Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010689

Physical Location & Seq

Genomic Coordinates Forward (+)

26873112 .. 26877238

Gene Structure 4127 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1186500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGAGTTGCAGAGTGAGGAGCGGCGGAAGGGAAGAGAGGGAGCGTGAGCAAGCGGTTTTGAGGAAATCTGTGGAGGGTGTCGAGCCTAAATCAGGCACGCAGAATGGTTTGATTGCTGCCCAGCAGCTGACAATCGACGACAATTTGTTGGTGGACCCGAAATTGTTGTTCATCGGGGCAAAAATTGGTGAGGGAGCGCATGGGAAGGTTTATGAAGGAAGGTACGGAGATCAAATTATGGCTGTCAAAATCCTCCATCGTGGTAGCACTACTGAAGAAAGAGCTGCGCTTGAGGGTCGTTTTGCCCGTGAAGTTAATATGATGTCGCGAGTAAAACATGAGAATCTTGTTAAGTTCATTGGAGCTTGTAAGGAGCCACTAATGGTGATAGTTACTGAGCTATTACCTGGGATGTCACTTAGGAAGTACCTGATGAGTATTCGTCCAAATCCATTAGAGCTCCATGTGGCCATTAAATTTGCTCTTGACATCGCTCATGCTATGGAATGTTTACATGCCAATGGGATCATACATAGAGATCTAAAACCTGATAATTTGTTGCTTACAGCAAATCAGAAACATGTAAAGCTTGCAGATTTTGGTCTTGCAAGAGAAGAAAGTGTTACAGAGATGATGACGGCAGAAACTGGGACTTACCGCTGGATGGCTCCTGAGCTGTATAGCACGGTTACTCTACGCCAGGGAGAGAAGAAGCATTACAATAACAAGGTTGATGTATATAGCTTTGGGATTGTCTTATGGGAATTATTGACTAACCGCATGCCCTTCGAAGGCATGTCCAATTTGCAGGCTGCTTATGCTGCTGCTTTCAAGCATGAGAGGCCTCTACTTCCGCAGGACGTATCCCCTGATCTTGCATTTATCATCCAGTCATGTTGGGTTGAAGACCCTAATCTAAGGCCTACGTTCAGCCAAATCATCCGCATGCTCAACTCGTTACTCTTCAAGCTCTTACCACCCTCACCGCCTATACCAGACACTGACACGAAAGAAGCAGCAGCAAGTAACGGAACAATGACTGAATTCTCTGTCCGGACAAGAGGGAAGTTCGCTTTCCTCAGGCAACTGTTCAATGCCAAGAGGACCAAGAGCTCACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

374

Amino Acids

41.99

Weight (kDa)

9.02

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	59 - 317	2.8e-58	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	60 - 315	3.6e-54	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010689)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01850 AT5G01850
fragaria_vesca	FvH4_6g10040
malus_domestica	MD04G1173100.v1.1 MD12G1186500.v1.1
prunus_persica	Prupe.6G292000_v2.0.a1
pyrus_communis	pycom04g15310
rosa_chinensis	RchiOBHm_Chr3g0460721
rosa_laevigata	RLG00000024985
rosa_multiflora	Rmu_sc0003662.1_g000003 Rmu_sc0011069.1_g000006 Rmu_sc0036518.1_g000001
rosa_roxburghii	Rroxscaffold_6G00419270
rosa_rugosa	Rorug03G0045400 Rorug03G0045500
rosa_samantha	Rh3AG103000 Rh3BG106700 Rh3CG108500 Rh3DG108200
rosa_wichuraiana	Rw3G008770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	21
AccII	CGCG	1 cut(s)	328
AccIII	TCCGGA	1 cut(s)	1053
AciI	CCGC	8 cut(s)	21, 24, 53, 658, 778, 854, 943, 986
AclWI	GGATC	1 cut(s)	533
AcoI	YGGCCR	1 cut(s)	468
AcsI	RAATTY	2 cut(s)	476, 1043
AcuI	CTGAAG	1 cut(s)	294
AfaI	GTAC	2 cut(s)	224, 428
AfiI	CCNNNNNNNGG	3 cut(s)	92, 664, 791
AflIII	ACRYGT	1 cut(s)	580
AgsI	TTSAA	4 cut(s)	832, 905, 967, 1093
AjnI	CCWGG	2 cut(s)	406, 699
Alw21I	GWGCWC	2 cut(s)	462, 1115
AlwI	GGATC	1 cut(s)	533
AlwNI	CAGNNNCTG	3 cut(s)	127, 647, 1001
Aor13HI	TCCGGA	1 cut(s)	1053
AoxI	GGCC	3 cut(s)	468, 842, 920
ApeKI	GCWGC	8 cut(s)	116, 124, 284, 812, 821, 824, 1016, 1019
ApoI	RAATTY	2 cut(s)	476, 1043
AspLEI	GCGC	2 cut(s)	199, 289
AspS9I	GGNCC	2 cut(s)	154, 1104
AsuHPI	GGTGA	3 cut(s)	200, 397, 975
AsuII	TTCGAA	1 cut(s)	789
AvaII	GGWCC	2 cut(s)	154, 1104
AxyI	CCTNAGG	1 cut(s)	1079
BalI	TGGCCA	1 cut(s)	470
BanII	GRGCYC	2 cut(s)	462, 1115
BarI	GAAGNNNNNNTAC	2 cut(s)	701, 733
BbsI	GAAGAC	1 cut(s)	912
Bbv12I	GWGCWC	2 cut(s)	462, 1115
BbvI	GCAGC	8 cut(s)	103, 136, 271, 799, 808, 811, 1028, 1031
BccI	CCATC	2 cut(s)	264, 658
BceAI	ACGGC	1 cut(s)	654
BcgI	CGANNNNNNTGC	2 cut(s)	769, 803
BciT130I	CCWGG	2 cut(s)	408, 701
BciVI	GTATCC	1 cut(s)	874
BfuI	GTATCC	1 cut(s)	874
BglII	AGATCT	1 cut(s)	538
BisI	GCNGC	9 cut(s)	22, 117, 125, 285, 813, 822, 825, 1017, 1020
BlsI	GCNGC	9 cut(s)	23, 118, 126, 286, 814, 823, 826, 1018, 1021
Bme1390I	CCNGG	2 cut(s)	408, 701
Bme18I	GGWCC	2 cut(s)	154, 1104
BmgT120I	GGNCC	2 cut(s)	154, 1104
BmiI	GGNNCC	3 cut(s)	156, 375, 669
BmrFI	CCNGG	2 cut(s)	408, 701
BmrI	ACTGGG	1 cut(s)	657
BmuI	ACTGGG	1 cut(s)	657
BpiI	GAAGAC	1 cut(s)	912
Bpu10I	CCTNAGC	1 cut(s)	672
Bpu14I	TTCGAA	1 cut(s)	789
BpuEI	CTTGAG	1 cut(s)	311
BsaJI	CCNNGG	2 cut(s)	407, 700
BsaWI	WCCGGW	1 cut(s)	1053
Bsc4I	CCNNNNNNNGG	3 cut(s)	92, 664, 791
Bse1I	ACTGG	2 cut(s)	652, 889
Bse21I	CCTNAGG	1 cut(s)	1079
BseAI	TCCGGA	1 cut(s)	1053
BseBI	CCWGG	2 cut(s)	408, 701
BseDI	CCNNGG	2 cut(s)	407, 700
BseGI	GGATG	4 cut(s)	417, 669, 885, 939
BseLI	CCNNNNNNNGG	3 cut(s)	92, 664, 791
BseMII	CTCAG	3 cut(s)	387, 663, 1093
BseNI	ACTGG	2 cut(s)	652, 889
BseRI	GAGGAG	1 cut(s)	31
BseXI	GCAGC	8 cut(s)	103, 136, 271, 799, 808, 811, 1028, 1031
BseYI	CCCAGC	1 cut(s)	120
Bsh1236I	CGCG	1 cut(s)	328
BshFI	GGCC	3 cut(s)	470, 844, 922
BsiHKAI	GWGCWC	2 cut(s)	462, 1115
BsiSI	CCGG	1 cut(s)	1054
BslFI	GGGAC	1 cut(s)	664
BslI	CCNNNNNNNGG	3 cut(s)	92, 664, 791
BsmFI	GGGAC	1 cut(s)	664
BsnI	GGCC	3 cut(s)	470, 844, 922
Bsp119I	TTCGAA	1 cut(s)	789
Bsp1286I	GDGCHC	2 cut(s)	462, 1115
Bsp13I	TCCGGA	1 cut(s)	1053
Bsp143I	GATC	4 cut(s)	229, 525, 538, 871
Bsp68I	TCGCGA	1 cut(s)	328
BspACI	CCGC	8 cut(s)	21, 24, 53, 658, 778, 854, 943, 986
BspANI	GGCC	3 cut(s)	470, 844, 922
BspCNI	CTCAG	3 cut(s)	388, 664, 1092
BspEI	TCCGGA	1 cut(s)	1053
BspFNI	CGCG	1 cut(s)	328
BspLI	GGNNCC	3 cut(s)	156, 375, 669
BspPI	GGATC	1 cut(s)	533
BspT104I	TTCGAA	1 cut(s)	789
BsrBI	CCGCTC	1 cut(s)	21
BsrI	ACTGG	2 cut(s)	652, 889
BssECI	CCNNGG	2 cut(s)	407, 700
BssMI	GATC	4 cut(s)	229, 525, 538, 871
Bst2UI	CCWGG	2 cut(s)	408, 701
Bst4CI	ACNGT	2 cut(s)	688, 1089
Bst6I	CTCTTC	2 cut(s)	27, 968
BstBI	TTCGAA	1 cut(s)	789
BstC8I	GCNNGC	6 cut(s)	51, 98, 591, 782, 810, 947
BstDEI	CTNAG	5 cut(s)	396, 419, 672, 917, 1079
BstENI	CCTNNNNNAGG	1 cut(s)	90
BstF5I	GGATG	4 cut(s)	417, 669, 885, 939
BstFNI	CGCG	1 cut(s)	328
BstHHI	GCGC	2 cut(s)	199, 289
BstKTI	GATC	4 cut(s)	232, 528, 541, 874
BstMBI	GATC	4 cut(s)	229, 525, 538, 871
BstMWI	GCNNNNNNNGC	3 cut(s)	818, 821, 841
BstNI	CCWGG	2 cut(s)	408, 701
BstNSI	RCATGY	5 cut(s)	518, 584, 784, 799, 949
BstSCI	CCNGG	2 cut(s)	406, 699
BstUI	CGCG	1 cut(s)	328
BstV1I	GCAGC	8 cut(s)	103, 136, 271, 799, 808, 811, 1028, 1031
BstV2I	GAAGAC	1 cut(s)	912
BstX2I	RGATCY	1 cut(s)	538
BstYI	RGATCY	1 cut(s)	538
Bsu36I	CCTNAGG	1 cut(s)	1079
BsuI	GTATCC	1 cut(s)	874
BsuRI	GGCC	3 cut(s)	470, 844, 922
BtgZI	GCGATG	1 cut(s)	475
BtsCI	GGATG	4 cut(s)	417, 669, 885, 939
BtsIMutI	CAGTG	1 cut(s)	999
BtuMI	TCGCGA	1 cut(s)	328
Cac8I	GCNNGC	6 cut(s)	51, 98, 591, 782, 810, 947
CaiI	CAGNNNCTG	3 cut(s)	127, 647, 1001
CfoI	GCGC	2 cut(s)	199, 289
Cfr13I	GGNCC	2 cut(s)	154, 1104
Csp6I	GTAC	2 cut(s)	223, 427
CviQI	GTAC	2 cut(s)	223, 427
DdeI	CTNAG	5 cut(s)	396, 419, 672, 917, 1079
DpnI	GATC	4 cut(s)	231, 527, 540, 873
DpnII	GATC	4 cut(s)	229, 525, 538, 871
EaeI	YGGCCR	1 cut(s)	468
Eam1104I	CTCTTC	2 cut(s)	27, 968
EarI	CTCTTC	2 cut(s)	27, 968
EciI	GGCGGA	1 cut(s)	39
Ecl136II	GAGCTC	2 cut(s)	460, 1113
Eco147I	AGGCCT	2 cut(s)	844, 922
Eco24I	GRGCYC	2 cut(s)	462, 1115
Eco47I	GGWCC	2 cut(s)	154, 1104
Eco53kI	GAGCTC	2 cut(s)	460, 1113
Eco57I	CTGAAG	1 cut(s)	294
Eco81I	CCTNAGG	1 cut(s)	1079
EcoICRI	GAGCTC	2 cut(s)	460, 1113
EcoNI	CCTNNNNNAGG	1 cut(s)	90
EcoRI	GAATTC	1 cut(s)	1043
EcoRII	CCWGG	2 cut(s)	406, 699
EcoT38I	GRGCYC	2 cut(s)	462, 1115
FaqI	GGGAC	1 cut(s)	664
Fnu4HI	GCNGC	9 cut(s)	22, 117, 125, 285, 813, 822, 825, 1017, 1020
FokI	GGATG	4 cut(s)	424, 676, 872, 926
FriOI	GRGCYC	2 cut(s)	462, 1115
Fsp4HI	GCNGC	9 cut(s)	22, 117, 125, 285, 813, 822, 825, 1017, 1020
GlaI	GCGC	2 cut(s)	198, 288
GluI	GCNGC	9 cut(s)	22, 117, 125, 285, 813, 822, 825, 1017, 1020
GsaI	CCCAGC	1 cut(s)	124
HaeIII	GGCC	3 cut(s)	470, 844, 922
HapII	CCGG	1 cut(s)	1054
HhaI	GCGC	2 cut(s)	199, 289
Hin6I	GCGC	2 cut(s)	197, 287
HinP1I	GCGC	2 cut(s)	197, 287
HindIII	AAGCTT	1 cut(s)	587
HinfI	GANTC	1 cut(s)	343
HpaII	CCGG	1 cut(s)	1054
HphI	GGTGA	3 cut(s)	200, 397, 975
Hpy166II	GTNNAC	2 cut(s)	154, 512
Hpy188I	TCNGA	1 cut(s)	576
Hpy188III	TCNNGA	4 cut(s)	327, 485, 671, 1054
Hpy8I	GTNNAC	2 cut(s)	154, 512
Hpy99I	CGWCG	1 cut(s)	140
HpyAV	CCTTC	6 cut(s)	21, 199, 209, 213, 785, 796
HpyCH4III	ACNGT	2 cut(s)	688, 1089
HpyCH4IV	ACGT	2 cut(s)	861, 926
HpyCH4V	TGCA	5 cut(s)	9, 593, 608, 808, 878
HpyF10VI	GCNNNNNNNGC	3 cut(s)	818, 821, 841
HpyF3I	CTNAG	5 cut(s)	396, 419, 672, 917, 1079
HpySE526I	ACGT	2 cut(s)	861, 926
HspAI	GCGC	2 cut(s)	197, 287
Kpn2I	TCCGGA	1 cut(s)	1053
Kzo9I	GATC	4 cut(s)	229, 525, 538, 871
LmnI	GCTCC	7 cut(s)	18, 40, 194, 362, 373, 465, 673
Lsp1109I	GCAGC	8 cut(s)	103, 136, 271, 799, 808, 811, 1028, 1031
MaeII	ACGT	2 cut(s)	861, 926
MaeIII	GTNAC	6 cut(s)	391, 414, 622, 688, 957, 1025
MalI	GATC	4 cut(s)	231, 527, 540, 873
MbiI	CCGCTC	1 cut(s)	21
MboI	GATC	4 cut(s)	229, 525, 538, 871
MboII	GAAGA	6 cut(s)	44, 287, 626, 721, 917, 955
MflI	RGATCY	1 cut(s)	538
MhlI	GDGCHC	2 cut(s)	462, 1115
MlsI	TGGCCA	1 cut(s)	470
MluCI	AATT	9 cut(s)	142, 161, 183, 234, 476, 553, 764, 802, 1043
MluNI	TGGCCA	1 cut(s)	470
Mox20I	TGGCCA	1 cut(s)	470
MroI	TCCGGA	1 cut(s)	1053
MscI	TGGCCA	1 cut(s)	470
MseI	TTAA	3 cut(s)	315, 351, 474
MslI	CAYNNNNRTG	2 cut(s)	519, 944
Msp20I	TGGCCA	1 cut(s)	470
MspA1I	CMGCKG	2 cut(s)	127, 660
MspI	CCGG	1 cut(s)	1054
MspR9I	CCNGG	2 cut(s)	408, 701
MvaI	CCWGG	2 cut(s)	408, 701
MvnI	CGCG	1 cut(s)	328
MwoI	GCNNNNNNNGC	3 cut(s)	818, 821, 841
NdeII	GATC	4 cut(s)	229, 525, 538, 871
NlaIV	GGNNCC	3 cut(s)	156, 375, 669
NmuCI	GTSAC	1 cut(s)	414
NruI	TCGCGA	1 cut(s)	328
NspI	RCATGY	5 cut(s)	518, 584, 784, 799, 949
NspV	TTCGAA	1 cut(s)	789
PaeI	GCATGC	2 cut(s)	784, 949
PceI	AGGCCT	2 cut(s)	844, 922
PciI	ACATGT	1 cut(s)	580
PcsI	WCGNNNNNNNCGW	1 cut(s)	304
PfeI	GAWTC	1 cut(s)	343
PkrI	GCNGC	9 cut(s)	23, 118, 126, 286, 814, 823, 826, 1018, 1021
PscI	ACATGT	1 cut(s)	580
Psp124BI	GAGCTC	2 cut(s)	462, 1115
Psp6I	CCWGG	2 cut(s)	406, 699
PspFI	CCCAGC	1 cut(s)	120
PspGI	CCWGG	2 cut(s)	406, 699
PspN4I	GGNNCC	3 cut(s)	156, 375, 669
PspPI	GGNCC	2 cut(s)	154, 1104
PstNI	CAGNNNCTG	3 cut(s)	127, 647, 1001
PsuI	RGATCY	1 cut(s)	538
PvuII	CAGCTG	1 cut(s)	127
RruI	TCGCGA	1 cut(s)	328
RsaI	GTAC	2 cut(s)	224, 428
RsaNI	GTAC	2 cut(s)	223, 427
RseI	CAYNNNNRTG	2 cut(s)	519, 944
SacI	GAGCTC	2 cut(s)	462, 1115
SaqAI	TTAA	3 cut(s)	315, 351, 474
SatI	GCNGC	9 cut(s)	22, 117, 125, 285, 813, 822, 825, 1017, 1020
Sau3AI	GATC	4 cut(s)	229, 525, 538, 871
Sau96I	GGNCC	2 cut(s)	154, 1104
ScrFI	CCNGG	2 cut(s)	408, 701
SduI	GDGCHC	2 cut(s)	462, 1115
SfuI	TTCGAA	1 cut(s)	789
SinI	GGWCC	2 cut(s)	154, 1104
SmiMI	CAYNNNNRTG	2 cut(s)	519, 944
SmlI	CTYRAG	1 cut(s)	290
SmoI	CTYRAG	1 cut(s)	290
SphI	GCATGC	2 cut(s)	784, 949
Sse9I	AATT	9 cut(s)	142, 161, 183, 234, 476, 553, 764, 802, 1043
SseBI	AGGCCT	2 cut(s)	844, 922
SsiI	CCGC	8 cut(s)	21, 24, 53, 658, 778, 854, 943, 986
SstI	GAGCTC	2 cut(s)	462, 1115
StuI	AGGCCT	2 cut(s)	844, 922
StyD4I	CCNGG	2 cut(s)	406, 699
TaaI	ACNGT	2 cut(s)	688, 1089
TaiI	ACGT	2 cut(s)	864, 929
TaqI	TCGA	3 cut(s)	81, 135, 789
TasI	AATT	9 cut(s)	142, 161, 183, 234, 476, 553, 764, 802, 1043
TauI	GCSGC	1 cut(s)	24
TfiI	GAWTC	1 cut(s)	343
Tru1I	TTAA	3 cut(s)	315, 351, 474
Tru9I	TTAA	3 cut(s)	315, 351, 474
TscAI	CASTG	1 cut(s)	1006
TseFI	GTSAC	1 cut(s)	414
TseI	GCWGC	8 cut(s)	116, 124, 284, 812, 821, 824, 1016, 1019
Tsp45I	GTSAC	1 cut(s)	414
TspDTI	ATGAA	3 cut(s)	160, 228, 346
TspGWI	ACGGA	2 cut(s)	240, 1044
TspRI	CASTG	1 cut(s)	1006
VpaK11BI	GGWCC	2 cut(s)	154, 1104
XagI	CCTNNNNNAGG	1 cut(s)	90
XapI	RAATTY	2 cut(s)	476, 1043
XceI	RCATGY	5 cut(s)	518, 584, 784, 799, 949

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.