MD12G1200800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017374
Physical Location & Seq
Reverse (-)
28140314 .. 28141105
792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1200800.v1.1.491

Sequence Viewer

Length: 225 bp
ATGTCCGTCTGCCCTTGTCCTACTTCGGGATACCTCGTCCGGGGCACGTTGGATCCCAATGTGTTGCAAAAGTTGATTCACCAAGGTTGTCTGTTGTGCAAGGGCGCTCGTCAACTCTATGACTTGTCGAGACAAGTGTTGTTCGCCATTTGGATTGGAAGAGCTTGGAAGGAATGCGCTTCCTTGGGCAGTGAAAGGGTGGTAGACTTCGGGCGCGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

75

Amino Acids

8.27

Weight (kDa)

8.73

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 216
AclWI GGATC 2 cut(s) 47, 60
AfiI CCNNNNNNNGG 2 cut(s) 26, 40
AluBI AGCT 1 cut(s) 164
AluI AGCT 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 2 cut(s) 47, 60
AspLEI GCGC 3 cut(s) 107, 179, 216
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 47
BamHI GGATCC 1 cut(s) 52
BciVI GTATCC 1 cut(s) 23
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 124
BfoI RGCGCY 1 cut(s) 108
BfuI GTATCC 1 cut(s) 23
Bme1390I CCNGG 1 cut(s) 41
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 41
BsaJI CCNNGG 3 cut(s) 40, 82, 183
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 40
BseDI CCNNGG 3 cut(s) 40, 82, 183
BseLI CCNNNNNNNGG 2 cut(s) 26, 40
BseSI GKGCMC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 216
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 2 cut(s) 26, 40
BsmAI GTCTC 1 cut(s) 124
BsmI GAATGC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 1 cut(s) 52
BspFNI CGCG 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 54
BspPI GGATC 2 cut(s) 47, 60
BspQI GCTCTTC 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 40, 82, 183
BssMI GATC 1 cut(s) 52
BssT1I CCWWGG 2 cut(s) 82, 183
Bst6I CTCTTC 1 cut(s) 154
BstFNI CGCG 1 cut(s) 216
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 3 cut(s) 107, 179, 216
BstKTI GATC 1 cut(s) 55
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 47
BstUI CGCG 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BsuI GTATCC 1 cut(s) 23
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 196
CfoI GCGC 3 cut(s) 107, 179, 216
CviJI RGCY 1 cut(s) 164
CviKI_1 RGCY 1 cut(s) 164
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 82, 183
EcoT14I CCWWGG 2 cut(s) 82, 183
ErhI CCWWGG 2 cut(s) 82, 183
FaiI YATR 1 cut(s) 120
FblI GTMKAC 1 cut(s) 204
GlaI GCGC 3 cut(s) 106, 178, 215
HaeII RGCGCY 1 cut(s) 108
HapII CCGG 1 cut(s) 40
HhaI GCGC 3 cut(s) 107, 179, 216
Hin6I GCGC 3 cut(s) 105, 177, 214
HinP1I GCGC 3 cut(s) 105, 177, 214
HincII GTYRAC 1 cut(s) 113
HindII GTYRAC 1 cut(s) 113
HinfI GANTC 1 cut(s) 76
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 2 cut(s) 113, 205
Hpy188III TCNNGA 2 cut(s) 27, 129
Hpy8I GTNNAC 2 cut(s) 113, 205
HpyAV CCTTC 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 2 cut(s) 67, 99
HpySE526I ACGT 1 cut(s) 47
HspAI GCGC 3 cut(s) 105, 177, 214
Kzo9I GATC 1 cut(s) 52
LguI GCTCTTC 1 cut(s) 154
LpnPI CCDG 1 cut(s) 53
MaeII ACGT 1 cut(s) 47
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 1 cut(s) 171
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 1 cut(s) 44
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 1 cut(s) 41
Mva1269I GAATGC 1 cut(s) 179
MvnI CGCG 1 cut(s) 216
NciI CCSGG 1 cut(s) 41
NdeII GATC 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 54
PciSI GCTCTTC 1 cut(s) 154
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 54
PsuI RGATCY 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 154
Sau3AI GATC 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 47
SetI ASST 4 cut(s) 36, 50, 88, 166
StyD4I CCNGG 1 cut(s) 39
StyI CCWWGG 2 cut(s) 82, 183
TaiI ACGT 1 cut(s) 50
TaqI TCGA 1 cut(s) 128
TfiI GAWTC 1 cut(s) 76
TscAI CASTG 1 cut(s) 196
TspRI CASTG 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.