MD12G1171900.v1.1

Chlorophyll synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010287
Physical Location & Seq
Forward (+)
25235456 .. 25239820
4365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1171900.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGCGTCCGTGCTCAATACGGTGTCGTCTATCACCTTATCGAAGCTCAACACCAACCGAGTTCGGGCTCAGTCCGCCTTCGCGCCGGTTTCCGTGTCGTTTACCAGAAGGAGGCTTACTGTTAGGGCTGCGGAGACCGATACCAACGAAGCGGTTGAATCTGCGGTACCGGATAAGGCGCCTGCGAAAGGCGGTTCGAGCTTCAATCAGCTTCTGGGGATTAAAGGAGCTTCAAAGGAAACTAACACATGGAAGATTCGGCTTCAGCTGACAAAACCTGTAACTTGGCCTCCACTGGTATGGGGAGTGGTTTGCGGAGCTGCTGCTTCTGGAAATTTTCATTGGACTTTTGAGGACGTTGCTAAATCAATAATGTGCATGTGCATGTCCGGCCCTTTTCTCACTGGTTATACCCAGACTCTGAATGACTGGTATGACCGAGAGATTGATGCAATTAATGAACCTTATCGTCCAATTCCCTCAGGAGCAATATCTGAGAATGAGGTTATTGCTCAAATATGGGTGCTGCTTTTAGGAGGCCTTAGCTTTGCTGGTATATTAGATGTGTGGGCAGGACATAACTTTCCAATATTATTTTACCTTGCTTTGGGTGGATCCTTGCTATCATACATTTACTCTGCTCCACCTTTAAAGCTGAAACAAAATGGATGGATTGGAAATTTTGCCCTTGGTGCAAGTTATATCAGTTTGCCATGGTGGGCTGGTCAGGCATTATTTGGGACCCTCACACCTGACATAATCATCCTCACACTCTTGTACAGCATAGCTGGGTTGGGAATTGCTATTGTAAACGACTTTAAAAGTGTGGAAGGAGATAGAGAACTGGGACTTCAGTCACTCCCTGTAGCTTTTGGTTCTGAAACTGCCAAATGGATTTGTGTTAGTGCCATTGACATCACTCAGTTATCAATCGCTGGTTATCTGCTAGGGGCTGGTAAACCATATCATGCATTAGCTCTCCTTGCCTTGATAGGTCCTCAAGTCTTTTTCCAGTTCAAGTACTTTCTCAAAGACCCCGTCAAGTACGATGTTAAATATCAGGCCAGTGCGCAGCCGTTTCTCGTGCTCGGTCTTTTAGTAACAGCATTGGCGACAAGTCATTGA

Protein Analysis

375

Amino Acids

40.5

Weight (kDa)

8.23

Isoelectric Point (pI)

24.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 97 - 344 2.3e-38 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1071
Acc65I GGTACC 1 cut(s) 166
AccB1I GGYRCC 2 cut(s) 166, 178
AccII CGCG 1 cut(s) 83
AciI CCGC 6 cut(s) 75, 131, 152, 164, 192, 315
AclWI GGATC 2 cut(s) 609, 622
AcsI RAATTY 2 cut(s) 334, 679
AcuI CTGAAG 2 cut(s) 248, 836
AcyI GRCGYC 2 cut(s) 5, 179
AfaI GTAC 4 cut(s) 168, 779, 1022, 1046
AfiI CCNNNNNNNGG 4 cut(s) 64, 111, 188, 607
AgsI TTSAA 4 cut(s) 158, 205, 234, 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 325, 357
Alw21I GWGCWC 2 cut(s) 15, 1089
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 609, 622
AlwNI CAGNNNCTG 2 cut(s) 214, 421
AoxI GGCC 4 cut(s) 287, 391, 538, 1062
ApeKI GCWGC 5 cut(s) 128, 320, 323, 526, 1072
ApoI RAATTY 2 cut(s) 334, 679
ArsI GACNNNNNNTTYG 2 cut(s) 1023, 1055
AseI ATTAAT 1 cut(s) 456
Asp718I GGTACC 1 cut(s) 166
AspLEI GCGC 3 cut(s) 85, 181, 1072
AspS9I GGNCC 3 cut(s) 392, 741, 995
AsuHPI GGTGA 1 cut(s) 25
AvaII GGWCC 2 cut(s) 741, 995
AxyI CCTNAGG 1 cut(s) 481
BamHI GGATCC 1 cut(s) 614
BanI GGYRCC 2 cut(s) 166, 178
BanII GRGCYC 1 cut(s) 70
BarI GAAGNNNNNNTAC 2 cut(s) 100, 132
BauI CACGAG 1 cut(s) 1082
Bbv12I GWGCWC 2 cut(s) 15, 1089
BbvI GCAGC 5 cut(s) 115, 307, 310, 513, 1084
BccI CCATC 1 cut(s) 663
BceAI ACGGC 1 cut(s) 1060
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 947
BfmI CTRYAG 1 cut(s) 864
BfoI RGCGCY 1 cut(s) 182
BisI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
BlsI GCNGC 5 cut(s) 130, 322, 325, 528, 1074
BmcAI AGTACT 1 cut(s) 1022
Bme18I GGWCC 2 cut(s) 741, 995
BmgT120I GGNCC 3 cut(s) 392, 741, 995
BmiI GGNNCC 5 cut(s) 168, 180, 616, 742, 743
BmrI ACTGGG 1 cut(s) 854
BmsI GCATC 1 cut(s) 439
BmuI ACTGGG 1 cut(s) 854
BoxI GACNNNNGTC 1 cut(s) 853
Bpu10I CCTNAGC 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 984
BsaHI GRCGYC 2 cut(s) 5, 179
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 688, 713
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 111, 188, 607
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 6 cut(s) 300, 409, 434, 849, 1012, 1065
Bse21I CCTNAGG 1 cut(s) 481
BseDI CCNNGG 2 cut(s) 688, 713
BseGI GGATG 2 cut(s) 674, 762
BseLI CCNNNNNNNGG 4 cut(s) 64, 111, 188, 607
BseMII CTCAG 4 cut(s) 83, 486, 495, 935
BseNI ACTGG 6 cut(s) 300, 409, 434, 849, 1012, 1065
BseXI GCAGC 5 cut(s) 115, 307, 310, 513, 1084
BseYI CCCAGC 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 4 cut(s) 289, 393, 540, 1064
BshNI GGYRCC 2 cut(s) 166, 178
BsiHKAI GWGCWC 2 cut(s) 15, 1089
BsiSI CCGG 3 cut(s) 86, 170, 390
BslFI GGGAC 2 cut(s) 754, 861
BslI CCNNNNNNNGG 4 cut(s) 64, 111, 188, 607
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 2 cut(s) 754, 861
BsnI GGCC 4 cut(s) 289, 393, 540, 1064
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 3 cut(s) 15, 70, 1089
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 1 cut(s) 614
Bsp19I CCATGG 1 cut(s) 713
BspACI CCGC 6 cut(s) 75, 131, 152, 164, 192, 315
BspANI GGCC 4 cut(s) 289, 393, 540, 1064
BspCNI CTCAG 4 cut(s) 82, 487, 494, 934
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 5 cut(s) 168, 180, 616, 742, 743
BspPI GGATC 2 cut(s) 609, 622
BspT107I GGYRCC 2 cut(s) 166, 178
BspTNI GGTCTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 6 cut(s) 300, 409, 434, 849, 1012, 1065
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 688, 713
BssMI GATC 1 cut(s) 614
BssNI GRCGYC 2 cut(s) 5, 179
BssSI CACGAG 1 cut(s) 1082
BssT1I CCWWGG 2 cut(s) 688, 713
Bst2BI CACGAG 1 cut(s) 1082
Bst4CI ACNGT 2 cut(s) 22, 121
BstACI GRCGYC 2 cut(s) 5, 179
BstAUI TGTACA 1 cut(s) 777
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 5 cut(s) 69, 481, 495, 542, 921
BstDSI CCRYGG 1 cut(s) 713
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 674, 762
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 3 cut(s) 85, 181, 1072
BstKTI GATC 1 cut(s) 617
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 614
BstMWI GCNNNNNNNGC 6 cut(s) 74, 198, 390, 692, 728, 983
BstNSI RCATGY 2 cut(s) 382, 388
BstPAI GACNNNNGTC 1 cut(s) 853
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 115, 307, 310, 513, 1084
BstX2I RGATCY 1 cut(s) 614
BstXI CCANNNNNNTGG 1 cut(s) 300
BstYI RGATCY 1 cut(s) 614
Bsu36I CCTNAGG 1 cut(s) 481
BsuRI GGCC 4 cut(s) 289, 393, 540, 1064
BtgI CCRYGG 1 cut(s) 713
BtsCI GGATG 2 cut(s) 674, 762
BtsIMutI CAGTG 3 cut(s) 293, 402, 1072
Cac8I GCNNGC 1 cut(s) 183
CaiI CAGNNNCTG 2 cut(s) 214, 421
CfoI GCGC 3 cut(s) 85, 181, 1072
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 3 cut(s) 392, 741, 995
Csp6I GTAC 4 cut(s) 167, 778, 1021, 1045
CviAII CATG 5 cut(s) 249, 379, 385, 714, 968
CviQI GTAC 4 cut(s) 167, 778, 1021, 1045
DdeI CTNAG 5 cut(s) 69, 481, 495, 542, 921
DinI GGCGCC 1 cut(s) 180
DpnI GATC 1 cut(s) 616
DpnII GATC 1 cut(s) 614
DraI TTTAAA 2 cut(s) 651, 820
EciI GGCGGA 1 cut(s) 64
Eco130I CCWWGG 2 cut(s) 688, 713
Eco147I AGGCCT 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 741, 995
Eco57I CTGAAG 2 cut(s) 248, 836
Eco81I CCTNAGG 1 cut(s) 481
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoO109I RGGNCCY 2 cut(s) 741, 995
EcoT14I CCWWGG 2 cut(s) 688, 713
EcoT22I ATGCAT 1 cut(s) 973
EcoT38I GRGCYC 1 cut(s) 70
EgeI GGCGCC 1 cut(s) 180
EheI GGCGCC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 688, 713
FaeI CATG 5 cut(s) 252, 382, 388, 717, 971
FaqI GGGAC 2 cut(s) 754, 861
FatI CATG 5 cut(s) 248, 378, 384, 713, 967
Fnu4HI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
FokI GGATG 2 cut(s) 681, 749
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
FspBI CTAG 1 cut(s) 947
FspI TGCGCA 1 cut(s) 1071
GlaI GCGC 3 cut(s) 84, 180, 1071
GluI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
GsaI CCCAGC 1 cut(s) 792
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 4 cut(s) 289, 393, 540, 1064
HapII CCGG 3 cut(s) 86, 170, 390
HhaI GCGC 3 cut(s) 85, 181, 1072
Hin1I GRCGYC 2 cut(s) 5, 179
Hin1II CATG 5 cut(s) 252, 382, 388, 717, 971
Hin6I GCGC 3 cut(s) 83, 179, 1070
HinP1I GCGC 3 cut(s) 83, 179, 1070
HinfI GANTC 3 cut(s) 158, 256, 418
HpaII CCGG 3 cut(s) 86, 170, 390
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 3 cut(s) 102, 811, 959
Hpy188I TCNGA 3 cut(s) 423, 496, 880
Hpy188III TCNNGA 2 cut(s) 330, 483
Hpy8I GTNNAC 3 cut(s) 102, 811, 959
HpyAV CCTTC 3 cut(s) 88, 102, 824
HpyCH4III ACNGT 2 cut(s) 22, 121
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 5 cut(s) 378, 384, 452, 695, 971
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 198, 390, 692, 728, 983
HpyF3I CTNAG 5 cut(s) 69, 481, 495, 542, 921
HpySE526I ACGT 1 cut(s) 357
Hsp92I GRCGYC 2 cut(s) 5, 179
Hsp92II CATG 5 cut(s) 252, 382, 388, 717, 971
HspAI GCGC 3 cut(s) 83, 179, 1070
KasI GGCGCC 1 cut(s) 178
KflI GGGWCCC 1 cut(s) 741
KpnI GGTACC 1 cut(s) 170
Kzo9I GATC 1 cut(s) 614
LmnI GCTCC 4 cut(s) 227, 317, 485, 646
Lsp1109I GCAGC 5 cut(s) 115, 307, 310, 513, 1084
LweI GCATC 1 cut(s) 439
MaeI CTAG 1 cut(s) 947
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 3 cut(s) 280, 855, 1099
MalI GATC 1 cut(s) 616
MboI GATC 1 cut(s) 614
MboII GAAGA 1 cut(s) 265
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 3 cut(s) 15, 70, 1089
MluCI AATT 5 cut(s) 334, 453, 474, 679, 798
Mly113I GGCGCC 1 cut(s) 179
MlyI GAGTC 1 cut(s) 412
MnlI CCTC 9 cut(s) 105, 300, 346, 490, 496, 530, 755, 776, 1008
Mph1103I ATGCAT 1 cut(s) 973
MseI TTAA 5 cut(s) 222, 456, 650, 819, 1053
MslI CAYNNNNRTG 2 cut(s) 298, 383
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 3 cut(s) 86, 170, 390
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 6 cut(s) 74, 198, 390, 692, 728, 983
NarI GGCGCC 1 cut(s) 179
NcoI CCATGG 1 cut(s) 713
NdeII GATC 1 cut(s) 614
NlaIII CATG 5 cut(s) 252, 382, 388, 717, 971
NlaIV GGNNCC 5 cut(s) 168, 180, 616, 742, 743
NmuCI GTSAC 1 cut(s) 855
NsbI TGCGCA 1 cut(s) 1071
NsiI ATGCAT 1 cut(s) 973
NspI RCATGY 2 cut(s) 382, 388
PceI AGGCCT 1 cut(s) 540
PfeI GAWTC 2 cut(s) 158, 256
PflFI GACNNNGTC 1 cut(s) 1037
PkrI GCNGC 5 cut(s) 130, 322, 325, 528, 1074
PleI GAGTC 1 cut(s) 412
PluTI GGCGCC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 412
PpuMI RGGWCCY 2 cut(s) 741, 995
PshAI GACNNNNGTC 1 cut(s) 853
PshBI ATTAAT 1 cut(s) 456
Psp5II RGGWCCY 2 cut(s) 741, 995
PspFI CCCAGC 1 cut(s) 788
PspN4I GGNNCC 5 cut(s) 168, 180, 616, 742, 743
PspPI GGNCC 3 cut(s) 392, 741, 995
PspPPI RGGWCCY 2 cut(s) 741, 995
PstNI CAGNNNCTG 2 cut(s) 214, 421
PsuI RGATCY 1 cut(s) 614
PsyI GACNNNGTC 1 cut(s) 1037
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 4 cut(s) 168, 779, 1022, 1046
RsaNI GTAC 4 cut(s) 167, 778, 1021, 1045
RseI CAYNNNNRTG 2 cut(s) 298, 383
SaqAI TTAA 5 cut(s) 222, 456, 650, 819, 1053
SatI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
Sau3AI GATC 1 cut(s) 614
Sau96I GGNCC 3 cut(s) 392, 741, 995
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 1 cut(s) 412
SduI GDGCHC 3 cut(s) 15, 70, 1089
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 864
SfoI GGCGCC 1 cut(s) 180
SinI GGWCC 2 cut(s) 741, 995
SmiMI CAYNNNNRTG 2 cut(s) 298, 383
SmlI CTYRAG 1 cut(s) 999
SmoI CTYRAG 1 cut(s) 999
Sse9I AATT 5 cut(s) 334, 453, 474, 679, 798
SseBI AGGCCT 1 cut(s) 540
SsiI CCGC 6 cut(s) 75, 131, 152, 164, 192, 315
SspDI GGCGCC 1 cut(s) 178
SspI AATATT 1 cut(s) 591
SspMI CTAG 1 cut(s) 947
StuI AGGCCT 1 cut(s) 540
StyI CCWWGG 2 cut(s) 688, 713
TaaI ACNGT 2 cut(s) 22, 121
TaiI ACGT 1 cut(s) 360
TaqI TCGA 2 cut(s) 41, 197
TaqII GACCGA 3 cut(s) 152, 453, 1079
TasI AATT 5 cut(s) 334, 453, 474, 679, 798
TatI WGTACW 2 cut(s) 777, 1020
TfiI GAWTC 2 cut(s) 158, 256
Tru1I TTAA 5 cut(s) 222, 456, 650, 819, 1053
Tru9I TTAA 5 cut(s) 222, 456, 650, 819, 1053
TscAI CASTG 3 cut(s) 300, 409, 1072
TseFI GTSAC 1 cut(s) 855
TseI GCWGC 5 cut(s) 128, 320, 323, 526, 1072
Tsp45I GTSAC 1 cut(s) 855
TspDTI ATGAA 2 cut(s) 329, 474
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 3 cut(s) 300, 409, 1072
Tth111I GACNNNGTC 1 cut(s) 1037
VpaK11BI GGWCC 2 cut(s) 741, 995
VspI ATTAAT 1 cut(s) 456
XagI CCTNNNNNAGG 1 cut(s) 186
XapI RAATTY 2 cut(s) 334, 679
XceI RCATGY 2 cut(s) 382, 388
XspI CTAG 1 cut(s) 947
ZrmI AGTACT 1 cut(s) 1022
Zsp2I ATGCAT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.