pycom05g08120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0017374
Physical Location & Seq
Forward (+)
10856588 .. 10857881
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08120.8

Sequence Viewer

Length: 924 bp
ATGCATAATAGGCCAGTAGTAGCTTGCATTAAGAAGGGGGTGATGTGCCTTATATGTACATGCCTACCTCCATATAGCATGAGGCCTTTTGGGAGCTCATTGATTTCAGATTCCATCGGAACTTCGAAGTTAAGCGAGTTCAAACGAGAGCATTCCCAGGATGGATTGTGCTACGACGGAGTCGAGCCTAGGATGTGGTGGAGCCCGGGTCAGGATGTGACACCAAGGATTGGCTTTCAGAGTAATTTCCACAAACGCCTTTGTGGATTAAGCTTAGAACCTTTAGGTTGTCGAGAGGTGCTAGGCTGCGGAGATGTGGTCAAGTGTAGGATCTTAGGACAAGAATGTTGTTCCACATGTAGTAACGTGCTGCCTTTGTTTAGATCTATAGTGGAATCTTGCCAGTTTAGAGTTGTACTAACTTGTGACACCTCGACTGTTGGCTCCGCCATTATGCTTAGTTTAGGTCGGCCATTATGTTGGATAACGCATCTACGTGGATGTTGTCTGCCTATGGAACTTGAGTGGGGGTGTAAGTTTGAAACGCCTCAAGTTACTTTCATACATTCTCTAGGTATTGTGCCATCTTCGGATGTGTTGTCGTGTACTCCCCAATACTTTTTCATCACCAAGTCTTTTGCCATTCAGAGGCCTGCTACTAGAGCTTCGTACTCTGTTTCGTTGTTGGATGCTTCGAAGCCTAGAGTGATTGGCTGTTCAAGCATTGAACCGTCTAGGGTGACAAGGACCACGCCTGCTCCCGAGCCCTTGTATTGGGCGAAGCAGGCGTGGCTAAGGCATGCTCGGTTGCCTCCAGAGCGTTGTTGGGTTGCTCTGTTTGTGTCGCTTAGGCTAGGCTTGAATTCTACTATGAAGTCCGCCAAGTTTCAATGTCCATTTGATTATTCATTGAGAAGCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

34.25

Weight (kDa)

8.9

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 230
AciI CCGC 3 cut(s) 309, 447, 879
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 470
AcsI RAATTY 1 cut(s) 862
AfaI GTAC 4 cut(s) 58, 417, 607, 671
AfiI CCNNNNNNNGG 4 cut(s) 211, 230, 648, 774
AflIII ACRYGT 1 cut(s) 356
AgsI TTSAA 6 cut(s) 142, 542, 720, 728, 862, 890
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 4 cut(s) 23, 96, 273, 665
AluI AGCT 4 cut(s) 23, 96, 273, 665
Alw21I GWGCWC 1 cut(s) 98
AlwI GGATC 1 cut(s) 338
Ama87I CYCGRG 2 cut(s) 205, 761
AoxI GGCC 4 cut(s) 11, 83, 470, 650
ApeKI GCWGC 2 cut(s) 306, 370
ApoI RAATTY 1 cut(s) 862
AspA2I CCTAGG 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 747
AsuC2I CCSGG 2 cut(s) 206, 207
AsuHPI GGTGA 3 cut(s) 52, 619, 751
AsuII TTCGAA 2 cut(s) 125, 695
AvaI CYCGRG 2 cut(s) 205, 761
AvaII GGWCC 1 cut(s) 747
AvrII CCTAGG 1 cut(s) 188
BanII GRGCYC 3 cut(s) 98, 206, 768
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 2 cut(s) 293, 357
BccI CCATC 3 cut(s) 122, 155, 592
BciT130I CCWGG 1 cut(s) 158
BcnI CCSGG 2 cut(s) 206, 207
BfaI CTAG 8 cut(s) 189, 302, 572, 660, 702, 735, 854, 922
BfmI CTRYAG 1 cut(s) 387
BglII AGATCT 1 cut(s) 383
BisI GCNGC 2 cut(s) 307, 371
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 2 cut(s) 308, 372
Bme1390I CCNGG 3 cut(s) 158, 206, 207
Bme18I GGWCC 1 cut(s) 747
BmeT110I CYCGRG 2 cut(s) 205, 761
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 2 cut(s) 203, 445
BmrFI CCNGG 3 cut(s) 158, 206, 207
BmsI GCATC 2 cut(s) 499, 679
BpmI CTGGAG 1 cut(s) 798
Bpu10I CCTNAGC 2 cut(s) 794, 848
Bpu14I TTCGAA 2 cut(s) 125, 695
BpuEI CTTGAG 2 cut(s) 534, 542
BpuMI CCSGG 2 cut(s) 206, 207
BsaAI YACGTR 1 cut(s) 497
BsaJI CCNNGG 4 cut(s) 156, 188, 205, 224
BsaXI ACNNNNNCTCC 6 cut(s) 193, 223, 303, 333, 742, 772
Bsc4I CCNNNNNNNGG 4 cut(s) 211, 230, 648, 774
Bse1I ACTGG 2 cut(s) 14, 403
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 4 cut(s) 156, 188, 205, 224
BseGI GGATG 6 cut(s) 166, 198, 220, 506, 598, 694
BseLI CCNNNNNNNGG 4 cut(s) 211, 230, 648, 774
BseNI ACTGG 2 cut(s) 14, 403
BseXI GCAGC 2 cut(s) 293, 357
BshFI GGCC 4 cut(s) 13, 85, 472, 652
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 2 cut(s) 205, 761
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 4 cut(s) 211, 230, 648, 774
BsmI GAATGC 1 cut(s) 151
BsnI GGCC 4 cut(s) 13, 85, 472, 652
BsoBI CYCGRG 2 cut(s) 205, 761
Bsp119I TTCGAA 2 cut(s) 125, 695
Bsp1286I GDGCHC 3 cut(s) 98, 206, 768
Bsp1407I TGTACA 1 cut(s) 56
Bsp143I GATC 2 cut(s) 330, 383
BspACI CCGC 3 cut(s) 309, 447, 879
BspANI GGCC 4 cut(s) 13, 85, 472, 652
BspLI GGNNCC 2 cut(s) 203, 445
BspPI GGATC 1 cut(s) 338
BspT104I TTCGAA 2 cut(s) 125, 695
BsrGI TGTACA 1 cut(s) 56
BsrI ACTGG 2 cut(s) 14, 403
BssECI CCNNGG 4 cut(s) 156, 188, 205, 224
BssMI GATC 2 cut(s) 330, 383
BssT1I CCWWGG 2 cut(s) 188, 224
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 439, 732
BstAUI TGTACA 1 cut(s) 56
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 2 cut(s) 125, 695
BstC8I GCNNGC 5 cut(s) 25, 654, 756, 786, 801
BstDEI CTNAG 5 cut(s) 274, 334, 458, 794, 848
BstF5I GGATG 6 cut(s) 166, 198, 220, 506, 598, 694
BstKTI GATC 2 cut(s) 333, 386
BstMBI GATC 2 cut(s) 330, 383
BstMWI GCNNNNNNNGC 6 cut(s) 10, 662, 720, 785, 790, 817
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 3 cut(s) 63, 360, 803
BstSCI CCNGG 3 cut(s) 156, 204, 205
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 2 cut(s) 293, 357
BstX2I RGATCY 2 cut(s) 330, 383
BstXI CCANNNNNNTGG 1 cut(s) 480
BstYI RGATCY 2 cut(s) 330, 383
BsuRI GGCC 4 cut(s) 13, 85, 472, 652
BtsCI GGATG 6 cut(s) 166, 198, 220, 506, 598, 694
Cac8I GCNNGC 5 cut(s) 25, 654, 756, 786, 801
Cfr13I GGNCC 1 cut(s) 747
Cfr9I CCCGGG 1 cut(s) 205
Csp6I GTAC 4 cut(s) 57, 416, 606, 670
CviAII CATG 4 cut(s) 60, 79, 357, 800
CviQI GTAC 4 cut(s) 57, 416, 606, 670
DdeI CTNAG 5 cut(s) 274, 334, 458, 794, 848
DpnI GATC 2 cut(s) 332, 385
DpnII GATC 2 cut(s) 330, 383
EaeI YGGCCR 1 cut(s) 470
EciI GGCGGA 2 cut(s) 436, 868
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 188, 224
Eco147I AGGCCT 2 cut(s) 85, 652
Eco24I GRGCYC 3 cut(s) 98, 206, 768
Eco47I GGWCC 1 cut(s) 747
Eco53kI GAGCTC 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 205, 761
EcoICRI GAGCTC 1 cut(s) 96
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 1 cut(s) 156
EcoT14I CCWWGG 2 cut(s) 188, 224
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 98, 206, 768
ErhI CCWWGG 2 cut(s) 188, 224
FaeI CATG 4 cut(s) 63, 82, 360, 803
FatI CATG 4 cut(s) 59, 78, 356, 799
Fnu4HI GCNGC 2 cut(s) 307, 371
FokI GGATG 6 cut(s) 173, 205, 227, 513, 605, 701
FriOI GRGCYC 3 cut(s) 98, 206, 768
Fsp4HI GCNGC 2 cut(s) 307, 371
FspBI CTAG 8 cut(s) 189, 302, 572, 660, 702, 735, 854, 922
GluI GCNGC 2 cut(s) 307, 371
GsuI CTGGAG 1 cut(s) 798
HaeIII GGCC 4 cut(s) 13, 85, 472, 652
HapII CCGG 1 cut(s) 206
Hin1II CATG 4 cut(s) 63, 82, 360, 803
HindIII AAGCTT 1 cut(s) 271
HinfI GANTC 3 cut(s) 110, 180, 395
HpaII CCGG 1 cut(s) 206
HphI GGTGA 3 cut(s) 52, 619, 751
Hpy166II GTNNAC 1 cut(s) 606
Hpy188I TCNGA 5 cut(s) 109, 119, 240, 592, 648
Hpy188III TCNNGA 4 cut(s) 212, 293, 761, 815
Hpy8I GTNNAC 1 cut(s) 606
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 439, 732
HpyCH4IV ACGT 2 cut(s) 366, 496
HpyCH4V TGCA 2 cut(s) 4, 27
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 662, 720, 785, 790, 817
HpyF3I CTNAG 5 cut(s) 274, 334, 458, 794, 848
HpySE526I ACGT 2 cut(s) 366, 496
Hsp92II CATG 4 cut(s) 63, 82, 360, 803
Kzo9I GATC 2 cut(s) 330, 383
LmnI GCTCC 4 cut(s) 93, 201, 449, 763
Lsp1109I GCAGC 2 cut(s) 293, 357
LweI GCATC 2 cut(s) 499, 679
MaeI CTAG 8 cut(s) 189, 302, 572, 660, 702, 735, 854, 922
MaeII ACGT 2 cut(s) 366, 496
MaeIII GTNAC 5 cut(s) 217, 362, 425, 553, 739
MalI GATC 2 cut(s) 332, 385
MboI GATC 2 cut(s) 330, 383
MboII GAAGA 1 cut(s) 579
MflI RGATCY 2 cut(s) 330, 383
MhlI GDGCHC 3 cut(s) 98, 206, 768
MluCI AATT 2 cut(s) 244, 862
MlyI GAGTC 1 cut(s) 189
MmeI TCCRAC 2 cut(s) 461, 666
MnlI CCTC 7 cut(s) 75, 78, 289, 442, 558, 642, 822
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 30, 131, 269
MslI CAYNNNNRTG 1 cut(s) 495
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 3 cut(s) 158, 206, 207
Mva1269I GAATGC 1 cut(s) 151
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 6 cut(s) 10, 662, 720, 785, 790, 817
NciI CCSGG 2 cut(s) 206, 207
NdeII GATC 2 cut(s) 330, 383
NlaIII CATG 4 cut(s) 63, 82, 360, 803
NlaIV GGNNCC 2 cut(s) 203, 445
NmuCI GTSAC 3 cut(s) 217, 425, 739
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 63, 360, 803
NspV TTCGAA 2 cut(s) 125, 695
PaeI GCATGC 1 cut(s) 803
PceI AGGCCT 2 cut(s) 85, 652
PciI ACATGT 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 151
PfeI GAWTC 2 cut(s) 110, 395
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 2 cut(s) 308, 372
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 497
PscI ACATGT 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 203, 445
PspPI GGNCC 1 cut(s) 747
PsuI RGATCY 2 cut(s) 330, 383
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 4 cut(s) 58, 417, 607, 671
RsaNI GTAC 4 cut(s) 57, 416, 606, 670
RseI CAYNNNNRTG 1 cut(s) 495
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 30, 131, 269
SatI GCNGC 2 cut(s) 307, 371
Sau3AI GATC 2 cut(s) 330, 383
Sau96I GGNCC 1 cut(s) 747
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 3 cut(s) 158, 206, 207
SduI GDGCHC 3 cut(s) 98, 206, 768
SfaNI GCATC 2 cut(s) 499, 679
SfcI CTRYAG 1 cut(s) 387
SfuI TTCGAA 2 cut(s) 125, 695
SinI GGWCC 1 cut(s) 747
SmaI CCCGGG 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 2 cut(s) 521, 549
SmoI CTYRAG 2 cut(s) 521, 549
SphI GCATGC 1 cut(s) 803
Sse9I AATT 2 cut(s) 244, 862
SseBI AGGCCT 2 cut(s) 85, 652
SsiI CCGC 3 cut(s) 309, 447, 879
SspMI CTAG 8 cut(s) 189, 302, 572, 660, 702, 735, 854, 922
SstI GAGCTC 1 cut(s) 98
StuI AGGCCT 2 cut(s) 85, 652
StyD4I CCNGG 3 cut(s) 156, 204, 205
StyI CCWWGG 2 cut(s) 188, 224
TaaI ACNGT 2 cut(s) 439, 732
TaiI ACGT 2 cut(s) 369, 499
TaqI TCGA 5 cut(s) 125, 183, 292, 434, 695
TasI AATT 2 cut(s) 244, 862
TatI WGTACW 3 cut(s) 56, 415, 605
TfiI GAWTC 2 cut(s) 110, 395
Tru1I TTAA 3 cut(s) 30, 131, 269
Tru9I TTAA 3 cut(s) 30, 131, 269
TseFI GTSAC 3 cut(s) 217, 425, 739
TseI GCWGC 2 cut(s) 306, 370
Tsp45I GTSAC 3 cut(s) 217, 425, 739
TspDTI ATGAA 4 cut(s) 550, 613, 887, 897
TspGWI ACGGA 1 cut(s) 192
TspMI CCCGGG 1 cut(s) 205
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 747
XapI RAATTY 1 cut(s) 862
XceI RCATGY 3 cut(s) 63, 360, 803
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XmaI CCCGGG 1 cut(s) 205
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 8 cut(s) 189, 302, 572, 660, 702, 735, 854, 922
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.