pycom12g04950

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017374
Physical Location & Seq
Forward (+)
4916855 .. 4918426
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04950.1

Sequence Viewer

Length: 1263 bp
ATGGCAACATGTACTCGATACTTGCTTGTTTATTGTTTACAGGATAGTCATGGCTCTAAGAAATACTATTGCCTAGTTTCATCATCACGATGCTTTGCTTTGGTGCTTGTTCTGCTACCGTTTATACAGTTGGTGGGGATGAAGGCAAAAGAATTTCACGTCGACCATGAATTCATACTTAACTACGAACATGAAGACGATTTTTTGACATTAGTGACTAGAGAAGTAGATTACAATCATTGCATTTCACCATCACTTGCCCTATCTGATTATGGTGGCTTTGCCACCTTAACTTTTAACATACCAAACAACTACTATGTTATTTTGCCGAACCTGATCACTACCAAGCCGGACAATAGATTAAAATCAAGATCAATACCCCGAACTAGAAAATTCAATCTTAAGTCCAATCCCCTTTTCTTCTCTATCCTCACTGTGGATGCAAATTTCCGTCTTCTTCTTCTTGGACAAAATTGTACCTACAAAACAAGTAACACCTTAGGTCAAGGCCAAGAAGCCTCCAATGCCAAAGTTAGAATTTTGAGGGAAAAGTGTTTGGAGAACTTGGAGAATTTTGCAAGAGAGTTGGAATTGAGTTTTGGAGAAAATGAGGGTGTTTATTTAGGGGTGTGGCCGGCCCTTTTAGGAGGAGATGGACCGGCCACTTGGATGGTTTTTTGGGTGAGATTCATGATTTGCAATAATATGAAAGGAAAGACTTGGGGGTTACATTGTGGAGGATATTTGATGAGGATGGATGAAATAGTTCCGGTATTCCTCGAGGTATTTTTGGCTCTACACTCACCATTAGGTTCTCCTTTTAATGCCTGGCTTGGCTGTTACCGGTCTTATGATTTTAATGTTTCAAATGCAGATCCAAAAAAAATAAAAAAATTCAGGTTGAATGTAAAAGTTGTGAATGAATTTGTATTAGCAATAGTTGAATTTATTTTCTTCGGATTGATTATAAATGAATTTTTACTATGTTTCATTAATTCTAGTTGTTTGCAATATATTATTTATATGATTTTTAATATGAGATCAGACATAACAACAAAATGCAAACTGATGAAACATGAACAGAATCGTAAAACTCCTTTGCTATACAATGACACAGATCGGATCCAATTAAATTCATCTCTTGTGAAATTATATTGCACATTTTTTTTTCACCCACTGGAAATACCATGGCTTCAAATGAAAAAAAAAAAAAAAAAAAAAAAAAATGCAGAAACCCGAACAAAAATTCTTAGACATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

421

Amino Acids

48.77

Weight (kDa)

9.15

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 968
AbsI CCTCGAGG 1 cut(s) 779
AccI GTMKAC 1 cut(s) 162
AclWI GGATC 3 cut(s) 869, 1117, 1130
AcoI YGGCCR 2 cut(s) 632, 660
AfaI GTAC 2 cut(s) 13, 478
AfiI CCNNNNNNNGG 1 cut(s) 436
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 8
AgeI ACCGGT 1 cut(s) 843
AgsI TTSAA 5 cut(s) 397, 867, 904, 944, 1196
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 1 cut(s) 827
AjuI GAANNNNNNNTTGG 4 cut(s) 401, 433, 582, 614
AlwI GGATC 3 cut(s) 869, 1117, 1130
Ama87I CYCGRG 1 cut(s) 779
AoxI GGCC 4 cut(s) 508, 632, 636, 660
AseI ATTAAT 1 cut(s) 993
AsiGI ACCGGT 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 765
AspS9I GGNCC 2 cut(s) 637, 656
AsuHPI GGTGA 4 cut(s) 240, 694, 795, 1163
AvaI CYCGRG 1 cut(s) 779
AvaII GGWCC 1 cut(s) 656
AxyI CCTNAGG 1 cut(s) 499
BamHI GGATCC 1 cut(s) 1122
BbsI GAAGAC 2 cut(s) 201, 446
BccI CCATC 4 cut(s) 259, 647, 664, 748
BciT130I CCWGG 1 cut(s) 829
BclI TGATCA 1 cut(s) 336
BfaI CTAG 4 cut(s) 74, 219, 387, 999
BfrI CTTAAG 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 829
Bme18I GGWCC 1 cut(s) 656
BmeT110I CYCGRG 1 cut(s) 779
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 637, 656
BmiI GGNNCC 1 cut(s) 1124
BmrFI CCNGG 1 cut(s) 829
BmsI GCATC 2 cut(s) 80, 430
BpiI GAAGAC 2 cut(s) 201, 446
BsaBI GATNNNNATC 2 cut(s) 234, 364
BsaJI CCNNGG 1 cut(s) 1187
BsaWI WCCGGW 2 cut(s) 769, 843
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse118I RCCGGY 3 cut(s) 634, 658, 843
Bse1I ACTGG 1 cut(s) 1182
Bse21I CCTNAGG 1 cut(s) 499
Bse3DI GCAATG 1 cut(s) 238
Bse8I GATNNNNATC 2 cut(s) 234, 364
BseBI CCWGG 1 cut(s) 829
BseDI CCNNGG 1 cut(s) 1187
BseGI GGATG 5 cut(s) 144, 445, 675, 759, 763
BseJI GATNNNNATC 2 cut(s) 234, 364
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 238
BseNI ACTGG 1 cut(s) 1182
BseRI GAGGAG 1 cut(s) 663
BshFI GGCC 4 cut(s) 510, 634, 638, 662
BshTI ACCGGT 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 779
BsiSI CCGG 5 cut(s) 350, 635, 659, 770, 844
BslI CCNNNNNNNGG 1 cut(s) 436
BsnI GGCC 4 cut(s) 510, 634, 638, 662
BsoBI CYCGRG 1 cut(s) 779
Bsp143I GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
Bsp19I CCATGG 1 cut(s) 1187
BspANI GGCC 4 cut(s) 510, 634, 638, 662
BspHI TCATGA 1 cut(s) 690
BspLI GGNNCC 1 cut(s) 1124
BspPI GGATC 3 cut(s) 869, 1117, 1130
BspTI CTTAAG 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 3 cut(s) 634, 658, 843
BsrI ACTGG 1 cut(s) 1182
BssAI RCCGGY 3 cut(s) 634, 658, 843
BssECI CCNNGG 1 cut(s) 1187
BssMI GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
BssT1I CCWWGG 1 cut(s) 1187
Bst2UI CCWGG 1 cut(s) 829
Bst4CI ACNGT 3 cut(s) 120, 129, 436
BstAFI CTTAAG 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 3 cut(s) 57, 499, 1250
BstDSI CCRYGG 1 cut(s) 1187
BstF5I GGATG 5 cut(s) 144, 445, 675, 759, 763
BstKTI GATC 6 cut(s) 339, 374, 877, 1043, 1120, 1125
BstMBI GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
BstMWI GCNNNNNNNGC 2 cut(s) 112, 524
BstNI CCWGG 1 cut(s) 829
BstNSI RCATGY 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 827
BstV2I GAAGAC 2 cut(s) 201, 446
BstX2I RGATCY 2 cut(s) 874, 1122
BstXI CCANNNNNNTGG 1 cut(s) 670
BstYI RGATCY 2 cut(s) 874, 1122
Bsu36I CCTNAGG 1 cut(s) 499
BsuRI GGCC 4 cut(s) 510, 634, 638, 662
BtgI CCRYGG 1 cut(s) 1187
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 5 cut(s) 144, 445, 675, 759, 763
BtsIMutI CAGTG 2 cut(s) 432, 1175
Cac8I GCNNGC 1 cut(s) 636
CciI TCATGA 1 cut(s) 690
Cfr10I RCCGGY 3 cut(s) 634, 658, 843
Cfr13I GGNCC 2 cut(s) 637, 656
Csp6I GTAC 2 cut(s) 12, 477
CspAI ACCGGT 1 cut(s) 843
CviAII CATG 7 cut(s) 9, 50, 167, 191, 691, 1076, 1188
CviQI GTAC 2 cut(s) 12, 477
DdeI CTNAG 3 cut(s) 57, 499, 1250
DpnI GATC 6 cut(s) 338, 373, 876, 1042, 1119, 1124
DpnII GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
EaeI YGGCCR 2 cut(s) 632, 660
Eco130I CCWWGG 1 cut(s) 1187
Eco47I GGWCC 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 499
Eco88I CYCGRG 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 827
EcoT14I CCWWGG 1 cut(s) 1187
ErhI CCWWGG 1 cut(s) 1187
FaeI CATG 7 cut(s) 12, 53, 170, 194, 694, 1079, 1191
FatI CATG 7 cut(s) 8, 49, 166, 190, 690, 1075, 1187
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 1 cut(s) 162
FokI GGATG 5 cut(s) 151, 452, 682, 766, 770
FseI GGCCGGCC 1 cut(s) 638
FspBI CTAG 4 cut(s) 74, 219, 387, 999
HaeIII GGCC 4 cut(s) 510, 634, 638, 662
HapII CCGG 5 cut(s) 350, 635, 659, 770, 844
Hin1II CATG 7 cut(s) 12, 53, 170, 194, 694, 1079, 1191
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 2 cut(s) 687, 1084
HpaII CCGG 5 cut(s) 350, 635, 659, 770, 844
HphI GGTGA 4 cut(s) 240, 694, 795, 1163
Hpy166II GTNNAC 2 cut(s) 38, 163
Hpy188I TCNGA 4 cut(s) 268, 959, 1045, 1122
Hpy188III TCNNGA 3 cut(s) 87, 369, 691
Hpy8I GTNNAC 2 cut(s) 38, 163
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 3 cut(s) 120, 129, 436
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 9 cut(s) 243, 443, 578, 699, 872, 1009, 1062, 1158, 1229
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 524
HpyF3I CTNAG 3 cut(s) 57, 499, 1250
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 7 cut(s) 12, 53, 170, 194, 694, 1079, 1191
KroI GCCGGC 1 cut(s) 634
KroNI GCCGGC 1 cut(s) 636
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
LweI GCATC 2 cut(s) 80, 430
MaeI CTAG 4 cut(s) 74, 219, 387, 999
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 4 cut(s) 214, 491, 726, 839
MalI GATC 6 cut(s) 338, 373, 876, 1042, 1119, 1124
MboI GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
MboII GAAGA 6 cut(s) 206, 412, 446, 449, 452, 946
MflI RGATCY 2 cut(s) 874, 1122
MmeI TCCRAC 1 cut(s) 567
MnlI CCTC 9 cut(s) 440, 529, 537, 604, 641, 731, 744, 775, 788
MroNI GCCGGC 1 cut(s) 634
MroXI GAANNNNTTC 1 cut(s) 765
MslI CAYNNNNRTG 2 cut(s) 88, 668
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 5 cut(s) 350, 635, 659, 770, 844
MspR9I CCNGG 1 cut(s) 829
MvaI CCWGG 1 cut(s) 829
MwoI GCNNNNNNNGC 2 cut(s) 112, 524
NaeI GCCGGC 1 cut(s) 636
NcoI CCATGG 1 cut(s) 1187
NdeII GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
NgoMIV GCCGGC 1 cut(s) 634
NlaIII CATG 7 cut(s) 12, 53, 170, 194, 694, 1079, 1191
NlaIV GGNNCC 1 cut(s) 1124
NmuCI GTSAC 1 cut(s) 214
NspI RCATGY 1 cut(s) 12
PaeR7I CTCGAG 1 cut(s) 779
PagI TCATGA 1 cut(s) 690
PciI ACATGT 1 cut(s) 8
PdiI GCCGGC 1 cut(s) 636
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 2 cut(s) 687, 1084
PinAI ACCGGT 1 cut(s) 843
PscI ACATGT 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 993
PsiI TTATAA 1 cut(s) 968
Psp6I CCWGG 1 cut(s) 827
PspGI CCWGG 1 cut(s) 827
PspN4I GGNNCC 1 cut(s) 1124
PspPI GGNCC 2 cut(s) 637, 656
PspXI VCTCGAGB 1 cut(s) 779
PsuI RGATCY 2 cut(s) 874, 1122
RigI GGCCGGCC 1 cut(s) 638
RsaI GTAC 2 cut(s) 13, 478
RsaNI GTAC 2 cut(s) 12, 477
RseI CAYNNNNRTG 2 cut(s) 88, 668
SalI GTCGAC 1 cut(s) 161
Sau3AI GATC 6 cut(s) 336, 371, 874, 1040, 1117, 1122
Sau96I GGNCC 2 cut(s) 637, 656
ScrFI CCNGG 1 cut(s) 829
SetI ASST 9 cut(s) 162, 290, 336, 482, 500, 505, 786, 814, 902
SfaNI GCATC 2 cut(s) 80, 430
Sfr274I CTCGAG 1 cut(s) 779
SinI GGWCC 1 cut(s) 656
SlaI CTCGAG 1 cut(s) 779
SmiMI CAYNNNNRTG 2 cut(s) 88, 668
SmlI CTYRAG 2 cut(s) 401, 779
SmoI CTYRAG 2 cut(s) 401, 779
SspMI CTAG 4 cut(s) 74, 219, 387, 999
StyD4I CCNGG 1 cut(s) 827
StyI CCWWGG 1 cut(s) 1187
TaaI ACNGT 3 cut(s) 120, 129, 436
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 16, 162, 780
TatI WGTACW 1 cut(s) 11
TfiI GAWTC 2 cut(s) 687, 1084
TscAI CASTG 2 cut(s) 439, 1182
TseFI GTSAC 1 cut(s) 214
Tsp45I GTSAC 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 2 cut(s) 439, 1182
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 656
VspI ATTAAT 1 cut(s) 993
XceI RCATGY 1 cut(s) 12
XhoI CTCGAG 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 4 cut(s) 74, 219, 387, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.