MD12G1190300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007877
Physical Location & Seq
Reverse (-)
27311553 .. 27314130
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1190300.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGTGTGACCGTCTTCCCTCCCTCCCCCTATCTTTTCCTCTCACAAAATCTCCAAAATTAGGGTTTACCAGAACGTATTGGAGCTCCCGTATCCAACCGCATCCCTACTTTTACCCGCCAAATTGCTCGAAACCATCCAATTTCACGAATTCAGAGCTCACAAACTCCAGAAATGGGAGGTCTTTGTTCCTCAGCAGAAGGGCCAGCGAGAATCCGATAGCTGATTTTGGGATTCCGGCCGATTTAGGGTTTAAGGAGAAAGGGGAAAACCTCTTCAACCTCGAAAGTGAGGCAATTGAGTCAGTAGCGCGACATGGGATTGATCCCAGTGAGGAATCCAATGCTTCATTGGGTTCAGAAAATTTTCTGGAATCGGAGGGCGGTTCGGGGGAGGAGAACCGAAATCGGAAGAGTAATGAGAGCGTAAAAGACGAGAATCAGGTCCGAATGGCGGGTGCGGACGGTGATGGGTTGGAGAAGGAGGATAATTTGAAGCGCATTGATGAAAAAGTTGGACTAAGAAAAGGGCGGCAGGTAATCAGGAGGTCCAATATTCTTGCAAAGCAAGTTATCTGTATTCAAAGTGCTCTCAGCTTGGGATTTGTCTCTCAGCTTTGGGTGAATACCAATTCATGGATGGTAAAGTTTGTAGAAGTGAGGCGAAACTTGCTTTCGGGAGAGTTTGAACGTTTTCTTCTCGACGATATTACCCAGGTTGGTGATGTTGTGCTTGTCGAGGACGAGAGTGTGATTGATGATGATGAATTTAAGATTGTTGGACTGGAAACGTTGGTAGGATACCAAGTAGTAACACCAGGTCGACGAACAATTGGAAAGGTGCGGGGATACAGTTTTAACATCAATTCAGGGGCTGTCGAGTCTCTTGAACTTGATTCATTTGGGATTTCCTATATTCCATCAAGTTTGGTGAGTACCTATGCGCTGTTTGTTGAGGATGTGCTGGAAGTTGTGTCTGATGCTGTCATTGTGCATGAAGCTGCAGCTTCGCGCATACACAGGCTGACCAAGGGTCTCTTGGACAGCCAAAATGTTGCAAGCTCTATAGATGATCTCGACGAGTATTCCGACATTGAAAGACCTGTAAGATCGGATAAGTATACAAGAAGAAGAAATTTTGGTGACCAGAAGTTCAATTCCAGGAAAAGCAAGACCAATGGCAATGATGATTGGGACCTCCCAATGGATTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRC

Protein Analysis

405

Amino Acids

45.35

Weight (kDa)

5.05

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccB7I CCANNNNNTGG 1 cut(s) 633
AccI GTMKAC 2 cut(s) 820, 1118
AccII CGCG 2 cut(s) 310, 1009
AciI CCGC 7 cut(s) 98, 116, 381, 452, 458, 529, 841
AclI AACGTT 2 cut(s) 688, 788
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 4 cut(s) 148, 361, 764, 1132
AfaI GTAC 1 cut(s) 934
AfiI CCNNNNNNNGG 6 cut(s) 59, 174, 246, 451, 633, 1201
AgsI TTSAA 7 cut(s) 277, 493, 581, 686, 887, 1094, 1153
AhdI GACNNNNNGTC 1 cut(s) 1029
AjnI CCWGG 3 cut(s) 711, 814, 1157
AluBI AGCT 8 cut(s) 84, 157, 221, 594, 613, 998, 1004, 1059
AluI AGCT 8 cut(s) 84, 157, 221, 594, 613, 998, 1004, 1059
Alw21I GWGCWC 3 cut(s) 86, 159, 589
Alw26I GTCTC 3 cut(s) 610, 885, 1037
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 872
AoxI GGCC 2 cut(s) 201, 237
ApeKI GCWGC 2 cut(s) 998, 1001
ApoI RAATTY 4 cut(s) 148, 361, 764, 1132
Asp700I GAANNNNTTC 2 cut(s) 363, 690
AspLEI GCGC 4 cut(s) 310, 498, 943, 1011
AspS9I GGNCC 4 cut(s) 201, 442, 546, 1192
AsuHPI GGTGA 5 cut(s) 476, 631, 731, 940, 1151
AvaII GGWCC 3 cut(s) 442, 546, 1192
BanII GRGCYC 2 cut(s) 86, 159
BbsI GAAGAC 1 cut(s) 5
Bbv12I GWGCWC 3 cut(s) 86, 159, 589
BbvCI CCTCAGC 1 cut(s) 191
BbvI GCAGC 2 cut(s) 985, 1013
BccI CCATC 4 cut(s) 142, 461, 631, 925
BciT130I CCWGG 3 cut(s) 713, 816, 1159
BciVI GTATCC 3 cut(s) 101, 791, 839
BcoDI GTCTC 3 cut(s) 610, 885, 1037
BfmI CTRYAG 2 cut(s) 999, 1062
BfuAI ACCTGC 1 cut(s) 523
BfuI GTATCC 3 cut(s) 101, 791, 839
BisI GCNGC 3 cut(s) 530, 999, 1002
BlsI GCNGC 3 cut(s) 531, 1000, 1003
Bme1390I CCNGG 3 cut(s) 713, 816, 1159
Bme18I GGWCC 3 cut(s) 442, 546, 1192
BmeRI GACNNNNNGTC 1 cut(s) 1029
BmgT120I GGNCC 4 cut(s) 201, 442, 546, 1192
BmiI GGNNCC 1 cut(s) 1193
BmrFI CCNGG 3 cut(s) 713, 816, 1159
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 109, 967
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 5
BpmI CTGGAG 1 cut(s) 151
Bpu10I CCTNAGC 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 2 cut(s) 711, 1026
BsaXI ACNNNNNCTCC 4 cut(s) 34, 64, 73, 103
Bsc4I CCNNNNNNNGG 6 cut(s) 59, 174, 246, 451, 633, 1201
Bse1I ACTGG 2 cut(s) 327, 786
Bse3DI GCAATG 1 cut(s) 1186
BseBI CCWGG 3 cut(s) 713, 816, 1159
BseDI CCNNGG 2 cut(s) 711, 1026
BseGI GGATG 4 cut(s) 100, 134, 642, 961
BseLI CCNNNNNNNGG 6 cut(s) 59, 174, 246, 451, 633, 1201
BseMI GCAATG 1 cut(s) 1186
BseMII CTCAG 3 cut(s) 205, 604, 623
BseNI ACTGG 2 cut(s) 327, 786
BseRI GAGGAG 1 cut(s) 407
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 985, 1013
Bsh1236I CGCG 2 cut(s) 310, 1009
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 2 cut(s) 203, 239
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 3 cut(s) 86, 159, 589
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 1205
BslI CCNNNNNNNGG 6 cut(s) 59, 174, 246, 451, 633, 1201
BsmAI GTCTC 3 cut(s) 610, 885, 1037
BsmFI GGGAC 1 cut(s) 1205
BsnI GGCC 2 cut(s) 203, 239
Bso31I GGTCTC 1 cut(s) 1037
Bsp1286I GDGCHC 3 cut(s) 86, 159, 589
Bsp143I GATC 3 cut(s) 322, 1069, 1106
BspACI CCGC 7 cut(s) 98, 116, 381, 452, 458, 529, 841
BspANI GGCC 2 cut(s) 203, 239
BspCNI CTCAG 3 cut(s) 204, 603, 622
BspFNI CGCG 2 cut(s) 310, 1009
BspLI GGNNCC 1 cut(s) 1193
BspMAI CTGCAG 1 cut(s) 1003
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 1037
BsrDI GCAATG 1 cut(s) 1186
BsrI ACTGG 2 cut(s) 327, 786
BssECI CCNNGG 2 cut(s) 711, 1026
BssMI GATC 3 cut(s) 322, 1069, 1106
BssNAI GTATAC 1 cut(s) 1119
BssT1I CCWWGG 1 cut(s) 1026
Bst1107I GTATAC 1 cut(s) 1119
Bst2UI CCWGG 3 cut(s) 713, 816, 1159
Bst4CI ACNGT 3 cut(s) 11, 464, 851
Bst6I CTCTTC 2 cut(s) 278, 404
BstC8I GCNNGC 2 cut(s) 205, 1057
BstDEI CTNAG 4 cut(s) 191, 518, 590, 609
BstEII GGTNACC 1 cut(s) 1139
BstF5I GGATG 4 cut(s) 100, 134, 642, 961
BstFNI CGCG 2 cut(s) 310, 1009
BstHHI GCGC 4 cut(s) 310, 498, 943, 1011
BstKTI GATC 3 cut(s) 325, 1072, 1109
BstMAI GTCTC 3 cut(s) 610, 885, 1037
BstMBI GATC 3 cut(s) 322, 1069, 1106
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 1 cut(s) 667
BstNI CCWGG 3 cut(s) 713, 816, 1159
BstPI GGTNACC 1 cut(s) 1139
BstSCI CCNGG 3 cut(s) 711, 814, 1157
BstSFI CTRYAG 2 cut(s) 999, 1062
BstUI CGCG 2 cut(s) 310, 1009
BstV1I GCAGC 2 cut(s) 985, 1013
BstV2I GAAGAC 1 cut(s) 5
BstZ17I GTATAC 1 cut(s) 1119
BstZI CGGCCG 1 cut(s) 237
BsuI GTATCC 3 cut(s) 101, 791, 839
BsuRI GGCC 2 cut(s) 203, 239
BtsCI GGATG 4 cut(s) 100, 134, 642, 961
BtsIMutI CAGTG 1 cut(s) 334
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 2 cut(s) 205, 1057
CaiI CAGNNNCTG 1 cut(s) 872
CfoI GCGC 4 cut(s) 310, 498, 943, 1011
Cfr13I GGNCC 4 cut(s) 201, 442, 546, 1192
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 1 cut(s) 933
CviAII CATG 3 cut(s) 314, 633, 992
CviQI GTAC 1 cut(s) 933
DdeI CTNAG 4 cut(s) 191, 518, 590, 609
DpnI GATC 3 cut(s) 324, 1071, 1108
DpnII GATC 3 cut(s) 322, 1069, 1106
DriI GACNNNNNGTC 1 cut(s) 1029
EaeI YGGCCR 1 cut(s) 237
EagI CGGCCG 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 278, 404
Eam1105I GACNNNNNGTC 1 cut(s) 1029
EarI CTCTTC 2 cut(s) 278, 404
Ecl136II GAGCTC 2 cut(s) 84, 157
EclXI CGGCCG 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 1026
Eco24I GRGCYC 2 cut(s) 86, 159
Eco31I GGTCTC 1 cut(s) 1037
Eco47I GGWCC 3 cut(s) 442, 546, 1192
Eco52I CGGCCG 1 cut(s) 237
Eco53kI GAGCTC 2 cut(s) 84, 157
Eco91I GGTNACC 1 cut(s) 1139
EcoICRI GAGCTC 2 cut(s) 84, 157
EcoO109I RGGNCCY 1 cut(s) 1192
EcoO65I GGTNACC 1 cut(s) 1139
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 3 cut(s) 711, 814, 1157
EcoT14I CCWWGG 1 cut(s) 1026
EcoT38I GRGCYC 2 cut(s) 86, 159
ErhI CCWWGG 1 cut(s) 1026
FaeI CATG 3 cut(s) 317, 636, 995
FaiI YATR 8 cut(s) 315, 634, 912, 939, 993, 1013, 1064, 1119
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 1 cut(s) 1205
FatI CATG 3 cut(s) 313, 632, 991
FauI CCCGC 3 cut(s) 123, 445, 834
FblI GTMKAC 2 cut(s) 820, 1118
Fnu4HI GCNGC 3 cut(s) 530, 999, 1002
FokI GGATG 4 cut(s) 87, 121, 649, 968
FriOI GRGCYC 2 cut(s) 86, 159
Fsp4HI GCNGC 3 cut(s) 530, 999, 1002
GlaI GCGC 4 cut(s) 309, 497, 942, 1010
GluI GCNGC 3 cut(s) 530, 999, 1002
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 2 cut(s) 203, 239
HapII CCGG 1 cut(s) 236
HhaI GCGC 4 cut(s) 310, 498, 943, 1011
Hin1II CATG 3 cut(s) 317, 636, 995
Hin6I GCGC 4 cut(s) 308, 496, 941, 1009
HinP1I GCGC 4 cut(s) 308, 496, 941, 1009
HincII GTYRAC 1 cut(s) 821
HindII GTYRAC 1 cut(s) 821
HinfI GANTC 8 cut(s) 211, 232, 299, 335, 371, 436, 878, 893
HpaII CCGG 1 cut(s) 236
HphI GGTGA 5 cut(s) 476, 631, 731, 940, 1151
Hpy166II GTNNAC 3 cut(s) 66, 821, 1119
Hpy188III TCNNGA 8 cut(s) 145, 168, 368, 541, 675, 698, 884, 1073
Hpy8I GTNNAC 3 cut(s) 66, 821, 1119
Hpy99I CGWCG 3 cut(s) 704, 825, 1079
HpyAV CCTTC 2 cut(s) 192, 472
HpyCH4III ACNGT 3 cut(s) 11, 464, 851
HpyCH4IV ACGT 3 cut(s) 74, 688, 788
HpyCH4V TGCA 4 cut(s) 560, 991, 1001, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 667
HpyF3I CTNAG 4 cut(s) 191, 518, 590, 609
HpySE526I ACGT 3 cut(s) 74, 688, 788
Hsp92II CATG 3 cut(s) 317, 636, 995
HspAI GCGC 4 cut(s) 308, 496, 941, 1009
Kzo9I GATC 3 cut(s) 322, 1069, 1106
LmnI GCTCC 2 cut(s) 81, 89
Lsp1109I GCAGC 2 cut(s) 985, 1013
LweI GCATC 2 cut(s) 109, 967
MabI ACCWGGT 1 cut(s) 814
MaeII ACGT 3 cut(s) 74, 688, 788
MaeIII GTNAC 3 cut(s) 5, 808, 1139
MalI GATC 3 cut(s) 324, 1071, 1108
MboI GATC 3 cut(s) 322, 1069, 1106
MboII GAAGA 6 cut(s) 5, 265, 421, 686, 1137, 1140
MfeI CAATTG 2 cut(s) 294, 828
MhlI GDGCHC 3 cut(s) 86, 159, 589
MlyI GAGTC 2 cut(s) 308, 887
MmeI TCCRAC 5 cut(s) 118, 453, 493, 757, 1110
MroXI GAANNNNTTC 2 cut(s) 363, 690
MseI TTAA 3 cut(s) 252, 768, 855
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 3 cut(s) 713, 816, 1159
MunI CAATTG 2 cut(s) 294, 828
MvaI CCWGG 3 cut(s) 713, 816, 1159
MvnI CGCG 2 cut(s) 310, 1009
MwoI GCNNNNNNNGC 1 cut(s) 667
NdeII GATC 3 cut(s) 322, 1069, 1106
NlaIII CATG 3 cut(s) 317, 636, 995
NlaIV GGNNCC 1 cut(s) 1193
NmuCI GTSAC 2 cut(s) 5, 1139
PcsI WCGNNNNNNNCGW 1 cut(s) 1083
PdmI GAANNNNTTC 2 cut(s) 363, 690
PfeI GAWTC 6 cut(s) 211, 232, 335, 371, 436, 893
PflMI CCANNNNNTGG 1 cut(s) 633
PfoI TCCNGGA 1 cut(s) 1157
PkrI GCNGC 3 cut(s) 531, 1000, 1003
PleI GAGTC 2 cut(s) 307, 886
PpsI GAGTC 2 cut(s) 307, 886
PpuMI RGGWCCY 1 cut(s) 1192
Psp124BI GAGCTC 2 cut(s) 86, 159
Psp1406I AACGTT 2 cut(s) 688, 788
Psp5II RGGWCCY 1 cut(s) 1192
Psp6I CCWGG 3 cut(s) 711, 814, 1157
PspEI GGTNACC 1 cut(s) 1139
PspGI CCWGG 3 cut(s) 711, 814, 1157
PspN4I GGNNCC 1 cut(s) 1193
PspPI GGNCC 4 cut(s) 201, 442, 546, 1192
PspPPI RGGWCCY 1 cut(s) 1192
PstI CTGCAG 1 cut(s) 1003
PstNI CAGNNNCTG 1 cut(s) 872
RsaI GTAC 1 cut(s) 934
RsaNI GTAC 1 cut(s) 933
SacI GAGCTC 2 cut(s) 86, 159
SalI GTCGAC 1 cut(s) 819
SaqAI TTAA 3 cut(s) 252, 768, 855
SatI GCNGC 3 cut(s) 530, 999, 1002
Sau3AI GATC 3 cut(s) 322, 1069, 1106
Sau96I GGNCC 4 cut(s) 201, 442, 546, 1192
SchI GAGTC 2 cut(s) 308, 887
ScrFI CCNGG 3 cut(s) 713, 816, 1159
SduI GDGCHC 3 cut(s) 86, 159, 589
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 2 cut(s) 109, 967
SfcI CTRYAG 2 cut(s) 999, 1062
SinI GGWCC 3 cut(s) 442, 546, 1192
SsiI CCGC 7 cut(s) 98, 116, 381, 452, 458, 529, 841
SspI AATATT 1 cut(s) 553
SstI GAGCTC 2 cut(s) 86, 159
StyD4I CCNGG 3 cut(s) 711, 814, 1157
StyI CCWWGG 1 cut(s) 1026
TaaI ACNGT 3 cut(s) 11, 464, 851
TaiI ACGT 3 cut(s) 77, 691, 791
TaqI TCGA 7 cut(s) 128, 282, 699, 735, 820, 876, 1074
TauI GCSGC 1 cut(s) 532
TfiI GAWTC 6 cut(s) 211, 232, 335, 371, 436, 893
Tru1I TTAA 3 cut(s) 252, 768, 855
Tru9I TTAA 3 cut(s) 252, 768, 855
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 5, 1139
TseI GCWGC 2 cut(s) 998, 1001
Tsp45I GTSAC 2 cut(s) 5, 1139
TspDTI ATGAA 6 cut(s) 336, 519, 621, 777, 885, 1008
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 1 cut(s) 633
VpaK11BI GGWCC 3 cut(s) 442, 546, 1192
XapI RAATTY 4 cut(s) 148, 361, 764, 1132
XcmI CCANNNNNNNNNTGG 3 cut(s) 346, 634, 1033
XmiI GTMKAC 2 cut(s) 820, 1118
XmnI GAANNNNTTC 2 cut(s) 363, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.