Rh3AG116400

Chlorophyll synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010287
Physical Location & Seq
Reverse (-)
9948874 .. 9955832
6959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG116400.1

Sequence Viewer

Length: 909 bp
ATGGCGTCCGTACTCAATACGGTGCCTTCCGCTAGATTTTCGAACCCTAACGCCAGCCGAGTTCGTACTCACTCCGTTTTGGCGCCGATTTCCGTTTCGTTTTCCAGGAGGAGATTTACAGTTAGGGCTGCAGAGACTGATACGAATGAAGAAATCAAATCTCAGGCGGCACCGGATAAGGCACCGGCGAGCGGTGGTTCGAGCTTTAATCAGCTTCTTGGAATCAAAGGAGCTTCTCAAGAAACTAATAAATGGAAGATTCGTCTTCAGCTGACCAAGCCTGTTACTTGGCCTCCTTTGGTATGGGGAGTAGTTTGCGGAGCTGCTGCCTCCGGAAATTTTCATTGGAATTTGGAGGATGTTGCCAAATCTATACTTTGTATGTCAATGTCTGGCCCTTTTCTCACTGGCTACACACAGACACTAAATGATTGGTATGACCGAGAGATTGATGCAATCAATGAGCCATATCGTCCAATTCCCTCGGGGGCAATATCTGAGAATGAGGTTATTACTCAAATCTGGGTACTGCTTTTAGGAGGTCTTGTCTCGGCTGGTATATTGGATGTGTGGGCAGGACATGATTTCCCAATAGTCTTTTACCTTGCTCTGGGTGGATCCTTGCTATCGTACATTTACTCTGCTCCACCTCTAAAGCTTAAACAAAATGGATGGATTGGAAATTTTGCGCTTGGAGCAAGTTATATCTCTTTGCCATGGTGGGCTGGTCAGGCATTATTTGGAACCCTTACACCTGACATAATTGTCCTCACACTCCTGTACAGCATTGCCGGATTGGGGATTGCTATTGTAAATGACTTCAAAAGTGTTGAAGGAGATAGAGCACTGGGACTTCAGGTCTGTTATATTCTATATTACAGTACTGTAAACAACTTAGATGCCGGATGA

Protein Analysis

302

Amino Acids

32.73

Weight (kDa)

6.3

Isoelectric Point (pI)

29.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 98 - 288 8.8e-30 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 764
AccB1I GGYRCC 4 cut(s) 22, 82, 169, 181
AccBSI CCGCTC 1 cut(s) 192
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 4 cut(s) 30, 167, 192, 318
AclWI GGATC 2 cut(s) 612, 625
AcsI RAATTY 3 cut(s) 337, 349, 682
AcuI CTGAAG 2 cut(s) 251, 839
AcyI GRCGYC 2 cut(s) 5, 83
AfaI GTAC 6 cut(s) 12, 67, 528, 632, 782, 883
AfiI CCNNNNNNNGG 3 cut(s) 191, 610, 798
AgsI TTSAA 2 cut(s) 823, 833
AhdI GACNNNNNGTC 1 cut(s) 857
AjnI CCWGG 1 cut(s) 104
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
AluBI AGCT 6 cut(s) 204, 214, 233, 271, 323, 658
AluI AGCT 6 cut(s) 204, 214, 233, 271, 323, 658
Alw21I GWGCWC 1 cut(s) 847
Alw26I GTCTC 2 cut(s) 128, 553
AlwI GGATC 2 cut(s) 612, 625
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 484
Aor13HI TCCGGA 1 cut(s) 332
AoxI GGCC 2 cut(s) 290, 394
ApeKI GCWGC 3 cut(s) 128, 323, 326
ApoI RAATTY 3 cut(s) 337, 349, 682
AspLEI GCGC 2 cut(s) 85, 691
AspS9I GGNCC 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 484
BamHI GGATCC 1 cut(s) 617
BanI GGYRCC 4 cut(s) 22, 82, 169, 181
BbsI GAAGAC 1 cut(s) 257
Bbv12I GWGCWC 1 cut(s) 847
BbvI GCAGC 3 cut(s) 115, 310, 313
BccI CCATC 1 cut(s) 666
BciT130I CCWGG 1 cut(s) 106
BcoDI GTCTC 2 cut(s) 128, 553
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 4 cut(s) 129, 168, 324, 327
BlsI GCNGC 4 cut(s) 130, 169, 325, 328
BmcAI AGTACT 1 cut(s) 883
Bme1390I CCNGG 1 cut(s) 106
BmeRI GACNNNNNGTC 1 cut(s) 857
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 6 cut(s) 24, 84, 171, 183, 619, 745
BmrFI CCNGG 1 cut(s) 106
BmrI ACTGGG 1 cut(s) 857
BmsI GCATC 2 cut(s) 442, 889
BmuI ACTGGG 1 cut(s) 857
BpiI GAAGAC 1 cut(s) 257
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 222
BsaHI GRCGYC 2 cut(s) 5, 83
BsaJI CCNNGG 2 cut(s) 483, 716
BsaWI WCCGGW 2 cut(s) 172, 332
BsaXI ACNNNNNCTCC 4 cut(s) 277, 307, 531, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 610, 798
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 412, 852
Bse3DI GCAATG 1 cut(s) 786
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 483, 716
BseGI GGATG 3 cut(s) 364, 571, 677
BseLI CCNNNNNNNGG 3 cut(s) 191, 610, 798
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 2 cut(s) 176, 489
BseNI ACTGG 2 cut(s) 412, 852
BseRI GAGGAG 1 cut(s) 124
BseXI GCAGC 3 cut(s) 115, 310, 313
BshFI GGCC 2 cut(s) 292, 396
BshNI GGYRCC 4 cut(s) 22, 82, 169, 181
BsiHKAI GWGCWC 1 cut(s) 847
BsiHKCI CYCGRG 1 cut(s) 484
BsiSI CCGG 5 cut(s) 173, 185, 333, 792, 903
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 3 cut(s) 191, 610, 798
BsmAI GTCTC 2 cut(s) 128, 553
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 2 cut(s) 292, 396
BsoBI CYCGRG 1 cut(s) 484
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 847
Bsp13I TCCGGA 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 1 cut(s) 617
Bsp19I CCATGG 1 cut(s) 716
BspACI CCGC 4 cut(s) 30, 167, 192, 318
BspANI GGCC 2 cut(s) 292, 396
BspCNI CTCAG 2 cut(s) 175, 490
BspEI TCCGGA 1 cut(s) 332
BspLI GGNNCC 6 cut(s) 24, 84, 171, 183, 619, 745
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 2 cut(s) 612, 625
BspT104I TTCGAA 1 cut(s) 41
BspT107I GGYRCC 4 cut(s) 22, 82, 169, 181
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 2 cut(s) 412, 852
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 483, 716
BssMI GATC 1 cut(s) 617
BssNI GRCGYC 2 cut(s) 5, 83
BssT1I CCWWGG 1 cut(s) 716
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 4 cut(s) 22, 121, 881, 886
BstACI GRCGYC 2 cut(s) 5, 83
BstAUI TGTACA 1 cut(s) 780
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 55, 190
BstDEI CTNAG 3 cut(s) 162, 498, 895
BstDSI CCRYGG 1 cut(s) 716
BstF5I GGATG 3 cut(s) 364, 571, 677
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 691
BstKTI GATC 1 cut(s) 620
BstMAI GTCTC 2 cut(s) 128, 553
BstMBI GATC 1 cut(s) 617
BstMWI GCNNNNNNNGC 3 cut(s) 277, 695, 731
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 115, 310, 313
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
BsuRI GGCC 2 cut(s) 292, 396
BtgI CCRYGG 1 cut(s) 716
BtsCI GGATG 3 cut(s) 364, 571, 677
BtsIMutI CAGTG 2 cut(s) 405, 845
Cac8I GCNNGC 2 cut(s) 55, 190
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 2 cut(s) 85, 691
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 1 cut(s) 395
Csp6I GTAC 6 cut(s) 11, 66, 527, 631, 781, 882
CviAII CATG 2 cut(s) 581, 717
CviQI GTAC 6 cut(s) 11, 66, 527, 631, 781, 882
DdeI CTNAG 3 cut(s) 162, 498, 895
DinI GGCGCC 1 cut(s) 84
DpnI GATC 1 cut(s) 619
DpnII GATC 1 cut(s) 617
DrdI GACNNNNNNGTC 1 cut(s) 764
DriI GACNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 764
Eam1105I GACNNNNNGTC 1 cut(s) 857
Eco130I CCWWGG 1 cut(s) 716
Eco57I CTGAAG 2 cut(s) 251, 839
Eco88I CYCGRG 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 716
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 2 cut(s) 584, 720
FaqI GGGAC 1 cut(s) 864
FatI CATG 2 cut(s) 580, 716
Fnu4HI GCNGC 4 cut(s) 129, 168, 324, 327
FokI GGATG 3 cut(s) 371, 578, 684
Fsp4HI GCNGC 4 cut(s) 129, 168, 324, 327
FspBI CTAG 1 cut(s) 33
GlaI GCGC 2 cut(s) 84, 690
GluI GCNGC 4 cut(s) 129, 168, 324, 327
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 2 cut(s) 292, 396
HapII CCGG 5 cut(s) 173, 185, 333, 792, 903
HhaI GCGC 2 cut(s) 85, 691
Hin1I GRCGYC 2 cut(s) 5, 83
Hin1II CATG 2 cut(s) 584, 720
Hin6I GCGC 2 cut(s) 83, 689
HinP1I GCGC 2 cut(s) 83, 689
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 2 cut(s) 222, 259
HpaII CCGG 5 cut(s) 173, 185, 333, 792, 903
Hpy166II GTNNAC 1 cut(s) 889
Hpy188I TCNGA 1 cut(s) 499
Hpy188III TCNNGA 2 cut(s) 239, 333
Hpy8I GTNNAC 1 cut(s) 889
HpyAV CCTTC 2 cut(s) 36, 827
HpyCH4III ACNGT 4 cut(s) 22, 121, 881, 886
HpyCH4V TGCA 2 cut(s) 131, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 277, 695, 731
HpyF3I CTNAG 3 cut(s) 162, 498, 895
Hsp92I GRCGYC 2 cut(s) 5, 83
Hsp92II CATG 2 cut(s) 584, 720
HspAI GCGC 2 cut(s) 83, 689
KasI GGCGCC 1 cut(s) 82
Kpn2I TCCGGA 1 cut(s) 332
Kzo9I GATC 1 cut(s) 617
LmnI GCTCC 4 cut(s) 230, 320, 649, 695
Lsp1109I GCAGC 3 cut(s) 115, 310, 313
LweI GCATC 2 cut(s) 442, 889
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 283
MalI GATC 1 cut(s) 619
MbiI CCGCTC 1 cut(s) 192
MboI GATC 1 cut(s) 617
MboII GAAGA 3 cut(s) 161, 257, 268
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 1 cut(s) 847
MluCI AATT 5 cut(s) 337, 349, 477, 682, 762
Mly113I GGCGCC 1 cut(s) 83
MnlI CCTC 9 cut(s) 102, 303, 340, 349, 493, 499, 533, 660, 779
MroI TCCGGA 1 cut(s) 332
MseI TTAA 2 cut(s) 207, 660
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 5 cut(s) 173, 185, 333, 792, 903
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 3 cut(s) 277, 695, 731
NarI GGCGCC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 716
NdeII GATC 1 cut(s) 617
NlaIII CATG 2 cut(s) 584, 720
NlaIV GGNNCC 6 cut(s) 24, 84, 171, 183, 619, 745
NmeAIII GCCGAG 2 cut(s) 83, 530
NspV TTCGAA 1 cut(s) 41
PfeI GAWTC 2 cut(s) 222, 259
PfoI TCCNGGA 1 cut(s) 104
PkrI GCNGC 4 cut(s) 130, 169, 325, 328
PluTI GGCGCC 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 6 cut(s) 24, 84, 171, 183, 619, 745
PspPI GGNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 1 cut(s) 617
PvuII CAGCTG 1 cut(s) 271
RsaI GTAC 6 cut(s) 12, 67, 528, 632, 782, 883
RsaNI GTAC 6 cut(s) 11, 66, 527, 631, 781, 882
SaqAI TTAA 2 cut(s) 207, 660
SatI GCNGC 4 cut(s) 129, 168, 324, 327
Sau3AI GATC 1 cut(s) 617
Sau96I GGNCC 1 cut(s) 395
ScaI AGTACT 1 cut(s) 883
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 1 cut(s) 847
SfaNI GCATC 2 cut(s) 442, 889
SfcI CTRYAG 1 cut(s) 129
SfoI GGCGCC 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 41
SgrAI CRCCGGYG 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
Sse9I AATT 5 cut(s) 337, 349, 477, 682, 762
SsiI CCGC 4 cut(s) 30, 167, 192, 318
SspDI GGCGCC 1 cut(s) 82
SspMI CTAG 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 4 cut(s) 22, 121, 881, 886
TaqI TCGA 2 cut(s) 41, 200
TaqII GACCGA 1 cut(s) 456
TasI AATT 5 cut(s) 337, 349, 477, 682, 762
TatI WGTACW 2 cut(s) 780, 881
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 222, 259
Tru1I TTAA 2 cut(s) 207, 660
Tru9I TTAA 2 cut(s) 207, 660
TscAI CASTG 2 cut(s) 412, 852
TseI GCWGC 3 cut(s) 128, 323, 326
TspDTI ATGAA 2 cut(s) 162, 332
TspGWI ACGGA 2 cut(s) 64, 82
TspRI CASTG 2 cut(s) 412, 852
XapI RAATTY 3 cut(s) 337, 349, 682
XspI CTAG 1 cut(s) 33
ZrmI AGTACT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.