, 858
BstF5I GGATG 2 cut(s) 555, 809
BstFNI CGCG 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 668
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 5 cut(s) 13, 376, 684, 842, 891
BstMAI GTCTC 2 cut(s) 80, 253
BstMBI GATC 5 cut(s) 10, 373, 681, 839, 888
BstMWI GCNNNNNNNGC 3 cut(s) 49, 122, 587
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 1 cut(s) 725
BstSCI CCNGG 3 cut(s) 105, 132, 378
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 1 cut(s) 52
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 888
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 1 cut(s) 888
BtsCI GGATG 2 cut(s) 555, 809
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 711, 881
BveI ACCTGC 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 123
CfoI GCGC 1 cut(s) 667
Cfr10I RCCGGY 1 cut(s) 468
Cfr13I GGNCC 2 cut(s) 67, 314
CseI GACGC 1 cut(s) 97
Csp6I GTAC 3 cut(s) 466, 835, 948
CviAII CATG 4 cut(s) 202, 413, 613, 722
CviJI RGCY 8 cut(s) 43, 60, 110, 289, 388, 409, 472, 611
CviKI_1 RGCY 8 cut(s) 43, 60, 110, 289, 388, 409, 472, 611
CviQI GTAC 3 cut(s) 466, 835, 948
DdeI CTNAG 3 cut(s) 446, 795, 858
DpnI GATC 5 cut(s) 12, 375, 683, 841, 890
DpnII GATC 5 cut(s) 10, 373, 681, 839, 888
Eam1104I CTCTTC 2 cut(s) 312, 946
EarI CTCTTC 2 cut(s) 312, 946
EciI GGCGGA 2 cut(s) 70, 779
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 67, 314
Eco47III AGCGCT 1 cut(s) 666
Eco57I CTGAAG 1 cut(s) 867
EcoRI GAATTC 1 cut(s) 711
EcoRII CCWGG 1 cut(s) 378
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 4 cut(s) 205, 416, 616, 725
FatI CATG 4 cut(s) 201, 412, 612, 721
FauI CCCGC 2 cut(s) 121, 130
FbaI TGATCA 2 cut(s) 373, 681
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 6, 41, 53
FokI GGATG 2 cut(s) 542, 816
Fsp4HI GCNGC 3 cut(s) 6, 41, 53
FspBI CTAG 3 cut(s) 473, 651, 945
GlaI GCGC 1 cut(s) 666
GluI GCNGC 3 cut(s) 6, 41, 53
GsaI CCCAGC 1 cut(s) 608
GsuI CTGGAG 1 cut(s) 813
HaeII RGCGCY 1 cut(s) 668
HapII CCGG 4 cut(s) 107, 133, 469, 585
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 667
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 4 cut(s) 205, 416, 616, 725
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HincII GTYRAC 3 cut(s) 79, 226, 811
HindII GTYRAC 3 cut(s) 79, 226, 811
HinfI GANTC 2 cut(s) 448, 750
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 4 cut(s) 107, 133, 469, 585
HphI GGTGA 2 cut(s) 368, 664
Hpy166II GTNNAC 6 cut(s) 70, 79, 226, 327, 590, 811
Hpy188I TCNGA 3 cut(s) 622, 803, 847
Hpy188III TCNNGA 5 cut(s) 151, 522, 679, 830, 865
Hpy8I GTNNAC 6 cut(s) 70, 79, 226, 327, 590, 811
HpyCH4IV ACGT 3 cut(s) 63, 263, 329
HpyCH4V TGCA 4 cut(s) 255, 402, 590, 879
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 122, 587
HpyF3I CTNAG 3 cut(s) 446, 795, 858
HpySE526I ACGT 3 cut(s) 63, 263, 329
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 4 cut(s) 205, 416, 616, 725
HspAI GCGC 1 cut(s) 665
KpnI GGTACC 1 cut(s) 469
Ksp22I TGATCA 2 cut(s) 373, 681
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 5 cut(s) 10, 373, 681, 839, 888
LguI GCTCTTC 1 cut(s) 946
LmnI GCTCC 5 cut(s) 57, 115, 608, 662, 829
MaeI CTAG 3 cut(s) 473, 651, 945
MaeII ACGT 3 cut(s) 63, 263, 329
MaeIII GTNAC 1 cut(s) 330
MalI GATC 5 cut(s) 12, 375, 683, 841, 890
MboI GATC 5 cut(s) 10, 373, 681, 839, 888
MboII GAAGA 4 cut(s) 299, 349, 537, 933
MfeI CAATTG 1 cut(s) 403
MflI RGATCY 1 cut(s) 888
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 4 cut(s) 403, 482, 697, 711
MlyI GAGTC 1 cut(s) 457
MnlI CCTC 7 cut(s) 105, 267, 315, 686, 740, 804, 837
MseI TTAA 4 cut(s) 294, 810, 852, 908
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 4 cut(s) 107, 133, 469, 585
MspR9I CCNGG 3 cut(s) 107, 134, 380
MunI CAATTG 1 cut(s) 403
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 3 cut(s) 49, 122, 587
NciI CCSGG 2 cut(s) 107, 134
NdeII GATC 5 cut(s) 10, 373, 681, 839, 888
NlaIII CATG 4 cut(s) 205, 416, 616, 725
NlaIV GGNNCC 5 cut(s) 28, 111, 467, 610, 890
NspI RCATGY 1 cut(s) 725
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 946
PfeI GAWTC 1 cut(s) 750
PflMI CCANNNNNTGG 1 cut(s) 433
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 3 cut(s) 7, 42, 54
PleI GAGTC 1 cut(s) 456
PpsI GAGTC 1 cut(s) 456
Ppu21I YACGTR 1 cut(s) 64
PscI ACATGT 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 378
PspFI CCCAGC 1 cut(s) 604
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 5 cut(s) 28, 111, 467, 610, 890
PspPI GGNCC 2 cut(s) 67, 314
PsuI RGATCY 1 cut(s) 888
RsaI GTAC 3 cut(s) 467, 836, 949
RsaNI GTAC 3 cut(s) 466, 835, 948
RseI CAYNNNNRTG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 946
SaqAI TTAA 4 cut(s) 294, 810, 852, 908
SatI GCNGC 3 cut(s) 6, 41, 53
Sau3AI GATC 5 cut(s) 10, 373, 681, 839, 888
Sau96I GGNCC 2 cut(s) 67, 314
ScaI AGTACT 1 cut(s) 949
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 3 cut(s) 107, 134, 380
SduI GDGCHC 1 cut(s) 592
SetI ASST 9 cut(s) 62, 66, 97, 178, 266, 278, 332, 366, 872
SinI GGWCC 2 cut(s) 67, 314
SmiMI CAYNNNNRTG 1 cut(s) 215
Sse9I AATT 4 cut(s) 403, 482, 697, 711
SsiI CCGC 8 cut(s) 5, 40, 52, 55, 114, 123, 496, 790
SspMI CTAG 3 cut(s) 473, 651, 945
StyD4I CCNGG 3 cut(s) 105, 132, 378
TaiI ACGT 3 cut(s) 66, 266, 332
TaqII GACCGA 1 cut(s) 235
TasI AATT 4 cut(s) 403, 482, 697, 711
TatI WGTACW 2 cut(s) 834, 947
TauI GCSGC 3 cut(s) 8, 43, 55
TfiI GAWTC 1 cut(s) 750
Tru1I TTAA 4 cut(s) 294, 810, 852, 908
Tru9I TTAA 4 cut(s) 294, 810, 852, 908
TscAI CASTG 2 cut(s) 711, 881
TspDTI ATGAA 1 cut(s) 544
TspRI CASTG 2 cut(s) 711, 881
Van91I CCANNNNNTGG 1 cut(s) 433
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 2 cut(s) 67, 314
XapI RAATTY 2 cut(s) 482, 711
XceI RCATGY 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 326
XspI CTAG 3 cut(s) 473, 651, 945
ZrmI AGTACT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.