Rh3BG119900

Chlorophyll synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0010287
Physical Location & Seq
Reverse (-)
9883795 .. 9888861
5067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG119900.1

Sequence Viewer

Length: 816 bp
ATGGCGTCCGTACTCAATACGGTGCCTTCCGCTAGATTTTCGAACCCTAACGCCAGCCGAGTTCGTACTCACTCCGTTTTGGCGCCGATTTCCGTTTCGTTTTCCAGGAGGAGATTTACAGTTAGGGCTGCAGAGACTGATACGAATGAAGAAATCAAATCTCAGGCGGCACCGGATAAGGCACCGGCGAGCGGTGGTTCGAGCTTTAATCAGCTTCTTGGAATCAAAGGAGCTTCTCAAGAAACTAATAAATGGAAGATTCGTCTTCAGCTGACCAAGCCTGTTACTTGGCCTCCTTTGGTATGGGGAGTAGTTTGCGGAGCTGCTGCCTCCGGAAATTTTCATTGGAATTTGGAGGATGTTGCCAAATCTATACTTTGTATGTCAATGTCTGGCCCTTTTCTCACTGGCTATACACAGACACTAAATGATTGGTATGACCGAGAGATTGATGCAATCAATGAGCCATATCGTCCAATTCCCTCGGGGGCAATATCTGAGAATGAGGTTATTACTCAAATCTGGGTACTGCTTTTAGGAGGTCTTGTCTCGGCTGGTATATTGGATGTGTGGGCAGGACATGATTTCCCAATAGTCTTTTACCTTGCTCTGGGTGGATCGTTGCTATCGTACATTTACTCTGCTCCACCTCTAAAGCTTAAACAAAATGGATGGATTGGAAATTTTGCGCTTGGAGCAAGTTATATCTCTTTGCCATGGTATCATATCCTATACATAGTATTCTTTAGTAACTACTTAGAATTAACACGATTCATACAGATGTTAGTGGTGATTTGTTTAGAGGTCCTTTTGTAA

Protein Analysis

271

Amino Acids

29.91

Weight (kDa)

7.71

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 98 - 243 9.2e-21 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 22, 82, 169, 181
AccBSI CCGCTC 1 cut(s) 192
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 4 cut(s) 30, 167, 192, 318
AclWI GGATC 1 cut(s) 625
AcsI RAATTY 3 cut(s) 337, 349, 682
AcuI CTGAAG 1 cut(s) 251
AcyI GRCGYC 2 cut(s) 5, 83
AfaI GTAC 4 cut(s) 12, 67, 528, 632
AfiI CCNNNNNNNGG 2 cut(s) 191, 610
AjnI CCWGG 1 cut(s) 104
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
AluBI AGCT 6 cut(s) 204, 214, 233, 271, 323, 658
AluI AGCT 6 cut(s) 204, 214, 233, 271, 323, 658
Alw26I GTCTC 2 cut(s) 128, 553
AlwI GGATC 1 cut(s) 625
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 484
Aor13HI TCCGGA 1 cut(s) 332
AoxI GGCC 2 cut(s) 290, 394
ApeKI GCWGC 3 cut(s) 128, 323, 326
ApoI RAATTY 3 cut(s) 337, 349, 682
AspLEI GCGC 2 cut(s) 85, 691
AspS9I GGNCC 2 cut(s) 395, 805
AsuHPI GGTGA 1 cut(s) 802
AsuII TTCGAA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 484
AvaII GGWCC 1 cut(s) 805
BanI GGYRCC 4 cut(s) 22, 82, 169, 181
BbsI GAAGAC 1 cut(s) 257
BbvI GCAGC 3 cut(s) 115, 310, 313
BccI CCATC 1 cut(s) 666
BciT130I CCWGG 1 cut(s) 106
BcoDI GTCTC 2 cut(s) 128, 553
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 4 cut(s) 129, 168, 324, 327
BlsI GCNGC 4 cut(s) 130, 169, 325, 328
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 805
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 395, 805
BmiI GGNNCC 4 cut(s) 24, 84, 171, 183
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 257
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 222
BsaHI GRCGYC 2 cut(s) 5, 83
BsaJI CCNNGG 2 cut(s) 483, 716
BsaWI WCCGGW 2 cut(s) 172, 332
BsaXI ACNNNNNCTCC 4 cut(s) 277, 307, 531, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 610
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 1 cut(s) 412
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 483, 716
BseGI GGATG 3 cut(s) 364, 571, 677
BseLI CCNNNNNNNGG 2 cut(s) 191, 610
BseMII CTCAG 2 cut(s) 176, 489
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 1 cut(s) 124
BseXI GCAGC 3 cut(s) 115, 310, 313
BshFI GGCC 2 cut(s) 292, 396
BshNI GGYRCC 4 cut(s) 22, 82, 169, 181
BsiHKCI CYCGRG 1 cut(s) 484
BsiSI CCGG 3 cut(s) 173, 185, 333
BslI CCNNNNNNNGG 2 cut(s) 191, 610
BsmAI GTCTC 2 cut(s) 128, 553
BsnI GGCC 2 cut(s) 292, 396
BsoBI CYCGRG 1 cut(s) 484
Bsp119I TTCGAA 1 cut(s) 41
Bsp13I TCCGGA 1 cut(s) 332
Bsp143I GATC 1 cut(s) 617
Bsp19I CCATGG 1 cut(s) 716
BspACI CCGC 4 cut(s) 30, 167, 192, 318
BspANI GGCC 2 cut(s) 292, 396
BspCNI CTCAG 2 cut(s) 175, 490
BspEI TCCGGA 1 cut(s) 332
BspLI GGNNCC 4 cut(s) 24, 84, 171, 183
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 1 cut(s) 625
BspT104I TTCGAA 1 cut(s) 41
BspT107I GGYRCC 4 cut(s) 22, 82, 169, 181
BsrBI CCGCTC 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 1 cut(s) 412
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 483, 716
BssMI GATC 1 cut(s) 617
BssNI GRCGYC 2 cut(s) 5, 83
BssT1I CCWWGG 1 cut(s) 716
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 22, 121
BstACI GRCGYC 2 cut(s) 5, 83
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 55, 190
BstDEI CTNAG 3 cut(s) 162, 498, 757
BstDSI CCRYGG 1 cut(s) 716
BstF5I GGATG 3 cut(s) 364, 571, 677
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 691
BstKTI GATC 1 cut(s) 620
BstMAI GTCTC 2 cut(s) 128, 553
BstMBI GATC 1 cut(s) 617
BstMWI GCNNNNNNNGC 2 cut(s) 277, 695
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 115, 310, 313
BstV2I GAAGAC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 292, 396
BtgI CCRYGG 1 cut(s) 716
BtsCI GGATG 3 cut(s) 364, 571, 677
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 55, 190
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 2 cut(s) 85, 691
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 2 cut(s) 395, 805
Csp6I GTAC 4 cut(s) 11, 66, 527, 631
CviAII CATG 2 cut(s) 581, 717
CviQI GTAC 4 cut(s) 11, 66, 527, 631
DdeI CTNAG 3 cut(s) 162, 498, 757
DinI GGCGCC 1 cut(s) 84
DpnI GATC 1 cut(s) 619
DpnII GATC 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 716
Eco47I GGWCC 1 cut(s) 805
Eco57I CTGAAG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 716
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 2 cut(s) 584, 720
FatI CATG 2 cut(s) 580, 716
Fnu4HI GCNGC 4 cut(s) 129, 168, 324, 327
FokI GGATG 3 cut(s) 371, 578, 684
Fsp4HI GCNGC 4 cut(s) 129, 168, 324, 327
FspBI CTAG 1 cut(s) 33
GlaI GCGC 2 cut(s) 84, 690
GluI GCNGC 4 cut(s) 129, 168, 324, 327
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 2 cut(s) 292, 396
HapII CCGG 3 cut(s) 173, 185, 333
HhaI GCGC 2 cut(s) 85, 691
Hin1I GRCGYC 2 cut(s) 5, 83
Hin1II CATG 2 cut(s) 584, 720
Hin6I GCGC 2 cut(s) 83, 689
HinP1I GCGC 2 cut(s) 83, 689
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 3 cut(s) 222, 259, 771
HpaII CCGG 3 cut(s) 173, 185, 333
HphI GGTGA 1 cut(s) 802
Hpy188I TCNGA 1 cut(s) 499
Hpy188III TCNNGA 2 cut(s) 239, 333
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 2 cut(s) 22, 121
HpyCH4V TGCA 2 cut(s) 131, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 695
HpyF3I CTNAG 3 cut(s) 162, 498, 757
Hsp92I GRCGYC 2 cut(s) 5, 83
Hsp92II CATG 2 cut(s) 584, 720
HspAI GCGC 2 cut(s) 83, 689
KasI GGCGCC 1 cut(s) 82
Kpn2I TCCGGA 1 cut(s) 332
Kzo9I GATC 1 cut(s) 617
LmnI GCTCC 4 cut(s) 230, 320, 649, 695
Lsp1109I GCAGC 3 cut(s) 115, 310, 313
LweI GCATC 1 cut(s) 442
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 283, 749
MalI GATC 1 cut(s) 619
MbiI CCGCTC 1 cut(s) 192
MboI GATC 1 cut(s) 617
MboII GAAGA 3 cut(s) 161, 257, 268
MluCI AATT 5 cut(s) 337, 349, 477, 682, 761
Mly113I GGCGCC 1 cut(s) 83
MnlI CCTC 9 cut(s) 102, 303, 340, 349, 493, 499, 533, 660, 796
MroI TCCGGA 1 cut(s) 332
MseI TTAA 3 cut(s) 207, 660, 764
MslI CAYNNNNRTG 1 cut(s) 779
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 3 cut(s) 173, 185, 333
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 2 cut(s) 277, 695
NarI GGCGCC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 716
NdeII GATC 1 cut(s) 617
NlaIII CATG 2 cut(s) 584, 720
NlaIV GGNNCC 4 cut(s) 24, 84, 171, 183
NmeAIII GCCGAG 2 cut(s) 83, 530
NspV TTCGAA 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 626
PfeI GAWTC 3 cut(s) 222, 259, 771
PfoI TCCNGGA 1 cut(s) 104
PkrI GCNGC 4 cut(s) 130, 169, 325, 328
PluTI GGCGCC 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 805
Psp5II RGGWCCY 1 cut(s) 805
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 4 cut(s) 24, 84, 171, 183
PspPI GGNCC 2 cut(s) 395, 805
PspPPI RGGWCCY 1 cut(s) 805
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 271
RsaI GTAC 4 cut(s) 12, 67, 528, 632
RsaNI GTAC 4 cut(s) 11, 66, 527, 631
RseI CAYNNNNRTG 1 cut(s) 779
SaqAI TTAA 3 cut(s) 207, 660, 764
SatI GCNGC 4 cut(s) 129, 168, 324, 327
Sau3AI GATC 1 cut(s) 617
Sau96I GGNCC 2 cut(s) 395, 805
ScrFI CCNGG 1 cut(s) 106
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 129
SfoI GGCGCC 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 41
SgrAI CRCCGGYG 1 cut(s) 184
SinI GGWCC 1 cut(s) 805
SmiMI CAYNNNNRTG 1 cut(s) 779
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
Sse9I AATT 5 cut(s) 337, 349, 477, 682, 761
SsiI CCGC 4 cut(s) 30, 167, 192, 318
SspDI GGCGCC 1 cut(s) 82
SspMI CTAG 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 2 cut(s) 22, 121
TaqI TCGA 2 cut(s) 41, 200
TaqII GACCGA 1 cut(s) 456
TasI AATT 5 cut(s) 337, 349, 477, 682, 761
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 3 cut(s) 222, 259, 771
Tru1I TTAA 3 cut(s) 207, 660, 764
Tru9I TTAA 3 cut(s) 207, 660, 764
TscAI CASTG 1 cut(s) 412
TseI GCWGC 3 cut(s) 128, 323, 326
TspDTI ATGAA 3 cut(s) 162, 332, 763
TspGWI ACGGA 2 cut(s) 64, 82
TspRI CASTG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 805
XapI RAATTY 3 cut(s) 337, 349, 682
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.