pycom04g15520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007877
Physical Location & Seq
Reverse (-)
18392238 .. 18394552
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15520.1

Sequence Viewer

Length: 1206 bp
ATGTGCGAGCGTCTTCCCTCCAGCCCCCTATCTTTTCCGCTCACAAAATCTCCAAAATTAGGGTTTACCAGAACCAATTGGAGCTCCCAAATCCAACCGCACCCCAACTTTTACACCCCAAATTGTTCGAAGCTATCGAATTTCGCGAATTCAGAAATCACAAATTCCAGAAATGGGAGGTCTTTGTTCCTCAGCAGAAGGGCCAGCGATAATCCGAAAGCTGATTTTGGGATTCCGGCCGAATTAGGGTTTAAGGAGAAAGGTGAAAACCTCTTCGACCTCGCAACAGAATCAATTGAGTCAGTAACGCGAAATGGGATTGATCCCAGGGAGGAATCCAATGCTTCATTGGGTTCAGAAAATTTATTGGAATCGGAGGGCGGTTCGGGGGAGGATAAGCGAAATCGGAAGAGCAACGAGAGCGTAAAAGAGGAGAATCGGGTTCGAATTGAGGGTACGGATGGTGATGGGTTGGAGAAGGAGGAGAATTTGAAGCATATTGATGAAAAAGTTGGGCTGAGAAAAGGGAGGCAGGTGATGAGGAGGTCCAATATTCTTGCGAAGCAAGTTATCAGTATCCGAAGTGCGCTCAGCTTGGGATTTGTCTCGCAGCTTTGGGTCAATACCAACTCTTGGATGGTAATGTTTGTAGAAGTGAGGCGAAACCTGCTTTCGGGGGATTTCGAACGTTTTCTTCTCGATGATATTACCCAGGTTGGTGATGTTGTGCTTGTCAAGGACGAGAGTGTGATTGAAGACGAATTTAAGATTGTTGGACTGGAAACGTTGGTAGGATACCAAGTTATAACACCAGGCCGACGAATGATTGGAAAGGTGCGGGGGTACAGTTTTAACGTCAATTCAGGGGCTGTAGAATCCCTTGAATTTGATTCATTTGGAATTTCCTATATCCCATCAAGTTTGGTGAGTACCTATGCGTTGTTTGTTGAGGACGTGCTGGAAGTTGTCTCTGATGCCGTCATTGTGCACGAAGCTGCAGCTTCCCGCATACACAGGCTGACCAAGGGTTTCTTGGACGGCCGAAATGTTGCAACCTCCATAGATGATCTCGATGAATATTCCGACTTTGAAAGACCCGTAAGATCGGATAAGCATACAAGAAGAAGAAATTTTGGTAACCAGAAGTTCGATTCCAGGAAAAGCAAGACCAATGATGATTGGGACCTCCCAATGGATTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRC

Protein Analysis

402

Amino Acids

45.2

Weight (kDa)

5.45

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AarI CACCTGC 1 cut(s) 523
Acc36I ACCTGC 2 cut(s) 523, 675
AccB7I CCANNNNNTGG 1 cut(s) 633
AccBSI CCGCTC 1 cut(s) 40
AccII CGCG 2 cut(s) 146, 310
AciI CCGC 5 cut(s) 38, 98, 381, 838, 1006
AclI AACGTT 2 cut(s) 688, 785
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 2 cut(s) 237, 1039
AcsI RAATTY 9 cut(s) 139, 148, 163, 361, 487, 761, 884, 900, 1129
AfaI GTAC 3 cut(s) 457, 845, 931
AfiI CCNNNNNNNGG 6 cut(s) 59, 174, 246, 633, 673, 1192
AgsI TTSAA 4 cut(s) 493, 755, 884, 1091
AjiI CACGTC 1 cut(s) 955
AjnI CCWGG 4 cut(s) 326, 711, 811, 1154
AluBI AGCT 7 cut(s) 84, 133, 221, 594, 613, 995, 1001
AluI AGCT 7 cut(s) 84, 133, 221, 594, 613, 995, 1001
Alw21I GWGCWC 2 cut(s) 86, 990
Alw26I GTCTC 2 cut(s) 610, 973
Alw44I GTGCAC 1 cut(s) 986
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 869
AoxI GGCC 4 cut(s) 201, 237, 814, 1039
ApaLI GTGCAC 1 cut(s) 986
ApeKI GCWGC 3 cut(s) 610, 995, 998
ApoI RAATTY 9 cut(s) 139, 148, 163, 361, 487, 761, 884, 900, 1129
Asp700I GAANNNNTTC 1 cut(s) 690
AspLEI GCGC 1 cut(s) 589
AspS9I GGNCC 3 cut(s) 201, 546, 1183
AsuHPI GGTGA 5 cut(s) 275, 476, 547, 731, 937
AsuII TTCGAA 3 cut(s) 128, 445, 684
AvaII GGWCC 2 cut(s) 546, 1183
BaeGI GKGCMC 1 cut(s) 990
BanII GRGCYC 1 cut(s) 86
BbsI GAAGAC 2 cut(s) 5, 762
Bbv12I GWGCWC 2 cut(s) 86, 990
BbvCI CCTCAGC 1 cut(s) 191
BbvI GCAGC 3 cut(s) 622, 982, 1010
BccI CCATC 4 cut(s) 455, 461, 631, 922
BceAI ACGGC 2 cut(s) 962, 1054
BciT130I CCWGG 4 cut(s) 328, 713, 813, 1156
BciVI GTATCC 2 cut(s) 587, 788
BcoDI GTCTC 2 cut(s) 610, 973
BfmI CTRYAG 2 cut(s) 870, 996
BfuAI ACCTGC 2 cut(s) 523, 675
BfuI GTATCC 2 cut(s) 587, 788
BisI GCNGC 3 cut(s) 611, 996, 999
BlpI GCTNAGC 1 cut(s) 590
BlsI GCNGC 3 cut(s) 612, 997, 1000
Bme1390I CCNGG 4 cut(s) 328, 713, 813, 1156
Bme18I GGWCC 2 cut(s) 546, 1183
BmgBI CACGTC 1 cut(s) 955
BmgT120I GGNCC 3 cut(s) 201, 546, 1183
BmiI GGNNCC 1 cut(s) 1184
BmrFI CCNGG 4 cut(s) 328, 713, 813, 1156
BmsI GCATC 1 cut(s) 964
BpiI GAAGAC 2 cut(s) 5, 762
BpmI CTGGAG 1 cut(s) 4
Bpu10I CCTNAGC 1 cut(s) 191
Bpu1102I GCTNAGC 1 cut(s) 590
Bpu14I TTCGAA 3 cut(s) 128, 445, 684
BsaJI CCNNGG 4 cut(s) 326, 327, 711, 1023
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 6 cut(s) 59, 174, 246, 633, 673, 1192
Bse1I ACTGG 1 cut(s) 783
BseBI CCWGG 4 cut(s) 328, 713, 813, 1156
BseDI CCNNGG 4 cut(s) 326, 327, 711, 1023
BseGI GGATG 2 cut(s) 466, 642
BseLI CCNNNNNNNGG 6 cut(s) 59, 174, 246, 633, 673, 1192
BseMII CTCAG 3 cut(s) 205, 509, 604
BseNI ACTGG 1 cut(s) 783
BseRI GAGGAG 3 cut(s) 446, 497, 556
BseSI GKGCMC 1 cut(s) 990
BseX3I CGGCCG 2 cut(s) 237, 1039
BseXI GCAGC 3 cut(s) 622, 982, 1010
Bsh1236I CGCG 2 cut(s) 146, 310
Bsh1285I CGRYCG 2 cut(s) 240, 1042
BshFI GGCC 4 cut(s) 203, 239, 816, 1041
BsiEI CGRYCG 2 cut(s) 240, 1042
BsiHKAI GWGCWC 2 cut(s) 86, 990
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 1196
BslI CCNNNNNNNGG 6 cut(s) 59, 174, 246, 633, 673, 1192
BsmAI GTCTC 2 cut(s) 610, 973
BsmFI GGGAC 1 cut(s) 1196
BsnI GGCC 4 cut(s) 203, 239, 816, 1041
Bsp119I TTCGAA 3 cut(s) 128, 445, 684
Bsp1286I GDGCHC 2 cut(s) 86, 990
Bsp143I GATC 3 cut(s) 322, 1066, 1103
Bsp1720I GCTNAGC 1 cut(s) 590
Bsp68I TCGCGA 1 cut(s) 146
BspACI CCGC 5 cut(s) 38, 98, 381, 838, 1006
BspANI GGCC 4 cut(s) 203, 239, 816, 1041
BspCNI CTCAG 3 cut(s) 204, 510, 603
BspFNI CGCG 2 cut(s) 146, 310
BspLI GGNNCC 1 cut(s) 1184
BspMAI CTGCAG 1 cut(s) 1000
BspMI ACCTGC 2 cut(s) 523, 675
BspPI GGATC 1 cut(s) 317
BspQI GCTCTTC 1 cut(s) 404
BspT104I TTCGAA 3 cut(s) 128, 445, 684
BsrBI CCGCTC 1 cut(s) 40
BsrI ACTGG 1 cut(s) 783
BssECI CCNNGG 4 cut(s) 326, 327, 711, 1023
BssMI GATC 3 cut(s) 322, 1066, 1103
BssT1I CCWWGG 1 cut(s) 1023
Bst2UI CCWGG 4 cut(s) 328, 713, 813, 1156
Bst4CI ACNGT 1 cut(s) 848
Bst6I CTCTTC 2 cut(s) 278, 404
BstBI TTCGAA 3 cut(s) 128, 445, 684
BstC8I GCNNGC 2 cut(s) 8, 205
BstDEI CTNAG 3 cut(s) 191, 518, 590
BstEII GGTNACC 1 cut(s) 1136
BstF5I GGATG 2 cut(s) 466, 642
BstFNI CGCG 2 cut(s) 146, 310
BstHHI GCGC 1 cut(s) 589
BstKTI GATC 3 cut(s) 325, 1069, 1106
BstMAI GTCTC 2 cut(s) 610, 973
BstMBI GATC 3 cut(s) 322, 1066, 1103
BstMCI CGRYCG 2 cut(s) 240, 1042
BstMWI GCNNNNNNNGC 2 cut(s) 420, 667
BstNI CCWGG 4 cut(s) 328, 713, 813, 1156
BstPI GGTNACC 1 cut(s) 1136
BstSCI CCNGG 4 cut(s) 326, 711, 811, 1154
BstSFI CTRYAG 2 cut(s) 870, 996
BstSLI GKGCMC 1 cut(s) 990
BstUI CGCG 2 cut(s) 146, 310
BstV1I GCAGC 3 cut(s) 622, 982, 1010
BstV2I GAAGAC 2 cut(s) 5, 762
BstZI CGGCCG 2 cut(s) 237, 1039
BsuI GTATCC 2 cut(s) 587, 788
BsuRI GGCC 4 cut(s) 203, 239, 816, 1041
BtrI CACGTC 1 cut(s) 955
BtsCI GGATG 2 cut(s) 466, 642
BtuMI TCGCGA 1 cut(s) 146
BveI ACCTGC 2 cut(s) 523, 675
Cac8I GCNNGC 2 cut(s) 8, 205
CaiI CAGNNNCTG 1 cut(s) 869
CfoI GCGC 1 cut(s) 589
Cfr13I GGNCC 3 cut(s) 201, 546, 1183
Csp6I GTAC 3 cut(s) 456, 844, 930
CviQI GTAC 3 cut(s) 456, 844, 930
DdeI CTNAG 3 cut(s) 191, 518, 590
DpnI GATC 3 cut(s) 324, 1068, 1105
DpnII GATC 3 cut(s) 322, 1066, 1103
EaeI YGGCCR 2 cut(s) 237, 1039
EagI CGGCCG 2 cut(s) 237, 1039
Eam1104I CTCTTC 2 cut(s) 278, 404
EarI CTCTTC 2 cut(s) 278, 404
Ecl136II GAGCTC 1 cut(s) 84
EclXI CGGCCG 2 cut(s) 237, 1039
Eco130I CCWWGG 1 cut(s) 1023
Eco24I GRGCYC 1 cut(s) 86
Eco47I GGWCC 2 cut(s) 546, 1183
Eco52I CGGCCG 2 cut(s) 237, 1039
Eco53kI GAGCTC 1 cut(s) 84
Eco91I GGTNACC 1 cut(s) 1136
EcoICRI GAGCTC 1 cut(s) 84
EcoO109I RGGNCCY 1 cut(s) 1183
EcoO65I GGTNACC 1 cut(s) 1136
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 4 cut(s) 326, 711, 811, 1154
EcoT14I CCWWGG 1 cut(s) 1023
EcoT38I GRGCYC 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 1023
FaiI YATR 7 cut(s) 498, 806, 909, 936, 1010, 1061, 1116
FalI AAGNNNNNCTT 2 cut(s) 1016, 1048
FaqI GGGAC 1 cut(s) 1196
FauI CCCGC 2 cut(s) 831, 1013
Fnu4HI GCNGC 3 cut(s) 611, 996, 999
FokI GGATG 2 cut(s) 473, 649
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 3 cut(s) 611, 996, 999
GlaI GCGC 1 cut(s) 588
GluI GCNGC 3 cut(s) 611, 996, 999
GsuI CTGGAG 1 cut(s) 4
HaeIII GGCC 4 cut(s) 203, 239, 816, 1041
HapII CCGG 1 cut(s) 236
HhaI GCGC 1 cut(s) 589
Hin6I GCGC 1 cut(s) 587
HinP1I GCGC 1 cut(s) 587
HinfI GANTC 9 cut(s) 232, 290, 299, 335, 371, 436, 875, 890, 1151
HpaII CCGG 1 cut(s) 236
HphI GGTGA 5 cut(s) 275, 476, 547, 731, 937
Hpy166II GTNNAC 2 cut(s) 66, 988
Hpy188III TCNNGA 4 cut(s) 145, 168, 698, 1070
Hpy8I GTNNAC 2 cut(s) 66, 988
Hpy99I CGWCG 1 cut(s) 822
HpyAV CCTTC 2 cut(s) 192, 472
HpyCH4III ACNGT 1 cut(s) 848
HpyCH4IV ACGT 4 cut(s) 688, 785, 855, 954
HpyCH4V TGCA 3 cut(s) 988, 998, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 420, 667
HpyF3I CTNAG 3 cut(s) 191, 518, 590
HpySE526I ACGT 4 cut(s) 688, 785, 855, 954
HspAI GCGC 1 cut(s) 587
Kzo9I GATC 3 cut(s) 322, 1066, 1103
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 2 cut(s) 81, 89
Lsp1109I GCAGC 3 cut(s) 622, 982, 1010
LweI GCATC 1 cut(s) 964
MaeII ACGT 4 cut(s) 688, 785, 855, 954
MaeIII GTNAC 2 cut(s) 304, 1136
MalI GATC 3 cut(s) 324, 1068, 1105
MbiI CCGCTC 1 cut(s) 40
MboI GATC 3 cut(s) 322, 1066, 1103
MboII GAAGA 7 cut(s) 5, 265, 421, 686, 767, 1134, 1137
MfeI CAATTG 2 cut(s) 76, 294
MhlI GDGCHC 2 cut(s) 86, 990
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 4 cut(s) 118, 453, 754, 1107
MroXI GAANNNNTTC 1 cut(s) 690
MseI TTAA 3 cut(s) 252, 765, 852
MslI CAYNNNNRTG 1 cut(s) 501
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 4 cut(s) 328, 713, 813, 1156
MunI CAATTG 2 cut(s) 76, 294
MvaI CCWGG 4 cut(s) 328, 713, 813, 1156
MvnI CGCG 2 cut(s) 146, 310
MwoI GCNNNNNNNGC 2 cut(s) 420, 667
NdeII GATC 3 cut(s) 322, 1066, 1103
NlaIV GGNNCC 1 cut(s) 1184
NruI TCGCGA 1 cut(s) 146
NspV TTCGAA 3 cut(s) 128, 445, 684
PaqCI CACCTGC 1 cut(s) 523
PasI CCCWGGG 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 404
PcsI WCGNNNNNNNCGW 2 cut(s) 134, 143
PdmI GAANNNNTTC 1 cut(s) 690
PfeI GAWTC 8 cut(s) 232, 290, 335, 371, 436, 875, 890, 1151
PflMI CCANNNNNTGG 1 cut(s) 633
PfoI TCCNGGA 1 cut(s) 1154
PkrI GCNGC 3 cut(s) 612, 997, 1000
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 1183
PsiI TTATAA 1 cut(s) 806
Psp124BI GAGCTC 1 cut(s) 86
Psp1406I AACGTT 2 cut(s) 688, 785
Psp5II RGGWCCY 1 cut(s) 1183
Psp6I CCWGG 4 cut(s) 326, 711, 811, 1154
PspEI GGTNACC 1 cut(s) 1136
PspGI CCWGG 4 cut(s) 326, 711, 811, 1154
PspN4I GGNNCC 1 cut(s) 1184
PspPI GGNCC 3 cut(s) 201, 546, 1183
PspPPI RGGWCCY 1 cut(s) 1183
PstI CTGCAG 1 cut(s) 1000
PstNI CAGNNNCTG 1 cut(s) 869
RruI TCGCGA 1 cut(s) 146
RsaI GTAC 3 cut(s) 457, 845, 931
RsaNI GTAC 3 cut(s) 456, 844, 930
RseI CAYNNNNRTG 1 cut(s) 501
SacI GAGCTC 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 404
SaqAI TTAA 3 cut(s) 252, 765, 852
SatI GCNGC 3 cut(s) 611, 996, 999
Sau3AI GATC 3 cut(s) 322, 1066, 1103
Sau96I GGNCC 3 cut(s) 201, 546, 1183
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 4 cut(s) 328, 713, 813, 1156
SduI GDGCHC 2 cut(s) 86, 990
SfaNI GCATC 1 cut(s) 964
SfcI CTRYAG 2 cut(s) 870, 996
SfuI TTCGAA 3 cut(s) 128, 445, 684
SinI GGWCC 2 cut(s) 546, 1183
SmiMI CAYNNNNRTG 1 cut(s) 501
SsiI CCGC 5 cut(s) 38, 98, 381, 838, 1006
SspI AATATT 2 cut(s) 553, 1079
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 4 cut(s) 326, 711, 811, 1154
StyI CCWWGG 1 cut(s) 1023
TaaI ACNGT 1 cut(s) 848
TaiI ACGT 4 cut(s) 691, 788, 858, 957
TaqI TCGA 8 cut(s) 128, 137, 276, 445, 684, 699, 1071, 1149
TfiI GAWTC 8 cut(s) 232, 290, 335, 371, 436, 875, 890, 1151
Tru1I TTAA 3 cut(s) 252, 765, 852
Tru9I TTAA 3 cut(s) 252, 765, 852
TseI GCWGC 3 cut(s) 610, 995, 998
TspDTI ATGAA 4 cut(s) 336, 519, 882, 1089
TspGWI ACGGA 1 cut(s) 473
Van91I CCANNNNNTGG 1 cut(s) 633
VneI GTGCAC 1 cut(s) 986
VpaK11BI GGWCC 2 cut(s) 546, 1183
XapI RAATTY 9 cut(s) 139, 148, 163, 361, 487, 761, 884, 900, 1129
XcmI CCANNNNNNNNNTGG 3 cut(s) 346, 634, 1030
XmnI GAANNNNTTC 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.